Restriction map for pF1KB5346
 (Linear) (Six-base) MAPSORT of: KIBB5346.seq  Check: 3474  from: 1  to: 1854



KIBB5346.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:27  ..

AatII G_ACGT'C

Cuts at:      0    1428    1854 
   Size:       1428     426
 
AclI AA'CG_TT

Cuts at:      0    1453    1854 
   Size:       1453     401
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     178     722    1310    1759    1854 
   Size:        178     544     588     449      95

  Fragments arranged by size: 

                 588     544     449     178      95
 
AflIII A'CryG_T

Cuts at:      0      11    1854 
   Size:         11    1843
 
AhdI GACnn_n'nnGTC

Cuts at:      0      12    1854 
   Size:         12    1842
 
AleI CACnn'nnGTG

Cuts at:      0      22     159     587    1854 
   Size:         22     137     428    1267

  Fragments arranged by size: 

                1267     428     137      22
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     605    1854 
   Size:        605    1249
 
AlwNI CAG_nnn'CTG

Cuts at:      0     102     315     390     717    1854 
   Size:        102     213      75     327    1137

  Fragments arranged by size: 

                1137     327     213     102      75
 
ApaLI G'TGCA_C

Cuts at:      0    1229    1854 
   Size:       1229     625
 
ApoI r'AATT_y

Cuts at:      0    1435    1854 
   Size:       1435     419
 
AvaI C'yCGr_G

Cuts at:      0    1096    1777    1854 
   Size:       1096     681      77

  Fragments arranged by size: 

                1096     681      77
 
BanII G_rGCy'C

Cuts at:      0     242     969    1095    1854 
   Size:        242     727     126     759

  Fragments arranged by size: 

                 759     727     242     126
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      44     557     739    1844    1854 
   Size:         44     513     182    1105      10

  Fragments arranged by size: 

                1105     513     182      44      10
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     313     347    1854 
   Size:        313      34    1507

  Fragments arranged by size: 

                1507     313      34
 
BfrBI ATG'CAT

Cuts at:      0    1284    1854 
   Size:       1284     570
 
BglII A'GATC_T

Cuts at:      0    1100    1854 
   Size:       1100     754
 
Bme1580I G_kGCm'C

Cuts at:      0     701    1233    1416    1854 
   Size:        701     532     183     438

  Fragments arranged by size: 

                 701     532     438     183
 
BmgBI CAC'GTC

Cuts at:      0     759    1584    1854 
   Size:        759     825     270

  Fragments arranged by size: 

                 825     759     270
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1126    1854 
   Size:       1126     728
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     169     247     442     463     739     748    1854 
   Size:        169      78     195      21     276       9    1106

  Fragments arranged by size: 

                1106     276     195     169      78      21       9
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1481    1854 
   Size:       1481     373
 
BsaHI Gr'CG_yC

Cuts at:      0    1425    1783    1841    1854 
   Size:       1425     358      58      13

  Fragments arranged by size: 

                1425     358      58      13
 
BsaWI w'CCGG_w

Cuts at:      0     215    1854 
   Size:        215    1639
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     337     367    1854 
   Size:        337      30    1487

  Fragments arranged by size: 

                1487     337      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     211     439     442    1854 
   Size:        211     228       3    1412

  Fragments arranged by size: 

                1412     228     211       3
 
BseYI C'CCAG_C

Cuts at:      0     605    1854 
   Size:        605    1249
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     611    1854 
   Size:        611    1243
 
BsiHKAI G_wGCw'C

Cuts at:      0     125     242     969    1233    1529    1854 
   Size:        125     117     727     264     296     325

  Fragments arranged by size: 

                 727     325     296     264     125     117
 
BsmI GAATG_Cn'

Cuts at:      0     294    1309    1854 
   Size:        294    1015     545

  Fragments arranged by size: 

                1015     545     294
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1790    1854 
   Size:       1790      64
 
Bsp1286I G_dGCh'C

Cuts at:      0     125     242     701     969    1095    1233    1416    1529 
   Size:        125     117     459     268     126     138     183     113

Cuts at:   1529    1854 
   Size:        325

  Fragments arranged by size: 

                 459     325     268     183     138     126     125     117
                 113
 
BspEI T'CCGG_A

Cuts at:      0     215    1854 
   Size:        215    1639
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     315    1854 
   Size:        315    1539
 
BsrGI T'GTAC_A

Cuts at:      0      26     791    1208    1854 
   Size:         26     765     417     646

  Fragments arranged by size: 

                 765     646     417      26
 
BssSI C'ACGA_G

Cuts at:      0    1226    1754    1854 
   Size:       1226     528     100

  Fragments arranged by size: 

                1226     528     100
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     315     390    1854 
   Size:        315      75    1464

  Fragments arranged by size: 

                1464     315      75
 
BstYI r'GATC_y

Cuts at:      0     623    1100    1660    1854 
   Size:        623     477     560     194

  Fragments arranged by size: 

                 623     560     477     194
 
BtgI C'CryG_G

Cuts at:      0     596    1854 
   Size:        596    1258
 
BtsI GCAGTG_nn'

Cuts at:      0     594    1680    1722    1833    1854 
   Size:        594    1086      42     111      21

  Fragments arranged by size: 

                1086     594     111      42      21
 
DraI TTT'AAA

Cuts at:      0    1667    1854 
   Size:       1667     187
 
EaeI y'GGCC_r

Cuts at:      0     371     761    1790    1854 
   Size:        371     390    1029      64

  Fragments arranged by size: 

                1029     390     371      64
 
EarI CTCTTCn'nnn_

Cuts at:      0     391    1854 
   Size:        391    1463
 
EcoICRI GAG'CTC

Cuts at:      0     240     967    1854 
   Size:        240     727     887

  Fragments arranged by size: 

                 887     727     240
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     169     178     247     442     463     722     739     748 
   Size:        169       9      69     195      21     259      17       9

Cuts at:    748    1310    1759    1854 
   Size:        562     449      95

  Fragments arranged by size: 

                 562     449     259     195     169      95      69      21
                  17       9       9
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     250    1610    1854 
   Size:        250    1360     244

  Fragments arranged by size: 

                1360     250     244
 
EcoO109I rG'GnC_Cy

Cuts at:      0     714     723    1120    1854 
   Size:        714       9     397     734

  Fragments arranged by size: 

                 734     714     397       9
 
FspI TGC'GCA

Cuts at:      0     323    1854 
   Size:        323    1531
 
HaeII r_GCGC'y

Cuts at:      0     684    1854 
   Size:        684    1170
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      30     187     219    1209    1241    1315    1347    1854 
   Size:         30     157      32     990      32      74      32     507

  Fragments arranged by size: 

                 990     507     157      74      32      32      32      30
            
 
HindIII A'AGCT_T

Cuts at:      0      52     107    1854 
   Size:         52      55    1747

  Fragments arranged by size: 

                1747      55      52
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      26     215    1405    1854 
   Size:         26     189    1190     449

  Fragments arranged by size: 

                1190     449     189      26
 
MscI TGG'CCA

Cuts at:      0     373     763    1854 
   Size:        373     390    1091

  Fragments arranged by size: 

                1091     390     373
 
MslI CAynn'nnrTG

Cuts at:      0      22     159     287     587    1007    1279    1854 
   Size:         22     137     128     300     420     272     575

  Fragments arranged by size: 

                 575     420     300     272     137     128      22
 
MspA1I CmG'CkG

Cuts at:      0     598    1854 
   Size:        598    1256
 
NsiI A_TGCA'T

Cuts at:      0    1286    1854 
   Size:       1286     568
 
NspI r_CATG'y

Cuts at:      0      15    1288    1854 
   Size:         15    1273     566

  Fragments arranged by size: 

                1273     566      15
 
PasI CC'CwG_GG

Cuts at:      0     559    1854 
   Size:        559    1295
 
PciI A'CATG_T

Cuts at:      0      11    1854 
   Size:         11    1843
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     654    1854 
   Size:        654    1200
 
PpuMI rG'GwC_Cy

Cuts at:      0     723    1120    1854 
   Size:        723     397     734

  Fragments arranged by size: 

                 734     723     397
 
PstI C_TGCA'G

Cuts at:      0     395    1449    1854 
   Size:        395    1054     405

  Fragments arranged by size: 

                1054     405     395
 
SacI G_AGCT'C

Cuts at:      0     242     969    1854 
   Size:        242     727     885

  Fragments arranged by size: 

                 885     727     242
 
SacII CC_GC'GG

Cuts at:      0     599    1854 
   Size:        599    1255
 
SexAI A'CCwGG_T

Cuts at:      0     653    1854 
   Size:        653    1201
 
SfcI C'TryA_G

Cuts at:      0     391    1253    1445    1854 
   Size:        391     862     192     409

  Fragments arranged by size: 

                 862     409     391     192
 
SmaI CCC'GGG

Cuts at:      0    1779    1854 
   Size:       1779      75
 
SmlI C'TyrA_G

Cuts at:      0    1096    1854 
   Size:       1096     758
 
SphI G_CATG'C

Cuts at:      0    1288    1854 
   Size:       1288     566
 
SspI AAT'ATT

Cuts at:      0    1078    1854 
   Size:       1078     776
 
StyI C'CwwG_G

Cuts at:      0     785     842    1407    1854 
   Size:        785      57     565     447

  Fragments arranged by size: 

                 785     565     447      57
 
TatI w'GTAC_w

Cuts at:      0      26     791     935    1208    1854 
   Size:         26     765     144     273     646

  Fragments arranged by size: 

                 765     646     273     144      26
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     337     369    1854 
   Size:        337      32    1485

  Fragments arranged by size: 

                1485     337      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1585    1854 
   Size:       1585     269
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     522    1854 
   Size:        522    1332
 
XhoI C'TCGA_G

Cuts at:      0    1096    1854 
   Size:       1096     758
 
XmaI C'CCGG_G

Cuts at:      0    1777    1854 
   Size:       1777      77
 
ZraI GAC'GTC

Cuts at:      0    1426    1854 
   Size:       1426     428
 

 Enzymes that do cut:

    AatII     AclI     AcuI   AflIII     AhdI     AleI     AlfI    AlwNI
    ApaLI     ApoI     AvaI    BanII     BbsI     BcgI    BfrBI    BglII
 Bme1580I    BmgBI     BmrI     BpmI     BsaI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI
    BspMI    BsrGI    BssSI   BstAPI    BstYI     BtgI     BtsI     DraI
     EaeI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I     FspI    HaeII
    Hin4I  HindIII     MmeI     MscI     MslI   MspA1I     NsiI     NspI
     PasI     PciI    PflMI    PpuMI     PstI     SacI    SacII    SexAI
     SfcI     SmaI     SmlI     SphI     SspI     StyI     TatI     TstI
  Tth111I     XcmI     XhoI     XmaI     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AfeI    AflII     AgeI     AloI     ApaI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI
    BbvCI    BciVI     BclI     BglI     BlpI     BmtI     BplI   Bpu10I
    BpuEI    BsaAI    BsaBI    BsiEI    BsiWI    BspHI    BsrBI    BsrDI
    BsrFI   BssHII    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI
     ClaI    CspCI   DraIII     DrdI     EagI     EciI    EcoRI    EcoRV
     FalI     FseI    FspAI   HincII     HpaI     KasI     KpnI     MfeI
     MluI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     PacI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SalI    SanDI
     SapI     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI    SnaBI
     SpeI     SrfI     StuI     SwaI    TaqII     XbaI     XmnI
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