Restriction map for pF1KB5341
##################################
# NO CUT ENZYMES (  155 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AflII       AflIII      
AgeI        AhdI        Alw44I      Aor51HI     ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AviII       AvrII       BalI        
BamHI       BanI        BbeI        BbrPI       BbvCI       
BcgI        BclI        BfrI        BglI        BlnI        
BlpI        Bpu1102I    BsaAI       BsaBI       BsaHI       
BsaI        BsaWI       BseAI       BsgI        BsiEI       
BsiWI       BsmBI       BsmI        Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrBI       BsrDI       
BsrGI       BssHII      BssSI       Bst1107I    BstBI       
BstEII      BstPI       BstUI       BstXI       BstZ17I     
CelII       ClaI        CpoI        DraI        DraIII      
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoNI       EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        FseI        FspI        HaeII       
HgaI        Hin1I       HindIII     HpaI        HphI        
KasI        KpnI        KspI        MaeII       MamI        
MfeI        MluI        MluNI       MroI        MscI        
MslI        MunI        MvnI        NaeI        NarI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflMI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RcaI        RsrII       SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfcI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        Van91I      XbaI        
XcmI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   35 enzymes) #
###################################

AlwNI         876  CAGGTACTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          862  CCTGAGG              CC^T[ACGT]AGG
ApaI          571  GGGCCC               GGGCC^C
AspI          868  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          110  CTCGGG               C^[CT]CG[GA]G
AvaII         963  GGACC                G^G[AT]CC
BbsI          634  GTCTTC               GAAGAC(2/6)
BciVI         487  GGATAC               GTATCC(6/5)
BcnI          226  CCCGG                CC^[GC]GG
BfaI          505  CTAG                 C^TAG
BglII         431  AGATCT               A^GATCT
BpuAI         634  GTCTTC               GAAGAC(2/6)
BsoBI         110  CTCGGG               C^[CT]CG[GA]G
BspMI         854  ACCTGC               ACCTGC(4/8)
BsrFI          59  ACCGGC               [GA]^CCGG[CT]
BstAPI        685  GCAAAGGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I        862  CCTGAGG              CC^T[ACGT]AGG
CfoI          702  GCGC                 GCG^C
Cfr10I         59  ACCGGC               [GA]^CCGG[CT]
EaeI          261  TGGCCG               [CT]^GGCC[GA]
EarI          761  GAAGAG               CTCTTC(1/4)
Eco81I        862  CCTGAGG              CC^T[ACGT]AGG
HhaI          702  GCGC                 GCG^C
HinP1I        702  GCGC                 G^CGC
HincII        424  GTTGAC               GT[CT]^[GA]AC
HindII        424  GTTGAC               GT[CT]^[GA]AC
Ksp632I       761  GAAGAG               CTCTTC(1/4)
MaeI          505  CTAG                 C^TAG
MspA1I        958  CAGCGG               C[AC]G^C[GT]G
NciI          226  CCCGG                CC^[GC]GG
NdeI          782  CATATG               CA^TATG
PflFI         868  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI         962  GGGACCC              [GA]G^G[AT]CC[CT]
Tsp45I        437  GTCAC                ^GT[GC]AC
Tth111I       868  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AluI          231  AGCT                 AG^CT
              294  AGCT                
BsmAI         607  GAGAC                GTCTC(1/5)
              954  GTCTC               
BsmFI          78  GGGAC                GGGAC(10/14)
              962  GGGAC               
BstF5I        318  GGATG                GGATG(2/0)
              408  GGATG               
BstNI         404  CCAGG                CC^[AT]GG
              657  CCTGG               
DraII         947  AGGCCCT              [GA]G^G[ACGT]CC[CT]
              962  GGGACCC             
DsaI          311  CCATGG               C^C[GA][CT]GG
              483  CCATGG              
EcoO109I      947  AGGCCCT              [GA]G^G[ACGT]CC[CT]
              962  GGGACCC             
EcoRII        404  CCAGG                ^CC[AT]GG
              657  CCTGG               
FauI          102  GCGGG                CCCGC(4/6)
              960  GCGGG               
FokI          318  GGATG                GGATG(9/13)
              408  GGATG               
HapII          60  CCGG                 C^CGG
              227  CCGG                
HpaII          60  CCGG                 C^CGG
              227  CCGG                
MaeIII        211  GTAAC                ^GT[ACGT]AC
              437  GTCAC               
MspI           60  CCGG                 C^CGG
              227  CCGG                
MvaI          404  CCAGG                CC^[AT]GG
              657  CCTGG               
NcoI          311  CCATGG               C^CATGG
              483  CCATGG              
PleI          832  GACTC                GAGTC(4/5)
              358  GAGTC               
SacI          230  GAGCTC               GAGCT^C
              293  GAGCTC              
SfaNI         379  GATGC                GCATC(5/9)
              449  GCATC               
TaqI          377  TCGA                 T^CGA
              992  TCGA                
TthHB8I       377  TCGA                 T^CGA
              992  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   46 enzymes) #
#########################################

AciI        AfaI        AlwI        AspHI       BanII       
BbvI        BmyI        BpmI        BsaJI       BseRI       
BsiHKAI     BsiYI       BslI        Bsp1286I    BsrI        
BssKI       BstYI       Cac8I       Cfr13I      DdeI        
DpnII       EcoT14I     Fnu4HI      HaeIII      HinfI       
ItaI        MboI        MboII       MflI        MnlI        
MseI        MwoI        NdeII       NlaIII      NlaIV       
RsaI        Sau3AI      Sau96I      ScrFI       StyI        
TfiI        Tru9I       TseI        Tsp509I     TspRI       
XhoII       
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