Restriction map for pF1KB5227
##################################
# NO CUT ENZYMES (  141 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      AocI        Aor51HI     ApaI        ApaLI       
AscI        Asp718I     AspEI       AspI        AvaI        
AviII       AvrII       BalI        BamHI       BanII       
BbeI        BbrPI       BbsI        BcgI        BclI        
BcnI        BfrI        BglI        BglII       BlnI        
BpuAI       BsaAI       BsaBI       BsaHI       BsaWI       
BseAI       BsiEI       BsiWI       BsmBI       BsoBI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrBI       BsrFI       BsrGI       BssHII      BssSI       
BstAPI      BstBI       BstEII      BstPI       BstUI       
BstXI       Bsu36I      Cfr10I      ClaI        CpoI        
DraIII      DrdI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoO65I     EcoRV       
EcoT22I     FauI        FbaI        FseI        FspI        
HapII       HgaI        Hin1I       HincII      HindII      
HindIII     HpaI        HpaII       KasI        KpnI        
KspI        MamI        MluI        MluNI       MroI        
MscI        MspI        MvnI        NaeI        NarI        
NciI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        Psp1406I    PvuI        RcaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       Sse8387I    SspBI       SspI        StuI        
SwaI        Tth111I     XbaI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AcsI          698  GAATTC               [GA]^AATT[CT]
ApoI          698  GAATTC               [GA]^AATT[CT]
AseI          486  ATTAAT               AT^TAAT
AsnI          486  ATTAAT               AT^TAAT
Asp700I       247  GAAGGTATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI         455  GAGCAC               G[AT]GC[AT]^C
BanI         1404  GGTGCC               G^G[CT][GA]CC
BbvCI         400  CCTCAGC              CCTCAGC(-5/-2)
BciVI        1357  GGATAC               GTATCC(6/5)
BlpI          146  GCTTAGC              GC^T[ACGT]AGC
Bpu1102I      146  GCTTAGC              GC^T[ACGT]AGC
BsgI          758  CTGCAC               GTGCAG(16/14)
BsiHKAI       455  GAGCAC               G[AT]GC[AT]^C
BsrDI         157  CATTGC               GCAATG(2/0)
Bst1107I      132  GTATAC               GTA^TAC
BstYI          71  AGATCC               [GA]^GATC[CT]
BstZ17I       132  GTATAC               GTA^TAC
CelII         146  GCTTAGC              GC^T[ACGT]AGC
CfoI          733  GCGC                 GCG^C
DraI          103  TTTAAA               TTT^AAA
DsaI           31  CCGTGG               C^C[GA][CT]GG
EarI         1165  GAAGAG               CTCTTC(1/4)
EcoRI         698  GAATTC               G^AATTC
EcoT14I       936  CCAAGG               C^C[AT][AT]GG
HaeII         732  AGCGCC               [GA]GCGC^[CT]
HhaI          733  GCGC                 GCG^C
HinP1I        733  GCGC                 G^CGC
HphI          589  GGTGA                GGTGA(8/7)
Ksp632I      1165  GAAGAG               CTCTTC(1/4)
MfeI         1250  CAATTG               C^AATTG
MflI           71  AGATCC               [GA]^GATC[CT]
MunI         1250  CAATTG               C^AATTG
PshAI         834  GACACCTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI         486  ATTAAT               AT^TAAT
ScaI         1060  AGTACT               AGT^ACT
SexAI         666  ACCAGGT              A^CC[AT]GGT
StyI          936  CCAAGG               C^C[AT][AT]GG
TaqI         1431  TCGA                 T^CGA
TfiI          334  GATTC                G^A[AT]TC
TspRI         816  CAGTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I      1431  TCGA                 T^CGA
XcmI          936  CCAAGGACCTGTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII          71  AGATCC               [GA]^GATC[CT]
XmnI          247  GAAGGTATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AccI          132  GTATAC               GT^[AC][GT]AC
             1029  GTCTAC              
AlwI           72  GATCC                GGATC(4/5)
             1137  GATCC               
AlwNI         137  CAGGACCTG            CAG[ACGT][ACGT][ACGT]^CTG
              767  CAGCAACTG           
BpmI          470  CTCCAG               CTGGAG(16/14)
              791  CTCCAG              
BsaI         1302  GAGACC               GGTCTC(1/5)
             1449  GAGACC              
BseRI         503  CTCCTC               GAGGAG(10/8)
             1427  CTCCTC              
BsmAI        1302  GAGAC                GTCTC(1/5)
             1449  GAGAC               
BsmFI         832  GGGAC                GGGAC(10/14)
             1392  GGGAC               
DpnII          72  GATC                 ^GATC
             1137  GATC                
EaeI         1210  CGGCCA               [CT]^GGCC[GA]
             1473  TGGCCG              
MaeII         690  ACGT                 A^CGT
             1197  ACGT                
MboI           72  GATC                 ^GATC
             1137  GATC                
MslI          597  CATAATGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1141  CATCCAGATG          
NdeII          72  GATC                 ^GATC
             1137  GATC                
PleI          358  GACTC                GAGTC(4/5)
              711  GACTC               
PpuMI         138  AGGACCT              [GA]G^G[AT]CC[CT]
              939  AGGACCT             
PstI          362  CTGCAG               CTGCA^G
             1064  CTGCAG              
PvuII          22  CAGCTG               CAG^CTG
              929  CAGCTG              
Sau3AI         72  GATC                 ^GATC
             1137  GATC                
SmlI          713  CTCAAG               C^T[CT][GA]AG
              849  CTCAAG              
SpeI         1045  ACTAGT               A^CTAGT
             1117  ACTAGT              
Tsp45I        119  GTCAC                ^GT[GC]AC
              841  GTCAC               


#########################################
# 3 OR MORE CUT ENZYMES (   51 enzymes) #
#########################################

AciI        AfaI        AluI        AvaII       BbvI        
BfaI        BmyI        BsaJI       BsiYI       BslI        
BsmI        Bsp1286I    BspMI       BsrI        BssKI       
BstF5I      BstNI       Cac8I       Cfr13I      DdeI        
DraII       EcoNI       EcoO109I    EcoRII      Fnu4HI      
FokI        HaeIII      HinfI       ItaI        MaeI        
MaeIII      MboII       MnlI        MseI        MspA1I      
MvaI        MwoI        NlaIII      NlaIV       NspI        
PflMI       RsaI        Sau96I      ScrFI       SfaNI       
SfcI        SphI        Tru9I       TseI        Tsp509I     
Van91I      
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