Restriction map for pF1KB5212
##################################
# NO CUT ENZYMES (  122 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflII       
AgeI        AhdI        Alw44I      Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp718I     
AspEI       AspHI       AvrII       BbeI        BbrPI       
BbsI        BbvCI       BciVI       BfaI        BfrI        
BglI        BlnI        BlpI        Bpu1102I    BpuAI       
BsaAI       BsaWI       BseAI       BsiEI       BsiHKAI     
BsiWI       BsmBI       BsmFI       BsmI        Bsp1407I    
BspDI       BspEI       BspHI       BsrBI       BsrDI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstBI       BstEII      BstPI       BstXI       BstZ17I     
CelII       Cfr10I      ClaI        CpoI        DraI        
DraIII      EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoO65I     EcoRV       EcoT22I     FseI        
HaeII       HindIII     HpaI        KasI        KpnI        
KspI        MaeI        MfeI        MroI        MunI        
NaeI        NarI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacI        
SacII       SalI        SapI        SfiI        SfoI        
SfuI        SgrAI       SmlI        SnaBI       SpeI        
SspBI       SspI        StuI        SwaI        Van91I      
XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AccI          106  GTCTAC               GT^[AC][GT]AC
AcyI         1064  GGCGTC               G[GA]^CG[CT]C
AlwNI         583  CAGGACCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          961  CCTGAGG              CC^T[ACGT]AGG
AspI          643  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII         845  TGCGCA               TGC^GCA
BanI          339  GGTGCC               G^G[CT][GA]CC
BanII         979  GAGCCC               G[GA]GC[CT]^C
BcgI          717  CGATGTGACTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI          551  TGATCA               T^GATCA
BglII         882  AGATCT               A^GATCT
BpmI          143  CTGGAG               CTGGAG(16/14)
BsaBI         177  GATGAGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI        1064  GGCGTC               G[GA]^CG[CT]C
BsaI          625  GAGACC               GGTCTC(1/5)
BseRI          43  GAGGAG               GAGGAG(10/8)
BsgI          274  GTGCAG               GTGCAG(16/14)
BsmAI         625  GAGAC                GTCTC(1/5)
BspLU11I      659  ACATGT               A^CATGT
Bsu36I        961  CCTGAGG              CC^T[ACGT]AGG
DrdI           97  GACAAGCAGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI          347  CCGTGG               C^C[GA][CT]GG
Eco81I        961  CCTGAGG              CC^T[ACGT]AGG
EcoRI         433  GAATTC               G^AATTC
EcoT14I       449  CCAAGG               C^C[AT][AT]GG
FauI          228  CCCGC                CCCGC(4/6)
FbaI          551  TGATCA               T^GATCA
FspI          845  TGCGCA               TGC^GCA
HgaI         1065  GCGTC                GACGC(5/10)
Hin1I        1064  GGCGTC               G[GA]^CG[CT]C
HincII        649  GTCAAC               GT[CT]^[GA]AC
HindII        649  GTCAAC               GT[CT]^[GA]AC
MamI          177  GATGAGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI          996  ACGCGT               A^CGCGT
PflFI         643  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
SbfI          795  CCTGCAGG             CCTGCA^GG
SexAI         335  ACCTGGT              A^CC[AT]GGT
SmaI         1004  CCCGGG               CCC^GGG
Sse8387I      795  CCTGCAGG             CCTGCA^GG
StyI          449  CCAAGG               C^C[AT][AT]GG
Tth111I       643  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI        1004  CCCGGG               C^CCGGG
XmaI         1004  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AccII         682  CGCG                 CG^CG
              997  CGCG                
AcsI          433  GAATTC               [GA]^AATT[CT]
              940  GAATTT              
AflIII        659  ACATGT               A^C[GA][CT]GT
              996  ACGCGT              
ApoI          433  GAATTC               [GA]^AATT[CT]
              940  GAATTT              
Asp700I       369  GAACCAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              810  GAACCTCTTC          
BamHI         182  GGATCC               G^GATCC
              701  GGATCC              
BstAPI        120  GCACCGCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              879  GCAAGATCTGC         
BstUI         682  CGCG                 CG^CG
              997  CGCG                
EarI          814  CTCTTC               CTCTTC(1/4)
              220  GAAGAG              
EcoNI         294  CCTGAAAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              795  CCTGCAGGAGG         
HinfI         148  GAATC                G^A[ACGT]TC
              617  GAATC               
HphI          256  GGTGA                GGTGA(8/7)
             1088  TCACC               
Ksp632I       814  CTCTTC               CTCTTC(1/4)
              220  GAAGAG              
MseI          194  TTAA                 T^TAA
              203  TTAA                
MspA1I         85  CAGCTG               C[AC]G^C[GT]G
              508  CAGCTG              
MvnI          682  CGCG                 CG^CG
              997  CGCG                
PstI           88  CTGCAG               CTGCA^G
              796  CTGCAG              
PvuII          85  CAGCTG               CAG^CTG
              508  CAGCTG              
ScaI          527  AGTACT               AGT^ACT
             1013  AGTACT              
SfaNI         967  GATGC                GCATC(5/9)
              824  GCATC               
SfcI           88  CTGCAG               C^T[GA][CT]AG
              796  CTGCAG              
SphI          444  GCATGC               GCATG^C
             1154  GCATGC              
TfiI          148  GAATC                G^A[AT]TC
              617  GAATC               
Tru9I         194  TTAA                 T^TAA
              203  TTAA                
Tsp509I       434  AATT                 ^AATT
              941  AATT                
XmnI          369  GAACCAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              810  GAACCTCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   67 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AvaI        
AvaII       BalI        BbvI        BcnI        BmyI        
BsaJI       BsiYI       BslI        BsoBI       Bsp1286I    
BspMI       BsrI        BssKI       BstF5I      BstNI       
BstYI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraII       EaeI        EcoO109I    EcoRII      
Fnu4HI      FokI        HaeIII      HapII       HhaI        
HinP1I      HpaII       ItaI        MaeII       MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MscI        MslI        MspI        MvaI        MwoI        
NciI        NdeII       NlaIII      NlaIV       NspI        
PpuMI       RsaI        Sau3AI      Sau96I      ScrFI       
TaqI        TseI        Tsp45I      TspRI       TthHB8I     
XcmI        XhoII       
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