Restriction map for pF1KB5205
##################################
# NO CUT ENZYMES (  121 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcsI        AflII       AgeI        AhdI        Alw44I      
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp718I     AspEI       AspI        
AviII       AvrII       BamHI       BbrPI       BciVI       
BclI        BfaI        BfrI        BglII       BlnI        
BlpI        Bpu1102I    BsaAI       BsaWI       BseAI       
BsgI        BsiEI       BsiWI       BsmBI       Bsp1407I    
BspDI       BspEI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstUI       BstZ17I     CelII       Cfr10I      
ClaI        CpoI        DraI        DraIII      EagI        
Eam1105I    EclXI       Eco47III    Eco52I      EcoNI       
EcoO65I     EcoRI       EcoRV       EcoT22I     FbaI        
FseI        FspI        HgaI        KpnI        KspI        
MaeI        MaeII       MluI        MroI        MvnI        
NaeI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
RsrII       SacII       SalI        SapI        SbfI        
SexAI       SfiI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        Sse8387I    SspBI       SspI        
SwaI        Tth111I     Van91I      XbaI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AcyI         1129  GGCGCC               G[GA]^CG[CT]C
AflIII        920  ACATGT               A^C[GA][CT]GT
AocI          943  CCTGAGG              CC^T[ACGT]AGG
BalI          893  TGGCCA               TGG^CCA
BbeI         1129  GGCGCC               GGCGC^C
BbsI          651  GAAGAC               GAAGAC(2/6)
BcgI         1443  CGAGGACATTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI         1602  CCGGG                CC^[GC]GG
BglI         1516  GCCTCTGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI          851  CTGGAG               CTGGAG(16/14)
BpuAI         651  GAAGAC               GAAGAC(2/6)
BsaBI         582  GATTGGAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI        1129  GGCGCC               G[GA]^CG[CT]C
BsaI         1048  GAGACC               GGTCTC(1/5)
BspHI        1178  TCATGA               T^CATGA
BspLU11I      920  ACATGT               A^CATGT
Bsu36I        943  CCTGAGG              CC^T[ACGT]AGG
DraII         120  GGGTCCC              [GA]G^G[ACGT]CC[CT]
DrdI         1152  GACAGAGCGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I        943  CCTGAGG              CC^T[ACGT]AGG
EcoO109I      120  GGGTCCC              [GA]G^G[ACGT]CC[CT]
HaeII        1129  GGCGCC               [GA]GCGC^[CT]
HapII        1602  CCGG                 C^CGG
Hin1I        1129  GGCGCC               G[GA]^CG[CT]C
HincII        397  GTTAAC               GT[CT]^[GA]AC
HindII        397  GTTAAC               GT[CT]^[GA]AC
HpaI          397  GTTAAC               GTT^AAC
HpaII        1602  CCGG                 C^CGG
KasI         1129  GGCGCC               G^GCGCC
MamI          582  GATTGGAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI          794  CAATTG               C^AATTG
MluNI         893  TGGCCA               TGG^CCA
MscI          893  TGGCCA               TGG^CCA
MspI         1602  CCGG                 C^CGG
MunI          794  CAATTG               C^AATTG
NarI         1129  GGCGCC               GG^CGCC
NciI         1602  CCGGG                CC^[GC]GG
PleI          767  GAGTC                GAGTC(4/5)
PpuMI         120  GGGTCCC              [GA]G^G[AT]CC[CT]
RcaI         1178  TCATGA               T^CATGA
ScaI          902  AGTACT               AGT^ACT
SfoI         1129  GGCGCC               GGC^GCC
SphI         1401  GCATGC               GCATG^C
StuI         1319  AGGCCT               AGG^CCT
XcmI          881  CCATTGGGGATGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   31 enzymes) #
###################################

AccI          238  GTAGAC               GT^[AC][GT]AC
             1429  GTAGAC              
Asp700I       513  GAAAGCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              651  GAAGACTTTC          
AspHI         489  GAGCTC               G[AT]GC[AT]^C
              727  GAGCTC              
AvaI          613  CTCGAG               C^[CT]CG[GA]G
              730  CTCGAG              
AvaII         121  GGTCC                G^G[AT]CC
             1160  GGTCC               
BanI          277  GGCACC               G^G[CT][GA]CC
             1129  GGCGCC              
BbvCI          97  GCTGAGG              CCTCAGC(-5/-2)
              262  GCTGAGG             
BseRI         946  GAGGAG               GAGGAG(10/8)
             1147  GAGGAG              
BsiHKAI       489  GAGCTC               G[AT]GC[AT]^C
              727  GAGCTC              
BsiYI         126  CCGTGGCATGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              879  CCCCATTGGGG         
BslI          126  CCGTGGCATGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              879  CCCCATTGGGG         
BsmAI        1048  GAGAC                GTCTC(1/5)
             1150  GAGAC               
BsmI         1002  GAATGC               GAATGC(1/-1)
              643  GCATTC              
BsoBI         613  CTCGAG               C^[CT]CG[GA]G
              730  CTCGAG              
BspMI        1256  ACCTGC               ACCTGC(4/8)
              318  GCAGGT              
BsrDI         243  CATTGC               GCAATG(2/0)
             1449  CATTGC              
BstYI         839  AGATCC               [GA]^GATC[CT]
              951  GGATCT              
CfoI         1130  GCGC                 GCG^C
             1504  GCGC                
EarI          957  GAAGAG               CTCTTC(1/4)
             1045  GAAGAG              
HhaI         1130  GCGC                 GCG^C
             1504  GCGC                
HinP1I       1130  GCGC                 G^CGC
             1504  GCGC                
Ksp632I       957  GAAGAG               CTCTTC(1/4)
             1045  GAAGAG              
MflI          839  AGATCC               [GA]^GATC[CT]
              951  GGATCT              
MseI          398  TTAA                 T^TAA
              575  TTAA                
MslI          282  CACCTCAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1233  CATTGAGATG          
PaeR7I        613  CTCGAG               C^TCGAG
              730  CTCGAG              
SacI          489  GAGCTC               GAGCT^C
              727  GAGCTC              
Tru9I         398  TTAA                 T^TAA
              575  TTAA                
XhoI          613  CTCGAG               C^TCGAG
              730  CTCGAG              
XhoII         839  AGATCC               [GA]^GATC[CT]
              951  GGATCT              
XmnI          513  GAAAGCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              651  GAAGACTTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   61 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AlwNI       
BanII       BbvI        BmyI        BsaJI       BsmFI       
Bsp1286I    BsrBI       BsrI        BssKI       BstF5I      
BstNI       BstXI       Cac8I       Cfr13I      DdeI        
DpnII       DsaI        EaeI        EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeIII      HindIII     
HinfI       HphI        ItaI        MaeIII      MboI        
MboII       MnlI        MspA1I      MvaI        MwoI        
NdeII       NlaIII      NlaIV       NspI        PstI        
PvuII       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        StyI        TaqI        
TfiI        TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     
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