Restriction map for pF1KB5184
##################################
# NO CUT ENZYMES (  130 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AocI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AvrII       BalI        BbeI        
BbrPI       BbsI        BfrI        BglII       BlnI        
BlpI        Bpu1102I    BpuAI       BsaAI       BsaHI       
BsaWI       BseAI       BseRI       BsiEI       BsiWI       
BsmBI       BspDI       BspEI       BspLU11I    BsrBI       
BsrDI       BsrFI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstUI       
BstZ17I     Bsu36I      CelII       Cfr10I      ClaI        
CpoI        DraI        DraIII      DrdI        DsaI        
EagI        Eam1105I    EclXI       Eco52I      Eco81I      
EcoNI       EcoO65I     EcoRI       EcoT22I     FseI        
HgaI        Hin1I       HpaI        KasI        KpnI        
KspI        MluI        MluNI       MroI        MscI        
MseI        MvnI        NaeI        NarI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        RsrII       SacII       
SalI        SbfI        SexAI       SfoI        SfuI        
SgrAI       SmaI        SnaBI       SphI        Sse8387I    
SspI        SwaI        Tru9I       Tth111I     XbaI        
XcmI        XhoI        XmaCI       XmaI        XmnI        


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# ONE CUT ENZYMES (   34 enzymes) #
###################################

AccI          252  GTCTAC               GT^[AC][GT]AC
Aor51HI       902  AGCGCT               AGC^GCT
AspHI        1499  GAGCTC               G[AT]GC[AT]^C
AviII        1184  TGCGCA               TGC^GCA
BbvCI        1459  GCTGAGG              CCTCAGC(-5/-2)
BclI          573  TGATCA               T^GATCA
BsaBI         891  GATTCCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI      1499  GAGCTC               G[AT]GC[AT]^C
Bsp1407I     1034  TGTACA               T^GTACA
BspHI        1139  TCATGA               T^CATGA
BsrGI        1034  TGTACA               T^GTACA
BstXI        1623  CCAAGAGAGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EaeI          603  TGGCCG               [CT]^GGCC[GA]
Eco47III      902  AGCGCT               AGC^GCT
EcoRV        1564  GATATC               GAT^ATC
FbaI          573  TGATCA               T^GATCA
FspI         1184  TGCGCA               TGC^GCA
HaeII         902  AGCGCT               [GA]GCGC^[CT]
HincII         90  GTTGAC               GT[CT]^[GA]AC
HindII         90  GTTGAC               GT[CT]^[GA]AC
HindIII        50  AAGCTT               A^AGCTT
MaeII        1232  ACGT                 A^CGT
MamI          891  GATTCCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         1003  CAATTG               C^AATTG
MunI         1003  CAATTG               C^AATTG
NspI          911  ACATGC               [GA]CATG^[CT]
RcaI         1139  TCATGA               T^CATGA
SacI         1499  GAGCTC               GAGCT^C
SapI         1746  GCTCTTC              GCTCTTC(1/4)
ScaI         1654  AGTACT               AGT^ACT
SfiI          630  GGCCTCAATGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI         1369  CTCAAG               C^T[CT][GA]AG
SpeI         1657  ACTAGT               A^CTAGT
SspBI        1034  TGTACA               T^GTACA


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AlwNI          82  CAGACTCTG            CAG[ACGT][ACGT][ACGT]^CTG
              277  CAGGTTCTG           
AvaI          224  CTCGGG               C^[CT]CG[GA]G
              818  CTCGGG              
BamHI        1398  GGATCC               G^GATCC
             1589  GGATCC              
BcgI           15  CGAGAGTGGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             1511  GCATTGGGCTCG        
BciVI         498  GGATAC               GTATCC(6/5)
             1208  GGATAC              
BpmI          784  CTCCAG               CTGGAG(16/14)
              145  CTGGAG              
BsmI          327  GAATGC               GAATGC(1/-1)
             1530  GAATGC              
BsoBI         224  CTCGGG               C^[CT]CG[GA]G
              818  CTCGGG              
BspMI         234  ACCTGC               ACCTGC(4/8)
               25  GCAGGT              
BstYI        1398  GGATCC               [GA]^GATC[CT]
             1589  GGATCC              
EarI         1747  CTCTTC               CTCTTC(1/4)
              556  GAAGAG              
Ksp632I      1747  CTCTTC               CTCTTC(1/4)
              556  GAAGAG              
MaeIII         97  GTGAC                ^GT[ACGT]AC
              346  GTGAC               
MflI         1398  GGATCC               [GA]^GATC[CT]
             1589  GGATCC              
PpuMI         113  AGGTCCC              [GA]G^G[AT]CC[CT]
              657  GGGTCCC             
PstI         1454  CTGCAG               CTGCA^G
             1466  CTGCAG              
SfcI         1454  CTGCAG               C^T[GA][CT]AG
             1466  CTGCAG              
StuI          131  AGGCCT               AGG^CCT
              245  AGGCCT              
TaqI           73  TCGA                 T^CGA
             1503  TCGA                
Tsp45I         97  GTGAC                ^GT[GC]AC
              346  GTGAC               
Tsp509I      1004  AATT                 ^AATT
             1014  AATT                
TthHB8I        73  TCGA                 T^CGA
             1503  TCGA                
XhoII        1398  GGATCC               [GA]^GATC[CT]
             1589  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   71 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        ApaI        
AvaII       BanI        BanII       BbvI        BcnI        
BfaI        BglI        BmyI        BsaI        BsaJI       
BsgI        BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    BsrI        BssKI       BstF5I      BstNI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeIII      HapII       HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
MaeI        MboI        MboII       MnlI        MslI        
MspA1I      MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PflMI       PleI        
PvuII       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       StyI        TfiI        TseI        TspRI       
Van91I      
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