Restriction map for pF1KB5184
 (Linear) (Six-base) MAPSORT of: KIBB5184.seq  Check: 1980  from: 1  to: 1818



KIBB5184.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:27  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     243    1818 
   Size:        243    1575
 
AccI GT'mk_AC

Cuts at:      0     253    1818 
   Size:        253    1565
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      38      68     824     937    1493    1689    1818 
   Size:         38      30     756     113     556     196     129

  Fragments arranged by size: 

                 756     556     196     129     113      38      30
 
AfeI AGC'GCT

Cuts at:      0     904    1818 
   Size:        904     914
 
AleI CACnn'nnGTG

Cuts at:      0     943    1818 
   Size:        943     875
 
AlwNI CAG_nnn'CTG

Cuts at:      0      87     282    1818 
   Size:         87     195    1536

  Fragments arranged by size: 

                1536     195      87
 
ApaI G_GGCC'C

Cuts at:      0     678     714    1544    1818 
   Size:        678      36     830     274

  Fragments arranged by size: 

                 830     678     274      36
 
AvaI C'yCGr_G

Cuts at:      0     224     818    1818 
   Size:        224     594    1000

  Fragments arranged by size: 

                1000     594     224
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     263     296     383     416    1818 
   Size:        263      33      87      33    1402

  Fragments arranged by size: 

                1402     263      87      33      33
 
BamHI G'GATC_C

Cuts at:      0    1398    1589    1818 
   Size:       1398     191     229

  Fragments arranged by size: 

                1398     229     191
 
BanI G'GyrC_C

Cuts at:      0     231    1648    1714    1771    1795    1818 
   Size:        231    1417      66      57      24      23

  Fragments arranged by size: 

                1417     231      66      57      24      23
 
BanII G_rGCy'C

Cuts at:      0     678     714    1061    1503    1520    1544    1748    1818 
   Size:        678      36     347     442      17      24     204      70

  Fragments arranged by size: 

                 678     442     347     204      70      36      24      17
            
 
BbvCI CC'TCA_GC

Cuts at:      0    1460    1818 
   Size:       1460     358
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0       4      38    1500    1534    1818 
   Size:          4      34    1462      34     284

  Fragments arranged by size: 

                1462     284      34      34       4
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     492    1202    1818 
   Size:        492     710     616

  Fragments arranged by size: 

                 710     616     492
 
BclI T'GATC_A

Cuts at:      0     573    1818 
   Size:        573    1245
 
BglI GCCn_nnn'nGGC

Cuts at:      0     139     244     637     646     673     682    1818 
   Size:        139     105     393       9      27       9    1136

  Fragments arranged by size: 

                1136     393     139     105      27       9       9
 
Bme1580I G_kGCm'C

Cuts at:      0     678     714    1544    1818 
   Size:        678      36     830     274

  Fragments arranged by size: 

                 830     678     274      36
 
BmgBI CAC'GTC

Cuts at:      0    1233    1818 
   Size:       1233     585
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     474    1087    1560    1818 
   Size:        474     613     473     258

  Fragments arranged by size: 

                 613     474     473     258
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     742    1818 
   Size:        742    1076
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     166     769    1818 
   Size:        166     603    1049

  Fragments arranged by size: 

                1049     603     166
 
Bpu10I CC'TnA_GC

Cuts at:      0     413     458    1460    1818 
   Size:        413      45    1002     358

  Fragments arranged by size: 

                1002     413     358      45
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1354    1818 
   Size:       1354     464
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     156     174     192     210     358     618    1342    1818 
   Size:        156      18      18      18     148     260     724     476

  Fragments arranged by size: 

                 724     476     260     156     148      18      18      18
            
 
BsaBI GATnn'nnATC

Cuts at:      0     895    1818 
   Size:        895     923
 
BseYI C'CCAG_C

Cuts at:      0     444    1104    1818 
   Size:        444     660     714

  Fragments arranged by size: 

                 714     660     444
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      44     413     491    1818 
   Size:         44     369      78    1327

  Fragments arranged by size: 

                1327     369      78      44
 
BsiHKAI G_wGCw'C

Cuts at:      0    1503    1818 
   Size:       1503     315
 
BsmI GAATG_Cn'

Cuts at:      0     333    1536    1818 
   Size:        333    1203     282

  Fragments arranged by size: 

                1203     333     282
 
Bsp1286I G_dGCh'C

Cuts at:      0     678     714    1061    1503    1520    1544    1748    1818 
   Size:        678      36     347     442      17      24     204      70

  Fragments arranged by size: 

                 678     442     347     204      70      36      24      17
            
 
BspHI T'CATG_A

Cuts at:      0    1139    1818 
   Size:       1139     679
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      16     243    1818 
   Size:         16     227    1575

  Fragments arranged by size: 

                1575     227      16
 
BsrGI T'GTAC_A

Cuts at:      0    1034    1818 
   Size:       1034     784
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1630    1818 
   Size:       1630     188
 
BstYI r'GATC_y

Cuts at:      0    1398    1589    1818 
   Size:       1398     191     229

  Fragments arranged by size: 

                1398     229     191
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1447    1818 
   Size:       1447     371
 
BtsI GCAGTG_nn'

Cuts at:      0     576    1641    1818 
   Size:        576    1065     177

  Fragments arranged by size: 

                1065     576     177
 
EaeI y'GGCC_r

Cuts at:      0     603    1818 
   Size:        603    1215
 
EarI CTCTTCn'nnn_

Cuts at:      0     551    1753    1818 
   Size:        551    1202      65

  Fragments arranged by size: 

                1202     551      65
 
EcoICRI GAG'CTC

Cuts at:      0    1501    1818 
   Size:       1501     317
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      38      68     166     769     824     937    1493    1689 
   Size:         38      30      98     603      55     113     556     196

Cuts at:   1689    1818 
   Size:        129

  Fragments arranged by size: 

                 603     556     196     129     113      98      55      38
                  30
 
EcoO109I rG'GnC_Cy

Cuts at:      0     114     441     658    1329    1541    1818 
   Size:        114     327     217     671     212     277

  Fragments arranged by size: 

                 671     327     277     217     212     114
 
EcoRV GAT'ATC

Cuts at:      0    1566    1818 
   Size:       1566     252
 
FspI TGC'GCA

Cuts at:      0    1186    1818 
   Size:       1186     632
 
HaeII r_GCGC'y

Cuts at:      0     906    1818 
   Size:        906     912
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      55      69      87     101    1485    1517    1716    1748 
   Size:         55      14      18      14    1384      32     199      32

Cuts at:   1748    1818 
   Size:         70

  Fragments arranged by size: 

                1384     199      70      55      32      32      18      14
                  14
 
HincII GTy'rAC

Cuts at:      0      92    1818 
   Size:         92    1726
 
HindIII A'AGCT_T

Cuts at:      0      50    1818 
   Size:         50    1768
 
MfeI C'AATT_G

Cuts at:      0    1003    1818 
   Size:       1003     815
 
MslI CAynn'nnrTG

Cuts at:      0     548     943    1138    1818 
   Size:        548     395     195     680

  Fragments arranged by size: 

                 680     548     395     195
 
MspA1I CmG'CkG

Cuts at:      0     264     289     310     475    1459    1471    1818 
   Size:        264      25      21     165     984      12     347

  Fragments arranged by size: 

                 984     347     264     165      25      21      12
 
NspI r_CATG'y

Cuts at:      0     915    1818 
   Size:        915     903
 
PasI CC'CwG_GG

Cuts at:      0    1204    1574    1818 
   Size:       1204     370     244

  Fragments arranged by size: 

                1204     370     244
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     108     282    1409    1818 
   Size:        108     174    1127     409

  Fragments arranged by size: 

                1127     409     174     108
 
PpuMI rG'GwC_Cy

Cuts at:      0     114     658    1818 
   Size:        114     544    1160

  Fragments arranged by size: 

                1160     544     114
 
PspOMI G'GGCC_C

Cuts at:      0     674     710    1540    1818 
   Size:        674      36     830     278

  Fragments arranged by size: 

                 830     674     278      36
 
PstI C_TGCA'G

Cuts at:      0    1458    1470    1818 
   Size:       1458      12     348

  Fragments arranged by size: 

                1458     348      12
 
PvuII CAG'CTG

Cuts at:      0     264     289     310     475    1459    1471    1818 
   Size:        264      25      21     165     984      12     347

  Fragments arranged by size: 

                 984     347     264     165      25      21      12
 
SacI G_AGCT'C

Cuts at:      0    1503    1818 
   Size:       1503     315
 
SanDI GG'GwC_CC

Cuts at:      0     658    1818 
   Size:        658    1160
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1753    1818 
   Size:       1753      65
 
ScaI AGT'ACT

Cuts at:      0    1656    1818 
   Size:       1656     162
 
SfcI C'TryA_G

Cuts at:      0    1454    1466    1818 
   Size:       1454      12     352

  Fragments arranged by size: 

                1454     352      12
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     637    1818 
   Size:        637    1181
 
SmlI C'TyrA_G

Cuts at:      0    1369    1818 
   Size:       1369     449
 
SpeI A'CTAG_T

Cuts at:      0    1657    1818 
   Size:       1657     161
 
StuI AGG'CCT

Cuts at:      0     133     247    1818 
   Size:        133     114    1571

  Fragments arranged by size: 

                1571     133     114
 
StyI C'CwwG_G

Cuts at:      0     509    1403    1478    1758    1818 
   Size:        509     894      75     280      60

  Fragments arranged by size: 

                 894     509     280      75      60
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     256    1113    1142    1818 
   Size:        256     857      29     676

  Fragments arranged by size: 

                 857     676     256      29
 
TatI w'GTAC_w

Cuts at:      0    1034    1654    1818 
   Size:       1034     620     164

  Fragments arranged by size: 

                1034     620     164
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AfeI     AleI    AlwNI     ApaI     AvaI
     BaeI    BamHI     BanI    BanII    BbvCI     BcgI    BciVI     BclI
     BglI Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaBI    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I    BspHI
    BspMI    BsrGI    BstXI    BstYI    BtgZI     BtsI     EaeI     EarI
  EcoICRI  Eco57MI EcoO109I    EcoRV     FspI    HaeII    Hin4I   HincII
  HindIII     MfeI     MslI   MspA1I     NspI     PasI    PflMI    PpuMI
   PspOMI     PstI    PvuII     SacI    SanDI     SapI     ScaI     SfcI
     SfiI     SmlI     SpeI     StuI     StyI    TaqII     TatI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI    AflII   AflIII     AgeI     AhdI     AlfI
     AloI    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII     BbeI
     BbsI    BfrBI    BglII     BlpI     BmtI    BsaAI    BsaHI    BsaWI
    BsaXI    BseRI    BsiEI    BsiWI    BsmBI    BspEI    BsrBI    BsrDI
    BsrFI   BssHII    BssSI   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I
     BtgI     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EciI
    EcoNI    EcoRI     FalI     FseI    FspAI     HpaI     KasI     KpnI
     MluI     MmeI     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PciI     PfoI     PmeI
     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII
    SacII     SalI     SbfI    SexAI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SphI     SrfI     SspI     SwaI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI
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