Restriction map for pF1KB5154
 (Linear) (Six-base) MAPSORT of: KIBB5154.seq  Check: 6865  from: 1  to: 1884



KIBB5154.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:27  ..

AatII G_ACGT'C

Cuts at:      0    1454    1829    1884 
   Size:       1454     375      55

  Fragments arranged by size: 

                1454     375      55
 
AccI GT'mk_AC

Cuts at:      0     408     832    1884 
   Size:        408     424    1052

  Fragments arranged by size: 

                1052     424     408
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     200    1499    1618    1884 
   Size:        200    1299     119     266

  Fragments arranged by size: 

                1299     266     200     119
 
AlwNI CAG_nnn'CTG

Cuts at:      0      22    1124    1884 
   Size:         22    1102     760

  Fragments arranged by size: 

                1102     760      22
 
ApoI r'AATT_y

Cuts at:      0     757     843    1363    1537    1884 
   Size:        757      86     520     174     347

  Fragments arranged by size: 

                 757     520     347     174      86
 
AseI AT'TA_AT

Cuts at:      0    1469    1884 
   Size:       1469     415
 
AvaI C'yCGr_G

Cuts at:      0    1063    1884 
   Size:       1063     821
 
AvrII C'CTAG_G

Cuts at:      0    1329    1884 
   Size:       1329     555
 
BanI G'GyrC_C

Cuts at:      0      37    1255    1798    1884 
   Size:         37    1218     543      86

  Fragments arranged by size: 

                1218     543      86      37
 
BanII G_rGCy'C

Cuts at:      0     164     301    1167    1884 
   Size:        164     137     866     717

  Fragments arranged by size: 

                 866     717     164     137
 
BbeI G_GCGC'C

Cuts at:      0    1802    1884 
   Size:       1802      82
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     568    1487    1884 
   Size:        568     919     397

  Fragments arranged by size: 

                 919     568     397
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     261     279    1760    1884 
   Size:        261      18    1481     124

  Fragments arranged by size: 

                1481     261     124      18
 
BglII A'GATC_T

Cuts at:      0    1178    1884 
   Size:       1178     706
 
BlpI GC'TnA_GC

Cuts at:      0     965    1037    1884 
   Size:        965      72     847

  Fragments arranged by size: 

                 965     847      72
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     158     621    1164    1321    1884 
   Size:        158     463     543     157     563

  Fragments arranged by size: 

                 563     543     463     158     157
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     156    1253    1884 
   Size:        156    1097     631

  Fragments arranged by size: 

                1097     631     156
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     149    1127    1884 
   Size:        149     978     757

  Fragments arranged by size: 

                 978     757     149
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     630    1103    1884 
   Size:        630     473     781

  Fragments arranged by size: 

                 781     630     473
 
BsaAI yAC'GTr

Cuts at:      0    1839    1884 
   Size:       1839      45
 
BsaBI GATnn'nnATC

Cuts at:      0     554    1019    1884 
   Size:        554     465     865

  Fragments arranged by size: 

                 865     554     465
 
BsaHI Gr'CG_yC

Cuts at:      0    1451    1799    1826    1884 
   Size:       1451     348      27      58

  Fragments arranged by size: 

                1451     348      58      27
 
BsaWI w'CCGG_w

Cuts at:      0     476    1884 
   Size:        476    1408
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1851    1881    1884 
   Size:       1851      30       3

  Fragments arranged by size: 

                1851      30       3
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     113     902    1884 
   Size:        113     789     982

  Fragments arranged by size: 

                 982     789     113
 
BseYI C'CCAG_C

Cuts at:      0     262    1085    1322    1689    1793    1884 
   Size:        262     823     237     367     104      91

  Fragments arranged by size: 

                 823     367     262     237     104      91
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1698    1884 
   Size:       1698     186
 
BsiEI CG_ry'CG

Cuts at:      0      62    1884 
   Size:         62    1822
 
BsiHKAI G_wGCw'C

Cuts at:      0    1167    1814    1884 
   Size:       1167     647      70

  Fragments arranged by size: 

                1167     647      70
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1442    1884 
   Size:       1442     442
 
Bsp1286I G_dGCh'C

Cuts at:      0     164     301    1167    1814    1884 
   Size:        164     137     866     647      70

  Fragments arranged by size: 

                 866     647     164     137      70
 
BspEI T'CCGG_A

Cuts at:      0     476    1884 
   Size:        476    1408
 
BspHI T'CATG_A

Cuts at:      0     416    1884 
   Size:        416    1468
 
BsrBI CCG'CTC

Cuts at:      0      58     121    1884 
   Size:         58      63    1763

  Fragments arranged by size: 

                1763      63      58
 
BsrDI GCAATG_nn'

Cuts at:      0     352     435    1501    1884 
   Size:        352      83    1066     383

  Fragments arranged by size: 

                1066     383     352      83
 
BsrFI r'CCGG_y

Cuts at:      0      99    1190    1884 
   Size:         99    1091     694

  Fragments arranged by size: 

                1091     694      99
 
BsrGI T'GTAC_A

Cuts at:      0     358    1703    1884 
   Size:        358    1345     181

  Fragments arranged by size: 

                1345     358     181
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     446    1884 
   Size:        446    1438
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     457    1414    1884 
   Size:        457     957     470

  Fragments arranged by size: 

                 957     470     457
 
BstYI r'GATC_y

Cuts at:      0     137     720     735    1178    1761    1884 
   Size:        137     583      15     443     583     123

  Fragments arranged by size: 

                 583     583     443     137     123      15
 
BtgI C'CryG_G

Cuts at:      0     302    1802    1873    1884 
   Size:        302    1500      71      11

  Fragments arranged by size: 

                1500     302      71      11
 
BtsI GCAGTG_nn'

Cuts at:      0     828    1884 
   Size:        828    1056
 
DraI TTT'AAA

Cuts at:      0     848    1884 
   Size:        848    1036
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     534    1497    1884 
   Size:        534     963     387

  Fragments arranged by size: 

                 963     534     387
 
EaeI y'GGCC_r

Cuts at:      0      24     318     518     655    1884 
   Size:         24     294     200     137    1229

  Fragments arranged by size: 

                1229     294     200     137      24
 
EarI CTCTTCn'nnn_

Cuts at:      0      77     305    1434    1585    1884 
   Size:         77     228    1129     151     299

  Fragments arranged by size: 

                1129     299     228     151      77
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1395    1884 
   Size:       1395     489
 
EcoICRI GAG'CTC

Cuts at:      0    1165    1884 
   Size:       1165     719
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     149     200    1127    1499    1618    1884 
   Size:        149      51     927     372     119     266

  Fragments arranged by size: 

                 927     372     266     149     119      51
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      50    1884 
   Size:         50    1834
 
EcoO109I rG'GnC_Cy

Cuts at:      0      19     777    1232    1884 
   Size:         19     758     455     652

  Fragments arranged by size: 

                 758     652     455      19
 
HaeII r_GCGC'y

Cuts at:      0     972    1802    1884 
   Size:        972     830      82

  Fragments arranged by size: 

                 972     830      82
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     122     154     214     246     273     305     385     417 
   Size:        122      32      60      32      27      32      80      32

Cuts at:    417    1851    1883    1884 
   Size:       1434      32       1

  Fragments arranged by size: 

                1434     122      80      60      32      32      32      32
                  32      27       1
 
KasI G'GCGC_C

Cuts at:      0    1798    1884 
   Size:       1798      86
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     175    1185    1884 
   Size:        175    1010     699

  Fragments arranged by size: 

                1010     699     175
 
MscI TGG'CCA

Cuts at:      0      26     520     657    1884 
   Size:         26     494     137    1227

  Fragments arranged by size: 

                1227     494     137      26
 
MslI CAynn'nnrTG

Cuts at:      0     455    1884 
   Size:        455    1429
 
MspA1I CmG'CkG

Cuts at:      0     109     875    1303    1884 
   Size:        109     766     428     581

  Fragments arranged by size: 

                 766     581     428     109
 
NarI GG'CG_CC

Cuts at:      0    1799    1884 
   Size:       1799      85
 
NcoI C'CATG_G

Cuts at:      0    1873    1884 
   Size:       1873      11
 
PfoI T'CCnGG_A

Cuts at:      0     126    1104    1884 
   Size:        126     978     780

  Fragments arranged by size: 

                 978     780     126
 
PmlI CAC'GTG

Cuts at:      0    1839    1884 
   Size:       1839      45
 
PpuMI rG'GwC_Cy

Cuts at:      0      19     777    1884 
   Size:         19     758    1107

  Fragments arranged by size: 

                1107     758      19
 
PsiI TTA'TAA

Cuts at:      0    1631    1884 
   Size:       1631     253
 
SacI G_AGCT'C

Cuts at:      0    1167    1884 
   Size:       1167     717
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1585    1884 
   Size:       1585     299
 
SfcI C'TryA_G

Cuts at:      0     426    1116    1884 
   Size:        426     690     768

  Fragments arranged by size: 

                 768     690     426
 
SfoI GGC'GCC

Cuts at:      0    1800    1884 
   Size:       1800      84
 
SpeI A'CTAG_T

Cuts at:      0     835    1884 
   Size:        835    1049
 
SspI AAT'ATT

Cuts at:      0    1625    1884 
   Size:       1625     259
 
StyI C'CwwG_G

Cuts at:      0    1329    1873    1884 
   Size:       1329     544      11

  Fragments arranged by size: 

                1329     544      11
 
SwaI ATTT'AAAT

Cuts at:      0     848    1884 
   Size:        848    1036
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     319     731    1884 
   Size:        319     412    1153

  Fragments arranged by size: 

                1153     412     319
 
TatI w'GTAC_w

Cuts at:      0     358    1703    1884 
   Size:        358    1345     181

  Fragments arranged by size: 

                1345     358     181
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1411    1884 
   Size:       1411     473
 
ZraI GAC'GTC

Cuts at:      0    1452    1827    1884 
   Size:       1452     375      57

  Fragments arranged by size: 

                1452     375      57
 

 Enzymes that do cut:

    AatII     AccI     AcuI    AlwNI     ApoI     AseI     AvaI    AvrII
     BanI    BanII     BbeI     BbsI    BciVI    BglII     BlpI     BmrI
     BplI     BpmI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspEI
    BspHI    BsrBI    BsrDI    BsrFI    BsrGI   BstAPI    BstXI    BstYI
     BtgI     BtsI     DraI     DrdI     EaeI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I    HaeII    Hin4I     KasI     MmeI     MscI
     MslI   MspA1I     NarI     NcoI     PfoI     PmlI    PpuMI     PsiI
     SacI     SapI     SfcI     SfoI     SpeI     SspI     StyI     SwaI
    TaqII     TatI     XcmI     ZraI

 Enzymes that do not cut: 

     AarI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     AloI     ApaI    ApaLI     AscI    AsiSI     BaeI
    BamHI    BbvCI     BcgI     BclI    BfrBI     BglI Bme1580I    BmgBI
     BmtI   Bpu10I    BpuEI    BsiWI     BsmI    BspMI   BssHII    BssSI
    BstBI   BstEII  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI   DraIII
     EagI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI   HincII
  HindIII     HpaI     KpnI     MfeI     MluI     NaeI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PmeI     PpiI    PshAI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII    SacII     SalI    SanDI     SbfI     ScaI
    SexAI     SfiI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SphI
     SrfI     StuI     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI
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