Restriction map for pF1KB5152
 (Circular) (Six-base) MAPSORT of: pF1KB5152.seq  Check: 9327  from: 1  to: 5075



pF1KB5152  5075 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:24  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   1561    1561 
   Size:       5075
 
AccI GT'mk_AC

Cuts at:   2113    2113 
   Size:       5075
 
Acc65I G'GTAC_C

Cuts at:   2091    2091 
   Size:       5075
 
AclI AA'CG_TT

Cuts at:   4915    4915 
   Size:       5075
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    156     315    1690    2900    3332    3685    4347     156 
   Size:        159    1375    1210     432     353     662     884

  Fragments arranged by size: 

                1375    1210     884     662     432     353     159
 
AflIII A'CryG_T

Cuts at:   1628    2427    4227    4508    1628 
   Size:        799    1800     281    2195

  Fragments arranged by size: 

                2195    1800     799     281
 
AlwNI CAG_nnn'CTG

Cuts at:   1194    1639    2380    3818    1194 
   Size:        445     741    1438    2451

  Fragments arranged by size: 

                2451    1438     741     445
 
ApaI G_GGCC'C

Cuts at:    518     821    1686     518 
   Size:        303     865    3907

  Fragments arranged by size: 

                3907     865     303
 
ApaLI G'TGCA_C

Cuts at:   3913    3913 
   Size:       5075
 
ApoI r'AATT_y

Cuts at:   2079    4428    4684    2079 
   Size:       2349     256    2470

  Fragments arranged by size: 

                2470    2349     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       5075
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       5075
 
AvaI C'yCGr_G

Cuts at:    511     798    1067    1217    1450    1679    1775    2095     511 
   Size:        287     269     150     233     229      96     320    3491

  Fragments arranged by size: 

                3491     320     287     269     233     229     150      96
            
 
BamHI G'GATC_C

Cuts at:   2100    2100 
   Size:       5075
 
BanI G'GyrC_C

Cuts at:    129     340     424    1234    1516    1573    2091    2505    2753 
   Size:        211      84     810     282      57     518     414     248

Cuts at:   2753    2788     129 
   Size:         35    2416

  Fragments arranged by size: 

                2416     810     518     414     282     248     211      84
                  57      35
 
BanII G_rGCy'C

Cuts at:    155     518     788     821     838     968    1066    1183    1230 
   Size:        363     270      33      17     130      98     117      47

Cuts at:   1230    1686    2089    3119     155 
   Size:        456     403    1030    2111

  Fragments arranged by size: 

                2111    1030     456     403     363     270     130     117
                  98      47      33      17
 
BbeI G_GCGC'C

Cuts at:    133     428    2509    2757     133 
   Size:        295    2081     248    2451

  Fragments arranged by size: 

                2451    2081     295     248
 
BbsI GAAGACnn'nnnn_

Cuts at:    637    1295     637 
   Size:        658    4417
 
BbvCI CC'TCA_GC

Cuts at:    777     990     777 
   Size:        213    4862
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1100    1134    1100 
   Size:         34    5041
 
BciVI GTATCCnnnnn_n'

Cuts at:   2973    4029    5075    2973 
   Size:       1056    1046    2973

  Fragments arranged by size: 

                2973    1056    1046
 
BclI T'GATC_A

Cuts at:   2595    2595 
   Size:       5075
 
BglI GCCn_nnn'nGGC

Cuts at:    226     809     875    1061    1466    1514    1582    1681     226 
   Size:        583      66     186     405      48      68      99    3620

  Fragments arranged by size: 

                3620     583     405     186      99      68      66      48
            
 
BglII A'GATC_T

Cuts at:   2590    4627    2590 
   Size:       2037    3038
 
BlpI GC'TnA_GC

Cuts at:   2187    2187 
   Size:       5075
 
Bme1580I G_kGCm'C

Cuts at:    142     343     518     821    1339    1686    2066    2700    2793 
   Size:        201     175     303     518     347     380     634      93

Cuts at:   2793    3917     142 
   Size:       1124    1300

  Fragments arranged by size: 

                1300    1124     634     518     380     347     303     201
                 175      93
 
BmgBI CAC'GTC

Cuts at:   1631    1631 
   Size:       5075
 
BmrI ACTGGGnnnn_n'

Cuts at:    543    2287    2368    2703    4847     543 
   Size:       1744      81     335    2144     771

  Fragments arranged by size: 

                2144    1744     771     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3442    3442 
   Size:       5075
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    810    1005     810 
   Size:        195    4880
 
Bpu10I CC'TnA_GC

Cuts at:    273     672     777     990    2038     273 
   Size:        399     105     213    1048    3310

  Fragments arranged by size: 

                3310    1048     399     213     105
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    277    1125    2251    3130    3844    4142     277 
   Size:        848    1126     879     714     298    1210

  Fragments arranged by size: 

                1210    1126     879     848     714     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   1267    1994    4779    1267 
   Size:        727    2785    1563

  Fragments arranged by size: 

                2785    1563     727
 
BsaAI yAC'GTr

Cuts at:    753    1007    1314    2428     753 
   Size:        254     307    1114    3400

  Fragments arranged by size: 

                3400    1114     307     254
 
BsaBI GATnn'nnATC

Cuts at:      6    2589    4626       6 
   Size:       2583    2037     455

  Fragments arranged by size: 

                2583    2037     455
 
BsaHI Gr'CG_yC

Cuts at:    130     425    1016    2506    2754    3456    4952     130 
   Size:        295     591    1490     248     702    1496     253

  Fragments arranged by size: 

                1496    1490     702     591     295     253     248
 
BsaWI w'CCGG_w

Cuts at:    626    2264    2785    3463    3874    4021    4295     626 
   Size:       1638     521     678     411     147     274    1406

  Fragments arranged by size: 

                1638    1406     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    544     574     938     968    1615    1645     544 
   Size:         30     364      30     647      30    3974

  Fragments arranged by size: 

                3974     647     364      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    297     978    1621    1636    1666    1669     297 
   Size:        681     643      15      30       3    3703

  Fragments arranged by size: 

                3703     681     643      30      15       3
 
BseYI C'CCAG_C

Cuts at:    268     319     667     724     825    1246    1410    1504    1821 
   Size:         51     348      57     101     421     164      94     317

Cuts at:   1821    2500    3258    3923    4331     268 
   Size:        679     758     665     408    1012

  Fragments arranged by size: 

                1012     758     679     665     421     408     348     317
                 164     101      94      57      51
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    754     895    1825    1867     754 
   Size:        141     930      42    3962

  Fragments arranged by size: 

                3962     930     141      42
 
BsiEI CG_ry'CG

Cuts at:    104    1586    2617    2663    3893     104 
   Size:       1482    1031      46    1230    1286

  Fragments arranged by size: 

                1482    1286    1230    1031      46
 
BsiHKAI G_wGCw'C

Cuts at:    155     788    1319    2089    2867    3057    3917     155 
   Size:        633     531     770     778     190     860    1313

  Fragments arranged by size: 

                1313     860     778     770     633     531     190
 
BsiWI C'GTAC_G

Cuts at:   1007    1007 
   Size:       5075
 
BsmBI CGTCTCn'nnnn_

Cuts at:    440    1023     440 
   Size:        583    4492
 
Bsp1286I G_dGCh'C

Cuts at:    142     155     343     518     788     821     838     968    1066 
   Size:         13     188     175     270      33      17     130      98

Cuts at:   1066    1183    1230    1319    1339    1686    2066    2089    2700 
   Size:        117      47      89      20     347     380      23     611

Cuts at:   2700    2793    2867    3057    3119    3917     142 
   Size:         93      74     190      62     798    1300

  Fragments arranged by size: 

                1300     798     611     380     347     270     190     188
                 175     130     117      98      93      89      74      62
                  47      33      23      20      17      13
 
BspEI T'CCGG_A

Cuts at:    626     626 
   Size:       5075
 
BspHI T'CATG_A

Cuts at:   3507    3507 
   Size:       5075
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1561    2125    2339    2641    3022    1561 
   Size:        564     214     302     381    3614

  Fragments arranged by size: 

                3614     564     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50     413     420     589    1743    1782    1932    3367    3421 
   Size:        363       7     169    1154      39     150    1435      54

Cuts at:   3421    4905    5070      50 
   Size:       1484     165      55

  Fragments arranged by size: 

                1484    1435    1154     363     169     165     150      55
                  54      39       7
 
BsrDI GCAATG_nn'

Cuts at:   2987    2987 
   Size:       5075
 
BsrFI r'CCGG_y

Cuts at:    212     263    1342    1396    3073    3254     212 
   Size:         51    1079      54    1677     181    2033

  Fragments arranged by size: 

                2033    1677    1079     181      54      51
 
BssSI C'ACGA_G

Cuts at:    919    3346    4054     919 
   Size:       2427     708    1940

  Fragments arranged by size: 

                2427    1940     708
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1359    1923    2452    1359 
   Size:        564     529    3982

  Fragments arranged by size: 

                3982     564     529
 
BstBI TT'CG_AA

Cuts at:   3436    3436 
   Size:       5075
 
BstXI CCAn_nnnn'nTGG

Cuts at:    387     943    1410     387 
   Size:        556     467    4052

  Fragments arranged by size: 

                4052     556     467
 
BstYI r'GATC_y

Cuts at:      1    2100    2567    2590    2925    3171    3477    3489    3575 
   Size:       2099     467      23     335     246     306      12      86

Cuts at:   3575    4627       1 
   Size:       1052     449

  Fragments arranged by size: 

                2099    1052     467     449     335     306     246      86
                  23      12
 
Bsu36I CC'TnA_GG

Cuts at:   1386    1386 
   Size:       5075
 
BtgI C'CryG_G

Cuts at:   1175    1266    1331    1371    1577    1175 
   Size:         91      65      40     206    4673

  Fragments arranged by size: 

                4673     206      91      65      40
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   3025    3206    3369    3025 
   Size:        181     163    4731

  Fragments arranged by size: 

                4731     181     163
 
BtsI GCAGTG_nn'

Cuts at:    888    2318     888 
   Size:       1430    3645
 
DraI TTT'AAA

Cuts at:   2074    2074 
   Size:       5075
 
DraIII CAC_nnn'GTG

Cuts at:   1332    1332 
   Size:       5075
 
DrdI GACnn_nn'nnGTC

Cuts at:   2781    4125    2781 
   Size:       1344    3731
 
EaeI y'GGCC_r

Cuts at:    227    1368    1583    1763    2660    2834    3225    3252    4597 
   Size:       1141     215     180     897     174     391      27    1345

Cuts at:   4597     227 
   Size:        705

  Fragments arranged by size: 

                1345    1141     897     705     391     215     180     174
                  27
 
EagI C'GGCC_G

Cuts at:   1583    2660    1583 
   Size:       1077    3998
 
EarI CTCTTCn'nnn_

Cuts at:    378    1967    3098    3308     378 
   Size:       1589    1131     210    2145

  Fragments arranged by size: 

                2145    1589    1131     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    597     702    3283    4025    4171    4885     597 
   Size:        105    2581     742     146     714     787

  Fragments arranged by size: 

                2581     787     742     714     146     105
 
EcoICRI GAG'CTC

Cuts at:    153     786    2087     153 
   Size:        633    1301    3141

  Fragments arranged by size: 

                3141    1301     633
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    156     315     810    1005    1690    2900    3332    3685    4347 
   Size:        159     495     195     685    1210     432     353     662

Cuts at:   4347     156 
   Size:        884

  Fragments arranged by size: 

                1210     884     685     662     495     432     353     195
                 159
 
EcoNI CCTnn'n_nnAGG

Cuts at:    469    1220    1300    1885     469 
   Size:        751      80     585    3659

  Fragments arranged by size: 

                3659     751     585      80
 
EcoO109I rG'GnC_Cy

Cuts at:    515     542     633    1191    1674    2214     515 
   Size:         27      91     558     483     540    3376

  Fragments arranged by size: 

                3376     558     540     483      91      27
 
EcoRI G'AATT_C

Cuts at:   2079    2079 
   Size:       5075
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   3094    3094 
   Size:       5075
 
FseI GG_CCGG'CC

Cuts at:    216     216 
   Size:       5075
 
FspI TGC'GCA

Cuts at:   2856    2856 
   Size:       5075
 
HaeII r_GCGC'y

Cuts at:    133     428    1381    1758    2509    2757    3987    4758     133 
   Size:        295     953     377     751     248    1230     771     450

  Fragments arranged by size: 

                1230     953     771     751     450     377     295     248
            
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    383     415    1615    1647    2917    2947    2949    2979     383 
   Size:         32    1200      32    1270      30       2      30    2479

  Fragments arranged by size: 

                2479    1270    1200      32      32      30      30       2
            
 
HincII GTy'rAC

Cuts at:   2114    2114 
   Size:       5075
 
KasI G'GCGC_C

Cuts at:    129     424    2505    2753     129 
   Size:        295    2081     248    2451

  Fragments arranged by size: 

                2451    2081     295     248
 
KpnI G_GTAC'C

Cuts at:   2095    2095 
   Size:       5075
 
MluI A'CGCG_T

Cuts at:   4508    4508 
   Size:       5075
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1416    1977    3834    4018    1416 
   Size:        561    1857     184    2473

  Fragments arranged by size: 

                2473    1857     561     184
 
MscI TGG'CCA

Cuts at:   1370    1765    2836    1370 
   Size:        395    1071    3609

  Fragments arranged by size: 

                3609    1071     395
 
MslI CAynn'nnrTG

Cuts at:   1312    3064    3191    1312 
   Size:       1752     127    3196

  Fragments arranged by size: 

                3196    1752     127
 
MspA1I CmG'CkG

Cuts at:    189     290     846     939    1043    1081    1636    2186    2500 
   Size:        101     556      93     104      38     555     550     314

Cuts at:   2500    2860    3644    3889     189 
   Size:        360     784     245    1375

  Fragments arranged by size: 

                1375     784     556     555     550     360     314     245
                 104     101      93      38
 
NaeI GCC'GGC

Cuts at:    214    1344    3256     214 
   Size:       1130    1912    2033

  Fragments arranged by size: 

                2033    1912    1130
 
NarI GG'CG_CC

Cuts at:    130     425    2506    2754     130 
   Size:        295    2081     248    2451

  Fragments arranged by size: 

                2451    2081     295     248
 
NgoMIV G'CCGG_C

Cuts at:    212    1342    3254     212 
   Size:       1130    1912    2033

  Fragments arranged by size: 

                2033    1912    1130
 
NotI GC'GGCC_GC

Cuts at:   1583    1583 
   Size:       5075
 
NspI r_CATG'y

Cuts at:   1353    1833    2128    2628    4231    1353 
   Size:        480     295     500    1603    2197

  Fragments arranged by size: 

                2197    1603     500     480     295
 
PasI CC'CwG_GG

Cuts at:   1188    1188 
   Size:       5075
 
PciI A'CATG_T

Cuts at:   4227    4227 
   Size:       5075
 
PflMI CCAn_nnn'nTGG

Cuts at:   1194    1332    1194 
   Size:        138    4937
 
PfoI T'CCnGG_A

Cuts at:    164     164 
   Size:       5075
 
PmeI GTTT'AAAC

Cuts at:   2074    2074 
   Size:       5075
 
PmlI CAC'GTG

Cuts at:   1314    1314 
   Size:       5075
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3521    3553    3521 
   Size:         32    5043
 
PpuMI rG'GwC_Cy

Cuts at:    542     633     542 
   Size:         91    4984
 
PshAI GACnn'nnGTC

Cuts at:   1259    1259 
   Size:       5075
 
PspOMI G'GGCC_C

Cuts at:    514     817    1682     514 
   Size:        303     865    3907

  Fragments arranged by size: 

                3907     865     303
 
PspXI vC'TCGA_Gb

Cuts at:   1217    1217 
   Size:       5075
 
PstI C_TGCA'G

Cuts at:   1277    2122    2807    1277 
   Size:        845     685    3545

  Fragments arranged by size: 

                3545     845     685
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       5075
 
PvuII CAG'CTG

Cuts at:    939    1081    2500    2860     939 
   Size:        142    1419     360    3154

  Fragments arranged by size: 

                3154    1419     360     142
 
RsrII CG'GwC_CG

Cuts at:   3270    3270 
   Size:       5075
 
SacI G_AGCT'C

Cuts at:    155     788    2089     155 
   Size:        633    1301    3141

  Fragments arranged by size: 

                3141    1301     633
 
SalI G'TCGA_C

Cuts at:   2112    2112 
   Size:       5075
 
SapI GCTCTTCn'nnn_

Cuts at:   3098    3308    3098 
   Size:        210    4865
 
SbfI CC_TGCA'GG

Cuts at:   2122    2122 
   Size:       5075
 
ScaI AGT'ACT

Cuts at:    553    4592     553 
   Size:       4039    1036
 
SfcI C'TryA_G

Cuts at:     33    1273    1551    2118    2803    3771    3962      33 
   Size:       1240     278     567     685     968     191    1146

  Fragments arranged by size: 

                1240    1146     968     685     567     278     191
 
SfiI GGCCn_nnn'nGGCC

Cuts at:   1681    1681 
   Size:       5075
 
SfoI GGC'GCC

Cuts at:    131     426    2507    2755     131 
   Size:        295    2081     248    2451

  Fragments arranged by size: 

                2451    2081     295     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       5075
 
SmaI CCC'GGG

Cuts at:    513     800    1681    1777    2097     513 
   Size:        287     881      96     320    3491

  Fragments arranged by size: 

                3491     881     320     287      96
 
SmlI C'TyrA_G

Cuts at:    256    1140    1217    2230    3145    3859    4121     256 
   Size:        884      77    1013     915     714     262    1210

  Fragments arranged by size: 

                1210    1013     915     884     714     262      77
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       5075
 
SphI G_CATG'C

Cuts at:   2128    2628    2128 
   Size:        500    4575
 
StuI AGG'CCT

Cuts at:   1430    1810    2036    1430 
   Size:        380     226    4469

  Fragments arranged by size: 

                4469     380     226
 
StyI C'CwwG_G

Cuts at:   2209    2563    2209 
   Size:        354    4721
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    358     681     817    1402    1887    2045    2658    2791    3250 
   Size:        323     136     585     485     158     613     133     459

Cuts at:   3250    3459     358 
   Size:        209    1974

  Fragments arranged by size: 

                1974     613     585     485     459     323     209     158
                 136     133
 
TatI w'GTAC_w

Cuts at:    551    4590     551 
   Size:       4039    1036
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    938     970    4592    4624     938 
   Size:         32    3622      32    1389

  Fragments arranged by size: 

                3622    1389      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   2872    2872 
   Size:       5075
 
XbaI T'CTAG_A

Cuts at:   2106    2106 
   Size:       5075
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2570    2570 
   Size:       5075
 
XhoI C'TCGA_G

Cuts at:   1217    1217 
   Size:       5075
 
XmaI C'CCGG_G

Cuts at:    511     798    1679    1775    2095     511 
   Size:        287     881      96     320    3491

  Fragments arranged by size: 

                3491     881     320     287      96
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI   AflIII    AlwNI     ApaI
    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI    BstXI
    BstYI   Bsu36I     BtgI    BtgZI     BtsI     DraI   DraIII     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI     FalI     FseI     FspI    HaeII    Hin4I   HincII     KasI
     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
   NgoMIV     NotI     NspI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI   PspOMI    PspXI     PstI     PvuI
    PvuII    RsrII     SacI     SalI     SapI     SbfI     ScaI     SfcI
     SfiI     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     StuI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI

 Enzymes that do not cut: 

    AatII     AfeI    AflII     AgeI     AhdI     AleI     AlfI     AloI
     AscI    AvrII     BaeI    BfrBI     BmtI     BsmI    BsrGI   BssHII
   BstEII  BstZ17I     ClaI    CspCI    EcoRV    FspAI  HindIII     HpaI
     MfeI     NcoI     NdeI     NheI     NruI     NsiI     PacI     PsiI
     PsrI    SacII    SanDI    SexAI    SgrAI    SnaBI     SrfI     SspI
     SwaI     XmnI     ZraI
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