Restriction map for pF1KB5122
##################################
# NO CUT ENZYMES (  142 enzymes) #
##################################

AatII       AccII       AccIII      AclI        AcyI        
AflII       AflIII      AgeI        AhdI        Alw44I      
Aor51HI     ApaI        ApaLI       AscI        Asp700I     
AspEI       AspI        AvaI        AviII       AvrII       
BalI        BamHI       BanII       BbeI        BbrPI       
BbsI        BbvCI       BciVI       BclI        BcnI        
BfrI        BglI        BglII       BlnI        BlpI        
Bpu1102I    BpuAI       BsaAI       BsaHI       BsaI        
BsaWI       BseAI       BsgI        BsiEI       BsiWI       
BsmBI       BsoBI       BspDI       BspEI       BspHI       
BspLU11I    BsrBI       BsrDI       BsrFI       BssHII      
BssSI       BstBI       BstUI       BstXI       CelII       
CfoI        Cfr10I      ClaI        CpoI        DraIII      
DsaI        EaeI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      EcoNI       EcoRI       
EcoRV       FbaI        FseI        FspI        HaeII       
HapII       HhaI        Hin1I       HinP1I      HpaI        
HpaII       KasI        Ksp632I     KspI        MaeII       
MboII       MluI        MluNI       MroI        MscI        
MspI        MvnI        NaeI        NarI        NciI        
NcoI        NdeI        NgoMIV      NotI        NruI        
NspI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
Psp1406I    PvuI        RcaI        RsrII       SacI        
SacII       SapI        SbfI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    StuI        
SwaI        Tth111I     XcmI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

Acc65I        208  GGTACC               G^GTACC
AlwI          364  GGATC                GGATC(4/5)
AocI          782  CCTTAGG              CC^T[ACGT]AGG
AseI          196  ATTAAT               AT^TAAT
AsnI          196  ATTAAT               AT^TAAT
Asp718I       208  GGTACC               G^GTACC
BcgI         1291  CGAGGGATATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BseRI         400  GAGGAG               GAGGAG(10/8)
BspMI        1361  GCAGGT               ACCTGC(4/8)
Bst1107I      641  GTATAC               GTA^TAC
BstEII        766  GGTAACC              G^GT[ACGT]ACC
BstPI         766  GGTAACC              G^GT[ACGT]ACC
BstYI         364  GGATCT               [GA]^GATC[CT]
BstZ17I       641  GTATAC               GTA^TAC
Bsu36I        782  CCTTAGG              CC^T[ACGT]AGG
DraI         1116  TTTAAA               TTT^AAA
DrdI         1141  GACAATTGTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I        782  CCTTAGG              CC^T[ACGT]AGG
EcoO65I       766  GGTAACC              G^GT[ACGT]ACC
EcoT14I      1035  CCAAGG               C^C[AT][AT]GG
EcoT22I      1299  ATGCAT               ATGCA^T
HgaI          880  GACGC                GACGC(5/10)
HphI          892  GGTGA                GGTGA(8/7)
KpnI          208  GGTACC               GGTAC^C
MfeI         1143  CAATTG               C^AATTG
MflI          364  GGATCT               [GA]^GATC[CT]
MunI         1143  CAATTG               C^AATTG
NheI          775  GCTAGC               G^CTAGC
NsiI         1299  ATGCAT               ATGCA^T
PflMI         746  CCAGAATATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI        1070  GGGACCC              [GA]G^G[AT]CC[CT]
PshBI         196  ATTAAT               AT^TAAT
SalI          224  GTCGAC               G^TCGAC
ScaI          627  AGTACT               AGT^ACT
SspI         1455  AATATT               AAT^ATT
StyI         1035  CCAAGG               C^C[AT][AT]GG
TaqI          225  TCGA                 T^CGA
Tsp45I        682  GTCAC                ^GT[GC]AC
TthHB8I       225  TCGA                 T^CGA
Van91I        746  CCAGAATATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI          690  TCTAGA               T^CTAGA
XhoII         364  GGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   33 enzymes) #
###################################

AcsI          834  AAATTT               [GA]^AATT[CT]
             1178  AAATTT              
ApoI          834  AAATTT               [GA]^AATT[CT]
             1178  AAATTT              
AspHI         410  GAGCAC               G[AT]GC[AT]^C
             1421  GTGCTC              
AvaII        1071  GGACC                G^G[AT]CC
             1207  GGACC               
BanI          121  GGTGCC               G^G[CT][GA]CC
              208  GGTACC              
BpmI          563  CTGGAG               CTGGAG(16/14)
              851  CTGGAG              
BsaBI         147  GATTTCTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1296  GATATGCATC          
BsiHKAI       410  GAGCAC               G[AT]GC[AT]^C
             1421  GTGCTC              
BsiYI         731  CCTATGATGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              746  CCAGAATATGG         
BslI          731  CCTATGATGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              746  CCAGAATATGG         
BsmI         1473  GAATGC               GAATGC(1/-1)
              989  GCATTC              
Bsp1407I     1334  TGTACA               T^GTACA
             1395  TGTACA              
BsrGI        1334  TGTACA               T^GTACA
             1395  TGTACA              
BssKI         527  CCTGG                ^CC[ACGT]GG
             1210  CCAGG               
BstAPI        322  GCAGCAGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              325  GCAGCTGCTGC         
BstNI         527  CCTGG                CC^[AT]GG
             1210  CCAGG               
DpnII         365  GATC                 ^GATC
              544  GATC                
DraII         798  AGGCCCT              [GA]G^G[ACGT]CC[CT]
             1070  GGGACCC             
EcoO109I      798  AGGCCCT              [GA]G^G[ACGT]CC[CT]
             1070  GGGACCC             
EcoRII        527  CCTGG                ^CC[AT]GG
             1210  CCAGG               
FauI          136  CCCGC                CCCGC(4/6)
              495  GCGGG               
HincII         22  GTCAAC               GT[CT]^[GA]AC
              224  GTCGAC              
HindII         22  GTCAAC               GT[CT]^[GA]AC
              224  GTCGAC              
HindIII       290  AAGCTT               A^AGCTT
              304  AAGCTT              
MaeIII        682  GTCAC                ^GT[ACGT]AC
              767  GTAAC               
MamI          147  GATTTCTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1296  GATATGCATC          
MboI          365  GATC                 ^GATC
              544  GATC                
MvaI          527  CCTGG                CC^[AT]GG
             1210  CCAGG               
NdeII         365  GATC                 ^GATC
              544  GATC                
PstI          332  CTGCAG               CTGCA^G
              347  CTGCAG              
Sau3AI        365  GATC                 ^GATC
              544  GATC                
ScrFI         527  CCTGG                CC^[ACGT]GG
             1210  CCAGG               
SspBI        1334  TGTACA               T^GTACA
             1395  TGTACA              


#########################################
# 3 OR MORE CUT ENZYMES (   41 enzymes) #
#########################################

AccI        AciI        AfaI        AluI        AlwNI       
BbvI        BfaI        BmyI        BsaJI       BsmAI       
BsmFI       Bsp1286I    BsrI        BstF5I      Cac8I       
Cfr13I      DdeI        Fnu4HI      FokI        HaeIII      
HinfI       ItaI        MaeI        MnlI        MseI        
MslI        MspA1I      MwoI        NlaIII      NlaIV       
PleI        PvuII       RsaI        Sau96I      SfaNI       
SfcI        TfiI        Tru9I       TseI        Tsp509I     
TspRI       
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