Restriction map for pF1KB5120
##################################
# NO CUT ENZYMES (  144 enzymes) #
##################################

AatII       Acc65I      AccII       AclI        AcyI        
AflII       AflIII      AgeI        AhdI        AlwNI       
AocI        Aor51HI     ApaI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspI        
AvaI        AviII       AvrII       BalI        BbeI        
BbrPI       BbvCI       BcgI        BciVI       BclI        
BcnI        BfrI        BglI        BglII       BlnI        
BsaAI       BsaBI       BsaHI       BsiEI       BsiWI       
BsmBI       BsmI        BsoBI       Bsp1407I    BspDI       
BspLU11I    BsrBI       BsrDI       BsrGI       BssHII      
BssSI       BstAPI      BstBI       BstEII      BstPI       
BstUI       Bsu36I      CfoI        ClaI        CpoI        
DraIII      EaeI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO65I     
EcoRI       EcoRV       EcoT22I     FauI        FbaI        
FseI        FspI        HaeII       HhaI        Hin1I       
HinP1I      HincII      HindII      HpaI        KasI        
KpnI        KspI        MaeII       MamI        MluI        
MluNI       MscI        MvnI        NaeI        NarI        
NciI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PleI        PmaCI       PmeI        PmlI        PshBI       
Psp1406I    PstI        PvuI        RsrII       SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
StuI        Tth111I     Van91I      XbaI        XcmI        
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AccIII        405  TCCGGA               T^CCGGA
AfaI          660  GTAC                 GT^AC
Alw44I       1004  GTGCAC               G^TGCAC
ApaLI        1004  GTGCAC               G^TGCAC
BamHI         567  GGATCC               G^GATCC
BanI          669  GGCACC               G^G[CT][GA]CC
BanII         289  GAGCTC               G[GA]GC[CT]^C
BbsI          910  GTCTTC               GAAGAC(2/6)
BlpI          742  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      742  GCTGAGC              GC^T[ACGT]AGC
BpuAI         910  GTCTTC               GAAGAC(2/6)
BsaI         1208  GAGACC               GGTCTC(1/5)
BseAI         405  TCCGGA               T^CCGGA
BsmFI        1031  GGGAC                GGGAC(10/14)
BspEI         405  TCCGGA               T^CCGGA
BspHI         299  TCATGA               T^CATGA
BspMI        1068  ACCTGC               ACCTGC(4/8)
BsrFI          45  GCCGGT               [GA]^CCGG[CT]
BstXI         516  CCATTCCTTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         567  GGATCC               [GA]^GATC[CT]
CelII         742  GCTGAGC              GC^T[ACGT]AGC
Cfr10I         45  GCCGGT               [GA]^CCGG[CT]
DraI          871  TTTAAA               TTT^AAA
DsaI         1212  CCGTGG               C^C[GA][CT]GG
EarI         1075  CTCTTC               CTCTTC(1/4)
EcoT14I       898  CCTTGG               C^C[AT][AT]GG
HindIII       998  AAGCTT               A^AGCTT
Ksp632I      1075  CTCTTC               CTCTTC(1/4)
MfeI          938  CAATTG               C^AATTG
MflI          567  GGATCC               [GA]^GATC[CT]
MroI          405  TCCGGA               T^CCGGA
MslI          174  CACTAAAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI          938  CAATTG               C^AATTG
PshAI         903  GACTAAGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI          299  TCATGA               T^CATGA
RsaI          660  GTAC                 GT^AC
SacI          289  GAGCTC               GAGCT^C
SmlI         1091  CTCAAG               C^T[CT][GA]AG
SspI          765  AATATT               AAT^ATT
StyI          898  CCTTGG               C^C[AT][AT]GG
SwaI          870  ATTTAAAT             ATTT^AAAT
XhoII         567  GGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AccI          415  GTATAC               GT^[AC][GT]AC
              966  GTATAC              
AcsI          868  GAATTT               [GA]^AATT[CT]
              874  AAATTC              
ApoI          868  GAATTT               [GA]^AATT[CT]
              874  AAATTC              
AspHI         289  GAGCTC               G[AT]GC[AT]^C
             1004  GTGCAC              
BsaWI         405  TCCGGA               [AT]^CCGG[AT]
              930  ACCGGA              
BseRI        1136  CTCCTC               GAGGAG(10/8)
              626  GAGGAG              
BsgI         1070  CTGCAC               GTGCAG(16/14)
               49  GTGCAG              
BsiHKAI       289  GAGCTC               G[AT]GC[AT]^C
             1004  GTGCAC              
Bst1107I      415  GTATAC               GTA^TAC
              966  GTATAC              
BstF5I        895  CATCC                GGATG(2/0)
             1125  CATCC               
BstZ17I       415  GTATAC               GTA^TAC
              966  GTATAC              
Cac8I          41  GCAAGC               GC[ACGT]^[ACGT]GC
              727  GCTGGC              
DdeI          743  CTGAG                C^T[ACGT]AG
              905  CTAAG               
DraII         812  AGGTCCT              [GA]G^G[ACGT]CC[CT]
             1031  GGGACCC             
DrdI          431  GACACTATAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1033  GACCCAAATGTC        
EcoO109I      812  AGGTCCT              [GA]G^G[ACGT]CC[CT]
             1031  GGGACCC             
FokI          895  CATCC                GGATG(9/13)
             1125  CATCC               
HaeIII         11  GGCC                 GG^CC
              149  GGCC                
HgaI          453  GACGC                GACGC(5/10)
             1189  GCGTC               
PpuMI         812  AGGTCCT              [GA]G^G[AT]CC[CT]
             1031  GGGACCC             
SfaNI        1195  GATGC                GCATC(5/9)
             1240  GCATC               
SfcI          435  CTATAG               C^T[GA][CT]AG
              663  CTATAG              
TaqI          285  TCGA                 T^CGA
              441  TCGA                
Tsp45I        109  GTGAC                ^GT[GC]AC
              596  GTGAC               
TthHB8I       285  TCGA                 T^CGA
              441  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   47 enzymes) #
#########################################

AciI        AluI        AlwI        AvaII       BbvI        
BfaI        BmyI        BpmI        BsaJI       BsiYI       
BslI        BsmAI       Bsp1286I    BsrI        BssKI       
BstNI       Cfr13I      DpnII       EcoRII      Fnu4HI      
HapII       HinfI       HpaII       HphI        ItaI        
MaeI        MaeIII      MboI        MboII       MnlI        
MseI        MspA1I      MspI        MvaI        MwoI        
NdeII       NlaIII      NlaIV       PvuII       Sau3AI      
Sau96I      ScrFI       TfiI        Tru9I       TseI        
Tsp509I     TspRI       
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