Restriction map for pF1KB5104
 (Linear) (Six-base) MAPSORT of: KIBB5104.seq  Check: 5689  from: 1  to: 1644



KIBB5104.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:26  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      35     125     178     271    1644 
   Size:         35      90      53      93    1373

  Fragments arranged by size: 

                1373      93      90      53      35
 
AflIII A'CryG_T

Cuts at:      0     664    1644 
   Size:        664     980
 
AleI CACnn'nnGTG

Cuts at:      0     253    1644 
   Size:        253    1391
 
AlwNI CAG_nnn'CTG

Cuts at:      0     294     297    1644 
   Size:        294       3    1347

  Fragments arranged by size: 

                1347     294       3
 
ApoI r'AATT_y

Cuts at:      0     185     304     421     655    1011    1557    1644 
   Size:        185     119     117     234     356     546      87

  Fragments arranged by size: 

                 546     356     234     185     119     117      87
 
AseI AT'TA_AT

Cuts at:      0    1202    1644 
   Size:       1202     442
 
BanI G'GyrC_C

Cuts at:      0      99    1356    1644 
   Size:         99    1257     288

  Fragments arranged by size: 

                1257     288      99
 
BanII G_rGCy'C

Cuts at:      0      69     995    1644 
   Size:         69     926     649

  Fragments arranged by size: 

                 926     649      69
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1088    1644 
   Size:       1088     556
 
BbvCI CC'TCA_GC

Cuts at:      0     416    1644 
   Size:        416    1228
 
BclI T'GATC_A

Cuts at:      0     407    1644 
   Size:        407    1237
 
Bme1580I G_kGCm'C

Cuts at:      0     102    1317    1644 
   Size:        102    1215     327

  Fragments arranged by size: 

                1215     327     102
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1328    1570    1644 
   Size:       1328     242      74

  Fragments arranged by size: 

                1328     242      74
 
Bpu10I CC'TnA_GC

Cuts at:      0     416    1644 
   Size:        416    1228
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1111    1251    1644 
   Size:       1111     140     393

  Fragments arranged by size: 

                1111     393     140
 
BsaHI Gr'CG_yC

Cuts at:      0    1626    1644 
   Size:       1626      18
 
BsaWI w'CCGG_w

Cuts at:      0    1015    1644 
   Size:       1015     629
 
BseYI C'CCAG_C

Cuts at:      0     994    1644 
   Size:        994     650
 
BsiHKAI G_wGCw'C

Cuts at:      0      69    1644 
   Size:         69    1575
 
BsmI GAATG_Cn'

Cuts at:      0     353     453    1644 
   Size:        353     100    1191

  Fragments arranged by size: 

                1191     353     100
 
Bsp1286I G_dGCh'C

Cuts at:      0      69     102     995    1317    1644 
   Size:         69      33     893     322     327

  Fragments arranged by size: 

                 893     327     322      69      33
 
BspEI T'CCGG_A

Cuts at:      0    1015    1644 
   Size:       1015     629
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     297     736    1644 
   Size:        297     439     908

  Fragments arranged by size: 

                 908     439     297
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     535     942    1002    1644 
   Size:        535     407      60     642

  Fragments arranged by size: 

                 642     535     407      60
 
BstYI r'GATC_y

Cuts at:      0     360     720    1644 
   Size:        360     360     924

  Fragments arranged by size: 

                 924     360     360
 
BtgI C'CryG_G

Cuts at:      0     935    1644 
   Size:        935     709
 
BtsI GCAGTG_nn'

Cuts at:      0     260    1644 
   Size:        260    1384
 
DraI TTT'AAA

Cuts at:      0     440    1644 
   Size:        440    1204
 
EarI CTCTTCn'nnn_

Cuts at:      0     329    1571    1644 
   Size:        329    1242      73

  Fragments arranged by size: 

                1242     329      73
 
EcoICRI GAG'CTC

Cuts at:      0      67    1644 
   Size:         67    1577
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      35     125     178     271    1328    1570    1644 
   Size:         35      90      53      93    1057     242      74

  Fragments arranged by size: 

                1057     242      93      90      74      53      35
 
EcoO109I rG'GnC_Cy

Cuts at:      0     593    1644 
   Size:        593    1051
 
EcoRI G'AATT_C

Cuts at:      0     421     655    1644 
   Size:        421     234     989

  Fragments arranged by size: 

                 989     421     234
 
EcoRV GAT'ATC

Cuts at:      0     573    1644 
   Size:        573    1071
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     110     301     741    1644 
   Size:        110     191     440     903

  Fragments arranged by size: 

                 903     440     191     110
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     110     142    1357    1389    1644 
   Size:        110      32    1215      32     255

  Fragments arranged by size: 

                1215     255     110      32      32
 
HincII GTy'rAC

Cuts at:      0    1253    1644 
   Size:       1253     391
 
HindIII A'AGCT_T

Cuts at:      0    1235    1644 
   Size:       1235     409
 
HpaI GTT'AAC

Cuts at:      0    1253    1644 
   Size:       1253     391
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1180    1383    1644 
   Size:       1180     203     261

  Fragments arranged by size: 

                1180     261     203
 
MslI CAynn'nnrTG

Cuts at:      0     137     253     929    1391    1644 
   Size:        137     116     676     462     253

  Fragments arranged by size: 

                 676     462     253     137     116
 
MspA1I CmG'CkG

Cuts at:      0     198     294     949    1644 
   Size:        198      96     655     695

  Fragments arranged by size: 

                 695     655     198      96
 
NcoI C'CATG_G

Cuts at:      0     935    1644 
   Size:        935     709
 
NdeI CA'TA_TG

Cuts at:      0     134    1644 
   Size:        134    1510
 
NspI r_CATG'y

Cuts at:      0     211    1644 
   Size:        211    1433
 
PpuMI rG'GwC_Cy

Cuts at:      0     593    1644 
   Size:        593    1051
 
PsiI TTA'TAA

Cuts at:      0     383    1644 
   Size:        383    1261
 
PvuII CAG'CTG

Cuts at:      0     198     294     949    1644 
   Size:        198      96     655     695

  Fragments arranged by size: 

                 695     655     198      96
 
SacI G_AGCT'C

Cuts at:      0      69    1644 
   Size:         69    1575
 
SfcI C'TryA_G

Cuts at:      0    1174    1644 
   Size:       1174     470
 
SmlI C'TyrA_G

Cuts at:      0    1126    1266    1644 
   Size:       1126     140     378

  Fragments arranged by size: 

                1126     378     140
 
SpeI A'CTAG_T

Cuts at:      0     985    1644 
   Size:        985     659
 
StuI AGG'CCT

Cuts at:      0     758    1644 
   Size:        758     886
 
StyI C'CwwG_G

Cuts at:      0     935    1644 
   Size:        935     709
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1260    1644 
   Size:       1260     384
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     656     688    1644 
   Size:        656      32     956

  Fragments arranged by size: 

                 956     656      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1350    1644 
   Size:       1350     294
 
XmnI GAAnn'nnTTC

Cuts at:      0     629     655    1532    1644 
   Size:        629      26     877     112

  Fragments arranged by size: 

                 877     629     112      26
 

 Enzymes that do cut:

     AcuI   AflIII     AleI    AlwNI     ApoI     AseI     BanI    BanII
     BbsI    BbvCI     BclI Bme1580I     BpmI   Bpu10I    BpuEI    BsaHI
    BsaWI    BseYI  BsiHKAI     BsmI Bsp1286I    BspEI   BstAPI    BstXI
    BstYI     BtgI     BtsI     DraI     EarI  EcoICRI  Eco57MI EcoO109I
    EcoRI    EcoRV     FalI    Hin4I   HincII  HindIII     HpaI     MmeI
     MslI   MspA1I     NcoI     NdeI     NspI    PpuMI     PsiI    PvuII
     SacI     SfcI     SmlI     SpeI     StuI     StyI    TaqII     TstI
     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AlfI     AloI     ApaI    ApaLI     AscI    AsiSI     AvaI
    AvrII     BaeI    BamHI     BbeI     BcgI    BciVI    BfrBI     BglI
    BglII     BlpI    BmgBI     BmrI     BmtI     BplI     BsaI    BsaAI
    BsaBI    BsaXI    BseRI     BsgI    BsiEI    BsiWI    BsmBI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI    BstBI
   BstEII  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI   DraIII     DrdI
     EaeI     EagI     EciI    EcoNI     FseI     FspI    FspAI    HaeII
     KasI     KpnI     MfeI     MluI     MscI     NaeI     NarI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PshAI   PspOMI    PspXI     PsrI
     PstI     PvuI    RsrII    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SphI     SrfI     SspI     SwaI     TatI  Tth111I     XbaI     XhoI
     XmaI     ZraI
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