Restriction map for pF1KB5098
 (Linear) (Six-base) MAPSORT of: KIBB5098.seq  Check: 7687  from: 1  to: 3474



KIBB5098.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:26  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    2697    3474 
   Size:       2697     777
 
AccI GT'mk_AC

Cuts at:      0    1634    3474 
   Size:       1634    1840
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1036    1416    2565    3474 
   Size:       1036     380    1149     909

  Fragments arranged by size: 

                1149    1036     909     380
 
AflIII A'CryG_T

Cuts at:      0    1953    3474 
   Size:       1953    1521
 
AgeI A'CCGG_T

Cuts at:      0    1261    3474 
   Size:       1261    2213
 
AleI CACnn'nnGTG

Cuts at:      0    1516    3262    3474 
   Size:       1516    1746     212

  Fragments arranged by size: 

                1746    1516     212
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1280    1900    1984    3474 
   Size:       1280     620      84    1490

  Fragments arranged by size: 

                1490    1280     620      84
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1834    1866    3474 
   Size:       1834      32    1608

  Fragments arranged by size: 

                1834    1608      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1923    2352    3474 
   Size:       1923     429    1122

  Fragments arranged by size: 

                1923    1122     429
 
ApaLI G'TGCA_C

Cuts at:      0    2710    3474 
   Size:       2710     764
 
AseI AT'TA_AT

Cuts at:      0    2789    3474 
   Size:       2789     685
 
AvaI C'yCGr_G

Cuts at:      0     112     146     178     571     796    1846    2375    2841 
   Size:        112      34      32     393     225    1050     529     466

Cuts at:   2841    2883    3295    3319    3367    3474 
   Size:         42     412      24      48     107

  Fragments arranged by size: 

                1050     529     466     412     393     225     112     107
                  48      42      34      32      24
 
AvrII C'CTAG_G

Cuts at:      0    3129    3474 
   Size:       3129     345
 
BamHI G'GATC_C

Cuts at:      0    1868    3474 
   Size:       1868    1606
 
BanI G'GyrC_C

Cuts at:      0      18      68     312     597     752    1264    1688    1992 
   Size:         18      50     244     285     155     512     424     304

Cuts at:   1992    3107    3474 
   Size:       1115     367

  Fragments arranged by size: 

                1115     512     424     367     304     285     244     155
                  50      18
 
BanII G_rGCy'C

Cuts at:      0     146     185     818    1100    1532    1780    1847    2041 
   Size:        146      39     633     282     432     248      67     194

Cuts at:   2041    2486    2612    3200    3296    3320    3474 
   Size:        445     126     588      96      24     154

  Fragments arranged by size: 

                 633     588     445     432     282     248     194     154
                 146     126      96      67      39      24
 
BbeI G_GCGC'C

Cuts at:      0      22      72     601     756    3111    3474 
   Size:         22      50     529     155    2355     363

  Fragments arranged by size: 

                2355     529     363     155      50      22
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    2809    3474 
   Size:       2809     665
 
BbvCI CC'TCA_GC

Cuts at:      0    2135    3474 
   Size:       2135    1339
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1294    1328    2514    2548    3474 
   Size:       1294      34    1186      34     926

  Fragments arranged by size: 

                1294    1186     926      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    2229    2316    3474 
   Size:       2229      87    1158

  Fragments arranged by size: 

                2229    1158      87
 
BglII A'GATC_T

Cuts at:      0    1793    1808    3474 
   Size:       1793      15    1666

  Fragments arranged by size: 

                1793    1666      15
 
BlpI GC'TnA_GC

Cuts at:      0      28     983    3474 
   Size:         28     955    2491

  Fragments arranged by size: 

                2491     955      28
 
Bme1580I G_kGCm'C

Cuts at:      0      61     317    1236    1744    1997    2714    3093    3474 
   Size:         61     256     919     508     253     717     379     381

  Fragments arranged by size: 

                 919     717     508     381     379     256     253      61
            
 
BmgBI CAC'GTC

Cuts at:      0     159    3474 
   Size:        159    3315
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1317    1846    2064    3327    3474 
   Size:       1317     529     218    1263     147

  Fragments arranged by size: 

                1317    1263     529     218     147
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      83     556    1374    1624    2402    2626    3474 
   Size:         83     473     818     250     778     224     848

  Fragments arranged by size: 

                 848     818     778     473     250     224      83
 
Bpu10I CC'TnA_GC

Cuts at:      0     140     665    2135    3181    3474 
   Size:        140     525    1470    1046     293

  Fragments arranged by size: 

                1470    1046     525     293     140
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1774    3474 
   Size:       1774    1700
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     628     673    1048    2403    3244    3322    3346    3439 
   Size:        628      45     375    1355     841      78      24      93

Cuts at:   3439    3474 
   Size:         35

  Fragments arranged by size: 

                1355     841     628     375      93      78      45      35
                  24
 
BsaAI yAC'GTr

Cuts at:      0     918    1954    3474 
   Size:        918    1036    1520

  Fragments arranged by size: 

                1520    1036     918
 
BsaHI Gr'CG_yC

Cuts at:      0      19      69     598     753    1132    1367    3048    3063 
   Size:         19      50     529     155     379     235    1681      15

Cuts at:   3063    3108    3474 
   Size:         45     366

  Fragments arranged by size: 

                1681     529     379     366     235     155      50      45
                  19      15
 
BsaWI w'CCGG_w

Cuts at:      0     258    1261    2435    3474 
   Size:        258    1003    1174    1039

  Fragments arranged by size: 

                1174    1039    1003     258
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1111    1141    1834    1864    2383    2413    3132    3162 
   Size:       1111      30     693      30     519      30     719      30

Cuts at:   3162    3283    3307    3313    3331    3337    3361    3474 
   Size:        121      24       6      18       6      24     113

  Fragments arranged by size: 

                1111     719     693     519     121     113      30      30
                  30      30      24      24      18       6       6
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     118     589     646     688     691     804     838     865 
   Size:        118     471      57      42       3     113      34      27

Cuts at:    865     950    1024    1066    1126    1135    1138    1197    1750 
   Size:         85      74      42      60       9       3      59     553

Cuts at:   1750    2092    2152    3397    3400    3403    3406    3415    3418 
   Size:        342      60    1245       3       3       3       9       3

Cuts at:   3418    3474 
   Size:         56

  Fragments arranged by size: 

                1245     553     471     342     118     113      85      74
                  60      60      59      57      56      42      42      34
                  27       9       9       3       3       3       3       3
                   3
 
BseYI C'CCAG_C

Cuts at:      0     559     840    1611    1864    1887    2061    2271    3230 
   Size:        559     281     771     253      23     174     210     959

Cuts at:   3230    3356    3474 
   Size:        126     118

  Fragments arranged by size: 

                 959     771     559     281     253     210     174     126
                 118      23
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1990    3474 
   Size:       1990    1484
 
BsiEI CG_ry'CG

Cuts at:      0       8     245    1245    3474 
   Size:          8     237    1000    2229

  Fragments arranged by size: 

                2229    1000     237       8
 
BsiHKAI G_wGCw'C

Cuts at:      0     659    1532    1621    1780    2126    2612    2714    3187 
   Size:        659     873      89     159     346     486     102     473

Cuts at:   3187    3474 
   Size:        287

  Fragments arranged by size: 

                 873     659     486     473     346     287     159     102
                  89
 
BsmI GAATG_Cn'

Cuts at:      0    3424    3474 
   Size:       3424      50
 
Bsp1286I G_dGCh'C

Cuts at:      0      61     146     185     317     659     818    1100    1236 
   Size:         61      85      39     132     342     159     282     136

Cuts at:   1236    1532    1621    1744    1780    1847    1997    2041    2126 
   Size:        296      89     123      36      67     150      44      85

Cuts at:   2126    2486    2612    2714    3093    3187    3200    3296    3320 
   Size:        360     126     102     379      94      13      96      24

Cuts at:   3320    3474 
   Size:        154

  Fragments arranged by size: 

                 379     360     342     296     282     159     154     150
                 136     132     126     123     102      96      94      89
                  85      85      67      61      44      39      36      24
                  13
 
BspEI T'CCGG_A

Cuts at:      0     258    3474 
   Size:        258    3216
 
BspHI T'CATG_A

Cuts at:      0    1718    2643    3474 
   Size:       1718     925     831

  Fragments arranged by size: 

                1718     925     831
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    2597    2697    2905    3474 
   Size:       2597     100     208     569

  Fragments arranged by size: 

                2597     569     208     100
 
BsrBI CCG'CTC

Cuts at:      0     801    2298    3474 
   Size:        801    1497    1176

  Fragments arranged by size: 

                1497    1176     801
 
BsrDI GCAATG_nn'

Cuts at:      0    1443    2240    2756    2907    3474 
   Size:       1443     797     516     151     567

  Fragments arranged by size: 

                1443     797     567     516     151
 
BsrFI r'CCGG_y

Cuts at:      0    1244    1261    1539    2518    2996    3474 
   Size:       1244      17     278     979     478     478

  Fragments arranged by size: 

                1244     979     478     478     278      17
 
BsrGI T'GTAC_A

Cuts at:      0    1460    3474 
   Size:       1460    2014
 
BssSI C'ACGA_G

Cuts at:      0    1781    3474 
   Size:       1781    1693
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      81    1592    3474 
   Size:         81    1511    1882

  Fragments arranged by size: 

                1882    1511      81
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1167    1577    2604    3474 
   Size:       1167     410    1027     870

  Fragments arranged by size: 

                1167    1027     870     410
 
BstYI r'GATC_y

Cuts at:      0    1196    1793    1808    1868    2109    3474 
   Size:       1196     597      15      60     241    1365

  Fragments arranged by size: 

                1365    1196     597     241      60      15
 
Bsu36I CC'TnA_GG

Cuts at:      0     407     821    3474 
   Size:        407     414    2653

  Fragments arranged by size: 

                2653     414     407
 
BtgI C'CryG_G

Cuts at:      0     190    1221    1802    1872    2213    2222    2363    2490 
   Size:        190    1031     581      70     341       9     141     127

Cuts at:   2490    2715    3474 
   Size:        225     759

  Fragments arranged by size: 

                1031     759     581     341     225     190     141     127
                  70       9
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1955    3005    3474 
   Size:       1955    1050     469

  Fragments arranged by size: 

                1955    1050     469
 
BtsI GCAGTG_nn'

Cuts at:      0     544    1076    1859    3474 
   Size:        544     532     783    1615

  Fragments arranged by size: 

                1615     783     544     532
 
DraIII CAC_nnn'GTG

Cuts at:      0    1992    2799    2930    3144    3474 
   Size:       1992     807     131     214     330

  Fragments arranged by size: 

                1992     807     330     214     131
 
EaeI y'GGCC_r

Cuts at:      0       5     232     242     541     808    1242    1664    2354 
   Size:          5     227      10     299     267     434     422     690

Cuts at:   2354    2981    3417    3474 
   Size:        627     436      57

  Fragments arranged by size: 

                 690     627     436     434     422     299     267     227
                  57      10       5
 
EagI C'GGCC_G

Cuts at:      0       5     242    1242    3474 
   Size:          5     237    1000    2232

  Fragments arranged by size: 

                2232    1000     237       5
 
EarI CTCTTCn'nnn_

Cuts at:      0     496     995    3374    3392    3474 
   Size:        496     499    2379      18      82

  Fragments arranged by size: 

                2379     499     496      82      18
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     953    3474 
   Size:        953    2521
 
EcoICRI GAG'CTC

Cuts at:      0    1530    1778    2610    3474 
   Size:       1530     248     832     864

  Fragments arranged by size: 

                1530     864     832     248
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      83     556    1036    1374    1416    1624    2402    2565 
   Size:         83     473     480     338      42     208     778     163

Cuts at:   2565    2626    3474 
   Size:         61     848

  Fragments arranged by size: 

                 848     778     480     473     338     208     163      83
                  61      42
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1969    3474 
   Size:       1969    1505
 
EcoO109I rG'GnC_Cy

Cuts at:      0     165     304     335     506     831    1319    2151    2163 
   Size:        165     139      31     171     325     488     832      12

Cuts at:   2163    3133    3204    3474 
   Size:        970      71     270

  Fragments arranged by size: 

                 970     832     488     325     270     171     165     139
                  71      31      12
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     342    2280    3474 
   Size:        342    1938    1194

  Fragments arranged by size: 

                1938    1194     342
 
FspI TGC'GCA

Cuts at:      0    1960    3474 
   Size:       1960    1514
 
HaeII r_GCGC'y

Cuts at:      0      22      72     601     756     990    2062    2689    2891 
   Size:         22      50     529     155     234    1072     627     202

Cuts at:   2891    3111    3474 
   Size:        220     363

  Fragments arranged by size: 

                1072     627     529     363     234     220     202     155
                  50      22
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     849     881    2101    2133    2383    2415    3474 
   Size:        849      32    1220      32     250      32    1059

  Fragments arranged by size: 

                1220    1059     849     250      32      32      32
 
HincII GTy'rAC

Cuts at:      0    2538    2907    3474 
   Size:       2538     369     567

  Fragments arranged by size: 

                2538     567     369
 
KasI G'GCGC_C

Cuts at:      0      18      68     597     752    3107    3474 
   Size:         18      50     529     155    2355     367

  Fragments arranged by size: 

                2355     529     367     155      50      18
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1097    2978    3221    3317    3474 
   Size:       1097    1881     243      96     157

  Fragments arranged by size: 

                1881    1097     243     157      96
 
MscI TGG'CCA

Cuts at:      0     543     810    1666    2983    3419    3474 
   Size:        543     267     856    1317     436      55

  Fragments arranged by size: 

                1317     856     543     436     267      55
 
MslI CAynn'nnrTG

Cuts at:      0    1165    1516    1723    2648    2698    2776    2983    3262 
   Size:       1165     351     207     925      50      78     207     279

Cuts at:   3262    3474 
   Size:        212

  Fragments arranged by size: 

                1165     925     351     279     212     207     207      78
                  50
 
MspA1I CmG'CkG

Cuts at:      0      32     192     891    2349    2492    2717    3226    3360 
   Size:         32     160     699    1458     143     225     509     134

Cuts at:   3360    3474 
   Size:        114

  Fragments arranged by size: 

                1458     699     509     225     160     143     134     114
                  32
 
NaeI GCC'GGC

Cuts at:      0    1246    3474 
   Size:       1246    2228
 
NarI GG'CG_CC

Cuts at:      0      19      69     598     753    3108    3474 
   Size:         19      50     529     155    2355     366

  Fragments arranged by size: 

                2355     529     366     155      50      19
 
NcoI C'CATG_G

Cuts at:      0    1802    1872    2363    3474 
   Size:       1802      70     491    1111

  Fragments arranged by size: 

                1802    1111     491      70
 
NgoMIV G'CCGG_C

Cuts at:      0    1244    3474 
   Size:       1244    2230
 
NspI r_CATG'y

Cuts at:      0    1257    1599    1710    3474 
   Size:       1257     342     111    1764

  Fragments arranged by size: 

                1764    1257     342     111
 
PasI CC'CwG_GG

Cuts at:      0    1377    1822    3200    3474 
   Size:       1377     445    1378     274

  Fragments arranged by size: 

                1378    1377     445     274
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1227    3474 
   Size:       1227    2247
 
PfoI T'CCnGG_A

Cuts at:      0     624    2105    2969    3474 
   Size:        624    1481     864     505

  Fragments arranged by size: 

                1481     864     624     505
 
PmlI CAC'GTG

Cuts at:      0    1954    3474 
   Size:       1954    1520
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1834    1866    3474 
   Size:       1834      32    1608

  Fragments arranged by size: 

                1834    1608      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     335    1319    2151    2163    3474 
   Size:        335     984     832      12    1311

  Fragments arranged by size: 

                1311     984     832     335      12
 
PspXI vC'TCGA_Gb

Cuts at:      0     112    3474 
   Size:        112    3362
 
PstI C_TGCA'G

Cuts at:      0    3056    3474 
   Size:       3056     418
 
PvuII CAG'CTG

Cuts at:      0      32    2349    3360    3474 
   Size:         32    2317    1011     114

  Fragments arranged by size: 

                2317    1011     114      32
 
RsrII CG'GwC_CG

Cuts at:      0    2561    3474 
   Size:       2561     913
 
SacI G_AGCT'C

Cuts at:      0    1532    1780    2612    3474 
   Size:       1532     248     832     862

  Fragments arranged by size: 

                1532     862     832     248
 
SacII CC_GC'GG

Cuts at:      0     193    2493    2718    3474 
   Size:        193    2300     225     756

  Fragments arranged by size: 

                2300     756     225     193
 
SanDI GG'GwC_CC

Cuts at:      0     335    2151    2163    3474 
   Size:        335    1816      12    1311

  Fragments arranged by size: 

                1816    1311     335      12
 
SexAI A'CCwGG_T

Cuts at:      0    1988    3474 
   Size:       1988    1486
 
SfcI C'TryA_G

Cuts at:      0    1635    3052    3474 
   Size:       1635    1417     422

  Fragments arranged by size: 

                1635    1417     422
 
SfoI GGC'GCC

Cuts at:      0      20      70     599     754    3109    3474 
   Size:         20      50     529     155    2355     365

  Fragments arranged by size: 

                2355     529     365     155      50      20
 
SgrAI Cr'CCGG_yG

Cuts at:      0    2518    3474 
   Size:       2518     956
 
SmaI CCC'GGG

Cuts at:      0     148    2377    2843    3474 
   Size:        148    2229     466     631

  Fragments arranged by size: 

                2229     631     466     148
 
SmlI C'TyrA_G

Cuts at:      0     112    1789    3474 
   Size:        112    1677    1685

  Fragments arranged by size: 

                1685    1677     112
 
SphI G_CATG'C

Cuts at:      0    1599    3474 
   Size:       1599    1875
 
StuI AGG'CCT

Cuts at:      0    1787    3474 
   Size:       1787    1687
 
StyI C'CwwG_G

Cuts at:      0     308     317     326     374     392     425     440     473 
   Size:        308       9       9      48      18      33      15      33

Cuts at:    473    1046    1802    1872    2363    3129    3308    3474 
   Size:        573     756      70     491     766     179     166

  Fragments arranged by size: 

                 766     756     573     491     308     179     166      70
                  48      33      33      18      15       9       9
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     103     387     453     486     697    1072    1390    1443 
   Size:        103     284      66      33     211     375     318      53

Cuts at:   1443    2494    3298    3474 
   Size:       1051     804     176

  Fragments arranged by size: 

                1051     804     375     318     284     211     176     103
                  66      53      33
 
TatI w'GTAC_w

Cuts at:      0     743    1460    3474 
   Size:        743     717    2014

  Fragments arranged by size: 

                2014     743     717
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1111    1143    1938    1970    3132    3164    3474 
   Size:       1111      32     795      32    1162      32     310

  Fragments arranged by size: 

                1162    1111     795     310      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    2395    3474 
   Size:       2395    1079
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     324    1422    1674    3474 
   Size:        324    1098     252    1800

  Fragments arranged by size: 

                1800    1098     324     252
 
XhoI C'TCGA_G

Cuts at:      0     112    3474 
   Size:        112    3362
 
XmaI C'CCGG_G

Cuts at:      0     146    2375    2841    3474 
   Size:        146    2229     466     633

  Fragments arranged by size: 

                2229     633     466     146
 
XmnI GAAnn'nnTTC

Cuts at:      0    2263    3474 
   Size:       2263    1211
 

 Enzymes that do cut:

     AarI     AccI     AcuI   AflIII     AgeI     AleI     AlfI     AloI
    AlwNI    ApaLI     AseI     AvaI    AvrII    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
    BssSI   BstAPI    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI
   DraIII     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I     FalI     FspI    HaeII    Hin4I   HincII     KasI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI
     PasI    PflMI     PfoI     PmlI     PpiI    PpuMI    PspXI     PstI
    PvuII    RsrII     SacI    SacII    SanDI    SexAI     SfcI     SfoI
    SgrAI     SmaI     SmlI     SphI     StuI     StyI    TaqII     TatI
     TstI  Tth111I     XcmI     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII     AhdI     ApaI     ApoI
     AscI    AsiSI     BaeI     BclI    BfrBI     BglI     BmtI     BplI
    BsaBI    BsiWI    BsmBI   BssHII    BstBI   BstEII  BstZ17I     ClaI
    CspCI     DraI     DrdI    EcoRI    EcoRV     FseI    FspAI  HindIII
     HpaI     KpnI     MfeI     MluI     NdeI     NheI     NotI     NruI
     NsiI     PacI     PciI     PmeI    PshAI     PsiI   PspOMI     PsrI
     PvuI     SalI     SapI     SbfI     ScaI     SfiI     SgfI    SnaBI
     SpeI     SrfI     SspI     SwaI     XbaI     ZraI
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