Restriction map for pF1KB5093
##################################
# NO CUT ENZYMES (   88 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AgeI        
AhdI        AscI        Asp718I     AspEI       AspI        
AviII       BalI        BamHI       BbrPI       BcgI        
BsaAI       BsaWI       BseAI       BsiWI       BsmBI       
BsmI        BspDI       BspEI       BspHI       BspLU11I    
BsrBI       BsrFI       BssHII      BssSI       Bst1107I    
BstBI       BstEII      BstPI       BstXI       BstZ17I     
Cfr10I      ClaI        CpoI        DrdI        DsaI        
Eam1105I    EcoO65I     EcoRI       FseI        FspI        
HgaI        KpnI        KspI        MluI        MluNI       
MroI        MscI        NaeI        NcoI        NgoMIV      
NotI        NruI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PvuI        RcaI        RsrII       SacI        
SacII       SalI        SbfI        ScaI        SfiI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SwaI        Tth111I     XcmI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AclI         2124  AACGTT               AA^CGTT
AflII         819  CTTAAG               C^TTAAG
AflIII       2451  ACGTGT               A^C[GA][CT]GT
Alw44I        508  GTGCAC               G^TGCAC
AlwI         2470  GGATC                GGATC(4/5)
Aor51HI       180  AGCGCT               AGC^GCT
ApaLI         508  GTGCAC               G^TGCAC
AspHI         508  GTGCAC               G[AT]GC[AT]^C
AvrII        2010  CCTAGG               C^CTAGG
BbsI         2428  GAAGAC               GAAGAC(2/6)
BbvCI         516  CCTCAGC              CCTCAGC(-5/-2)
BciVI         572  GTATCC               GTATCC(6/5)
BclI         2312  TGATCA               T^GATCA
BfrI          819  CTTAAG               C^TTAAG
BlnI         2010  CCTAGG               C^CTAGG
BlpI          162  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      162  GCTCAGC              GC^T[ACGT]AGC
BpuAI        2428  GAAGAC               GAAGAC(2/6)
BsaBI         805  GATGGTAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI        1881  CTCCTC               GAGGAG(10/8)
BsiHKAI       508  GTGCAC               G[AT]GC[AT]^C
BsmFI         430  GTCCC                GGGAC(10/14)
BspMI        1667  ACCTGC               ACCTGC(4/8)
BsrDI        2372  CATTGC               GCAATG(2/0)
CelII         162  GCTCAGC              GC^T[ACGT]AGC
DraII        2204  AGGACCT              [GA]G^G[ACGT]CC[CT]
DraIII       1474  CACAGTGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eco47III      180  AGCGCT               AGC^GCT
EcoO109I     2204  AGGACCT              [GA]G^G[ACGT]CC[CT]
EcoT22I       224  ATGCAT               ATGCA^T
FbaI         2312  TGATCA               T^GATCA
HindIII      1929  AAGCTT               A^AGCTT
HpaI         1369  GTTAAC               GTT^AAC
MamI          805  GATGGTAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI          832  CATTTGTATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeI         1394  CATATG               CA^TATG
NheI         1911  GCTAGC               G^CTAGC
NsiI          224  ATGCAT               ATGCA^T
PpuMI        2204  AGGACCT              [GA]G^G[AT]CC[CT]
Psp1406I     2124  AACGTT               AA^CGTT
PstI           96  CTGCAG               CTGCA^G
SexAI        2762  ACCAGGT              A^CC[AT]GGT


###################################
# TWO CUT ENZYMES (   55 enzymes) #
###################################

AccII          69  CGCG                 CG^CG
               87  CGCG                
AcyI           72  GGCGCC               G[GA]^CG[CT]C
               90  GGCGCC              
ApaI            6  GGGCCC               GGGCC^C
               58  GGGCCC              
AseI         1905  ATTAAT               AT^TAAT
             2157  ATTAAT              
AsnI         1905  ATTAAT               AT^TAAT
             2157  ATTAAT              
Asp700I       367  GAAGCCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2584  GAATCGTTTC          
AvaI          154  CCCGAG               C^[CT]CG[GA]G
              433  CCCGAG              
BbeI           72  GGCGCC               GGCGC^C
               90  GGCGCC              
BglI          153  GCCCGAGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1996  GCCCTTTTGGC         
BglII         563  AGATCT               A^GATCT
             1182  AGATCT              
BsaHI          72  GGCGCC               G[GA]^CG[CT]C
               90  GGCGCC              
BsaI          682  GAGACC               GGTCTC(1/5)
             1067  GAGACC              
BsgI          925  GTGCAG               GTGCAG(16/14)
             2767  GTGCAG              
BsoBI         154  CCCGAG               C^[CT]CG[GA]G
              433  CCCGAG              
Bsp1407I     1564  TGTACA               T^GTACA
             1699  TGTACA              
BsrGI        1564  TGTACA               T^GTACA
             1699  TGTACA              
BstAPI        393  GCAGTCTTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1844  GCAGTGGCTGC         
BstUI          69  CGCG                 CG^CG
               87  CGCG                
BstYI         563  AGATCT               [GA]^GATC[CT]
             1182  AGATCT              
DraI         1443  TTTAAA               TTT^AAA
             2422  TTTAAA              
EagI           53  CGGCCG               C^GGCCG
              146  CGGCCG              
EarI          250  GAAGAG               CTCTTC(1/4)
             1962  GAAGAG              
EclXI          53  CGGCCG               C^GGCCG
              146  CGGCCG              
Eco52I         53  CGGCCG               C^GGCCG
              146  CGGCCG              
EcoNI        1285  CCTACGGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1570  CCTTCAACAGG         
EcoRV         187  GATATC               GAT^ATC
             1164  GATATC              
EcoT14I      2010  CCTAGG               C^C[AT][AT]GG
             2496  CCAAGG              
FauI            9  CCCGC                CCCGC(4/6)
              265  GCGGG               
Hin1I          72  GGCGCC               G[GA]^CG[CT]C
               90  GGCGCC              
HincII        650  GTCAAC               GT[CT]^[GA]AC
             1369  GTTAAC              
HindII        650  GTCAAC               GT[CT]^[GA]AC
             1369  GTTAAC              
KasI           72  GGCGCC               G^GCGCC
               90  GGCGCC              
Ksp632I       250  GAAGAG               CTCTTC(1/4)
             1962  GAAGAG              
MaeII        2125  ACGT                 A^CGT
             2451  ACGT                
MfeI          259  CAATTG               C^AATTG
             2265  CAATTG              
MflI          563  AGATCT               [GA]^GATC[CT]
             1182  AGATCT              
MunI          259  CAATTG               C^AATTG
             2265  CAATTG              
MvnI           69  CGCG                 CG^CG
               87  CGCG                
NarI           72  GGCGCC               GG^CGCC
               90  GGCGCC              
NspI         2241  GCATGT               [GA]CATG^[CT]
             2537  GCATGT              
PleI         1429  GACTC                GAGTC(4/5)
              648  GAGTC               
PshBI        1905  ATTAAT               AT^TAAT
             2157  ATTAAT              
PvuII          99  CAGCTG               CAG^CTG
             2637  CAGCTG              
SapI          250  GAAGAGC              GCTCTTC(1/4)
             1962  GAAGAGC             
SfoI           72  GGCGCC               GGC^GCC
               90  GGCGCC              
SmlI          567  CTCAAG               C^T[CT][GA]AG
              819  CTTAAG              
SspBI        1564  TGTACA               T^GTACA
             1699  TGTACA              
SspI         1598  AATATT               AAT^ATT
             2057  AATATT              
StyI         2010  CCTAGG               C^C[AT][AT]GG
             2496  CCAAGG              
TaqI          375  TCGA                 T^CGA
             1922  TCGA                
Tsp45I       1427  GTGAC                ^GT[GC]AC
             1516  GTCAC               
TthHB8I       375  TCGA                 T^CGA
             1922  TCGA                
XbaI          246  TCTAGA               T^CTAGA
             1179  TCTAGA              
XhoII         563  AGATCT               [GA]^GATC[CT]
             1182  AGATCT              
XmnI          367  GAAGCCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2584  GAATCGTTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   73 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwNI       
AocI        ApoI        AvaII       BanI        BanII       
BbvI        BcnI        BfaI        BmyI        BpmI        
BsaJI       BsiEI       BsiYI       BslI        BsmAI       
Bsp1286I    BsrI        BssKI       BstF5I      BstNI       
Bsu36I      Cac8I       CfoI        Cfr13I      DdeI        
DpnII       EaeI        Eco81I      EcoRII      Fnu4HI      
FokI        HaeII       HaeIII      HapII       HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
MaeI        MaeIII      MboI        MboII       MnlI        
MseI        MspA1I      MspI        MvaI        MwoI        
NciI        NdeII       NlaIII      NlaIV       PflMI       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        StuI        TfiI        Tru9I       TseI        
Tsp509I     TspRI       Van91I      
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