Restriction map for pF1KB5093
 (Linear) (Six-base) MAPSORT of: KIBB5093.seq  Check: 6717  from: 1  to: 2853



KIBB5093.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:26  ..

AclI AA'CG_TT

Cuts at:      0    2125    2853 
   Size:       2125     728
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2464    2853 
   Size:       2464     389
 
AfeI AGC'GCT

Cuts at:      0     182    2853 
   Size:        182    2671
 
AflII C'TTAA_G

Cuts at:      0     819    2853 
   Size:        819    2034
 
AflIII A'CryG_T

Cuts at:      0    2451    2853 
   Size:       2451     402
 
AlwNI CAG_nnn'CTG

Cuts at:      0     543     952    1850    2853 
   Size:        543     409     898    1003

  Fragments arranged by size: 

                1003     898     543     409
 
ApaI G_GGCC'C

Cuts at:      0      10      62    2853 
   Size:         10      52    2791

  Fragments arranged by size: 

                2791      52      10
 
ApaLI G'TGCA_C

Cuts at:      0     508    2853 
   Size:        508    2345
 
ApoI r'AATT_y

Cuts at:      0     715    1106    1208    1440    1819    2853 
   Size:        715     391     102     232     379    1034

  Fragments arranged by size: 

                1034     715     391     379     232     102
 
AseI AT'TA_AT

Cuts at:      0    1906    2158    2853 
   Size:       1906     252     695

  Fragments arranged by size: 

                1906     695     252
 
AvaI C'yCGr_G

Cuts at:      0     154     433    2853 
   Size:        154     279    2420

  Fragments arranged by size: 

                2420     279     154
 
AvrII C'CTAG_G

Cuts at:      0    2010    2853 
   Size:       2010     843
 
BanI G'GyrC_C

Cuts at:      0      72      90     501    1147    2853 
   Size:         72      18     411     646    1706

  Fragments arranged by size: 

                1706     646     411      72      18
 
BanII G_rGCy'C

Cuts at:      0      10      62     164    2853 
   Size:         10      52     102    2689

  Fragments arranged by size: 

                2689     102      52      10
 
BbeI G_GCGC'C

Cuts at:      0      76      94    2853 
   Size:         76      18    2759

  Fragments arranged by size: 

                2759      76      18
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    2435    2853 
   Size:       2435     418
 
BbvCI CC'TCA_GC

Cuts at:      0     517    2853 
   Size:        517    2336
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     583    2853 
   Size:        583    2270
 
BclI T'GATC_A

Cuts at:      0    2312    2853 
   Size:       2312     541
 
BfrBI ATG'CAT

Cuts at:      0     226    2853 
   Size:        226    2627
 
BglI GCCn_nnn'nGGC

Cuts at:      0     159    2002    2853 
   Size:        159    1843     851

  Fragments arranged by size: 

                1843     851     159
 
BglII A'GATC_T

Cuts at:      0     563    1182    2853 
   Size:        563     619    1671

  Fragments arranged by size: 

                1671     619     563
 
BlpI GC'TnA_GC

Cuts at:      0     163    2853 
   Size:        163    2690
 
Bme1580I G_kGCm'C

Cuts at:      0      10      62     155     512    2853 
   Size:         10      52      93     357    2341

  Fragments arranged by size: 

                2341     357      93      52      10
 
BmgBI CAC'GTC

Cuts at:      0    2452    2853 
   Size:       2452     401
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     356    1615    2853 
   Size:        356    1259    1238

  Fragments arranged by size: 

                1259    1238     356
 
BmtI G_CTAG'C

Cuts at:      0    1915    2853 
   Size:       1915     938
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2226    2853 
   Size:       2226     627
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     319     700    1450    2853 
   Size:        319     381     750    1403

  Fragments arranged by size: 

                1403     750     381     319
 
Bpu10I CC'TnA_GC

Cuts at:      0     517    1033    1350    2853 
   Size:        517     516     317    1503

  Fragments arranged by size: 

                1503     517     516     317
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     552    2853 
   Size:        552    2301
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     676    1061    2853 
   Size:        676     385    1792

  Fragments arranged by size: 

                1792     676     385
 
BsaBI GATnn'nnATC

Cuts at:      0     809    2853 
   Size:        809    2044
 
BsaHI Gr'CG_yC

Cuts at:      0      73      91    2853 
   Size:         73      18    2762

  Fragments arranged by size: 

                2762      73      18
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1872    2853 
   Size:       1872     981
 
BseYI C'CCAG_C

Cuts at:      0      61    1861    2853 
   Size:         61    1800     992

  Fragments arranged by size: 

                1800     992      61
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     946    2788    2853 
   Size:        946    1842      65

  Fragments arranged by size: 

                1842     946      65
 
BsiEI CG_ry'CG

Cuts at:      0      56     114     149    2853 
   Size:         56      58      35    2704

  Fragments arranged by size: 

                2704      58      56      35
 
BsiHKAI G_wGCw'C

Cuts at:      0     512    2853 
   Size:        512    2341
 
Bsp1286I G_dGCh'C

Cuts at:      0      10      62     155     164     512    2853 
   Size:         10      52      93       9     348    2341

  Fragments arranged by size: 

                2341     348      93      52      10       9
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1676    2853 
   Size:       1676    1177
 
BsrDI GCAATG_nn'

Cuts at:      0    2371    2853 
   Size:       2371     482
 
BsrGI T'GTAC_A

Cuts at:      0    1564    1699    2853 
   Size:       1564     135    1154

  Fragments arranged by size: 

                1564    1154     135
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     399    1850    2853 
   Size:        399    1451    1003

  Fragments arranged by size: 

                1451    1003     399
 
BstYI r'GATC_y

Cuts at:      0     563    1182    2853 
   Size:        563     619    1671

  Fragments arranged by size: 

                1671     619     563
 
Bsu36I CC'TnA_GG

Cuts at:      0     722    1375    2675    2853 
   Size:        722     653    1300     178

  Fragments arranged by size: 

                1300     722     653     178
 
BtsI GCAGTG_nn'

Cuts at:      0    1705    1851    2315    2660    2853 
   Size:       1705     146     464     345     193

  Fragments arranged by size: 

                1705     464     345     193     146
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    2448    2483    2853 
   Size:       2448      35     370

  Fragments arranged by size: 

                2448     370      35
 
DraI TTT'AAA

Cuts at:      0    1445    2424    2853 
   Size:       1445     979     429

  Fragments arranged by size: 

                1445     979     429
 
DraIII CAC_nnn'GTG

Cuts at:      0    1479    2853 
   Size:       1479    1374
 
EaeI y'GGCC_r

Cuts at:      0      53     146    2162    2853 
   Size:         53      93    2016     691

  Fragments arranged by size: 

                2016     691      93      53
 
EagI C'GGCC_G

Cuts at:      0      53     146    2853 
   Size:         53      93    2707

  Fragments arranged by size: 

                2707      93      53
 
EarI CTCTTCn'nnn_

Cuts at:      0     245    1957    2853 
   Size:        245    1712     896

  Fragments arranged by size: 

                1712     896     245
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     319     700    1450    2464    2853 
   Size:        319     381     750    1014     389

  Fragments arranged by size: 

                1014     750     389     381     319
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1289    1574    2853 
   Size:       1289     285    1279

  Fragments arranged by size: 

                1289    1279     285
 
EcoO109I rG'GnC_Cy

Cuts at:      0    2205    2853 
   Size:       2205     648
 
EcoRV GAT'ATC

Cuts at:      0     189    1166    2853 
   Size:        189     977    1687

  Fragments arranged by size: 

                1687     977     189
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     989    2853 
   Size:        989    1864
 
HaeII r_GCGC'y

Cuts at:      0      76      94     170     184     544    2853 
   Size:         76      18      76      14     360    2309

  Fragments arranged by size: 

                2309     360      76      76      18      14
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     537     569    1167    1199    1608    1640    1783    1815 
   Size:        537      32     598      32     409      32     143      32

Cuts at:   1815    1956    1988    2853 
   Size:        141      32     865

  Fragments arranged by size: 

                 865     598     537     409     143     141      32      32
                  32      32      32
 
HincII GTy'rAC

Cuts at:      0     652    1371    2853 
   Size:        652     719    1482

  Fragments arranged by size: 

                1482     719     652
 
HindIII A'AGCT_T

Cuts at:      0    1929    2853 
   Size:       1929     924
 
HpaI GTT'AAC

Cuts at:      0    1371    2853 
   Size:       1371    1482
 
KasI G'GCGC_C

Cuts at:      0      72      90    2853 
   Size:         72      18    2763

  Fragments arranged by size: 

                2763      72      18
 
MfeI C'AATT_G

Cuts at:      0     259    2265    2853 
   Size:        259    2006     588

  Fragments arranged by size: 

                2006     588     259
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    2714    2853 
   Size:       2714     139
 
MslI CAynn'nnrTG

Cuts at:      0     836    2853 
   Size:        836    2017
 
MspA1I CmG'CkG

Cuts at:      0      65     101    2639    2853 
   Size:         65      36    2538     214

  Fragments arranged by size: 

                2538     214      65      36
 
NarI GG'CG_CC

Cuts at:      0      73      91    2853 
   Size:         73      18    2762

  Fragments arranged by size: 

                2762      73      18
 
NdeI CA'TA_TG

Cuts at:      0    1395    2853 
   Size:       1395    1458
 
NheI G'CTAG_C

Cuts at:      0    1911    2853 
   Size:       1911     942
 
NsiI A_TGCA'T

Cuts at:      0     228    2853 
   Size:        228    2625
 
NspI r_CATG'y

Cuts at:      0    2245    2541    2853 
   Size:       2245     296     312

  Fragments arranged by size: 

                2245     312     296
 
PasI CC'CwG_GG

Cuts at:      0      36    2853 
   Size:         36    2817
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     183    1098    1479    2853 
   Size:        183     915     381    1374

  Fragments arranged by size: 

                1374     915     381     183
 
PfoI T'CCnGG_A

Cuts at:      0     130    1466    2853 
   Size:        130    1336    1387

  Fragments arranged by size: 

                1387    1336     130
 
PpuMI rG'GwC_Cy

Cuts at:      0    2205    2853 
   Size:       2205     648
 
PsiI TTA'TAA

Cuts at:      0     701    2853 
   Size:        701    2152
 
PspOMI G'GGCC_C

Cuts at:      0       6      58    2853 
   Size:          6      52    2795

  Fragments arranged by size: 

                2795      52       6
 
PstI C_TGCA'G

Cuts at:      0     100    2853 
   Size:        100    2753
 
PvuII CAG'CTG

Cuts at:      0     101    2639    2853 
   Size:        101    2538     214

  Fragments arranged by size: 

                2538     214     101
 
SapI GCTCTTCn'nnn_

Cuts at:      0     245    1957    2853 
   Size:        245    1712     896

  Fragments arranged by size: 

                1712     896     245
 
SexAI A'CCwGG_T

Cuts at:      0    2762    2853 
   Size:       2762      91
 
SfcI C'TryA_G

Cuts at:      0      96     535    2018    2090    2853 
   Size:         96     439    1483      72     763

  Fragments arranged by size: 

                1483     763     439      96      72
 
SfoI GGC'GCC

Cuts at:      0      74      92    2853 
   Size:         74      18    2761

  Fragments arranged by size: 

                2761      74      18
 
SmlI C'TyrA_G

Cuts at:      0     567     819    2853 
   Size:        567     252    2034

  Fragments arranged by size: 

                2034     567     252
 
SspI AAT'ATT

Cuts at:      0    1600    2059    2853 
   Size:       1600     459     794

  Fragments arranged by size: 

                1600     794     459
 
StuI AGG'CCT

Cuts at:      0     298    1160    1295    2773    2853 
   Size:        298     862     135    1478      80

  Fragments arranged by size: 

                1478     862     298     135      80
 
StyI C'CwwG_G

Cuts at:      0    2010    2496    2853 
   Size:       2010     486     357

  Fragments arranged by size: 

                2010     486     357
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     190     305    2853 
   Size:        190     115    2548

  Fragments arranged by size: 

                2548     190     115
 
TatI w'GTAC_w

Cuts at:      0    1564    1699    2540    2853 
   Size:       1564     135     841     313

  Fragments arranged by size: 

                1564     841     313     135
 
XbaI T'CTAG_A

Cuts at:      0     246    1179    2853 
   Size:        246     933    1674

  Fragments arranged by size: 

                1674     933     246
 
XmnI GAAnn'nnTTC

Cuts at:      0     371    2588    2853 
   Size:        371    2217     265

  Fragments arranged by size: 

                2217     371     265
 

 Enzymes that do cut:

     AclI     AcuI     AfeI    AflII   AflIII    AlwNI     ApaI    ApaLI
     ApoI     AseI     AvaI    AvrII     BanI    BanII     BbeI     BbsI
    BbvCI    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaBI    BsaHI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I
    BspMI    BsrDI    BsrGI   BstAPI    BstYI   Bsu36I     BtsI    CspCI
     DraI   DraIII     EaeI     EagI     EarI  Eco57MI    EcoNI EcoO109I
    EcoRV     FalI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI
     MfeI     MmeI     MslI   MspA1I     NarI     NdeI     NheI     NsiI
     NspI     PasI    PflMI     PfoI    PpuMI     PsiI   PspOMI     PstI
    PvuII     SapI    SexAI     SfcI     SfoI     SmlI     SspI     StuI
     StyI    TaqII     TatI     XbaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AgeI     AhdI     AleI     AlfI
     AloI     AscI    AsiSI     BaeI    BamHI     BcgI    BsaAI    BsaWI
    BsaXI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrFI
   BssHII    BssSI    BstBI   BstEII    BstXI  BstZ17I     BtgI    BtgZI
     ClaI     DrdI     EciI  EcoICRI    EcoRI     FseI     FspI    FspAI
     KpnI     MluI     MscI     NaeI     NcoI   NgoMIV     NotI     NruI
     PacI     PciI     PmeI     PmlI     PpiI    PshAI    PspXI     PsrI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SbfI     ScaI
     SfiI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SwaI     TstI  Tth111I     XcmI     XhoI     XmaI     ZraI
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