Restriction map for pF1KB5062
##################################
# NO CUT ENZYMES (  124 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AflII       AgeI        AhdI        Alw44I      
AlwNI       Aor51HI     ApaI        ApaLI       AscI        
Asp718I     AspEI       AspI        AvaI        AviII       
AvrII       BamHI       BanII       BbeI        BbvCI       
BcgI        BclI        BfrI        BglI        BglII       
BlnI        BlpI        Bpu1102I    BsaBI       BsaHI       
BsaWI       BseAI       BsgI        BsiEI       BsiWI       
BsmBI       BsoBI       Bsp1407I    BspEI       BsrBI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstZ17I     
CelII       CfoI        Cfr10I      CpoI        DraIII      
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoNI       EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        FseI        FspI        HaeII       
HgaI        HhaI        Hin1I       HinP1I      HindIII     
HpaI        KasI        KpnI        KspI        MamI        
MluI        MroI        NaeI        NarI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PmeI        PshAI       Psp1406I    PvuI        RsrII       
SacI        SacII       SalI        SbfI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SphI        Sse8387I    SspBI       SwaI        Tth111I     
XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   57 enzymes) #
###################################

AcsI         1753  GAATTT               [GA]^AATT[CT]
AflIII       1819  ACATGT               A^C[GA][CT]GT
AocI          779  CCTCAGG              CC^T[ACGT]AGG
ApoI         1753  GAATTT               [GA]^AATT[CT]
AseI          892  ATTAAT               AT^TAAT
AsnI          892  ATTAAT               AT^TAAT
Asp700I      1356  GAAGCTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI         329  GAGCAC               G[AT]GC[AT]^C
AvaII         238  GGACC                G^G[AT]CC
BalI         1574  TGGCCA               TGG^CCA
BanI         1228  GGTGCC               G^G[CT][GA]CC
BbrPI        1888  CACGTG               CAC^GTG
BcnI         1058  CCGGG                CC^[GC]GG
BfaI          581  CTAG                 C^TAG
BsaI          220  GGTCTC               GGTCTC(1/5)
BsiHKAI       329  GAGCAC               G[AT]GC[AT]^C
BsmI         1715  GCATTC               GAATGC(1/-1)
BspDI        1855  ATCGAT               AT^CGAT
BspLU11I     1819  ACATGT               A^CATGT
BsrDI        1680  CATTGC               GCAATG(2/0)
BstYI         291  AGATCC               [GA]^GATC[CT]
Bsu36I        779  CCTCAGG              CC^T[ACGT]AGG
ClaI         1855  ATCGAT               AT^CGAT
DraI          937  TTTAAA               TTT^AAA
DraII         237  GGGACCC              [GA]G^G[ACGT]CC[CT]
EaeI         1574  TGGCCA               [CT]^GGCC[GA]
Eco81I        779  CCTCAGG              CC^T[ACGT]AGG
EcoO109I      237  GGGACCC              [GA]G^G[ACGT]CC[CT]
FauI           61  CCCGC                CCCGC(4/6)
HapII        1058  CCGG                 C^CGG
HincII       1009  GTCAAC               GT[CT]^[GA]AC
HindII       1009  GTCAAC               GT[CT]^[GA]AC
HpaII        1058  CCGG                 C^CGG
MaeI          581  CTAG                 C^TAG
MfeI         1925  CAATTG               C^AATTG
MflI          291  AGATCC               [GA]^GATC[CT]
MluNI        1574  TGGCCA               TGG^CCA
MscI         1574  TGGCCA               TGG^CCA
MspI         1058  CCGG                 C^CGG
MunI         1925  CAATTG               C^AATTG
NciI         1058  CCGGG                CC^[GC]GG
NcoI         1565  CCATGG               C^CATGG
NspI         1819  ACATGT               [GA]CATG^[CT]
PflMI        1720  CCACGATATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI        1888  CACGTG               CAC^GTG
PmlI         1888  CACGTG               CAC^GTG
PpuMI         237  GGGACCC              [GA]G^G[AT]CC[CT]
PshBI         892  ATTAAT               AT^TAAT
PstI          869  CTGCAG               CTGCA^G
PvuII         836  CAGCTG               CAG^CTG
ScaI         1706  AGTACT               AGT^ACT
SexAI         216  ACCTGGT              A^CC[AT]GGT
SmlI          651  CTCAAG               C^T[CT][GA]AG
SpeI          580  ACTAGT               A^CTAGT
Van91I       1720  CCACGATATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII         291  AGATCC               [GA]^GATC[CT]
XmnI         1356  GAAGCTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AccII          63  CGCG                 CG^CG
              335  CGCG                
BciVI         823  GGATAC               GTATCC(6/5)
             1384  GGATAC              
BmyI          329  GAGCAC               G[AGT]GC[ACT]^C
              393  GGGCAC              
BpmI          308  CTGGAG               CTGGAG(16/14)
              560  CTGGAG              
BsaAI         367  TACGTG               [CT]AC^GT[GA]
             1888  CACGTG              
BseRI        1014  CTCCTC               GAGGAG(10/8)
              563  GAGGAG              
BsmAI        1030  GAGAC                GTCTC(1/5)
              221  GTCTC               
Bsp1286I      329  GAGCAC               G[AGT]GC[ACT]^C
              393  GGGCAC              
BspMI         339  ACCTGC               ACCTGC(4/8)
             1585  GCAGGT              
BstUI          63  CGCG                 CG^CG
              335  CGCG                
Cac8I         441  GCTTGC               GC[ACGT]^[ACGT]GC
              804  GCCTGC              
Cfr13I        238  GGACC                G^G[ACGT]CC
              802  GGGCC               
MaeII         368  ACGT                 A^CGT
             1889  ACGT                
MslI          906  CACTGCTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1440  CATGAGGATG          
MspA1I        836  CAGCTG               C[AC]G^C[GT]G
             1380  CAGCGG              
MvnI           63  CGCG                 CG^CG
              335  CGCG                
SapI          433  GCTCTTC              GCTCTTC(1/4)
             1359  GCTCTTC             
Sau96I        238  GGACC                G^G[ACGT]CC
              802  GGGCC               
Tsp45I         36  GTCAC                ^GT[GC]AC
             1201  GTCAC               
XcmI          614  CCATCTTGTTCTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1269  CCAGAACAGAGCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   57 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        BbsI        
BbvI        BpuAI       BsaJI       BsiYI       BslI        
BsmFI       BspHI       BsrI        BssKI       BstF5I      
BstNI       BstXI       DdeI        DpnII       DsaI        
EarI        EcoRII      EcoT14I     Fnu4HI      FokI        
HaeIII      HinfI       HphI        ItaI        Ksp632I     
MaeIII      MboI        MboII       MnlI        MseI        
MvaI        MwoI        NdeII       NlaIII      NlaIV       
PleI        RcaI        RsaI        Sau3AI      ScrFI       
SfaNI       SfcI        SspI        StuI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp509I     
TspRI       TthHB8I     
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