Restriction map for
pF1KB5062
(Circular) (Six-base) MAPSORT of: pF1KB5062.seq Check: 9 from: 1 to: 5078
pF1KB5062 5078 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AccI GT'mk_AC
Cuts at: 2116 2116
Size: 5078
Acc65I G'GTAC_C
Cuts at: 2094 2094
Size: 5078
AclI AA'CG_TT
Cuts at: 4918 4918
Size: 5078
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 211 224 271 1795 1803 2903 3335 3688 4350
Size: 13 47 1524 8 1100 432 353 662
Cuts at: 4350 211
Size: 939
Fragments arranged by size:
1524 1100 939 662 432 353 47 13
8
AflIII A'CryG_T
Cuts at: 1927 2430 4230 4511 1927
Size: 503 1800 281 2494
Fragments arranged by size:
2494 1800 503 281
AleI CACnn'nnGTG
Cuts at: 1018 1018
Size: 5078
AlwNI CAG_nnn'CTG
Cuts at: 2383 3821 2383
Size: 1438 3640
ApaLI G'TGCA_C
Cuts at: 3916 3916
Size: 5078
ApoI r'AATT_y
Cuts at: 1861 2082 4431 4687 1861
Size: 221 2349 256 2252
Fragments arranged by size:
2349 2252 256 221
AseI AT'TA_AT
Cuts at: 20 1001 20
Size: 981 4097
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5078
AvaI C'yCGr_G
Cuts at: 2098 2098
Size: 5078
BamHI G'GATC_C
Cuts at: 2103 2103
Size: 5078
BanI G'GyrC_C
Cuts at: 1336 2094 2508 2756 2791 1336
Size: 758 414 248 35 3623
Fragments arranged by size:
3623 758 414 248 35
BanII G_rGCy'C
Cuts at: 2092 3122 2092
Size: 1030 4048
BbeI G_GCGC'C
Cuts at: 2512 2760 2512
Size: 248 4830
BbsI GAAGACnn'nnnn_
Cuts at: 104 900 1046 1188 104
Size: 796 146 142 3994
Fragments arranged by size:
3994 796 146 142
BciVI GTATCCnnnnn_n'
Cuts at: 925 1486 2976 4032 5078 925
Size: 561 1490 1056 1046 925
Fragments arranged by size:
1490 1056 1046 925 561
BclI T'GATC_A
Cuts at: 2598 2598
Size: 5078
BglII A'GATC_T
Cuts at: 2593 4630 2593
Size: 2037 3041
BlpI GC'TnA_GC
Cuts at: 2190 2190
Size: 5078
Bme1580I G_kGCm'C
Cuts at: 505 2703 2796 3920 505
Size: 2198 93 1124 1663
Fragments arranged by size:
2198 1663 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 133 1330 2290 2371 2706 4850 133
Size: 1197 960 81 335 2144 361
Fragments arranged by size:
2144 1197 960 361 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3445 3445
Size: 5078
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 437 689 437
Size: 252 4826
Bpu10I CC'TnA_GC
Cuts at: 917 917
Size: 5078
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 744 2254 3133 3847 4145 744
Size: 1510 879 714 298 1677
Fragments arranged by size:
1677 1510 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 334 4782 334
Size: 4448 630
BsaAI yAC'GTr
Cuts at: 477 1998 2431 477
Size: 1521 433 3124
Fragments arranged by size:
3124 1521 433
BsaBI GATnn'nnATC
Cuts at: 6 2592 4629 6
Size: 2586 2037 455
Fragments arranged by size:
2586 2037 455
BsaHI Gr'CG_yC
Cuts at: 2509 2757 3459 4955 2509
Size: 248 702 1496 2632
Fragments arranged by size:
2632 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2267 2788 3466 3877 4024 4298 2267
Size: 521 678 411 147 274 3047
Fragments arranged by size:
3047 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1204 1234 1204
Size: 30 5048
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 686 1113 686
Size: 427 4651
BseYI C'CCAG_C
Cuts at: 1272 1387 2503 3261 3926 4334 1272
Size: 115 1116 758 665 408 2016
Fragments arranged by size:
2016 1116 758 665 408 115
BsiEI CG_ry'CG
Cuts at: 104 2620 2666 3896 104
Size: 2516 46 1230 1286
Fragments arranged by size:
2516 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 441 2092 2870 3060 3920 441
Size: 1651 778 190 860 1599
Fragments arranged by size:
1651 1599 860 778 190
BsmI GAATG_Cn'
Cuts at: 1823 1823
Size: 5078
Bsp1286I G_dGCh'C
Cuts at: 441 505 2092 2703 2796 2870 3060 3122 3920
Size: 64 1587 611 93 74 190 62 798
Cuts at: 3920 441
Size: 1599
Fragments arranged by size:
1599 1587 798 611 190 93 74 64
62
BspHI T'CATG_A
Cuts at: 317 570 1727 3510 317
Size: 253 1157 1783 1885
Fragments arranged by size:
1885 1783 1157 253
BspMI ACCTGCnnnn'nnnn_
Cuts at: 456 1684 2128 2342 2644 3025 456
Size: 1228 444 214 302 381 2509
Fragments arranged by size:
2509 1228 444 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3370 3424 4908 5073 50
Size: 3320 54 1484 165 55
Fragments arranged by size:
3320 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1787 2990 1787
Size: 1203 3875
BsrFI r'CCGG_y
Cuts at: 3076 3257 3076
Size: 181 4897
BssSI C'ACGA_G
Cuts at: 3349 4057 3349
Size: 708 4370
BstAPI GCAn_nnn'nTGC
Cuts at: 2455 2455
Size: 5078
BstBI TT'CG_AA
Cuts at: 3439 3439
Size: 5078
BstXI CCAn_nnnn'nTGG
Cuts at: 1020 1674 1680 1020
Size: 654 6 4418
Fragments arranged by size:
4418 654 6
BstYI r'GATC_y
Cuts at: 1 399 2103 2570 2593 2928 3174 3480 3492
Size: 398 1704 467 23 335 246 306 12
Cuts at: 3492 3578 4630 1
Size: 86 1052 449
Fragments arranged by size:
1704 1052 467 449 398 335 306 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 888 888
Size: 5078
BtgI C'CryG_G
Cuts at: 657 906 1439 1673 657
Size: 249 533 234 4062
Fragments arranged by size:
4062 533 249 234
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3028 3209 3372 3028
Size: 181 163 4734
Fragments arranged by size:
4734 181 163
BtsI GCAGTG_nn'
Cuts at: 1013 1434 2321 1013
Size: 421 887 3770
Fragments arranged by size:
3770 887 421
ClaI AT'CG_AT
Cuts at: 1964 1964
Size: 5078
DraI TTT'AAA
Cuts at: 1047 2077 1047
Size: 1030 4048
DrdI GACnn_nn'nnGTC
Cuts at: 2784 4128 2784
Size: 1344 3734
EaeI y'GGCC_r
Cuts at: 1682 2663 2837 3228 3255 4600 1682
Size: 981 174 391 27 1345 2160
Fragments arranged by size:
2160 1345 981 391 174 27
EagI C'GGCC_G
Cuts at: 2663 2663
Size: 5078
EarI CTCTTCn'nnn_
Cuts at: 247 548 1402 1474 3101 3311 247
Size: 301 854 72 1627 210 2014
Fragments arranged by size:
2014 1627 854 301 210 72
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3286 4028 4174 4888 3286
Size: 742 146 714 3476
Fragments arranged by size:
3476 742 714 146
EcoICRI GAG'CTC
Cuts at: 2090 2090
Size: 5078
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 211 224 271 437 689 1795 1803 2903 3335
Size: 13 47 166 252 1106 8 1100 432
Cuts at: 3335 3688 4350 211
Size: 353 662 939
Fragments arranged by size:
1106 1100 939 662 432 353 252 166
47 13 8
EcoO109I rG'GnC_Cy
Cuts at: 346 2217 346
Size: 1871 3207
EcoRI G'AATT_C
Cuts at: 2082 2082
Size: 5078
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 276 1485 3097 276
Size: 1209 1612 2257
Fragments arranged by size:
2257 1612 1209
FspI TGC'GCA
Cuts at: 2859 2859
Size: 5078
HaeII r_GCGC'y
Cuts at: 2512 2760 3990 4761 2512
Size: 248 1230 771 2829
Fragments arranged by size:
2829 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 489 521 803 835 836 868 2920 2950 2952
Size: 32 282 32 1 32 2052 30 2
Cuts at: 2952 2982 489
Size: 30 2585
Fragments arranged by size:
2585 2052 282 32 32 32 30 30
2 1
HincII GTy'rAC
Cuts at: 1119 2117 1119
Size: 998 4080
KasI G'GCGC_C
Cuts at: 2508 2756 2508
Size: 248 4830
KpnI G_GTAC'C
Cuts at: 2098 2098
Size: 5078
MfeI C'AATT_G
Cuts at: 2033 2033
Size: 5078
MluI A'CGCG_T
Cuts at: 4511 4511
Size: 5078
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 621 3837 4021 621
Size: 3216 184 1678
Fragments arranged by size:
3216 1678 184
MscI TGG'CCA
Cuts at: 1684 2839 1684
Size: 1155 3923
MslI CAynn'nnrTG
Cuts at: 1018 1552 3067 3194 1018
Size: 534 1515 127 2902
Fragments arranged by size:
2902 1515 534 127
MspA1I CmG'CkG
Cuts at: 946 1490 2189 2503 2863 3647 3892 946
Size: 544 699 314 360 784 245 2132
Fragments arranged by size:
2132 784 699 544 360 314 245
NaeI GCC'GGC
Cuts at: 3259 3259
Size: 5078
NarI GG'CG_CC
Cuts at: 2509 2757 2509
Size: 248 4830
NcoI C'CATG_G
Cuts at: 1673 1673
Size: 5078
NgoMIV G'CCGG_C
Cuts at: 3257 3257
Size: 5078
NspI r_CATG'y
Cuts at: 1931 2131 2631 4234 1931
Size: 200 500 1603 2775
Fragments arranged by size:
2775 1603 500 200
PasI CC'CwG_GG
Cuts at: 1976 1976
Size: 5078
PciI A'CATG_T
Cuts at: 1927 4230 1927
Size: 2303 2775
PflMI CCAn_nnn'nTGG
Cuts at: 1834 1834
Size: 5078
PmeI GTTT'AAAC
Cuts at: 2077 2077
Size: 5078
PmlI CAC'GTG
Cuts at: 1998 1998
Size: 5078
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1376 1408 3524 3556 1376
Size: 32 2116 32 2898
Fragments arranged by size:
2898 2116 32 32
PpuMI rG'GwC_Cy
Cuts at: 346 346
Size: 5078
PstI C_TGCA'G
Cuts at: 981 2125 2810 981
Size: 1144 685 3249
Fragments arranged by size:
3249 1144 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5078
PvuII CAG'CTG
Cuts at: 946 2503 2863 946
Size: 1557 360 3161
Fragments arranged by size:
3161 1557 360
RsrII CG'GwC_CG
Cuts at: 3273 3273
Size: 5078
SacI G_AGCT'C
Cuts at: 2092 2092
Size: 5078
SalI G'TCGA_C
Cuts at: 2115 2115
Size: 5078
SanDI GG'GwC_CC
Cuts at: 346 346
Size: 5078
SapI GCTCTTCn'nnn_
Cuts at: 548 1474 3101 3311 548
Size: 926 1627 210 2315
Fragments arranged by size:
2315 1627 926 210
SbfI CC_TGCA'GG
Cuts at: 2125 2125
Size: 5078
ScaI AGT'ACT
Cuts at: 1816 4595 1816
Size: 2779 2299
SexAI A'CCwGG_T
Cuts at: 324 324
Size: 5078
SfcI C'TryA_G
Cuts at: 33 977 1292 1497 1712 2121 2806 3774 3965
Size: 944 315 205 215 409 685 968 191
Cuts at: 3965 33
Size: 1146
Fragments arranged by size:
1146 968 944 685 409 315 215 205
191
SfoI GGC'GCC
Cuts at: 2510 2758 2510
Size: 248 4830
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5078
SmaI CCC'GGG
Cuts at: 2100 2100
Size: 5078
SmlI C'TyrA_G
Cuts at: 759 2233 3148 3862 4124 759
Size: 1474 915 714 262 1713
Fragments arranged by size:
1713 1474 915 714 262
SpeI A'CTAG_T
Cuts at: 67 688 67
Size: 621 4457
SphI G_CATG'C
Cuts at: 2131 2631 2131
Size: 500 4578
SspI AAT'ATT
Cuts at: 296 1945 2068 296
Size: 1649 123 3306
Fragments arranged by size:
3306 1649 123
StuI AGG'CCT
Cuts at: 925 964 1288 925
Size: 39 324 4715
Fragments arranged by size:
4715 324 39
StyI C'CwwG_G
Cuts at: 635 926 1518 1673 2003 2212 2566 635
Size: 291 592 155 330 209 354 3147
Fragments arranged by size:
3147 592 354 330 291 209 155
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2661 2794 3253 3462 2661
Size: 133 459 209 4277
Fragments arranged by size:
4277 459 209 133
TatI w'GTAC_w
Cuts at: 1586 1814 1930 4593 1586
Size: 228 116 2663 2071
Fragments arranged by size:
2663 2071 228 116
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4595 4627 4595
Size: 32 5046
Tth111I GACn'n_nGTC
Cuts at: 2875 2875
Size: 5078
XbaI T'CTAG_A
Cuts at: 2109 2109
Size: 5078
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 729 1384 2573 729
Size: 655 1189 3234
Fragments arranged by size:
3234 1189 655
XmaI C'CCGG_G
Cuts at: 2098 2098
Size: 5078
XmnI GAAnn'nnTTC
Cuts at: 1468 1468
Size: 5078
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PflMI PmeI PmlI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmgBI BmtI BsgI BsiWI BsmBI BspEI BsrGI BssHII
BstEII BstZ17I CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI NdeI NheI NotI NruI NsiI PacI
PfoI PshAI PsiI PspOMI PspXI PsrI SacII SfiI
SgrAI SnaBI SrfI SwaI XhoI ZraI