Restriction map for pF1KB5062
 (Linear) (Six-base) MAPSORT of: KIBB5062.seq  Check: 1020  from: 1  to: 1965



KIBB5062.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:26  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     103     116     163    1687    1695    1965 
   Size:        103      13      47    1524       8     270

  Fragments arranged by size: 

                1524     270     103      47      13       8
 
AflIII A'CryG_T

Cuts at:      0    1819    1965 
   Size:       1819     146
 
AleI CACnn'nnGTG

Cuts at:      0     910    1965 
   Size:        910    1055
 
ApoI r'AATT_y

Cuts at:      0    1753    1965 
   Size:       1753     212
 
AseI AT'TA_AT

Cuts at:      0     893    1965 
   Size:        893    1072
 
BanI G'GyrC_C

Cuts at:      0    1228    1965 
   Size:       1228     737
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     792     938    1080    1965 
   Size:        792     146     142     885

  Fragments arranged by size: 

                 885     792     146     142
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     817    1378    1965 
   Size:        817     561     587

  Fragments arranged by size: 

                 817     587     561
 
Bme1580I G_kGCm'C

Cuts at:      0     397    1965 
   Size:        397    1568
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      25    1222    1965 
   Size:         25    1197     743

  Fragments arranged by size: 

                1197     743      25
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     329     581    1965 
   Size:        329     252    1384

  Fragments arranged by size: 

                1384     329     252
 
Bpu10I CC'TnA_GC

Cuts at:      0     809    1965 
   Size:        809    1156
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     636    1965 
   Size:        636    1329
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     226    1965 
   Size:        226    1739
 
BsaAI yAC'GTr

Cuts at:      0     369    1890    1965 
   Size:        369    1521      75

  Fragments arranged by size: 

                1521     369      75
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1096    1126    1965 
   Size:       1096      30     839

  Fragments arranged by size: 

                1096     839      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     578    1005    1965 
   Size:        578     427     960

  Fragments arranged by size: 

                 960     578     427
 
BseYI C'CCAG_C

Cuts at:      0    1164    1279    1965 
   Size:       1164     115     686

  Fragments arranged by size: 

                1164     686     115
 
BsiHKAI G_wGCw'C

Cuts at:      0     333    1965 
   Size:        333    1632
 
BsmI GAATG_Cn'

Cuts at:      0    1715    1965 
   Size:       1715     250
 
Bsp1286I G_dGCh'C

Cuts at:      0     333     397    1965 
   Size:        333      64    1568

  Fragments arranged by size: 

                1568     333      64
 
BspHI T'CATG_A

Cuts at:      0     209     462    1619    1965 
   Size:        209     253    1157     346

  Fragments arranged by size: 

                1157     346     253     209
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     348    1576    1965 
   Size:        348    1228     389

  Fragments arranged by size: 

                1228     389     348
 
BsrDI GCAATG_nn'

Cuts at:      0    1679    1965 
   Size:       1679     286
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     912    1566    1572    1965 
   Size:        912     654       6     393

  Fragments arranged by size: 

                 912     654     393       6
 
BstYI r'GATC_y

Cuts at:      0     291    1965 
   Size:        291    1674
 
Bsu36I CC'TnA_GG

Cuts at:      0     780    1965 
   Size:        780    1185
 
BtgI C'CryG_G

Cuts at:      0     549     798    1331    1565    1965 
   Size:        549     249     533     234     400

  Fragments arranged by size: 

                 549     533     400     249     234
 
BtsI GCAGTG_nn'

Cuts at:      0     905    1326    1965 
   Size:        905     421     639

  Fragments arranged by size: 

                 905     639     421
 
ClaI AT'CG_AT

Cuts at:      0    1856    1965 
   Size:       1856     109
 
DraI TTT'AAA

Cuts at:      0     939    1965 
   Size:        939    1026
 
EaeI y'GGCC_r

Cuts at:      0    1574    1965 
   Size:       1574     391
 
EarI CTCTTCn'nnn_

Cuts at:      0     139     440    1294    1366    1965 
   Size:        139     301     854      72     599

  Fragments arranged by size: 

                 854     599     301     139      72
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     103     116     163     329     581    1687    1695    1965 
   Size:        103      13      47     166     252    1106       8     270

  Fragments arranged by size: 

                1106     270     252     166     103      47      13       8
            
 
EcoO109I rG'GnC_Cy

Cuts at:      0     238    1965 
   Size:        238    1727
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     168    1377    1965 
   Size:        168    1209     588

  Fragments arranged by size: 

                1209     588     168
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     381     413     695     727     728     760    1965 
   Size:        381      32     282      32       1      32    1205

  Fragments arranged by size: 

                1205     381     282      32      32      32       1
 
HincII GTy'rAC

Cuts at:      0    1011    1965 
   Size:       1011     954
 
MfeI C'AATT_G

Cuts at:      0    1925    1965 
   Size:       1925      40
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     513    1965 
   Size:        513    1452
 
MscI TGG'CCA

Cuts at:      0    1576    1965 
   Size:       1576     389
 
MslI CAynn'nnrTG

Cuts at:      0     910    1444    1965 
   Size:        910     534     521

  Fragments arranged by size: 

                 910     534     521
 
MspA1I CmG'CkG

Cuts at:      0     838    1382    1965 
   Size:        838     544     583

  Fragments arranged by size: 

                 838     583     544
 
NcoI C'CATG_G

Cuts at:      0    1565    1965 
   Size:       1565     400
 
NspI r_CATG'y

Cuts at:      0    1823    1965 
   Size:       1823     142
 
PasI CC'CwG_GG

Cuts at:      0    1868    1965 
   Size:       1868      97
 
PciI A'CATG_T

Cuts at:      0    1819    1965 
   Size:       1819     146
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1726    1965 
   Size:       1726     239
 
PmlI CAC'GTG

Cuts at:      0    1890    1965 
   Size:       1890      75
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1268    1300    1965 
   Size:       1268      32     665

  Fragments arranged by size: 

                1268     665      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     238    1965 
   Size:        238    1727
 
PstI C_TGCA'G

Cuts at:      0     873    1965 
   Size:        873    1092
 
PvuII CAG'CTG

Cuts at:      0     838    1965 
   Size:        838    1127
 
SanDI GG'GwC_CC

Cuts at:      0     238    1965 
   Size:        238    1727
 
SapI GCTCTTCn'nnn_

Cuts at:      0     440    1366    1965 
   Size:        440     926     599

  Fragments arranged by size: 

                 926     599     440
 
ScaI AGT'ACT

Cuts at:      0    1708    1965 
   Size:       1708     257
 
SexAI A'CCwGG_T

Cuts at:      0     216    1965 
   Size:        216    1749
 
SfcI C'TryA_G

Cuts at:      0     869    1184    1389    1604    1965 
   Size:        869     315     205     215     361

  Fragments arranged by size: 

                 869     361     315     215     205
 
SmlI C'TyrA_G

Cuts at:      0     651    1965 
   Size:        651    1314
 
SpeI A'CTAG_T

Cuts at:      0     580    1965 
   Size:        580    1385
 
SspI AAT'ATT

Cuts at:      0     188    1837    1960    1965 
   Size:        188    1649     123       5

  Fragments arranged by size: 

                1649     188     123       5
 
StuI AGG'CCT

Cuts at:      0     817     856    1180    1965 
   Size:        817      39     324     785

  Fragments arranged by size: 

                 817     785     324      39
 
StyI C'CwwG_G

Cuts at:      0     527     818    1410    1565    1895    1965 
   Size:        527     291     592     155     330      70

  Fragments arranged by size: 

                 592     527     330     291     155      70
 
TatI w'GTAC_w

Cuts at:      0    1478    1706    1822    1965 
   Size:       1478     228     116     143

  Fragments arranged by size: 

                1478     228     143     116
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     621    1276    1965 
   Size:        621     655     689

  Fragments arranged by size: 

                 689     655     621
 
XmnI GAAnn'nnTTC

Cuts at:      0    1360    1965 
   Size:       1360     605
 

 Enzymes that do cut:

     AcuI   AflIII     AleI     ApoI     AseI     BanI     BbsI    BciVI
 Bme1580I     BmrI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaXI
    BseRI    BseYI  BsiHKAI     BsmI Bsp1286I    BspHI    BspMI    BsrDI
    BstXI    BstYI   Bsu36I     BtgI     BtsI     ClaI     DraI     EaeI
     EarI  Eco57MI EcoO109I     FalI    Hin4I   HincII     MfeI     MmeI
     MscI     MslI   MspA1I     NcoI     NspI     PasI     PciI    PflMI
     PmlI     PpiI    PpuMI     PstI    PvuII    SanDI     SapI     ScaI
    SexAI     SfcI     SmlI     SpeI     SspI     StuI     StyI     TatI
     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI    AsiSI
     AvaI    AvrII     BaeI    BamHI    BanII     BbeI    BbvCI     BcgI
     BclI    BfrBI     BglI    BglII     BlpI    BmgBI     BmtI     BplI
    BsaBI    BsaHI    BsaWI     BsgI    BsiEI    BsiWI    BsmBI    BspEI
    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
  BstZ17I    BtgZI    CspCI   DraIII     DrdI     EagI     EciI  EcoICRI
    EcoNI    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII  HindIII
     HpaI     KasI     KpnI     MluI     NaeI     NarI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PfoI     PmeI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI    SacII
     SalI     SbfI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SphI     SrfI     SwaI    TaqII     TstI  Tth111I     XbaI     XhoI
     XmaI     ZraI
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