Restriction map for pF1KB5029
##################################
# NO CUT ENZYMES (  113 enzymes) #
##################################

AatII       AccIII      AclI        AcsI        AflII       
AgeI        AhdI        Alw44I      Aor51HI     ApaLI       
ApoI        AscI        AseI        AsnI        AspEI       
AviII       BamHI       BbeI        BbvCI       BcgI        
BclI        BfrI        BglII       BlpI        Bpu1102I    
BsaBI       BsaI        BsaWI       BseAI       BsgI        
BsiEI       BsiWI       BsmBI       Bsp1407I    BspDI       
BspEI       BsrFI       BsrGI       Bst1107I    BstBI       
BstEII      BstPI       BstZ17I     CelII       Cfr10I      
ClaI        CpoI        DraI        DraIII      EagI        
Eam1105I    EclXI       Eco47III    Eco52I      EcoNI       
EcoO65I     EcoRI       EcoRV       EcoT22I     FbaI        
FseI        FspI        HaeII       HpaI        KasI        
KspI        MamI        MfeI        MluI        MroI        
MseI        MunI        NaeI        NarI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PinAI       PmeI        
PshBI       Psp1406I    PvuI        RsrII       SacI        
SacII       SalI        SapI        SbfI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        Tru9I       XbaI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   54 enzymes) #
###################################

Acc65I       1361  GGTACC               G^GTACC
AccI         1555  GTCTAC               GT^[AC][GT]AC
AccII         257  CGCG                 CG^CG
AflIII        338  ACATGT               A^C[GA][CT]GT
AocI           38  CCTCAGG              CC^T[ACGT]AGG
ApaI          590  GGGCCC               GGGCC^C
Asp718I      1361  GGTACC               G^GTACC
AspHI        1126  GAGCAC               G[AT]GC[AT]^C
AspI          694  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvrII        1395  CCTAGG               C^CTAGG
BbrPI        1366  CACGTG               CAC^GTG
BbsI          267  GAAGAC               GAAGAC(2/6)
BbvI         1475  GCTGC                GCAGC(8/12)
BciVI         249  GGATAC               GTATCC(6/5)
BcnI         1298  CCCGG                CC^[GC]GG
BlnI         1395  CCTAGG               C^CTAGG
BpuAI         267  GAAGAC               GAAGAC(2/6)
BsaAI        1366  CACGTG               [CT]AC^GT[GA]
BsiHKAI      1126  GAGCAC               G[AT]GC[AT]^C
BsmFI          50  GTCCC                GGGAC(10/14)
BspLU11I      338  ACATGT               A^CATGT
BsrBI         777  CCGCTC               CCGCTC(-3/-3)
BsrDI        1260  CATTGC               GCAATG(2/0)
BssHII        256  GCGCGC               G^CGCGC
BssSI         630  CACGAG               CACGAG(-5/-1)
BstAPI        357  GCAGATCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI         257  CGCG                 CG^CG
BstXI         807  CCAGAGAGTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I         38  CCTCAGG              CC^T[ACGT]AGG
Eco81I         38  CCTCAGG              CC^T[ACGT]AGG
Fnu4HI       1475  GCTGC                GC^[ACGT]GC
HapII        1299  CCGG                 C^CGG
HincII       1219  GTTGAC               GT[CT]^[GA]AC
HindII       1219  GTTGAC               GT[CT]^[GA]AC
HpaII        1299  CCGG                 C^CGG
ItaI         1475  GCTGC                GC^[ACGT]GC
KpnI         1361  GGTACC               GGTAC^C
MspI         1299  CCGG                 C^CGG
MvnI          257  CGCG                 CG^CG
NciI         1298  CCCGG                CC^[GC]GG
NcoI          938  CCATGG               C^CATGG
NspI          338  ACATGT               [GA]CATG^[CT]
PflFI         694  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         240  CCAGCTGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI        1366  CACGTG               CAC^GTG
PmlI         1366  CACGTG               CAC^GTG
PshAI        1222  GACTGGCGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII         241  CAGCTG               CAG^CTG
ScaI          173  AGTACT               AGT^ACT
TseI         1475  GCTGC                G^C[AT]GC
Tsp45I        679  GTCAC                ^GT[GC]AC
Tth111I       694  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        240  CCAGCTGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          420  CCAGAAACAATTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   34 enzymes) #
###################################

AcyI         1135  GGCGTC               G[GA]^CG[CT]C
             1226  GGCGTC              
Asp700I       394  GAACGCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              411  GAAAAAGTTC          
AvaI           64  CCCGAG               C^[CT]CG[GA]G
              470  CCCGAG              
BalI          755  TGGCCA               TGG^CCA
              935  TGGCCA              
BanII         590  GGGCCC               G[GA]GC[CT]^C
              889  GAGCCC              
BglI          748  GCCATCCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              937  GCCATGGAGGC         
BpmI         1145  CTGGAG               CTGGAG(16/14)
             1501  CTGGAG              
BsaHI        1135  GGCGTC               G[GA]^CG[CT]C
             1226  GGCGTC              
BsmI         1455  GAATGC               GAATGC(1/-1)
              398  GCATTC              
BsoBI          64  CCCGAG               C^[CT]CG[GA]G
              470  CCCGAG              
BspHI          11  TCATGA               T^CATGA
             1139  TCATGA              
BstYI         359  AGATCC               [GA]^GATC[CT]
             1327  GGATCT              
DrdI          691  GACGACCCTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1108  GACCTTATTGTC        
DsaI          938  CCATGG               C^C[GA][CT]GG
             1265  CCACGG              
EaeI          755  TGGCCA               [CT]^GGCC[GA]
              935  TGGCCA              
EcoT14I       938  CCATGG               C^C[AT][AT]GG
             1395  CCTAGG              
FauI          776  CCCGC                CCCGC(4/6)
              926  CCCGC               
Hin1I        1135  GGCGTC               G[GA]^CG[CT]C
             1226  GGCGTC              
HindIII      1090  AAGCTT               A^AGCTT
             1342  AAGCTT              
MaeII        1367  ACGT                 A^CGT
             1388  ACGT                
MaeIII        679  GTCAC                ^GT[ACGT]AC
             1076  GTTAC               
MflI          359  AGATCC               [GA]^GATC[CT]
             1327  GGATCT              
MluNI         755  TGGCCA               TGG^CCA
              935  TGGCCA              
MscI          755  TGGCCA               TGG^CCA
              935  TGGCCA              
MspA1I        241  CAGCTG               C[AC]G^C[GT]G
              280  CAGCGG              
PpuMI          48  GGGTCCC              [GA]G^G[AT]CC[CT]
             1529  AGGACCC             
PstI          355  CTGCAG               CTGCA^G
              406  CTGCAG              
RcaI           11  TCATGA               T^CATGA
             1139  TCATGA              
StyI          938  CCATGG               C^C[AT][AT]GG
             1395  CCTAGG              
TaqI          288  TCGA                 T^CGA
              402  TCGA                
TfiI          285  GAATC                G^A[AT]TC
             1198  GATTC               
TthHB8I       288  TCGA                 T^CGA
              402  TCGA                
XhoII         359  AGATCC               [GA]^GATC[CT]
             1327  GGATCT              
XmnI          394  GAACGCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              411  GAAAAAGTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   57 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AlwNI       
AvaII       BanI        BfaI        BmyI        BsaJI       
BseRI       BsiYI       BslI        BsmAI       Bsp1286I    
BspMI       BsrI        BssKI       BstF5I      BstNI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       EarI        EcoO109I    EcoRII      FokI        
HaeIII      HgaI        HhaI        HinP1I      HinfI       
HphI        Ksp632I     MaeI        MboI        MboII       
MnlI        MslI        MvaI        MwoI        NdeII       
NlaIII      NlaIV       PleI        RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
Tsp509I     TspRI       
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