Restriction map for pF1KB5029
 (Linear) (Six-base) MAPSORT of: KIBB5029.seq  Check: 5276  from: 1  to: 1644



KIBB5029.orf

 With 182 enzymes: SgfI * 

                        September 18, 2009 17:00  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1073    1644 
   Size:       1073     571
 
AccI GT'mk_AC

Cuts at:      0    1556    1644 
   Size:       1556      88
 
Acc65I G'GTAC_C

Cuts at:      0    1361    1644 
   Size:       1361     283
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     464    1294    1563    1644 
   Size:        464     830     269      81

  Fragments arranged by size: 

                 830     464     269      81
 
AflIII A'CryG_T

Cuts at:      0     338    1644 
   Size:        338    1306
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     421    1644 
   Size:        421    1223
 
AlwNI CAG_nnn'CTG

Cuts at:      0     363    1208    1236    1644 
   Size:        363     845      28     408

  Fragments arranged by size: 

                 845     408     363      28
 
ApaI G_GGCC'C

Cuts at:      0     594    1644 
   Size:        594    1050
 
AvaI C'yCGr_G

Cuts at:      0      64     470    1644 
   Size:         64     406    1174

  Fragments arranged by size: 

                1174     406      64
 
AvrII C'CTAG_G

Cuts at:      0    1395    1644 
   Size:       1395     249
 
BanI G'GyrC_C

Cuts at:      0    1361    1399    1573    1644 
   Size:       1361      38     174      71

  Fragments arranged by size: 

                1361     174      71      38
 
BanII G_rGCy'C

Cuts at:      0     594     893    1644 
   Size:        594     299     751

  Fragments arranged by size: 

                 751     594     299
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     274    1644 
   Size:        274    1370
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     243    1644 
   Size:        243    1401
 
BglI GCCn_nnn'nGGC

Cuts at:      0     754     943    1644 
   Size:        754     189     701

  Fragments arranged by size: 

                 754     701     189
 
Bme1580I G_kGCm'C

Cuts at:      0     594    1644 
   Size:        594    1050
 
BmgBI CAC'GTC

Cuts at:      0    1389    1644 
   Size:       1389     255
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1166    1522    1644 
   Size:       1166     356     122

  Fragments arranged by size: 

                1166     356     122
 
Bpu10I CC'TnA_GC

Cuts at:      0    1444    1644 
   Size:       1444     200
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     881    1002    1324    1510    1644 
   Size:        881     121     322     186     134

  Fragments arranged by size: 

                 881     322     186     134     121
 
BsaAI yAC'GTr

Cuts at:      0    1368    1644 
   Size:       1368     276
 
BsaHI Gr'CG_yC

Cuts at:      0    1136    1227    1644 
   Size:       1136      91     417

  Fragments arranged by size: 

                1136     417      91
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     952     982    1644 
   Size:        952      30     662

  Fragments arranged by size: 

                 952     662      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     168     235     648    1368    1519    1612    1621    1624 
   Size:        168      67     413     720     151      93       9       3

Cuts at:   1624    1644 
   Size:         20

  Fragments arranged by size: 

                 720     413     168     151      93      67      20       9
                   3
 
BseYI C'CCAG_C

Cuts at:      0      44     971    1403    1644 
   Size:         44     927     432     241

  Fragments arranged by size: 

                 927     432     241      44
 
BsiHKAI G_wGCw'C

Cuts at:      0    1130    1644 
   Size:       1130     514
 
BsmI GAATG_Cn'

Cuts at:      0     398    1461    1644 
   Size:        398    1063     183

  Fragments arranged by size: 

                1063     398     183
 
Bsp1286I G_dGCh'C

Cuts at:      0     594     893    1130    1644 
   Size:        594     299     237     514

  Fragments arranged by size: 

                 594     514     299     237
 
BspHI T'CATG_A

Cuts at:      0      11    1139    1644 
   Size:         11    1128     505

  Fragments arranged by size: 

                1128     505      11
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     362    1073    1315    1533    1644 
   Size:        362     711     242     218     111

  Fragments arranged by size: 

                 711     362     242     218     111
 
BsrBI CCG'CTC

Cuts at:      0     779    1644 
   Size:        779     865
 
BsrDI GCAATG_nn'

Cuts at:      0    1259    1644 
   Size:       1259     385
 
BssHII G'CGCG_C

Cuts at:      0     256    1644 
   Size:        256    1388
 
BssSI C'ACGA_G

Cuts at:      0     630    1644 
   Size:        630    1014
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     363    1644 
   Size:        363    1281
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     814    1644 
   Size:        814     830
 
BstYI r'GATC_y

Cuts at:      0     359    1327    1644 
   Size:        359     968     317

  Fragments arranged by size: 

                 968     359     317
 
Bsu36I CC'TnA_GG

Cuts at:      0      39    1644 
   Size:         39    1605
 
BtgI C'CryG_G

Cuts at:      0     938    1265    1644 
   Size:        938     327     379

  Fragments arranged by size: 

                 938     379     327
 
BtsI GCAGTG_nn'

Cuts at:      0     646     982    1644 
   Size:        646     336     662

  Fragments arranged by size: 

                 662     646     336
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     697    1114    1644 
   Size:        697     417     530

  Fragments arranged by size: 

                 697     530     417
 
EaeI y'GGCC_r

Cuts at:      0     755     935    1644 
   Size:        755     180     709

  Fragments arranged by size: 

                 755     709     180
 
EarI CTCTTCn'nnn_

Cuts at:      0     483     832    1598    1613    1625    1644 
   Size:        483     349     766      15      12      19

  Fragments arranged by size: 

                 766     483     349      19      15      12
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     464    1166    1294    1522    1563    1644 
   Size:        464     702     128     228      41      81

  Fragments arranged by size: 

                 702     464     228     128      81      41
 
EcoO109I rG'GnC_Cy

Cuts at:      0      49     590     591    1530    1644 
   Size:         49     541       1     939     114

  Fragments arranged by size: 

                 939     541     114      49       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     684    1644 
   Size:        684     960
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      22      54     442     474    1241    1273    1644 
   Size:         22      32     388      32     767      32     371

  Fragments arranged by size: 

                 767     388     371      32      32      32      22
 
HincII GTy'rAC

Cuts at:      0    1221    1644 
   Size:       1221     423
 
HindIII A'AGCT_T

Cuts at:      0    1090    1342    1644 
   Size:       1090     252     302

  Fragments arranged by size: 

                1090     302     252
 
KpnI G_GTAC'C

Cuts at:      0    1365    1644 
   Size:       1365     279
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     313    1644 
   Size:        313    1331
 
MscI TGG'CCA

Cuts at:      0     757     937    1644 
   Size:        757     180     707

  Fragments arranged by size: 

                 757     707     180
 
MslI CAynn'nnrTG

Cuts at:      0     763    1211    1492    1644 
   Size:        763     448     281     152

  Fragments arranged by size: 

                 763     448     281     152
 
MspA1I CmG'CkG

Cuts at:      0     243     282    1644 
   Size:        243      39    1362

  Fragments arranged by size: 

                1362     243      39
 
NcoI C'CATG_G

Cuts at:      0     938    1644 
   Size:        938     706
 
NspI r_CATG'y

Cuts at:      0     342    1644 
   Size:        342    1302
 
PciI A'CATG_T

Cuts at:      0     338    1644 
   Size:        338    1306
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     246    1644 
   Size:        246    1398
 
PfoI T'CCnGG_A

Cuts at:      0      26    1644 
   Size:         26    1618
 
PmlI CAC'GTG

Cuts at:      0    1368    1644 
   Size:       1368     276
 
PpuMI rG'GwC_Cy

Cuts at:      0      49    1530    1644 
   Size:         49    1481     114

  Fragments arranged by size: 

                1481     114      49
 
PshAI GACnn'nnGTC

Cuts at:      0    1226    1644 
   Size:       1226     418
 
PspOMI G'GGCC_C

Cuts at:      0     590    1644 
   Size:        590    1054
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    1270    1302    1644 
   Size:       1270      32     342

  Fragments arranged by size: 

                1270     342      32
 
PstI C_TGCA'G

Cuts at:      0     359     410    1644 
   Size:        359      51    1234

  Fragments arranged by size: 

                1234     359      51
 
PvuII CAG'CTG

Cuts at:      0     243    1644 
   Size:        243    1401
 
SanDI GG'GwC_CC

Cuts at:      0      49    1644 
   Size:         49    1595
 
ScaI AGT'ACT

Cuts at:      0     175    1644 
   Size:        175    1469
 
SfcI C'TryA_G

Cuts at:      0     355     406    1557    1644 
   Size:        355      51    1151      87

  Fragments arranged by size: 

                1151     355      87      51
 
SmlI C'TyrA_G

Cuts at:      0     896     981    1339    1525    1644 
   Size:        896      85     358     186     119

  Fragments arranged by size: 

                 896     358     186     119      85
 
StyI C'CwwG_G

Cuts at:      0     938    1395    1644 
   Size:        938     457     249

  Fragments arranged by size: 

                 938     457     249
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1417    1644 
   Size:       1417     227
 
TatI w'GTAC_w

Cuts at:      0     173    1644 
   Size:        173    1471
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0       8      40     565     597     952     984    1296    1328 
   Size:          8      32     525      32     355      32     312      32

Cuts at:   1328    1644 
   Size:        316

  Fragments arranged by size: 

                 525     355     316     312      32      32      32      32
                   8
 
Tth111I GACn'n_nGTC

Cuts at:      0     697    1644 
   Size:        697     947
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     427    1644 
   Size:        427    1217
 
XmnI GAAnn'nnTTC

Cuts at:      0     398     415    1644 
   Size:        398      17    1229

  Fragments arranged by size: 

                1229     398      17
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AcuI   AflIII     AlfI    AlwNI     ApaI
     AvaI    AvrII     BanI    BanII     BbsI    BciVI     BglI Bme1580I
    BmgBI     BpmI   Bpu10I    BpuEI    BsaAI    BsaHI    BsaXI    BseRI
    BseYI  BsiHKAI     BsmI Bsp1286I    BspHI    BspMI    BsrBI    BsrDI
   BssHII    BssSI   BstAPI    BstXI    BstYI   Bsu36I     BtgI     BtsI
     DrdI     EaeI     EarI  Eco57MI EcoO109I     FalI    Hin4I   HincII
  HindIII     KpnI     MmeI     MscI     MslI   MspA1I     NcoI     NspI
     PciI    PflMI     PfoI     PmlI    PpuMI    PshAI   PspOMI     PsrI
     PstI    PvuII    SanDI     ScaI     SfcI     SmlI     StyI    TaqII
     TatI     TstI  Tth111I     XcmI     XmnI

 Enzymes that do not cut: 

    AatII     AclI     AfeI    AflII     AgeI     AhdI     AleI     AloI
    ApaLI     ApoI     AscI     AseI    AsiSI     BaeI    BamHI     BbeI
    BbvCI     BcgI     BclI    BfrBI    BglII     BlpI     BmrI     BmtI
     BplI     BsaI    BsaBI    BsaWI     BsgI    BsiEI    BsiWI    BsmBI
    BspEI    BsrFI    BsrGI    BstBI   BstEII  BstZ17I    BtgZI     ClaI
    CspCI     DraI   DraIII     EagI     EciI  EcoICRI    EcoNI    EcoRI
    EcoRV     FseI     FspI    FspAI    HaeII     HpaI     KasI     MfeI
     MluI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI     PmeI     PpiI     PsiI    PspXI     PvuI
    RsrII     SacI    SacII     SalI     SapI     SbfI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI     XbaI     XhoI     XmaI     ZraI
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