Restriction map for pF1KB5026
 (Linear) (Six-base) MAPSORT of: KIBB5026.seq  Check: 2987  from: 1  to: 1344



KIBB5026.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:26  ..

AccI GT'mk_AC

Cuts at:      0     539    1344 
   Size:        539     805
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     105     179     379     598    1250    1344 
   Size:        105      74     200     219     652      94

  Fragments arranged by size: 

                 652     219     200     105      94      74
 
AflIII A'CryG_T

Cuts at:      0     771    1344 
   Size:        771     573
 
AlwNI CAG_nnn'CTG

Cuts at:      0      48     153    1113    1228    1344 
   Size:         48     105     960     115     116

  Fragments arranged by size: 

                 960     116     115     105      48
 
ApaI G_GGCC'C

Cuts at:      0    1340    1344 
   Size:       1340       4
 
ApaLI G'TGCA_C

Cuts at:      0      97    1344 
   Size:         97    1247
 
ApoI r'AATT_y

Cuts at:      0     641     724     927    1001    1344 
   Size:        641      83     203      74     343

  Fragments arranged by size: 

                 641     343     203      83      74
 
AvaI C'yCGr_G

Cuts at:      0     371     558    1344 
   Size:        371     187     786

  Fragments arranged by size: 

                 786     371     187
 
BanI G'GyrC_C

Cuts at:      0     969    1344 
   Size:        969     375
 
BanII G_rGCy'C

Cuts at:      0    1340    1344 
   Size:       1340       4
 
BbeI G_GCGC'C

Cuts at:      0     973    1344 
   Size:        973     371
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     434    1344 
   Size:        434     910
 
Bme1580I G_kGCm'C

Cuts at:      0     101    1340    1344 
   Size:        101    1239       4

  Fragments arranged by size: 

                1239     101       4
 
BmgBI CAC'GTC

Cuts at:      0     849    1344 
   Size:        849     495
 
BmtI G_CTAG'C

Cuts at:      0      13    1344 
   Size:         13    1331
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1038    1090    1333    1344 
   Size:       1038      52     243      11

  Fragments arranged by size: 

                1038     243      52      11
 
Bpu10I CC'TnA_GC

Cuts at:      0    1023    1344 
   Size:       1023     321
 
BsaBI GATnn'nnATC

Cuts at:      0     200    1344 
   Size:        200    1144
 
BsaHI Gr'CG_yC

Cuts at:      0     970    1344 
   Size:        970     374
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     521     551    1344 
   Size:        521      30     793

  Fragments arranged by size: 

                 793     521      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1330    1344 
   Size:       1330      14
 
BseYI C'CCAG_C

Cuts at:      0     399    1344 
   Size:        399     945
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1279    1344 
   Size:       1279      65
 
BsiHKAI G_wGCw'C

Cuts at:      0     101    1344 
   Size:        101    1243
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     839    1344 
   Size:        839     505
 
Bsp1286I G_dGCh'C

Cuts at:      0     101    1340    1344 
   Size:        101    1239       4

  Fragments arranged by size: 

                1239     101       4
 
BsrDI GCAATG_nn'

Cuts at:      0     245    1344 
   Size:        245    1099
 
BsrGI T'GTAC_A

Cuts at:      0     718    1176    1344 
   Size:        718     458     168

  Fragments arranged by size: 

                 718     458     168
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     267    1344 
   Size:        267    1077
 
BstYI r'GATC_y

Cuts at:      0      65     739    1090    1344 
   Size:         65     674     351     254

  Fragments arranged by size: 

                 674     351     254      65
 
BtgI C'CryG_G

Cuts at:      0      32    1283    1344 
   Size:         32    1251      61

  Fragments arranged by size: 

                1251      61      32
 
BtsI GCAGTG_nn'

Cuts at:      0    1123    1344 
   Size:       1123     221
 
DraI TTT'AAA

Cuts at:      0     830    1344 
   Size:        830     514
 
DraIII CAC_nnn'GTG

Cuts at:      0    1193    1344 
   Size:       1193     151
 
EaeI y'GGCC_r

Cuts at:      0     474    1344 
   Size:        474     870
 
EarI CTCTTCn'nnn_

Cuts at:      0     124    1344 
   Size:        124    1220
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1338    1344 
   Size:       1338       6
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     105     179     379     598    1038    1090    1250    1333 
   Size:        105      74     200     219     440      52     160      83

Cuts at:   1333    1344 
   Size:         11

  Fragments arranged by size: 

                 440     219     200     160     105      83      74      52
                  11
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1336    1337    1344 
   Size:       1336       1       7

  Fragments arranged by size: 

                1336       7       1
 
EcoRI G'AATT_C

Cuts at:      0     724     927    1001    1344 
   Size:        724     203      74     343

  Fragments arranged by size: 

                 724     343     203      74
 
EcoRV GAT'ATC

Cuts at:      0     502    1344 
   Size:        502     842
 
HaeII r_GCGC'y

Cuts at:      0     973    1312    1344 
   Size:        973     339      32

  Fragments arranged by size: 

                 973     339      32
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     521     553     724     756    1344 
   Size:        521      32     171      32     588

  Fragments arranged by size: 

                 588     521     171      32      32
 
KasI G'GCGC_C

Cuts at:      0     969    1344 
   Size:        969     375
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     930    1134    1344 
   Size:        930     204     210

  Fragments arranged by size: 

                 930     210     204
 
MscI TGG'CCA

Cuts at:      0     476    1344 
   Size:        476     868
 
MslI CAynn'nnrTG

Cuts at:      0     265    1010    1297    1344 
   Size:        265     745     287      47

  Fragments arranged by size: 

                 745     287     265      47
 
MspA1I CmG'CkG

Cuts at:      0     921    1344 
   Size:        921     423
 
NarI GG'CG_CC

Cuts at:      0     970    1344 
   Size:        970     374
 
NcoI C'CATG_G

Cuts at:      0      32    1344 
   Size:         32    1312
 
NheI G'CTAG_C

Cuts at:      0       9    1344 
   Size:          9    1335
 
NspI r_CATG'y

Cuts at:      0     775    1344 
   Size:        775     569
 
PciI A'CATG_T

Cuts at:      0     771    1344 
   Size:        771     573
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      48      76    1344 
   Size:         48      28    1268

  Fragments arranged by size: 

                1268      48      28
 
PsiI TTA'TAA

Cuts at:      0     650    1344 
   Size:        650     694
 
PspOMI G'GGCC_C

Cuts at:      0    1336    1344 
   Size:       1336       8
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     332     364     479     511    1344 
   Size:        332      32     115      32     833

  Fragments arranged by size: 

                 833     332     115      32      32
 
PstI C_TGCA'G

Cuts at:      0     308    1118    1344 
   Size:        308     810     226

  Fragments arranged by size: 

                 810     308     226
 
PvuII CAG'CTG

Cuts at:      0     921    1344 
   Size:        921     423
 
SexAI A'CCwGG_T

Cuts at:      0      41    1344 
   Size:         41    1303
 
SfcI C'TryA_G

Cuts at:      0     170     304     932    1114    1344 
   Size:        170     134     628     182     230

  Fragments arranged by size: 

                 628     230     182     170     134
 
SfoI GGC'GCC

Cuts at:      0     971    1344 
   Size:        971     373
 
SspI AAT'ATT

Cuts at:      0     572    1344 
   Size:        572     772
 
StuI AGG'CCT

Cuts at:      0     809    1344 
   Size:        809     535
 
StyI C'CwwG_G

Cuts at:      0      15      32     777    1344 
   Size:         15      17     745     567

  Fragments arranged by size: 

                 745     567      17      15
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1287    1344 
   Size:       1287      57
 
TatI w'GTAC_w

Cuts at:      0     718    1176    1344 
   Size:        718     458     168

  Fragments arranged by size: 

                 718     458     168
 
XmnI GAAnn'nnTTC

Cuts at:      0    1005    1344 
   Size:       1005     339
 

 Enzymes that do cut:

     AccI     AcuI   AflIII    AlwNI     ApaI    ApaLI     ApoI     AvaI
     BanI    BanII     BbeI     BbsI Bme1580I    BmgBI     BmtI     BpmI
   Bpu10I    BsaBI    BsaHI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI
    BsmBI Bsp1286I    BsrDI    BsrGI    BstXI    BstYI     BtgI     BtsI
     DraI   DraIII     EaeI     EarI     EciI  Eco57MI EcoO109I    EcoRI
    EcoRV    HaeII    Hin4I     KasI     MmeI     MscI     MslI   MspA1I
     NarI     NcoI     NheI     NspI     PciI    PflMI     PsiI   PspOMI
     PsrI     PstI    PvuII    SexAI     SfcI     SfoI     SspI     StuI
     StyI    TaqII     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI     AscI     AseI    AsiSI    AvrII     BaeI
    BamHI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI     BmrI     BplI    BpuEI     BsaI    BsaAI    BsaWI    BsiEI
    BsiWI     BsmI    BspEI    BspHI    BspMI    BsrBI    BsrFI   BssHII
    BssSI   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I    BtgZI     ClaI
    CspCI     DrdI     EagI  EcoICRI    EcoNI     FalI     FseI     FspI
    FspAI   HincII  HindIII     HpaI     KpnI     MfeI     MluI     NaeI
     NdeI   NgoMIV     NotI     NruI     NsiI     PacI     PasI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI    PspXI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI     SfiI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SwaI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI
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