Restriction map for pF1KB5017
##################################
# NO CUT ENZYMES (  149 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AgeI        AhdI        Alw44I      
AlwI        Aor51HI     ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AviII       AvrII       BalI        BamHI       
BbeI        BbrPI       BbsI        BcgI        BciVI       
BclI        BfrI        BglII       BlnI        BlpI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaWI       
BseAI       BsiEI       BsiWI       BsmBI       BsmI        
Bsp1407I    BspDI       BspEI       BspHI       BspMI       
BsrDI       BsrFI       BsrGI       BssHII      Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstXI       
BstYI       BstZ17I     CelII       Cfr10I      ClaI        
CpoI        DpnII       DraI        DraIII      DrdI        
DsaI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoNI       EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        FseI        FspI        HindIII     
HpaI        KasI        KpnI        KspI        MamI        
MboI        MfeI        MflI        MluI        MluNI       
MroI        MscI        MseI        MunI        NaeI        
NarI        NcoI        NdeI        NdeII       NgoMIV      
NheI        NotI        NsiI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PstI        
PvuI        PvuII       RcaI        RsrII       SacII       
SalI        Sau3AI      SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        Tru9I       Tsp509I     Tth111I     
XbaI        XhoI        XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AccII         242  CGCG                 CG^CG
AcyI          974  GACGCC               G[GA]^CG[CT]C
AflIII        860  ACATGT               A^C[GA][CT]GT
AlwNI         766  CAGGAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          883  CCTCAGG              CC^T[ACGT]AGG
BbvCI         201  CCTCAGC              CCTCAGC(-5/-2)
BfaI          791  CTAG                 C^TAG
BglI          935  GCCACAGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI         974  GACGCC               G[GA]^CG[CT]C
BsaI          260  GAGACC               GGTCTC(1/5)
BseRI         199  CTCCTC               GAGGAG(10/8)
BsgI           89  GTGCAG               GTGCAG(16/14)
BspLU11I      860  ACATGT               A^CATGT
BstUI         242  CGCG                 CG^CG
Bsu36I        883  CCTCAGG              CC^T[ACGT]AGG
Eco81I        883  CCTCAGG              CC^T[ACGT]AGG
EcoT14I       959  CCTTGG               C^C[AT][AT]GG
HgaI          974  GACGC                GACGC(5/10)
Hin1I         974  GACGCC               G[GA]^CG[CT]C
HincII        328  GTCAAC               GT[CT]^[GA]AC
HindII        328  GTCAAC               GT[CT]^[GA]AC
MaeI          791  CTAG                 C^TAG
MaeII         670  ACGT                 A^CGT
MslI            9  CACCCTCGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI          242  CGCG                 CG^CG
NruI          241  TCGCGA               TCG^CGA
NspI          860  ACATGT               [GA]CATG^[CT]
PflMI         765  CCAGGAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI         566  GGGACCT              [GA]G^G[AT]CC[CT]
SapI          538  GAAGAGC              GCTCTTC(1/4)
SfcI           75  CTATAG               C^T[GA][CT]AG
StyI          959  CCTTGG               C^C[AT][AT]GG
TaqI           32  TCGA                 T^CGA
TfiI          889  GATTC                G^A[AT]TC
Tsp45I        163  GTCAC                ^GT[GC]AC
TthHB8I        32  TCGA                 T^CGA
Van91I        765  CCAGGAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AfaI          390  GTAC                 GT^AC
              456  GTAC                
ApaI          508  GGGCCC               GGGCC^C
              560  GGGCCC              
AvaII         567  GGACC                G^G[AT]CC
              900  GGACC               
BsmAI         245  GAGAC                GTCTC(1/5)
              260  GAGAC               
BssSI         424  CACGAG               CACGAG(-5/-1)
               13  CTCGTG              
BstF5I        420  CATCC                GGATG(2/0)
              525  CATCC               
CfoI          543  GCGC                 GCG^C
              698  GCGC                
EaeI          321  CGGCCA               [CT]^GGCC[GA]
              554  TGGCCG              
EarI          832  CTCTTC               CTCTTC(1/4)
              538  GAAGAG              
FokI          420  CATCC                GGATG(9/13)
              525  CATCC               
HaeII         542  AGCGCC               [GA]GCGC^[CT]
              697  AGCGCC              
HhaI          543  GCGC                 GCG^C
              698  GCGC                
HinP1I        543  GCGC                 G^CGC
              698  GCGC                
Ksp632I       832  CTCTTC               CTCTTC(1/4)
              538  GAAGAG              
MaeIII         59  GTAAC                ^GT[ACGT]AC
              163  GTCAC               
RsaI          390  GTAC                 GT^AC
              456  GTAC                
SacI          237  GAGCTC               GAGCT^C
              427  GAGCTC              
SfaNI          66  GATGC                GCATC(5/9)
              419  GCATC               
XcmI          647  CCACCGGGCTGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              761  CCAGCCAGGAGCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   53 enzymes) #
#########################################

AciI        AluI        AspHI       AvaI        BanI        
BanII       BbvI        BcnI        BmyI        BpmI        
BsaJI       BsiHKAI     BsiYI       BslI        BsmFI       
BsoBI       Bsp1286I    BsrBI       BsrI        BssKI       
BstNI       Cac8I       Cfr13I      DdeI        DraII       
EcoO109I    EcoRII      FauI        Fnu4HI      HaeIII      
HapII       HinfI       HpaII       HphI        ItaI        
MboII       MnlI        MspA1I      MspI        MvaI        
MwoI        NciI        NlaIII      NlaIV       PleI        
Sau96I      ScrFI       SmaI        SmlI        TseI        
TspRI       XmaCI       XmaI        
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