Restriction map for pF1KB5017
 (Linear) (Six-base) MAPSORT of: KIBB5017.seq  Check: 915  from: 1  to: 1014



KIBB5017.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:26  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     557     972    1014 
   Size:        557     415      42

  Fragments arranged by size: 

                 557     415      42
 
AflIII A'CryG_T

Cuts at:      0     860    1014 
   Size:        860     154
 
AleI CACnn'nnGTG

Cuts at:      0      13    1014 
   Size:         13    1001
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     114     817    1014 
   Size:        114     703     197

  Fragments arranged by size: 

                 703     197     114
 
AlwNI CAG_nnn'CTG

Cuts at:      0     771    1014 
   Size:        771     243
 
ApaI G_GGCC'C

Cuts at:      0     512     564    1014 
   Size:        512      52     450

  Fragments arranged by size: 

                 512     450      52
 
AvaI C'yCGr_G

Cuts at:      0     313     563     805    1014 
   Size:        313     250     242     209

  Fragments arranged by size: 

                 313     250     242     209
 
BanI G'GyrC_C

Cuts at:      0     124     379     736    1014 
   Size:        124     255     357     278

  Fragments arranged by size: 

                 357     278     255     124
 
BanII G_rGCy'C

Cuts at:      0     241     431     512     564     718    1014 
   Size:        241     190      81      52     154     296

  Fragments arranged by size: 

                 296     241     190     154      81      52
 
BbvCI CC'TCA_GC

Cuts at:      0     202    1014 
   Size:        202     812
 
BglI GCCn_nnn'nGGC

Cuts at:      0     941    1014 
   Size:        941      73
 
Bme1580I G_kGCm'C

Cuts at:      0     127     512     552     564    1014 
   Size:        127     385      40      12     450

  Fragments arranged by size: 

                 450     385     127      40      12
 
BmgBI CAC'GTC

Cuts at:      0     671    1014 
   Size:        671     343
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      80     617    1014 
   Size:         80     537     397

  Fragments arranged by size: 

                 537     397      80
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     318     577     744     909    1014 
   Size:        318     259     167     165     105

  Fragments arranged by size: 

                 318     259     167     165     105
 
Bpu10I CC'TnA_GC

Cuts at:      0     202     916    1014 
   Size:        202     714      98

  Fragments arranged by size: 

                 714     202      98
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      57     196     954    1014 
   Size:         57     139     758      60

  Fragments arranged by size: 

                 758     139      60      57
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     254    1014 
   Size:        254     760
 
BsaHI Gr'CG_yC

Cuts at:      0     975    1014 
   Size:        975      39
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     190    1014 
   Size:        190     824
 
BseYI C'CCAG_C

Cuts at:      0     172     613     877    1014 
   Size:        172     441     264     137

  Fragments arranged by size: 

                 441     264     172     137
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     110    1014 
   Size:        110     904
 
BsiHKAI G_wGCw'C

Cuts at:      0      84     241     431    1014 
   Size:         84     157     190     583

  Fragments arranged by size: 

                 583     190     157      84
 
Bsp1286I G_dGCh'C

Cuts at:      0      84     127     241     431     512     552     564     718 
   Size:         84      43     114     190      81      40      12     154

Cuts at:    718    1014 
   Size:        296

  Fragments arranged by size: 

                 296     190     154     114      84      81      43      40
                  12
 
BsrBI CCG'CTC

Cuts at:      0     232     266     288    1014 
   Size:        232      34      22     726

  Fragments arranged by size: 

                 726     232      34      22
 
BssSI C'ACGA_G

Cuts at:      0      13     424    1014 
   Size:         13     411     590

  Fragments arranged by size: 

                 590     411      13
 
Bsu36I CC'TnA_GG

Cuts at:      0     884    1014 
   Size:        884     130
 
BtsI GCAGTG_nn'

Cuts at:      0     125    1014 
   Size:        125     889
 
EaeI y'GGCC_r

Cuts at:      0     321     554    1014 
   Size:        321     233     460

  Fragments arranged by size: 

                 460     321     233
 
EarI CTCTTCn'nnn_

Cuts at:      0     533     838    1014 
   Size:        533     305     176

  Fragments arranged by size: 

                 533     305     176
 
EcoICRI GAG'CTC

Cuts at:      0     239     429    1014 
   Size:        239     190     585

  Fragments arranged by size: 

                 585     239     190
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     318     557     577     744     909     972    1014 
   Size:        318     239      20     167     165      63      42

  Fragments arranged by size: 

                 318     239     167     165      63      42      20
 
EcoO109I rG'GnC_Cy

Cuts at:      0     509     560     567    1014 
   Size:        509      51       7     447

  Fragments arranged by size: 

                 509     447      51       7
 
HaeII r_GCGC'y

Cuts at:      0     546     701    1014 
   Size:        546     155     313

  Fragments arranged by size: 

                 546     313     155
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     880     912     986    1014 
   Size:        880      32      74      28

  Fragments arranged by size: 

                 880      74      32      28
 
HincII GTy'rAC

Cuts at:      0     330    1014 
   Size:        330     684
 
MslI CAynn'nnrTG

Cuts at:      0      13    1014 
   Size:         13    1001
 
MspA1I CmG'CkG

Cuts at:      0     515     577     631     643    1014 
   Size:        515      62      54      12     371

  Fragments arranged by size: 

                 515     371      62      54      12
 
NruI TCG'CGA

Cuts at:      0     243    1014 
   Size:        243     771
 
NspI r_CATG'y

Cuts at:      0     864    1014 
   Size:        864     150
 
PciI A'CATG_T

Cuts at:      0     860    1014 
   Size:        860     154
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     771    1014 
   Size:        771     243
 
PpuMI rG'GwC_Cy

Cuts at:      0     567    1014 
   Size:        567     447
 
PspOMI G'GGCC_C

Cuts at:      0     508     560    1014 
   Size:        508      52     454

  Fragments arranged by size: 

                 508     454      52
 
SacI G_AGCT'C

Cuts at:      0     241     431    1014 
   Size:        241     190     583

  Fragments arranged by size: 

                 583     241     190
 
SapI GCTCTTCn'nnn_

Cuts at:      0     533    1014 
   Size:        533     481
 
SfcI C'TryA_G

Cuts at:      0      75    1014 
   Size:         75     939
 
SmaI CCC'GGG

Cuts at:      0     315     565     807    1014 
   Size:        315     250     242     207

  Fragments arranged by size: 

                 315     250     242     207
 
SmlI C'TyrA_G

Cuts at:      0      36     211     969    1014 
   Size:         36     175     758      45

  Fragments arranged by size: 

                 758     175      45      36
 
StyI C'CwwG_G

Cuts at:      0     959    1014 
   Size:        959      55
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     316    1014 
   Size:        316     698
 
TatI w'GTAC_w

Cuts at:      0     455    1014 
   Size:        455     559
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     404     436    1014 
   Size:        404      32     578

  Fragments arranged by size: 

                 578     404      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     654     768    1014 
   Size:        654     114     246

  Fragments arranged by size: 

                 654     246     114
 
XmaI C'CCGG_G

Cuts at:      0     313     563     805    1014 
   Size:        313     250     242     209

  Fragments arranged by size: 

                 313     250     242     209
 

 Enzymes that do cut:

     AcuI   AflIII     AleI     AlfI    AlwNI     ApaI     AvaI     BanI
    BanII    BbvCI     BglI Bme1580I    BmgBI     BmrI     BpmI   Bpu10I
    BpuEI     BsaI    BsaHI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I
    BsrBI    BssSI   Bsu36I     BtsI     EaeI     EarI  EcoICRI  Eco57MI
 EcoO109I    HaeII    Hin4I   HincII     MslI   MspA1I     NruI     NspI
     PciI    PflMI    PpuMI   PspOMI     SacI     SapI     SfcI     SmaI
     SmlI     StyI    TaqII     TatI     TstI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AloI    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BbeI     BbsI     BcgI    BciVI     BclI    BfrBI
    BglII     BlpI     BmtI     BplI    BsaAI    BsaBI    BsaWI    BsaXI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrDI
    BsrFI    BsrGI   BssHII   BstAPI    BstBI   BstEII    BstXI    BstYI
  BstZ17I     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EciI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NsiI     PacI     PasI     PfoI     PmeI     PmlI     PpiI    PshAI
     PsiI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII    SacII
     SalI    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
  Tth111I     XbaI     XhoI     XmnI     ZraI
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