Restriction map for pF1KB5012
##################################
# NO CUT ENZYMES (  128 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      ApaI        ApaLI       AscI        AseI        
AsnI        Asp718I     AspEI       AspI        AvaI        
AviII       AvrII       BalI        BanI        BanII       
BbeI        BbvCI       BclI        BfrI        BlnI        
BlpI        Bpu1102I    BsaBI       BsaHI       BsaWI       
BseAI       BsiEI       BsiWI       BsmBI       BsmI        
BsoBI       BspEI       BspLU11I    BsrDI       BssHII      
BstAPI      BstBI       BstEII      BstPI       BstUI       
CelII       CpoI        DraI        DraIII      DrdI        
EagI        Eam1105I    EclXI       Eco52I      EcoNI       
EcoO65I     EcoRI       EcoRV       EcoT22I     FauI        
FbaI        FseI        FspI        Hin1I       HindIII     
HpaI        HphI        KasI        KpnI        KspI        
MamI        MfeI        MluI        MluNI       MroI        
MscI        MunI        MvnI        NaeI        NarI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmeI        PshAI       PshBI       Psp1406I    
PstI        PvuI        RsrII       SacI        SacII       
SapI        SbfI        ScaI        SexAI       SfcI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspI        
StuI        SwaI        Tth111I     Van91I      XcmI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AcsI          367  AAATTC               [GA]^AATT[CT]
AocI         1477  CCTGAGG              CC^T[ACGT]AGG
Aor51HI      1406  AGCGCT               AGC^GCT
ApoI          367  AAATTC               [GA]^AATT[CT]
BamHI          99  GGATCC               G^GATCC
BbrPI         112  CACGTG               CAC^GTG
BbsI          880  GAAGAC               GAAGAC(2/6)
BciVI        1259  GTATCC               GTATCC(6/5)
BglI          349  GCCAGGTTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         648  AGATCT               A^GATCT
BpuAI         880  GAAGAC               GAAGAC(2/6)
BsaAI         112  CACGTG               [CT]AC^GT[GA]
BsaI          622  GAGACC               GGTCTC(1/5)
BsgI         1483  GTGCAG               GTGCAG(16/14)
BsmFI        1121  GGGAC                GGGAC(10/14)
Bsp1407I      119  TGTACA               T^GTACA
BspDI          58  ATCGAT               AT^CGAT
BspMI           6  GCAGGT               ACCTGC(4/8)
BsrBI        1048  GAGCGG               CCGCTC(-3/-3)
BsrFI        1430  ACCGGC               [GA]^CCGG[CT]
BsrGI         119  TGTACA               T^GTACA
BsrI          209  ACTGG                ACTGG(1/-1)
BssSI         301  CACGAG               CACGAG(-5/-1)
Bst1107I      802  GTATAC               GTA^TAC
BstZ17I       802  GTATAC               GTA^TAC
Bsu36I       1477  CCTGAGG              CC^T[ACGT]AGG
Cfr10I       1430  ACCGGC               [GA]^CCGG[CT]
ClaI           58  ATCGAT               AT^CGAT
EaeI         1469  TGGCCG               [CT]^GGCC[GA]
EarI          580  GAAGAG               CTCTTC(1/4)
Eco47III     1406  AGCGCT               AGC^GCT
Eco81I       1477  CCTGAGG              CC^T[ACGT]AGG
HgaI          539  GCGTC                GACGC(5/10)
HincII        193  GTCGAC               GT[CT]^[GA]AC
HindII        193  GTCGAC               GT[CT]^[GA]AC
Ksp632I       580  GAAGAG               CTCTTC(1/4)
MaeII         113  ACGT                 A^CGT
NdeI          905  CATATG               CA^TATG
NspI          386  ACATGC               [GA]CATG^[CT]
PleI         1372  GACTC                GAGTC(4/5)
PmaCI         112  CACGTG               CAC^GTG
PmlI          112  CACGTG               CAC^GTG
PpuMI        1538  AGGACCC              [GA]G^G[AT]CC[CT]
SalI          193  GTCGAC               G^TCGAC
SspBI         119  TGTACA               T^GTACA
TfiI         1569  GAATC                G^A[AT]TC
Tsp45I       1499  GTGAC                ^GT[GC]AC
XbaI          243  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AccI          193  GTCGAC               GT^[AC][GT]AC
              802  GTATAC              
AlwNI         670  CAGGCACTG            CAG[ACGT][ACGT][ACGT]^CTG
             1594  CAGAAGCTG           
Asp700I       375  GAACCCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              646  GAAGATCTTC          
AspHI         985  GAGCAC               G[AT]GC[AT]^C
             1003  GTGCTC              
AvaII         762  GGACC                G^G[AT]CC
             1539  GGACC               
BcgI         1198  CGAGCTGCCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
              185  GCAAGACTGTCG        
BfaI          244  CTAG                 C^TAG
             1363  CTAG                
BsiHKAI       985  GAGCAC               G[AT]GC[AT]^C
             1003  GTGCTC              
DraII          38  AGGCCCT              [GA]G^G[ACGT]CC[CT]
             1538  AGGACCC             
DsaI         1340  CCATGG               C^C[GA][CT]GG
             1466  CCATGG              
EcoO109I       38  AGGCCCT              [GA]G^G[ACGT]CC[CT]
             1538  AGGACCC             
HaeII        1359  GGCGCT               [GA]GCGC^[CT]
             1406  AGCGCT              
HinfI        1372  GACTC                G^A[ACGT]TC
             1569  GAATC               
MaeI          244  CTAG                 C^TAG
             1363  CTAG                
MaeIII         53  GTAAC                ^GT[ACGT]AC
             1499  GTGAC               
NcoI         1340  CCATGG               C^CATGG
             1466  CCATGG              
SmlI         1006  CTCAAG               C^T[CT][GA]AG
             1240  CTCAAG              
XmnI          375  GAACCCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              646  GAAGATCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   64 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        BbvI        
BcnI        BmyI        BpmI        BsaJI       BseRI       
BsiYI       BslI        BsmAI       Bsp1286I    BspHI       
BssKI       BstF5I      BstNI       BstXI       BstYI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
EcoRII      EcoT14I     Fnu4HI      FokI        HaeIII      
HapII       HhaI        HinP1I      HpaII       ItaI        
MboI        MboII       MflI        MnlI        MseI        
MslI        MspA1I      MspI        MvaI        MwoI        
NciI        NdeII       NlaIII      NlaIV       PvuII       
RcaI        RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       StyI        TaqI        Tru9I       TseI        
Tsp509I     TspRI       TthHB8I     XhoII       
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