Restriction map for pF1KB5012
 (Linear) (Six-base) MAPSORT of: KIBB5012.seq  Check: 6033  from: 1  to: 1629



KIBB5012.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:26  ..

AccI GT'mk_AC

Cuts at:      0     194     803    1629 
   Size:        194     609     826

  Fragments arranged by size: 

                 826     609     194
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      40    1057    1629 
   Size:         40    1017     572

  Fragments arranged by size: 

                1017     572      40
 
AfeI AGC'GCT

Cuts at:      0    1408    1629 
   Size:       1408     221
 
AlwNI CAG_nnn'CTG

Cuts at:      0     675    1599    1629 
   Size:        675     924      30

  Fragments arranged by size: 

                 924     675      30
 
ApoI r'AATT_y

Cuts at:      0     367    1629 
   Size:        367    1262
 
BamHI G'GATC_C

Cuts at:      0      99    1629 
   Size:         99    1530
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     887    1629 
   Size:        887     742
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     174     208    1187    1221    1629 
   Size:        174      34     979      34     408

  Fragments arranged by size: 

                 979     408     174      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1270    1629 
   Size:       1270     359
 
BglI GCCn_nnn'nGGC

Cuts at:      0     355    1629 
   Size:        355    1274
 
BglII A'GATC_T

Cuts at:      0     648    1629 
   Size:        648     981
 
Bme1580I G_kGCm'C

Cuts at:      0     502    1629 
   Size:        502    1127
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     219    1629 
   Size:        219    1410
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1243    1288    1359    1629 
   Size:       1243      45      71     270

  Fragments arranged by size: 

                1243     270      71      45
 
Bpu10I CC'TnA_GC

Cuts at:      0      43    1629 
   Size:         43    1586
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     991    1225    1629 
   Size:        991     234     404

  Fragments arranged by size: 

                 991     404     234
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     616    1629 
   Size:        616    1013
 
BsaAI yAC'GTr

Cuts at:      0     114    1629 
   Size:        114    1515
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     337     367    1357    1387    1629 
   Size:        337      30     990      30     242

  Fragments arranged by size: 

                 990     337     242      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     301     589    1099    1629 
   Size:        301     288     510     530

  Fragments arranged by size: 

                 530     510     301     288
 
BseYI C'CCAG_C

Cuts at:      0     690    1503    1629 
   Size:        690     813     126

  Fragments arranged by size: 

                 813     690     126
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1504    1629 
   Size:       1504     125
 
BsiHKAI G_wGCw'C

Cuts at:      0     989    1007    1629 
   Size:        989      18     622

  Fragments arranged by size: 

                 989     622      18
 
Bsp1286I G_dGCh'C

Cuts at:      0     502     989    1007    1629 
   Size:        502     487      18     622

  Fragments arranged by size: 

                 622     502     487      18
 
BspHI T'CATG_A

Cuts at:      0     371     524    1493    1629 
   Size:        371     153     969     136

  Fragments arranged by size: 

                 969     371     153     136
 
BsrBI CCG'CTC

Cuts at:      0    1050    1629 
   Size:       1050     579
 
BsrFI r'CCGG_y

Cuts at:      0    1430    1629 
   Size:       1430     199
 
BsrGI T'GTAC_A

Cuts at:      0     119    1629 
   Size:        119    1510
 
BssSI C'ACGA_G

Cuts at:      0     301    1629 
   Size:        301    1328
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     759     861    1221    1341    1629 
   Size:        759     102     360     120     288

  Fragments arranged by size: 

                 759     360     288     120     102
 
BstYI r'GATC_y

Cuts at:      0      99     513     648    1589    1629 
   Size:         99     414     135     941      40

  Fragments arranged by size: 

                 941     414     135      99      40
 
BstZ17I GTA'TAC

Cuts at:      0     804    1629 
   Size:        804     825
 
Bsu36I CC'TnA_GG

Cuts at:      0    1478    1629 
   Size:       1478     151
 
BtgI C'CryG_G

Cuts at:      0    1340    1466    1629 
   Size:       1340     126     163

  Fragments arranged by size: 

                1340     163     126
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1333    1629 
   Size:       1333     296
 
ClaI AT'CG_AT

Cuts at:      0      59    1629 
   Size:         59    1570
 
EaeI y'GGCC_r

Cuts at:      0    1469    1629 
   Size:       1469     160
 
EarI CTCTTCn'nnn_

Cuts at:      0     575    1629 
   Size:        575    1054
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      40    1057    1243    1288    1359    1629 
   Size:         40    1017     186      45      71     270

  Fragments arranged by size: 

                1017     270     186      71      45      40
 
EcoO109I rG'GnC_Cy

Cuts at:      0      39    1539    1629 
   Size:         39    1500      90

  Fragments arranged by size: 

                1500      90      39
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     667     735    1629 
   Size:        667      68     894

  Fragments arranged by size: 

                 894     667      68
 
HaeII r_GCGC'y

Cuts at:      0    1363    1410    1629 
   Size:       1363      47     219

  Fragments arranged by size: 

                1363     219      47
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1357    1389    1629 
   Size:       1357      32     240

  Fragments arranged by size: 

                1357     240      32
 
HincII GTy'rAC

Cuts at:      0     195    1629 
   Size:        195    1434
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     389    1629 
   Size:        389    1240
 
MslI CAynn'nnrTG

Cuts at:      0     772     859    1345    1629 
   Size:        772      87     486     284

  Fragments arranged by size: 

                 772     486     284      87
 
MspA1I CmG'CkG

Cuts at:      0     799    1233    1266    1306    1379    1629 
   Size:        799     434      33      40      73     250

  Fragments arranged by size: 

                 799     434     250      73      40      33
 
NcoI C'CATG_G

Cuts at:      0    1340    1466    1629 
   Size:       1340     126     163

  Fragments arranged by size: 

                1340     163     126
 
NdeI CA'TA_TG

Cuts at:      0     906    1629 
   Size:        906     723
 
NspI r_CATG'y

Cuts at:      0     390    1629 
   Size:        390    1239
 
PfoI T'CCnGG_A

Cuts at:      0    1220    1520    1629 
   Size:       1220     300     109

  Fragments arranged by size: 

                1220     300     109
 
PmlI CAC'GTG

Cuts at:      0     114    1629 
   Size:        114    1515
 
PpuMI rG'GwC_Cy

Cuts at:      0    1539    1629 
   Size:       1539      90
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     688     720    1629 
   Size:        688      32     909

  Fragments arranged by size: 

                 909     688      32
 
PvuII CAG'CTG

Cuts at:      0    1233    1266    1379    1629 
   Size:       1233      33     113     250

  Fragments arranged by size: 

                1233     250     113      33
 
SalI G'TCGA_C

Cuts at:      0     193    1629 
   Size:        193    1436
 
SmlI C'TyrA_G

Cuts at:      0    1006    1240    1629 
   Size:       1006     234     389

  Fragments arranged by size: 

                1006     389     234
 
StyI C'CwwG_G

Cuts at:      0     546     752    1340    1466    1629 
   Size:        546     206     588     126     163

  Fragments arranged by size: 

                 588     546     206     163     126
 
TatI w'GTAC_w

Cuts at:      0     119     944    1424    1629 
   Size:        119     825     480     205

  Fragments arranged by size: 

                 825     480     205     119
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1574    1606    1629 
   Size:       1574      32      23

  Fragments arranged by size: 

                1574      32      23
 
XbaI T'CTAG_A

Cuts at:      0     243    1629 
   Size:        243    1386
 
XmnI GAAnn'nnTTC

Cuts at:      0     379     650    1629 
   Size:        379     271     979

  Fragments arranged by size: 

                 979     379     271
 

 Enzymes that do cut:

     AccI     AcuI     AfeI    AlwNI     ApoI    BamHI     BbsI     BcgI
    BciVI     BglI    BglII Bme1580I     BmrI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I
    BspHI    BsrBI    BsrFI    BsrGI    BssSI    BstXI    BstYI  BstZ17I
   Bsu36I     BtgI    BtgZI     ClaI     EaeI     EarI  Eco57MI EcoO109I
     FalI    HaeII    Hin4I   HincII     MmeI     MslI   MspA1I     NcoI
     NdeI     NspI     PfoI     PmlI    PpuMI     PsrI    PvuII     SalI
     SmlI     StyI     TatI     TstI     XbaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI     BanI    BanII     BbeI    BbvCI     BclI
    BfrBI     BlpI    BmgBI     BmtI     BplI    BsaBI    BsaHI    BsaWI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspMI    BsrDI   BssHII
   BstAPI    BstBI   BstEII     BtsI    CspCI     DraI   DraIII     DrdI
     EagI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FseI     FspI
    FspAI  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI
     NaeI     NarI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI     PciI    PflMI     PmeI     PpiI    PshAI     PsiI   PspOMI
    PspXI     PstI     PvuI    RsrII     SacI    SacII    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
    TaqII  Tth111I     XcmI     XhoI     XmaI     ZraI
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