Restriction map for pF1KB5010
 (Linear) (Six-base) MAPSORT of: KIBB5010.seq  Check: 555  from: 1  to: 1857



KIBB5010.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:26  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1306    1413    1726    1857 
   Size:       1306     107     313     131

  Fragments arranged by size: 

                1306     313     131     107
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     170     524    1857 
   Size:        170     354    1333

  Fragments arranged by size: 

                1333     354     170
 
AfeI AGC'GCT

Cuts at:      0      49    1606    1857 
   Size:         49    1557     251

  Fragments arranged by size: 

                1557     251      49
 
AleI CACnn'nnGTG

Cuts at:      0     476     980    1857 
   Size:        476     504     877

  Fragments arranged by size: 

                 877     504     476
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     401     433    1857 
   Size:        401      32    1424

  Fragments arranged by size: 

                1424     401      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1281    1857 
   Size:       1281     576
 
ApaLI G'TGCA_C

Cuts at:      0     385     985    1069    1153    1857 
   Size:        385     600      84      84     704

  Fragments arranged by size: 

                 704     600     385      84      84
 
AvaI C'yCGr_G

Cuts at:      0      51     289     343     822     876    1158    1857 
   Size:         51     238      54     479      54     282     699

  Fragments arranged by size: 

                 699     479     282     238      54      54      51
 
AvrII C'CTAG_G

Cuts at:      0     241    1857 
   Size:        241    1616
 
BanI G'GyrC_C

Cuts at:      0     285    1857 
   Size:        285    1572
 
BanII G_rGCy'C

Cuts at:      0     623    1622    1817    1857 
   Size:        623     999     195      40

  Fragments arranged by size: 

                 999     623     195      40
 
BglI GCCn_nnn'nGGC

Cuts at:      0     488    1336    1857 
   Size:        488     848     521

  Fragments arranged by size: 

                 848     521     488
 
Bme1580I G_kGCm'C

Cuts at:      0     343     389     847     989    1073    1157    1268    1790 
   Size:        343      46     458     142      84      84     111     522

Cuts at:   1790    1857 
   Size:         67

  Fragments arranged by size: 

                 522     458     343     142     111      84      84      67
                  46
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1536    1635    1857 
   Size:       1536      99     222

  Fragments arranged by size: 

                1536     222      99
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1590    1857 
   Size:       1590     267
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1349    1831    1857 
   Size:       1349     482      26

  Fragments arranged by size: 

                1349     482      26
 
Bpu10I CC'TnA_GC

Cuts at:      0     191    1857 
   Size:        191    1666
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     448     607    1036    1764    1857 
   Size:        448     159     429     728      93

  Fragments arranged by size: 

                 728     448     429     159      93
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1561    1857 
   Size:       1561     296
 
BsaAI yAC'GTr

Cuts at:      0     719    1857 
   Size:        719    1138
 
BsaHI Gr'CG_yC

Cuts at:      0    1039    1857 
   Size:       1039     818
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     205     235    1857 
   Size:        205      30    1622

  Fragments arranged by size: 

                1622     205      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1114    1534    1857 
   Size:       1114     420     323

  Fragments arranged by size: 

                1114     420     323
 
BseYI C'CCAG_C

Cuts at:      0      30    1353    1669    1857 
   Size:         30    1323     316     188

  Fragments arranged by size: 

                1323     316     188      30
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1256    1620    1857 
   Size:       1256     364     237

  Fragments arranged by size: 

                1256     364     237
 
BsiEI CG_ry'CG

Cuts at:      0     354     858     999    1003    1857 
   Size:        354     504     141       4     854

  Fragments arranged by size: 

                 854     504     354     141       4
 
BsiHKAI G_wGCw'C

Cuts at:      0     389     623     989    1073    1157    1622    1857 
   Size:        389     234     366      84      84     465     235

  Fragments arranged by size: 

                 465     389     366     235     234      84      84
 
BsmI GAATG_Cn'

Cuts at:      0    1459    1841    1857 
   Size:       1459     382      16

  Fragments arranged by size: 

                1459     382      16
 
Bsp1286I G_dGCh'C

Cuts at:      0     343     389     623     847     989    1073    1157    1268 
   Size:        343      46     234     224     142      84      84     111

Cuts at:   1268    1622    1790    1817    1857 
   Size:        354     168      27      40

  Fragments arranged by size: 

                 354     343     234     224     168     142     111      84
                  84      46      40      27
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1306    1413    1726    1857 
   Size:       1306     107     313     131

  Fragments arranged by size: 

                1306     313     131     107
 
BsrBI CCG'CTC

Cuts at:      0     495     645     704    1233    1857 
   Size:        495     150      59     529     624

  Fragments arranged by size: 

                 624     529     495     150      59
 
BsrDI GCAATG_nn'

Cuts at:      0    1024    1857 
   Size:       1024     833
 
BsrFI r'CCGG_y

Cuts at:      0     135     557     655     957     977     998    1851    1857 
   Size:        135     422      98     302      20      21     853       6

  Fragments arranged by size: 

                 853     422     302     135      98      21      20       6
            
 
BssHII G'CGCG_C

Cuts at:      0     112     449     897    1857 
   Size:        112     337     448     960

  Fragments arranged by size: 

                 960     448     337     112
 
BssSI C'ACGA_G

Cuts at:      0     808    1228    1600    1857 
   Size:        808     420     372     257

  Fragments arranged by size: 

                 808     420     372     257
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1643    1857 
   Size:       1643     214
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     478    1465    1809    1857 
   Size:        478     987     344      48

  Fragments arranged by size: 

                 987     478     344      48
 
BstYI r'GATC_y

Cuts at:      0     467    1857 
   Size:        467    1390
 
Bsu36I CC'TnA_GG

Cuts at:      0    1219    1857 
   Size:       1219     638
 
BtgI C'CryG_G

Cuts at:      0     230     303    1256    1790    1857 
   Size:        230      73     953     534      67

  Fragments arranged by size: 

                 953     534     230      73      67
 
BtsI GCAGTG_nn'

Cuts at:      0     429     924    1254    1857 
   Size:        429     495     330     603

  Fragments arranged by size: 

                 603     495     429     330
 
DraIII CAC_nnn'GTG

Cuts at:      0    1418    1643    1785    1857 
   Size:       1418     225     142      72

  Fragments arranged by size: 

                1418     225     142      72
 
EaeI y'GGCC_r

Cuts at:      0     158     233     351     480     855     968    1000    1077 
   Size:        158      75     118     129     375     113      32      77

Cuts at:   1077    1409    1857 
   Size:        332     448

  Fragments arranged by size: 

                 448     375     332     158     129     118     113      77
                  75      32
 
EagI C'GGCC_G

Cuts at:      0     351     855    1000    1857 
   Size:        351     504     145     857

  Fragments arranged by size: 

                 857     504     351     145
 
EarI CTCTTCn'nnn_

Cuts at:      0     146     685    1838    1857 
   Size:        146     539    1153      19

  Fragments arranged by size: 

                1153     539     146      19
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     976    1857 
   Size:        976     881
 
EcoICRI GAG'CTC

Cuts at:      0     621    1620    1857 
   Size:        621     999     237

  Fragments arranged by size: 

                 999     621     237
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     170     524    1349    1831    1857 
   Size:        170     354     825     482      26

  Fragments arranged by size: 

                 825     482     354     170      26
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1367    1857 
   Size:       1367     490
 
EcoO109I rG'GnC_Cy

Cuts at:      0     245     627    1047    1359    1796    1857 
   Size:        245     382     420     312     437      61

  Fragments arranged by size: 

                 437     420     382     312     245      61
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1587    1758    1857 
   Size:       1587     171      99

  Fragments arranged by size: 

                1587     171      99
 
FseI GG_CCGG'CC

Cuts at:      0    1855    1857 
   Size:       1855       2
 
FspI TGC'GCA

Cuts at:      0     298     428    1678    1762    1857 
   Size:        298     130    1250      84      95

  Fragments arranged by size: 

                1250     298     130      95      84
 
FspAI rTGC'GCAy

Cuts at:      0     428    1857 
   Size:        428    1429
 
HaeII r_GCGC'y

Cuts at:      0      51     666    1170    1300    1608    1857 
   Size:         51     615     504     130     308     249

  Fragments arranged by size: 

                 615     504     308     249     130      51
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      10      42     205     237     870     902    1857 
   Size:         10      32     163      32     633      32     955

  Fragments arranged by size: 

                 955     633     163      32      32      32      10
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     893    1857 
   Size:        893     964
 
MscI TGG'CCA

Cuts at:      0     482     970    1079    1411    1857 
   Size:        482     488     109     332     446

  Fragments arranged by size: 

                 488     482     446     332     109
 
MslI CAynn'nnrTG

Cuts at:      0     476     980    1438    1857 
   Size:        476     504     458     419

  Fragments arranged by size: 

                 504     476     458     419
 
MspA1I CmG'CkG

Cuts at:      0     232     422     590     758    1083    1094    1258    1857 
   Size:        232     190     168     168     325      11     164     599

  Fragments arranged by size: 

                 599     325     232     190     168     168     164      11
            
 
NaeI GCC'GGC

Cuts at:      0     137     959    1853    1857 
   Size:        137     822     894       4

  Fragments arranged by size: 

                 894     822     137       4
 
NcoI C'CATG_G

Cuts at:      0    1790    1857 
   Size:       1790      67
 
NgoMIV G'CCGG_C

Cuts at:      0     135     957    1851    1857 
   Size:        135     822     894       6

  Fragments arranged by size: 

                 894     822     135       6
 
NotI GC'GGCC_GC

Cuts at:      0     351     855    1857 
   Size:        351     504    1002

  Fragments arranged by size: 

                1002     504     351
 
NspI r_CATG'y

Cuts at:      0     864    1190    1450    1857 
   Size:        864     326     260     407

  Fragments arranged by size: 

                 864     407     326     260
 
PasI CC'CwG_GG

Cuts at:      0     104    1857 
   Size:        104    1753
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1418    1857 
   Size:       1418     439
 
PfoI T'CCnGG_A

Cuts at:      0    1472    1857 
   Size:       1472     385
 
PmlI CAC'GTG

Cuts at:      0     719    1857 
   Size:        719    1138
 
PpuMI rG'GwC_Cy

Cuts at:      0     627    1047    1359    1796    1857 
   Size:        627     420     312     437      61

  Fragments arranged by size: 

                 627     437     420     312      61
 
PspXI vC'TCGA_Gb

Cuts at:      0     876    1857 
   Size:        876     981
 
PstI C_TGCA'G

Cuts at:      0      18    1857 
   Size:         18    1839
 
PvuII CAG'CTG

Cuts at:      0     590    1857 
   Size:        590    1267
 
SacI G_AGCT'C

Cuts at:      0     623    1622    1857 
   Size:        623     999     235

  Fragments arranged by size: 

                 999     623     235
 
SacII CC_GC'GG

Cuts at:      0     233    1259    1857 
   Size:        233    1026     598

  Fragments arranged by size: 

                1026     598     233
 
SanDI GG'GwC_CC

Cuts at:      0    1359    1796    1857 
   Size:       1359     437      61

  Fragments arranged by size: 

                1359     437      61
 
SapI GCTCTTCn'nnn_

Cuts at:      0     685    1857 
   Size:        685    1172
 
SfcI C'TryA_G

Cuts at:      0      14     585    1857 
   Size:         14     571    1272

  Fragments arranged by size: 

                1272     571      14
 
SgrAI Cr'CCGG_yG

Cuts at:      0     557     655     977    1857 
   Size:        557      98     322     880

  Fragments arranged by size: 

                 880     557     322      98
 
SmlI C'TyrA_G

Cuts at:      0     463     622     876    1051    1743    1857 
   Size:        463     159     254     175     692     114

  Fragments arranged by size: 

                 692     463     254     175     159     114
 
StuI AGG'CCT

Cuts at:      0     274     442    1738    1857 
   Size:        274     168    1296     119

  Fragments arranged by size: 

                1296     274     168     119
 
StyI C'CwwG_G

Cuts at:      0     241     269    1490    1733    1790    1857 
   Size:        241      28    1221     243      57      67

  Fragments arranged by size: 

                1221     243     241      67      57      28
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1654    1792    1857 
   Size:       1654     138      65

  Fragments arranged by size: 

                1654     138      65
 
XhoI C'TCGA_G

Cuts at:      0     876    1857 
   Size:        876     981
 
XmnI GAAnn'nnTTC

Cuts at:      0     694     835    1857 
   Size:        694     141    1022

  Fragments arranged by size: 

                1022     694     141
 

 Enzymes that do cut:

     AarI     AcuI     AfeI     AleI     AloI    AlwNI    ApaLI     AvaI
    AvrII     BanI    BanII     BglI Bme1580I     BmrI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspMI    BsrBI    BsrDI
    BsrFI   BssHII    BssSI   BstAPI    BstXI    BstYI   Bsu36I     BtgI
     BtsI   DraIII     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I     FalI     FseI     FspI    FspAI    HaeII    Hin4I
     MmeI     MscI     MslI   MspA1I     NaeI     NcoI   NgoMIV     NotI
     NspI     PasI    PflMI     PfoI     PmlI    PpuMI    PspXI     PstI
    PvuII     SacI    SacII    SanDI     SapI     SfcI    SgrAI     SmlI
     StuI     StyI    TaqII     XhoI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI    AflII   AflIII     AgeI     AhdI
     AlfI     ApaI     ApoI     AscI     AseI    AsiSI     BaeI    BamHI
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII
     BlpI    BmgBI     BmtI    BsaBI    BsaWI    BsiWI    BsmBI    BspEI
    BspHI    BsrGI    BstBI   BstEII  BstZ17I    BtgZI     ClaI    CspCI
     DraI     DrdI    EcoRI    EcoRV   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     NarI     NdeI     NheI     NruI     NsiI
     PacI     PciI     PmeI     PpiI    PshAI     PsiI   PspOMI     PsrI
     PvuI    RsrII     SalI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI
     TatI     TstI  Tth111I     XbaI     XcmI     XmaI     ZraI
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