Restriction map for pF1KB5006
##################################
# NO CUT ENZYMES (  123 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AlwNI       Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AviII       AvrII       BbrPI       BbsI        BcgI        
BciVI       BclI        BfrI        BlnI        BpuAI       
BsaAI       BsaBI       BsaWI       BseAI       BsgI        
BsiWI       BsmBI       BsmI        Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstZ17I     Cfr10I      ClaI        
CpoI        DraI        DraIII      DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      EcoNI       
EcoO65I     EcoRV       EcoT22I     FbaI        FseI        
FspI        HgaI        HincII      HindII      HpaI        
KpnI        KspI        MaeII       MamI        MfeI        
MluI        MroI        MunI        NaeI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflMI       
PinAI       PmaCI       PmeI        PmlI        PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacII       
SalI        SbfI        ScaI        SexAI       SfuI        
SgrAI       SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        SwaI        Tsp45I      Van91I      
XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   55 enzymes) #
###################################

AccII         469  CGCG                 CG^CG
AcyI           45  GGCGCC               G[GA]^CG[CT]C
AocI         1289  CCTCAGG              CC^T[ACGT]AGG
AspI          151  GACCGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI         1214  CCCGGG               C^[CT]CG[GA]G
BalI          974  TGGCCA               TGG^CCA
BamHI        1196  GGATCC               G^GATCC
BanI           45  GGCGCC               G^G[CT][GA]CC
BanII         559  GAGCTC               G[GA]GC[CT]^C
BbeI           45  GGCGCC               GGCGC^C
BbvCI        1255  GCTGAGG              CCTCAGC(-5/-2)
BglI         1084  GCCCACCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         420  AGATCT               A^GATCT
BsaHI          45  GGCGCC               G[GA]^CG[CT]C
BsaI         1295  GAGACC               GGTCTC(1/5)
BsiEI          23  CGACCG               CG[GA][CT]^CG
BsoBI        1214  CCCGGG               C^[CT]CG[GA]G
BspMI         316  ACCTGC               ACCTGC(4/8)
BsrDI         273  CATTGC               GCAATG(2/0)
BstF5I       1300  CATCC                GGATG(2/0)
BstUI         469  CGCG                 CG^CG
Bsu36I       1289  CCTCAGG              CC^T[ACGT]AGG
DsaI           56  CCATGG               C^C[GA][CT]GG
Eco81I       1289  CCTCAGG              CC^T[ACGT]AGG
EcoRI         783  GAATTC               G^AATTC
FokI         1300  CATCC                GGATG(9/13)
HaeII          45  GGCGCC               [GA]GCGC^[CT]
Hin1I          45  GGCGCC               G[GA]^CG[CT]C
HindIII       863  AAGCTT               A^AGCTT
KasI           45  GGCGCC               G^GCGCC
MaeIII       1025  GTAAC                ^GT[ACGT]AC
MluNI         974  TGGCCA               TGG^CCA
MscI          974  TGGCCA               TGG^CCA
MseI          410  TTAA                 T^TAA
MvnI          469  CGCG                 CG^CG
NarI           45  GGCGCC               GG^CGCC
NcoI           56  CCATGG               C^CATGG
PflFI         151  GACCGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI        1133  AGGTCCT              [GA]G^G[AT]CC[CT]
PshAI         202  GACTCCAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI         1138  CTGCAG               CTGCA^G
PvuII        1174  CAGCTG               CAG^CTG
SacI          559  GAGCTC               GAGCT^C
SapI         1332  GAAGAGC              GCTCTTC(1/4)
SfaNI         135  GATGC                GCATC(5/9)
SfiI         1083  GGCCCACCTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI           45  GGCGCC               GGC^GCC
SmaI         1214  CCCGGG               CCC^GGG
StuI          998  AGGCCT               AGG^CCT
TaqI          951  TCGA                 T^CGA
Tru9I         410  TTAA                 T^TAA
Tth111I       151  GACCGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I       951  TCGA                 T^CGA
XmaCI        1214  CCCGGG               C^CCGGG
XmaI         1214  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AcsI          226  AAATTC               [GA]^AATT[CT]
              783  GAATTC              
ApoI          226  AAATTC               [GA]^AATT[CT]
              783  GAATTC              
AspHI         559  GAGCTC               G[AT]GC[AT]^C
              807  GAGCAC              
AvaII         150  GGACC                G^G[AT]CC
             1134  GGTCC               
BfaI          911  CTAG                 C^TAG
             1036  CTAG                
BlpI          484  GCTAAGC              GC^T[ACGT]AGC
              721  GCTGAGC             
BpmI          204  CTCCAG               CTGGAG(16/14)
              610  CTGGAG              
Bpu1102I      484  GCTAAGC              GC^T[ACGT]AGC
              721  GCTGAGC             
BsiHKAI       559  GAGCTC               G[AT]GC[AT]^C
              807  GAGCAC              
BsmFI         149  GGGAC                GGGAC(10/14)
              269  GGGAC               
BsrBI          30  GAGCGG               CCGCTC(-3/-3)
              823  GAGCGG              
BstXI         165  CCACTACACTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              287  CCATGAAGGTGG        
CelII         484  GCTAAGC              GC^T[ACGT]AGC
              721  GCTGAGC             
EaeI          974  TGGCCA               [CT]^GGCC[GA]
             1091  TGGCCG              
HapII        1074  CCGG                 C^CGG
             1215  CCGG                
HpaII        1074  CCGG                 C^CGG
             1215  CCGG                
MaeI          911  CTAG                 C^TAG
             1036  CTAG                
MspI         1074  CCGG                 C^CGG
             1215  CCGG                
SmlI          661  CTCAAG               C^T[CT][GA]AG
              745  CTCAAG              
TfiI          363  GATTC                G^A[AT]TC
              446  GAATC               


#########################################
# 3 OR MORE CUT ENZYMES (   60 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        BbvI        
BcnI        BmyI        BsaJI       BseRI       BsiYI       
BslI        BsmAI       Bsp1286I    BsrI        BssKI       
BstNI       BstYI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraII       EarI        EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      HaeIII      
HhaI        HinP1I      HinfI       HphI        ItaI        
Ksp632I     MboI        MboII       MflI        MnlI        
MslI        MspA1I      MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PleI        RsaI        
Sau3AI      Sau96I      ScrFI       SfcI        StyI        
TseI        Tsp509I     TspRI       XcmI        XhoII       
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