Restriction map for pF1KB5006
 (Linear) (Six-base) MAPSORT of: KIBB5006.seq  Check: 3109  from: 1  to: 1377



KIBB5006.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:26  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     325    1377 
   Size:        325    1052
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     121    1377 
   Size:        121    1256
 
AleI CACnn'nnGTG

Cuts at:      0     814    1377 
   Size:        814     563
 
ApoI r'AATT_y

Cuts at:      0     226     783    1377 
   Size:        226     557     594

  Fragments arranged by size: 

                 594     557     226
 
AvaI C'yCGr_G

Cuts at:      0    1214    1377 
   Size:       1214     163
 
BamHI G'GATC_C

Cuts at:      0    1196    1377 
   Size:       1196     181
 
BanI G'GyrC_C

Cuts at:      0      45    1377 
   Size:         45    1332
 
BanII G_rGCy'C

Cuts at:      0     563    1377 
   Size:        563     814
 
BbeI G_GCGC'C

Cuts at:      0      49    1377 
   Size:         49    1328
 
BbvCI CC'TCA_GC

Cuts at:      0    1256    1377 
   Size:       1256     121
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1090    1377 
   Size:       1090     287
 
BglII A'GATC_T

Cuts at:      0     420    1377 
   Size:        420     957
 
BlpI GC'TnA_GC

Cuts at:      0     485     722    1377 
   Size:        485     237     655

  Fragments arranged by size: 

                 655     485     237
 
Bme1580I G_kGCm'C

Cuts at:      0     124     832    1377 
   Size:        124     708     545

  Fragments arranged by size: 

                 708     545     124
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1305    1373    1377 
   Size:       1305      68       4

  Fragments arranged by size: 

                1305      68       4
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     189     631    1377 
   Size:        189     442     746

  Fragments arranged by size: 

                 746     442     189
 
Bpu10I CC'TnA_GC

Cuts at:      0    1256    1377 
   Size:       1256     121
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     646     730    1377 
   Size:        646      84     647

  Fragments arranged by size: 

                 647     646      84
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1289    1377 
   Size:       1289      88
 
BsaHI Gr'CG_yC

Cuts at:      0      46    1377 
   Size:         46    1331
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     214     244    1377 
   Size:        214      30    1133

  Fragments arranged by size: 

                1133     214      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      25    1273    1276    1339    1377 
   Size:         25    1248       3      63      38

  Fragments arranged by size: 

                1248      63      38      25       3
 
BseYI C'CCAG_C

Cuts at:      0     667    1172    1377 
   Size:        667     505     205

  Fragments arranged by size: 

                 667     505     205
 
BsiEI CG_ry'CG

Cuts at:      0      26    1377 
   Size:         26    1351
 
BsiHKAI G_wGCw'C

Cuts at:      0     563     811    1377 
   Size:        563     248     566

  Fragments arranged by size: 

                 566     563     248
 
Bsp1286I G_dGCh'C

Cuts at:      0     124     563     811     832    1377 
   Size:        124     439     248      21     545

  Fragments arranged by size: 

                 545     439     248     124      21
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     325    1377 
   Size:        325    1052
 
BsrBI CCG'CTC

Cuts at:      0      32     825    1377 
   Size:         32     793     552

  Fragments arranged by size: 

                 793     552      32
 
BsrDI GCAATG_nn'

Cuts at:      0     272    1377 
   Size:        272    1105
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     172     294    1377 
   Size:        172     122    1083

  Fragments arranged by size: 

                1083     172     122
 
BstYI r'GATC_y

Cuts at:      0     420     594    1196    1377 
   Size:        420     174     602     181

  Fragments arranged by size: 

                 602     420     181     174
 
Bsu36I CC'TnA_GG

Cuts at:      0    1290    1377 
   Size:       1290      87
 
BtgI C'CryG_G

Cuts at:      0      56    1377 
   Size:         56    1321
 
BtsI GCAGTG_nn'

Cuts at:      0     690    1377 
   Size:        690     687
 
EaeI y'GGCC_r

Cuts at:      0     974    1091    1377 
   Size:        974     117     286

  Fragments arranged by size: 

                 974     286     117
 
EarI CTCTTCn'nnn_

Cuts at:      0     425     785    1072    1327    1377 
   Size:        425     360     287     255      50

  Fragments arranged by size: 

                 425     360     287     255      50
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     458    1060    1377 
   Size:        458     602     317

  Fragments arranged by size: 

                 602     458     317
 
EcoICRI GAG'CTC

Cuts at:      0     561    1377 
   Size:        561     816
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     121     189     631    1377 
   Size:        121      68     442     746

  Fragments arranged by size: 

                 746     442     121      68
 
EcoO109I rG'GnC_Cy

Cuts at:      0     945    1032    1062    1134    1377 
   Size:        945      87      30      72     243

  Fragments arranged by size: 

                 945     243      87      72      30
 
EcoRI G'AATT_C

Cuts at:      0     783    1377 
   Size:        783     594
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1083    1377 
   Size:       1083     294
 
HaeII r_GCGC'y

Cuts at:      0      49    1377 
   Size:         49    1328
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      56      88     146     178     214     246    1377 
   Size:         56      32      58      32      36      32    1131

  Fragments arranged by size: 

                1131      58      56      36      32      32      32
 
HindIII A'AGCT_T

Cuts at:      0     863    1377 
   Size:        863     514
 
KasI G'GCGC_C

Cuts at:      0      45    1377 
   Size:         45    1332
 
MscI TGG'CCA

Cuts at:      0     976    1377 
   Size:        976     401
 
MslI CAynn'nnrTG

Cuts at:      0     292     814    1315    1377 
   Size:        292     522     501      62

  Fragments arranged by size: 

                 522     501     292      62
 
MspA1I CmG'CkG

Cuts at:      0      51     555    1176    1194    1377 
   Size:         51     504     621      18     183

  Fragments arranged by size: 

                 621     504     183      51      18
 
NarI GG'CG_CC

Cuts at:      0      46    1377 
   Size:         46    1331
 
NcoI C'CATG_G

Cuts at:      0      56    1377 
   Size:         56    1321
 
PpuMI rG'GwC_Cy

Cuts at:      0    1134    1377 
   Size:       1134     243
 
PshAI GACnn'nnGTC

Cuts at:      0     206    1377 
   Size:        206    1171
 
PstI C_TGCA'G

Cuts at:      0    1142    1377 
   Size:       1142     235
 
PvuII CAG'CTG

Cuts at:      0    1176    1377 
   Size:       1176     201
 
SacI G_AGCT'C

Cuts at:      0     563    1377 
   Size:        563     814
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1327    1377 
   Size:       1327      50
 
SfcI C'TryA_G

Cuts at:      0     871     994    1138    1377 
   Size:        871     123     144     239

  Fragments arranged by size: 

                 871     239     144     123
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    1090    1377 
   Size:       1090     287
 
SfoI GGC'GCC

Cuts at:      0      47    1377 
   Size:         47    1330
 
SmaI CCC'GGG

Cuts at:      0    1216    1377 
   Size:       1216     161
 
SmlI C'TyrA_G

Cuts at:      0     661     745    1377 
   Size:        661      84     632

  Fragments arranged by size: 

                 661     632      84
 
StuI AGG'CCT

Cuts at:      0    1000    1377 
   Size:       1000     377
 
StyI C'CwwG_G

Cuts at:      0      56     764    1274    1377 
   Size:         56     708     510     103

  Fragments arranged by size: 

                 708     510     103      56
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      40     167    1377 
   Size:         40     127    1210

  Fragments arranged by size: 

                1210     127      40
 
TatI w'GTAC_w

Cuts at:      0     836    1377 
   Size:        836     541
 
Tth111I GACn'n_nGTC

Cuts at:      0     154    1377 
   Size:        154    1223
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      63     291     627    1173    1377 
   Size:         63     228     336     546     204

  Fragments arranged by size: 

                 546     336     228     204      63
 
XmaI C'CCGG_G

Cuts at:      0    1214    1377 
   Size:       1214     163
 

 Enzymes that do cut:

     AarI     AcuI     AleI     ApoI     AvaI    BamHI     BanI    BanII
     BbeI    BbvCI     BglI    BglII     BlpI Bme1580I     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaHI    BsaXI    BseRI    BseYI    BsiEI
  BsiHKAI Bsp1286I    BspMI    BsrBI    BsrDI    BstXI    BstYI   Bsu36I
     BtgI     BtsI     EaeI     EarI     EciI  EcoICRI  Eco57MI EcoO109I
    EcoRI     FalI    HaeII    Hin4I  HindIII     KasI     MscI     MslI
   MspA1I     NarI     NcoI    PpuMI    PshAI     PstI    PvuII     SacI
     SapI     SfcI     SfiI     SfoI     SmaI     SmlI     StuI     StyI
    TaqII     TatI  Tth111I     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI    AvrII     BaeI     BbsI     BcgI    BciVI     BclI    BfrBI
    BmgBI     BmrI     BmtI    BsaAI    BsaBI    BsaWI     BsgI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII  BstZ17I    BtgZI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI    EcoNI    EcoRV     FseI     FspI    FspAI
   HincII     HpaI     KpnI     MfeI     MluI     MmeI     NaeI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI     PsiI   PspOMI
    PspXI     PsrI     PvuI    RsrII    SacII     SalI    SanDI     SbfI
     ScaI    SexAI     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI
     SspI     SwaI     TstI     XbaI     XhoI     XmnI     ZraI
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