Restriction map for pF1KB4998
##################################
# NO CUT ENZYMES (  151 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AflII       AgeI        AhdI        Alw44I      AlwNI       
AocI        Aor51HI     ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AvaI        AviII       
BalI        BamHI       BanI        BanII       BbeI        
BbrPI       BcgI        BciVI       BclI        BfrI        
BglI        BlpI        BmyI        Bpu1102I    BsaAI       
BsaBI       BsaWI       BseAI       BseRI       BsgI        
BsiHKAI     BsiWI       BsmBI       BsmI        BsoBI       
Bsp1286I    BspDI       BspEI       BspHI       BspLU11I    
BsrDI       BsrFI       BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstXI       BstZ17I     
Bsu36I      CelII       Cfr10I      ClaI        CpoI        
DraI        DraIII      Eam1105I    Eco47III    Eco81I      
EcoNI       EcoO65I     EcoRI       EcoRV       EcoT22I     
FbaI        FseI        FspI        HaeII       HindIII     
HpaI        HphI        KasI        KpnI        MaeIII      
MamI        MfeI        MluI        MluNI       MroI        
MscI        MseI        MslI        MunI        NaeI        
NarI        NdeI        NgoMIV      NheI        NotI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PstI        
PvuII       RcaI        RsrII       SacI        SapI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspI        StuI        SwaI        
Tru9I       Tsp45I      Tsp509I     Tth111I     Van91I      
XbaI        XcmI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   33 enzymes) #
###################################

AccI          474  GTCGAC               GT^[AC][GT]AC
AflIII        125  ACGTGT               A^C[GA][CT]GT
AvrII         364  CCTAGG               C^CTAGG
BbsI           97  GTCTTC               GAAGAC(2/6)
BfaI          365  CTAG                 C^TAG
BglII         532  AGATCT               A^GATCT
BlnI          364  CCTAGG               C^CTAGG
BpmI          528  CTCCAG               CTGGAG(16/14)
BpuAI          97  GTCTTC               GAAGAC(2/6)
Bsp1407I      128  TGTACA               T^GTACA
BspMI         323  GCAGGT               ACCTGC(4/8)
BsrGI         128  TGTACA               T^GTACA
BsrI          580  CCAGT                ACTGG(1/-1)
BssHII        268  GCGCGC               G^CGCGC
DraII         550  AGGTCCC              [GA]G^G[ACGT]CC[CT]
DrdI          477  GACCTCCAAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I      550  AGGTCCC              [GA]G^G[ACGT]CC[CT]
HincII        474  GTCGAC               GT[CT]^[GA]AC
HindII        474  GTCGAC               GT[CT]^[GA]AC
MaeI          365  CTAG                 C^TAG
MaeII         125  ACGT                 A^CGT
NcoI          221  CCATGG               C^CATGG
NlaIII        222  CATG                 CATG^
NruI          610  TCGCGA               TCG^CGA
PpuMI         550  AGGTCCC              [GA]G^G[AT]CC[CT]
PvuI          613  CGATCG               CGAT^CG
SalI          474  GTCGAC               G^TCGAC
SfaNI         217  GATGC                GCATC(5/9)
SfcI          429  CTACAG               C^T[GA][CT]AG
SmlI           46  CTCAAG               C^T[CT][GA]AG
SspBI         128  TGTACA               T^GTACA
TfiI          595  GAATC                G^A[AT]TC
TspRI         581  CAGTG                CA[GC]TG[ACGT][ACGT]^


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AcyI          347  GACGCC               G[GA]^CG[CT]C
              371  GGCGTC              
AluI            4  AGCT                 AG^CT
              251  AGCT                
BbvCI          87  GCTGAGG              CCTCAGC(-5/-2)
              202  GCTGAGG             
BsaHI         347  GACGCC               G[GA]^CG[CT]C
              371  GGCGTC              
BsrBI         435  CCGCTC               CCGCTC(-3/-3)
              650  GAGCGG              
BstF5I        132  CATCC                GGATG(2/0)
              216  GGATG               
BstYI         490  AGATCC               [GA]^GATC[CT]
              532  AGATCT              
Cac8I         248  GCGAGC               GC[ACGT]^[ACGT]GC
              268  GCGCGC              
DdeI           88  CTGAG                C^T[ACGT]AG
              203  CTGAG               
EarI          618  GAAGAG               CTCTTC(1/4)
              640  GAAGAG              
FokI          132  CATCC                GGATG(9/13)
              216  GGATG               
Hin1I         347  GACGCC               G[GA]^CG[CT]C
              371  GGCGTC              
Ksp632I       618  GAAGAG               CTCTTC(1/4)
              640  GAAGAG              
KspI          136  CCGCGG               CCGC^GG
              162  CCGCGG              
MflI          490  AGATCC               [GA]^GATC[CT]
              532  AGATCT              
MspA1I        136  CCGCGG               C[AC]G^C[GT]G
              162  CCGCGG              
SacII         136  CCGCGG               CCGC^GG
              162  CCGCGG              
XhoII         490  AGATCC               [GA]^GATC[CT]
              532  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   56 enzymes) #
#########################################

AccII       AciI        AfaI        AlwI        AvaII       
BbvI        BcnI        BsaI        BsaJI       BsiEI       
BsiYI       BslI        BsmAI       BsmFI       BssKI       
BstNI       BstUI       CfoI        Cfr13I      DpnII       
DsaI        EaeI        EagI        EclXI       Eco52I      
EcoRII      EcoT14I     FauI        Fnu4HI      HaeIII      
HapII       HgaI        HhaI        HinP1I      HinfI       
HpaII       ItaI        MboI        MboII       MnlI        
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIV       PleI        RsaI        Sau3AI      
Sau96I      ScrFI       StyI        TaqI        TseI        
TthHB8I     
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