Restriction map for pF1KB4998
 (Linear) (Six-base) MAPSORT of: KIBB4998.seq  Check: 279  from: 1  to: 663



KIBB4998.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:26  ..

AccI GT'mk_AC

Cuts at:      0     475     663 
   Size:        475     188
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     659     663 
   Size:        659       4
 
AflIII A'CryG_T

Cuts at:      0     125     663 
   Size:        125     538
 
AvrII C'CTAG_G

Cuts at:      0     364     663 
   Size:        364     299
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      90     663 
   Size:         90     573
 
BbvCI CC'TCA_GC

Cuts at:      0      88     203     663 
   Size:         88     115     460

  Fragments arranged by size: 

                 460     115      88
 
BglII A'GATC_T

Cuts at:      0     532     663 
   Size:        532     131
 
BmgBI CAC'GTC

Cuts at:      0     126     663 
   Size:        126     537
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     574     663 
   Size:        574      89
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     513     663 
   Size:        513     150
 
Bpu10I CC'TnA_GC

Cuts at:      0      88     203     663 
   Size:         88     115     460

  Fragments arranged by size: 

                 460     115      88
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      31     663 
   Size:         31     632
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     416     531     563     663 
   Size:        416     115      32     100

  Fragments arranged by size: 

                 416     115     100      32
 
BsaHI Gr'CG_yC

Cuts at:      0     348     372     663 
   Size:        348      24     291

  Fragments arranged by size: 

                 348     291      24
 
BsiEI CG_ry'CG

Cuts at:      0      12     246     279     475     616     663 
   Size:         12     234      33     196     141      47

  Fragments arranged by size: 

                 234     196     141      47      33      12
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     314     663 
   Size:        314     349
 
BsrBI CCG'CTC

Cuts at:      0     437     652     663 
   Size:        437     215      11

  Fragments arranged by size: 

                 437     215      11
 
BsrGI T'GTAC_A

Cuts at:      0     128     663 
   Size:        128     535
 
BssHII G'CGCG_C

Cuts at:      0     268     663 
   Size:        268     395
 
BstYI r'GATC_y

Cuts at:      0     490     532     663 
   Size:        490      42     131

  Fragments arranged by size: 

                 490     131      42
 
BtgI C'CryG_G

Cuts at:      0     136     162     221     663 
   Size:        136      26      59     442

  Fragments arranged by size: 

                 442     136      59      26
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     483     663 
   Size:        483     180
 
EaeI y'GGCC_r

Cuts at:      0       9     243     276     663 
   Size:          9     234      33     387

  Fragments arranged by size: 

                 387     234      33       9
 
EagI C'GGCC_G

Cuts at:      0       9     243     276     663 
   Size:          9     234      33     387

  Fragments arranged by size: 

                 387     234      33       9
 
EarI CTCTTCn'nnn_

Cuts at:      0     613     635     663 
   Size:        613      22      28

  Fragments arranged by size: 

                 613      28      22
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     513     659     663 
   Size:        513     146       4

  Fragments arranged by size: 

                 513     146       4
 
EcoO109I rG'GnC_Cy

Cuts at:      0     551     663 
   Size:        551     112
 
HincII GTy'rAC

Cuts at:      0     476     663 
   Size:        476     187
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     376     663 
   Size:        376     287
 
MspA1I CmG'CkG

Cuts at:      0     138     164     663 
   Size:        138      26     499

  Fragments arranged by size: 

                 499     138      26
 
NcoI C'CATG_G

Cuts at:      0     221     663 
   Size:        221     442
 
NruI TCG'CGA

Cuts at:      0     612     663 
   Size:        612      51
 
PfoI T'CCnGG_A

Cuts at:      0     388     565     663 
   Size:        388     177      98

  Fragments arranged by size: 

                 388     177      98
 
PpuMI rG'GwC_Cy

Cuts at:      0     551     663 
   Size:        551     112
 
PvuI CG_AT'CG

Cuts at:      0     616     663 
   Size:        616      47
 
SacII CC_GC'GG

Cuts at:      0     139     165     663 
   Size:        139      26     498

  Fragments arranged by size: 

                 498     139      26
 
SalI G'TCGA_C

Cuts at:      0     474     663 
   Size:        474     189
 
SfcI C'TryA_G

Cuts at:      0     429     663 
   Size:        429     234
 
SmlI C'TyrA_G

Cuts at:      0      46     663 
   Size:         46     617
 
StyI C'CwwG_G

Cuts at:      0     152     221     364     663 
   Size:        152      69     143     299

  Fragments arranged by size: 

                 299     152     143      69
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     437     461     513     551     663 
   Size:        437      24      52      38     112

  Fragments arranged by size: 

                 437     112      52      38      24
 
TatI w'GTAC_w

Cuts at:      0     128     663 
   Size:        128     535
 

 Enzymes that do cut:

     AccI     AcuI   AflIII    AvrII     BbsI    BbvCI    BglII    BmgBI
     BmrI     BpmI   Bpu10I    BpuEI     BsaI    BsaHI    BsiEI    BspMI
    BsrBI    BsrGI   BssHII    BstYI     BtgI     DrdI     EaeI     EagI
     EarI  Eco57MI EcoO109I   HincII     MmeI   MspA1I     NcoI     NruI
     PfoI    PpuMI     PvuI    SacII     SalI     SfcI     SmlI     StyI
    TaqII     TatI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI     BaeI    BamHI     BanI    BanII     BbeI
     BcgI    BciVI     BclI    BfrBI     BglI     BlpI Bme1580I     BmtI
     BplI    BsaAI    BsaBI    BsaWI    BsaXI    BseRI    BseYI     BsgI
  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BsrDI
    BsrFI    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     EciI  EcoICRI
    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
    Hin4I  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI
     MslI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PmeI     PmlI     PpiI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI    PvuII    RsrII
     SacI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI
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