Restriction map for
pF1KB4940
(Linear) (Six-base) MAPSORT of: KIBB4940.seq Check: 3530 from: 1 to: 1437
KIBB4940.orf
With 182 enzymes: SgfI *
February 28, 2007 11:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 100 448 1256 1437
Size: 100 348 808 181
Fragments arranged by size:
808 348 181 100
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 712 1437
Size: 712 725
AhdI GACnn_n'nnGTC
Cuts at: 0 101 956 1437
Size: 101 855 481
Fragments arranged by size:
855 481 101
AleI CACnn'nnGTG
Cuts at: 0 1190 1262 1437
Size: 1190 72 175
Fragments arranged by size:
1190 175 72
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 703 1437
Size: 703 734
AlwNI CAG_nnn'CTG
Cuts at: 0 986 1437
Size: 986 451
ApaI G_GGCC'C
Cuts at: 0 1037 1437
Size: 1037 400
ApaLI G'TGCA_C
Cuts at: 0 852 1437
Size: 852 585
ApoI r'AATT_y
Cuts at: 0 1306 1437
Size: 1306 131
AvaI C'yCGr_G
Cuts at: 0 477 585 1029 1437
Size: 477 108 444 408
Fragments arranged by size:
477 444 408 108
BanI G'GyrC_C
Cuts at: 0 112 264 486 789 1355 1437
Size: 112 152 222 303 566 82
Fragments arranged by size:
566 303 222 152 112 82
BanII G_rGCy'C
Cuts at: 0 143 583 608 1037 1103 1136 1437
Size: 143 440 25 429 66 33 301
Fragments arranged by size:
440 429 301 143 66 33 25
BbeI G_GCGC'C
Cuts at: 0 268 490 793 1437
Size: 268 222 303 644
Fragments arranged by size:
644 303 268 222
BbsI GAAGACnn'nnnn_
Cuts at: 0 100 1158 1437
Size: 100 1058 279
Fragments arranged by size:
1058 279 100
BglI GCCn_nnn'nGGC
Cuts at: 0 241 587 767 1437
Size: 241 346 180 670
Fragments arranged by size:
670 346 241 180
BlpI GC'TnA_GC
Cuts at: 0 703 973 1437
Size: 703 270 464
Fragments arranged by size:
703 464 270
Bme1580I G_kGCm'C
Cuts at: 0 117 823 856 1037 1358 1437
Size: 117 706 33 181 321 79
Fragments arranged by size:
706 321 181 117 79 33
BmgBI CAC'GTC
Cuts at: 0 576 1437
Size: 576 861
BmrI ACTGGGnnnn_n'
Cuts at: 0 160 1296 1437
Size: 160 1136 141
Fragments arranged by size:
1136 160 141
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 159 529 861 1437
Size: 159 370 332 576
Fragments arranged by size:
576 370 332 159
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 241 1437
Size: 241 1196
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 982 1222 1437
Size: 982 240 215
Fragments arranged by size:
982 240 215
BsaI GGTCTCn'nnnn_
Cuts at: 0 277 849 1437
Size: 277 572 588
Fragments arranged by size:
588 572 277
BsaAI yAC'GTr
Cuts at: 0 818 1437
Size: 818 619
BsaHI Gr'CG_yC
Cuts at: 0 265 487 697 790 1437
Size: 265 222 210 93 647
Fragments arranged by size:
647 265 222 210 93
BsaWI w'CCGG_w
Cuts at: 0 348 1437
Size: 348 1089
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 330 360 1437
Size: 330 30 1077
Fragments arranged by size:
1077 330 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 151 304 381 502 1144 1437
Size: 151 153 77 121 642 293
Fragments arranged by size:
642 293 153 151 121 77
BseYI C'CCAG_C
Cuts at: 0 934 1437
Size: 934 503
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 887 1437
Size: 887 550
BsiEI CG_ry'CG
Cuts at: 0 180 206 898 1159 1386 1437
Size: 180 26 692 261 227 51
Fragments arranged by size:
692 261 227 180 51 26
BsiHKAI G_wGCw'C
Cuts at: 0 143 608 856 1103 1437
Size: 143 465 248 247 334
Fragments arranged by size:
465 334 248 247 143
Bsp1286I G_dGCh'C
Cuts at: 0 117 143 583 608 823 856 1037 1103
Size: 117 26 440 25 215 33 181 66
Cuts at: 1103 1136 1358 1437
Size: 33 222 79
Fragments arranged by size:
440 222 215 181 117 79 66 33
33 26 25
BspEI T'CCGG_A
Cuts at: 0 348 1437
Size: 348 1089
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 100 448 1256 1437
Size: 100 348 808 181
Fragments arranged by size:
808 348 181 100
BsrBI CCG'CTC
Cuts at: 0 227 532 1086 1316 1437
Size: 227 305 554 230 121
Fragments arranged by size:
554 305 230 227 121
BsrDI GCAATG_nn'
Cuts at: 0 891 1437
Size: 891 546
BsrFI r'CCGG_y
Cuts at: 0 235 893 897 1437
Size: 235 658 4 540
Fragments arranged by size:
658 540 235 4
BsrGI T'GTAC_A
Cuts at: 0 632 1437
Size: 632 805
BssHII G'CGCG_C
Cuts at: 0 428 1437
Size: 428 1009
BstXI CCAn_nnnn'nTGG
Cuts at: 0 219 1437
Size: 219 1218
BtgI C'CryG_G
Cuts at: 0 206 1437
Size: 206 1231
BtsI GCAGTG_nn'
Cuts at: 0 97 1437
Size: 97 1340
DraIII CAC_nnn'GTG
Cuts at: 0 780 1437
Size: 780 657
DrdI GACnn_nn'nnGTC
Cuts at: 0 31 1437
Size: 31 1406
EaeI y'GGCC_r
Cuts at: 0 177 209 359 599 613 888 895 1437
Size: 177 32 150 240 14 275 7 542
Fragments arranged by size:
542 275 240 177 150 32 14 7
EagI C'GGCC_G
Cuts at: 0 177 895 1437
Size: 177 718 542
Fragments arranged by size:
718 542 177
EarI CTCTTCn'nnn_
Cuts at: 0 399 852 1420 1437
Size: 399 453 568 17
Fragments arranged by size:
568 453 399 17
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 245 518 577 629 1437
Size: 245 273 59 52 808
Fragments arranged by size:
808 273 245 59 52
EcoICRI GAG'CTC
Cuts at: 0 141 606 1101 1437
Size: 141 465 495 336
Fragments arranged by size:
495 465 336 141
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 241 712 1437
Size: 241 471 725
Fragments arranged by size:
725 471 241
EcoO109I rG'GnC_Cy
Cuts at: 0 1033 1437
Size: 1033 404
HaeII r_GCGC'y
Cuts at: 0 268 490 710 793 869 1437
Size: 268 222 220 83 76 568
Fragments arranged by size:
568 268 222 220 83 76
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 87 119 330 362 863 895 942 974
Size: 87 32 211 32 501 32 47 32
Cuts at: 974 1218 1250 1437
Size: 244 32 187
Fragments arranged by size:
501 244 211 187 87 47 32 32
32 32 32
KasI G'GCGC_C
Cuts at: 0 264 486 789 1437
Size: 264 222 303 648
Fragments arranged by size:
648 303 264 222
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 565 1382 1437
Size: 565 817 55
Fragments arranged by size:
817 565 55
MscI TGG'CCA
Cuts at: 0 211 890 1437
Size: 211 679 547
Fragments arranged by size:
679 547 211
MslI CAynn'nnrTG
Cuts at: 0 1190 1262 1437
Size: 1190 72 175
Fragments arranged by size:
1190 175 72
MspA1I CmG'CkG
Cuts at: 0 128 416 1437
Size: 128 288 1021
Fragments arranged by size:
1021 288 128
NaeI GCC'GGC
Cuts at: 0 899 1437
Size: 899 538
NarI GG'CG_CC
Cuts at: 0 265 487 790 1437
Size: 265 222 303 647
Fragments arranged by size:
647 303 265 222
NgoMIV G'CCGG_C
Cuts at: 0 897 1437
Size: 897 540
NspI r_CATG'y
Cuts at: 0 1125 1437
Size: 1125 312
PasI CC'CwG_GG
Cuts at: 0 918 954 1437
Size: 918 36 483
Fragments arranged by size:
918 483 36
PflMI CCAn_nnn'nTGG
Cuts at: 0 780 1437
Size: 780 657
PfoI T'CCnGG_A
Cuts at: 0 305 1437
Size: 305 1132
PmlI CAC'GTG
Cuts at: 0 818 1437
Size: 818 619
PspOMI G'GGCC_C
Cuts at: 0 1033 1437
Size: 1033 404
PstI C_TGCA'G
Cuts at: 0 445 1437
Size: 445 992
PvuI CG_AT'CG
Cuts at: 0 1159 1437
Size: 1159 278
SacI G_AGCT'C
Cuts at: 0 143 608 1103 1437
Size: 143 465 495 334
Fragments arranged by size:
495 465 334 143
SfcI C'TryA_G
Cuts at: 0 441 1437
Size: 441 996
SfoI GGC'GCC
Cuts at: 0 266 488 791 1437
Size: 266 222 303 646
Fragments arranged by size:
646 303 266 222
SmaI CCC'GGG
Cuts at: 0 479 1031 1437
Size: 479 552 406
Fragments arranged by size:
552 479 406
SmlI C'TyrA_G
Cuts at: 0 961 1237 1437
Size: 961 276 200
Fragments arranged by size:
961 276 200
SphI G_CATG'C
Cuts at: 0 1125 1437
Size: 1125 312
StuI AGG'CCT
Cuts at: 0 509 1437
Size: 509 928
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 301 726 871 1198 1400 1437
Size: 301 425 145 327 202 37
Fragments arranged by size:
425 327 301 202 145 37
TatI w'GTAC_w
Cuts at: 0 632 1437
Size: 632 805
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 896 928 1437
Size: 896 32 509
Fragments arranged by size:
896 509 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 865 1437
Size: 865 572
XmaI C'CCGG_G
Cuts at: 0 477 1029 1437
Size: 477 552 408
Fragments arranged by size:
552 477 408
Enzymes that do cut:
AarI AcuI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AvaI BanI BanII BbeI BbsI BglI BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BstXI BtgI BtsI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I HaeII Hin4I KasI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PasI
PflMI PfoI PmlI PspOMI PstI PvuI SacI SfcI
SfoI SmaI SmlI SphI StuI TaqII TatI TstI
XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AloI AscI AseI AsiSI AvrII BaeI BamHI BbvCI
BcgI BciVI BclI BfrBI BglII BmtI Bpu10I BsaBI
BsiWI BsmI BsmBI BspHI BssSI BstAPI BstBI BstEII
BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII
HpaI KpnI MfeI MluI NcoI NdeI NheI NotI
NruI NsiI PacI PciI PmeI PpiI PpuMI PshAI
PsiI PspXI PsrI PvuII RsrII SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI
SpeI SrfI SspI StyI SwaI Tth111I XbaI XhoI
XmnI ZraI