Restriction map for pF1KB4877
##################################
# NO CUT ENZYMES (  107 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflII       
AgeI        AhdI        Alw44I      AocI        Aor51HI     
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AviII       AvrII       
BamHI       BbrPI       BcgI        BciVI       BclI        
BfrI        BlnI        BlpI        Bpu1102I    BsaAI       
BsaBI       BsaWI       BseAI       BsiWI       Bsp1407I    
BspDI       BspEI       BspHI       BsrDI       BsrGI       
BssHII      BstAPI      BstBI       BstEII      BstPI       
Bsu36I      CelII       ClaI        CpoI        DraIII      
Eam1105I    Eco47III    Eco81I      EcoO65I     EcoRI       
EcoRV       EcoT22I     FbaI        FseI        FspI        
HincII      HindII      HindIII     HpaI        KpnI        
MamI        MfeI        MluI        MroI        MunI        
NdeI        NheI        NotI        NruI        NsiI        
NspV        PacI        PflFI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RcaI        RsrII       
SalI        SbfI        ScaI        SexAI       SfcI        
SfiI        SfuI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        SwaI        Tth111I     
XbaI        XmnI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AccI         1359  GTATAC               GT^[AC][GT]AC
AfaI          720  GTAC                 GT^AC
AflIII       1162  ACATGT               A^C[GA][CT]GT
ApaI          354  GGGCCC               GGGCC^C
BalI         2055  TGGCCA               TGG^CCA
BanI          594  GGCGCC               G^G[CT][GA]CC
BbeI          594  GGCGCC               GGCGC^C
BbvCI         757  CCTCAGC              CCTCAGC(-5/-2)
BglII        1221  AGATCT               A^GATCT
BsaI           85  GGTCTC               GGTCTC(1/5)
BsgI         1301  GTGCAG               GTGCAG(16/14)
BsmBI         856  CGTCTC               CGTCTC(1/5)
BsmI         2267  GAATGC               GAATGC(1/-1)
BspLU11I     1162  ACATGT               A^CATGT
BspMI        1561  GCAGGT               ACCTGC(4/8)
BsrBI         290  CCGCTC               CCGCTC(-3/-3)
Bst1107I     1359  GTATAC               GTA^TAC
BstXI        1623  CCAGTGTCTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI        1221  AGATCT               [GA]^GATC[CT]
BstZ17I      1359  GTATAC               GTA^TAC
DraI          838  TTTAAA               TTT^AAA
DraII        1410  GGGGCCT              [GA]G^G[ACGT]CC[CT]
DrdI         1686  GACAAATGTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I     1410  GGGGCCT              [GA]G^G[ACGT]CC[CT]
KasI          594  GGCGCC               G^GCGCC
MflI         1221  AGATCT               [GA]^GATC[CT]
MluNI        2055  TGGCCA               TGG^CCA
MscI         2055  TGGCCA               TGG^CCA
NarI          594  GGCGCC               GG^CGCC
PaeR7I        220  CTCGAG               C^TCGAG
PflMI        1459  CCAGGAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI          79  GACAAGGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsaI          720  GTAC                 GT^AC
SfaNI         916  GCATC                GCATC(5/9)
SfoI          594  GGCGCC               GGC^GCC
SgrAI         494  CGCCGGCG             C[GA]^CCGG[CT]G
StuI          216  AGGCCT               AGG^CCT
Van91I       1459  CCAGGAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI         2058  CCATAGAGGACGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI          220  CTCGAG               C^TCGAG
XhoII        1221  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   32 enzymes) #
###################################

AcyI           68  GACGCC               G[GA]^CG[CT]C
              594  GGCGCC              
AlwI         1389  GGATC                GGATC(4/5)
             1680  GGATC               
AlwNI        1460  CAGGAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1583  CAGATACTG           
AvaII        1402  GGACC                G^G[AT]CC
             1839  GGTCC               
BbsI          385  GAAGAC               GAAGAC(2/6)
             1774  GAAGAC              
BfaI          178  CTAG                 C^TAG
              316  CTAG                
BglI          334  GCCGCGGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              538  GCCAAGGAGGC         
BpmI          112  CTCCAG               CTGGAG(16/14)
              905  CTGGAG              
BpuAI         385  GAAGAC               GAAGAC(2/6)
             1774  GAAGAC              
BsaHI          68  GACGCC               G[GA]^CG[CT]C
              594  GGCGCC              
BsiEI         236  CGGCCG               CG[GA][CT]^CG
              344  CGGCCG              
BsrFI         238  GCCGGC               [GA]^CCGG[CT]
              495  GCCGGC              
BssSI         689  CACGAG               CACGAG(-5/-1)
             2204  CTCGTG              
BstF5I        917  CATCC                GGATG(2/0)
             1368  CATCC               
Cfr10I        238  GCCGGC               [GA]^CCGG[CT]
              495  GCCGGC              
EagI          236  CGGCCG               C^GGCCG
              344  CGGCCG              
EclXI         236  CGGCCG               C^GGCCG
              344  CGGCCG              
Eco52I        236  CGGCCG               C^GGCCG
              344  CGGCCG              
EcoNI         219  CCTCGAGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1866  CCTCCCAGAGG         
FokI          917  CATCC                GGATG(9/13)
             1368  CATCC               
HaeII         203  GGCGCT               [GA]GCGC^[CT]
              594  GGCGCC              
HgaI           68  GACGC                GACGC(5/10)
              501  GACGC               
Hin1I          68  GACGCC               G[GA]^CG[CT]C
              594  GGCGCC              
MaeI          178  CTAG                 C^TAG
              316  CTAG                
MaeII        1376  ACGT                 A^CGT
             2067  ACGT                
NaeI          238  GCCGGC               GCC^GGC
              495  GCCGGC              
NcoI          155  CCATGG               C^CATGG
             2386  CCATGG              
NgoMIV        238  GCCGGC               G^CCGGC
              495  GCCGGC              
SmaI          163  CCCGGG               CCC^GGG
              601  CCCGGG              
Tsp45I        586  GTGAC                ^GT[GC]AC
             1032  GTCAC               
XmaCI         163  CCCGGG               C^CCGGG
              601  CCCGGG              
XmaI          163  CCCGGG               C^CCGGG
              601  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   78 enzymes) #
#########################################

AccII       AciI        AcsI        AluI        ApoI        
AspHI       AvaI        BanII       BbvI        BcnI        
BmyI        BsaJI       BseRI       BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1286I    
BsrI        BssKI       BstNI       BstUI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DsaI        
EaeI        EarI        EcoRII      EcoT14I     FauI        
Fnu4HI      HaeIII      HapII       HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        Ksp632I     
KspI        MaeIII      MboI        MboII       MnlI        
MseI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NciI        NdeII       NlaIII      
NlaIV       NspI        PleI        SacI        SacII       
SapI        Sau3AI      Sau96I      ScrFI       SmlI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp509I     TspRI       TthHB8I     
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