Restriction map for pF1KB4877
 (Linear) (Six-base) MAPSORT of: KIBB4877.seq  Check: 6222  from: 1  to: 2418



KIBB4877.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:26  ..

AccI GT'mk_AC

Cuts at:      0    1360    2418 
   Size:       1360    1058
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      52    1142    1373    1436    1897    2283    2418 
   Size:         52    1090     231      63     461     386     135

  Fragments arranged by size: 

                1090     461     386     231     135      63      52
 
AflIII A'CryG_T

Cuts at:      0    1162    2418 
   Size:       1162    1256
 
AleI CACnn'nnGTG

Cuts at:      0    1298    2418 
   Size:       1298    1120
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1823    1855    2418 
   Size:       1823      32     563

  Fragments arranged by size: 

                1823     563      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1465    1588    2418 
   Size:       1465     123     830

  Fragments arranged by size: 

                1465     830     123
 
ApaI G_GGCC'C

Cuts at:      0     358    2418 
   Size:        358    2060
 
ApoI r'AATT_y

Cuts at:      0    1069    1309    1636    1668    2094    2418 
   Size:       1069     240     327      32     426     324

  Fragments arranged by size: 

                1069     426     327     324     240      32
 
AvaI C'yCGr_G

Cuts at:      0     163     207     220     351     433     601    2418 
   Size:        163      44      13     131      82     168    1817

  Fragments arranged by size: 

                1817     168     163     131      82      44      13
 
BanI G'GyrC_C

Cuts at:      0     594    2418 
   Size:        594    1824
 
BanII G_rGCy'C

Cuts at:      0      59     101     164     358     434    1620    2112    2418 
   Size:         59      42      63     194      76    1186     492     306

  Fragments arranged by size: 

                1186     492     306     194      76      63      59      42
            
 
BbeI G_GCGC'C

Cuts at:      0     598    2418 
   Size:        598    1820
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     392    1781    2418 
   Size:        392    1389     637

  Fragments arranged by size: 

                1389     637     392
 
BbvCI CC'TCA_GC

Cuts at:      0     758    2418 
   Size:        758    1660
 
BglI GCCn_nnn'nGGC

Cuts at:      0     340     544    2418 
   Size:        340     204    1874

  Fragments arranged by size: 

                1874     340     204
 
BglII A'GATC_T

Cuts at:      0    1221    2418 
   Size:       1221    1197
 
Bme1580I G_kGCm'C

Cuts at:      0     358     473    2211    2418 
   Size:        358     115    1738     207

  Fragments arranged by size: 

                1738     358     207     115
 
BmgBI CAC'GTC

Cuts at:      0    2068    2418 
   Size:       2068     350
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1617    2418 
   Size:       1617     801
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     235     396     874    2418 
   Size:        235     161     478    1544

  Fragments arranged by size: 

                1544     478     235     161
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      97     926    2418 
   Size:         97     829    1492

  Fragments arranged by size: 

                1492     829      97
 
Bpu10I CC'TnA_GC

Cuts at:      0     758    2418 
   Size:        758    1660
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      43      73    2418 
   Size:         43      30    2345

  Fragments arranged by size: 

                2345      43      30
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      91    2418 
   Size:         91    2327
 
BsaHI Gr'CG_yC

Cuts at:      0      69     595    2418 
   Size:         69     526    1823

  Fragments arranged by size: 

                1823     526      69
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      67     148     268     271     286     289     337     379 
   Size:         67      81     120       3      15       3      48      42

Cuts at:    379     382     385     746    1725    2117    2120    2418 
   Size:          3       3     361     979     392       3     298

  Fragments arranged by size: 

                 979     392     361     298     120      81      67      48
                  42      15       3       3       3       3       3
 
BseYI C'CCAG_C

Cuts at:      0     199    2418 
   Size:        199    2219
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1322    2418 
   Size:       1322    1096
 
BsiEI CG_ry'CG

Cuts at:      0     239     347    2418 
   Size:        239     108    2071

  Fragments arranged by size: 

                2071     239     108
 
BsiHKAI G_wGCw'C

Cuts at:      0      59     101     434    2418 
   Size:         59      42     333    1984

  Fragments arranged by size: 

                1984     333      59      42
 
BsmI GAATG_Cn'

Cuts at:      0    2273    2418 
   Size:       2273     145
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     862    2418 
   Size:        862    1556
 
Bsp1286I G_dGCh'C

Cuts at:      0      59     101     164     358     434     473    1620    2112 
   Size:         59      42      63     194      76      39    1147     492

Cuts at:   2112    2211    2418 
   Size:         99     207

  Fragments arranged by size: 

                1147     492     207     194      99      76      63      59
                  42      39
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1552    2418 
   Size:       1552     866
 
BsrBI CCG'CTC

Cuts at:      0     292    2418 
   Size:        292    2126
 
BsrFI r'CCGG_y

Cuts at:      0     238     495    2418 
   Size:        238     257    1923

  Fragments arranged by size: 

                1923     257     238
 
BssSI C'ACGA_G

Cuts at:      0     689    2204    2418 
   Size:        689    1515     214

  Fragments arranged by size: 

                1515     689     214
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1630    2418 
   Size:       1630     788
 
BstYI r'GATC_y

Cuts at:      0    1221    2418 
   Size:       1221    1197
 
BstZ17I GTA'TAC

Cuts at:      0    1361    2418 
   Size:       1361    1057
 
BtgI C'CryG_G

Cuts at:      0     155     233     335     548     705    2226    2386    2418 
   Size:        155      78     102     213     157    1521     160      32

  Fragments arranged by size: 

                1521     213     160     157     155     102      78      32
            
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     260    2418 
   Size:        260    2158
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    2067    2102    2244    2279    2418 
   Size:       2067      35     142      35     139

  Fragments arranged by size: 

                2067     142     139      35      35
 
DraI TTT'AAA

Cuts at:      0     840    2418 
   Size:        840    1578
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1692    2418 
   Size:       1692     726
 
EaeI y'GGCC_r

Cuts at:      0     236     344     702    1267    2055    2223    2418 
   Size:        236     108     358     565     788     168     195

  Fragments arranged by size: 

                 788     565     358     236     195     168     108
 
EagI C'GGCC_G

Cuts at:      0     236     344    2418 
   Size:        236     108    2074

  Fragments arranged by size: 

                2074     236     108
 
EarI CTCTTCn'nnn_

Cuts at:      0     245     257     263     437     550     722     906    1349 
   Size:        245      12       6     174     113     172     184     443

Cuts at:   1349    1412    1976    2418 
   Size:         63     564     442

  Fragments arranged by size: 

                 564     443     442     245     184     174     172     113
                  63      12       6
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     674    2141    2418 
   Size:        674    1467     277

  Fragments arranged by size: 

                1467     674     277
 
EcoICRI GAG'CTC

Cuts at:      0      57      99     432    2418 
   Size:         57      42     333    1986

  Fragments arranged by size: 

                1986     333      57      42
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      52      97     926    1142    1373    1436    1897    2283 
   Size:         52      45     829     216     231      63     461     386

Cuts at:   2283    2418 
   Size:        135

  Fragments arranged by size: 

                 829     461     386     231     216     135      63      52
                  45
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     223    1870    2418 
   Size:        223    1647     548

  Fragments arranged by size: 

                1647     548     223
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1411    2418 
   Size:       1411    1007
 
HaeII r_GCGC'y

Cuts at:      0     207     598    2418 
   Size:        207     391    1820

  Fragments arranged by size: 

                1820     391     207
 
KasI G'GCGC_C

Cuts at:      0     594    2418 
   Size:        594    1824
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      20    2418 
   Size:         20    2398
 
MscI TGG'CCA

Cuts at:      0    2057    2418 
   Size:       2057     361
 
MslI CAynn'nnrTG

Cuts at:      0     704     791    1298    2418 
   Size:        704      87     507    1120

  Fragments arranged by size: 

                1120     704     507      87
 
MspA1I CmG'CkG

Cuts at:      0     199     235     337     550     563    2418 
   Size:        199      36     102     213      13    1855

  Fragments arranged by size: 

                1855     213     199     102      36      13
 
NaeI GCC'GGC

Cuts at:      0     240     497    2418 
   Size:        240     257    1921

  Fragments arranged by size: 

                1921     257     240
 
NarI GG'CG_CC

Cuts at:      0     595    2418 
   Size:        595    1823
 
NcoI C'CATG_G

Cuts at:      0     155    2386    2418 
   Size:        155    2231      32

  Fragments arranged by size: 

                2231     155      32
 
NgoMIV G'CCGG_C

Cuts at:      0     238     495    2418 
   Size:        238     257    1923

  Fragments arranged by size: 

                1923     257     238
 
NspI r_CATG'y

Cuts at:      0    1109    1166    1285    1495    1837    2151    2418 
   Size:       1109      57     119     210     342     314     267

  Fragments arranged by size: 

                1109     342     314     267     210     119      57
 
PasI CC'CwG_GG

Cuts at:      0    2113    2418 
   Size:       2113     305
 
PciI A'CATG_T

Cuts at:      0    1162    2418 
   Size:       1162    1256
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1465    2418 
   Size:       1465     953
 
PfoI T'CCnGG_A

Cuts at:      0     413    2418 
   Size:        413    2005
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1984    2016    2418 
   Size:       1984      32     402

  Fragments arranged by size: 

                1984     402      32
 
PshAI GACnn'nnGTC

Cuts at:      0      83    2418 
   Size:         83    2335
 
PsiI TTA'TAA

Cuts at:      0     821    2418 
   Size:        821    1597
 
PspOMI G'GGCC_C

Cuts at:      0     354    2418 
   Size:        354    2064
 
PspXI vC'TCGA_Gb

Cuts at:      0     220    2418 
   Size:        220    2198
 
SacI G_AGCT'C

Cuts at:      0      59     101     434    2418 
   Size:         59      42     333    1984

  Fragments arranged by size: 

                1984     333      59      42
 
SacII CC_GC'GG

Cuts at:      0     236     338     551    2418 
   Size:        236     102     213    1867

  Fragments arranged by size: 

                1867     236     213     102
 
SapI GCTCTTCn'nnn_

Cuts at:      0     550     906    1976    2418 
   Size:        550     356    1070     442

  Fragments arranged by size: 

                1070     550     442     356
 
SfoI GGC'GCC

Cuts at:      0     596    2418 
   Size:        596    1822
 
SgrAI Cr'CCGG_yG

Cuts at:      0     495    2418 
   Size:        495    1923
 
SmaI CCC'GGG

Cuts at:      0     165     603    2418 
   Size:        165     438    1815

  Fragments arranged by size: 

                1815     438     165
 
SmlI C'TyrA_G

Cuts at:      0      58      88     220    2418 
   Size:         58      30     132    2198

  Fragments arranged by size: 

                2198     132      58      30
 
StuI AGG'CCT

Cuts at:      0     218    2418 
   Size:        218    2200
 
StyI C'CwwG_G

Cuts at:      0     155     539    2386    2406    2418 
   Size:        155     384    1847      20      12

  Fragments arranged by size: 

                1847     384     155      20      12
 
TatI w'GTAC_w

Cuts at:      0     719    2418 
   Size:        719    1699
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    2065    2418 
   Size:       2065     353
 
XhoI C'TCGA_G

Cuts at:      0     220    2418 
   Size:        220    2198
 
XmaI C'CCGG_G

Cuts at:      0     163     601    2418 
   Size:        163     438    1817

  Fragments arranged by size: 

                1817     438     163
 

 Enzymes that do cut:

     AccI     AcuI   AflIII     AleI     AloI    AlwNI     ApaI     ApoI
     AvaI     BanI    BanII     BbeI     BbsI    BbvCI     BglI    BglII
 Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaHI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI
 Bsp1286I    BspMI    BsrBI    BsrFI    BssSI    BstXI    BstYI  BstZ17I
     BtgI    BtgZI    CspCI     DraI     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    HaeII     KasI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI
     PasI     PciI    PflMI     PfoI     PpiI    PshAI     PsiI   PspOMI
    PspXI     SacI    SacII     SapI     SfoI    SgrAI     SmaI     SmlI
     StuI     StyI     TatI     XcmI     XhoI     XmaI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AlfI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI
     BcgI    BciVI     BclI    BfrBI     BlpI     BmtI    BsaAI    BsaBI
    BsaWI    BsaXI    BsiWI    BspEI    BspHI    BsrDI    BsrGI   BssHII
   BstAPI    BstBI   BstEII   Bsu36I     BtsI     ClaI   DraIII    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    Hin4I   HincII  HindIII
     HpaI     KpnI     MfeI     MluI     NdeI     NheI     NotI     NruI
     NsiI     PacI     PmeI     PmlI    PpuMI     PsrI     PstI     PvuI
    PvuII    RsrII     SalI    SanDI     SbfI     ScaI    SexAI     SfcI
     SfiI     SgfI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI
    TaqII     TstI  Tth111I     XbaI     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com