Restriction map for pF1KB4875
##################################
# NO CUT ENZYMES (   82 enzymes) #
##################################

AatII       AccI        AclI        AcsI        AflII       
AgeI        AhdI        Alw44I      ApaLI       ApoI        
AseI        AsnI        AspEI       AspI        AvrII       
BamHI       BbsI        BciVI       BclI        BfrI        
BglII       BlnI        BpuAI       BsaBI       BsiWI       
BsmI        BspDI       BspHI       BspLU11I    BsrDI       
Bst1107I    BstBI       BstZ17I     ClaI        CpoI        
DraI        Eam1105I    EcoRI       EcoRV       EcoT22I     
FbaI        HincII      HindII      HindIII     HpaI        
MamI        MluI        MseI        NdeI        NheI        
NotI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmeI        PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SalI        SbfI        ScaI        SexAI       SfiI        
SfuI        SgrAI       SnaBI       SpeI        SphI        
Sse8387I    SspI        SwaI        Tru9I       Tth111I     
XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   26 enzymes) #
###################################

Acc65I        948  GGTACC               G^GTACC
AocI         2875  CCTAAGG              CC^T[ACGT]AGG
Asp700I       687  GAACTTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I       948  GGTACC               G^GTACC
AviII         152  TGCGCA               TGC^GCA
BbvCI        2691  GCTGAGG              CCTCAGC(-5/-2)
BsmBI        2152  GAGACG               CGTCTC(1/5)
BssSI        1204  CACGAG               CACGAG(-5/-1)
Bsu36I       2875  CCTAAGG              CC^T[ACGT]AGG
DraIII        815  CACTCCGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         1632  GACCACTACGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I       2875  CCTAAGG              CC^T[ACGT]AGG
FspI          152  TGCGCA               TGC^GCA
KpnI          948  GGTACC               GGTAC^C
KspI         1956  CCGCGG               CCGC^GG
MfeI         1890  CAATTG               C^AATTG
MunI         1890  CAATTG               C^AATTG
NcoI         1577  CCATGG               C^CATGG
NruI         1902  TCGCGA               TCG^CGA
SacI          502  GAGCTC               GAGCT^C
SacII        1956  CCGCGG               CCGC^GG
SapI          261  GCTCTTC              GCTCTTC(1/4)
SmlI          331  CTTGAG               C^T[CT][GA]AG
StuI          525  AGGCCT               AGG^CCT
Tsp509I      1891  AATT                 ^AATT
XmnI          687  GAACTTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AccIII        809  TCCGGA               T^CCGGA
             1268  TCCGGA              
AflIII       1220  ACGTGT               A^C[GA][CT]GT
             1367  ACGTGT              
Aor51HI      1530  AGCGCT               AGC^GCT
             2135  AGCGCT              
AscI         2019  GGCGCGCC             GG^CGCGCC
             2175  GGCGCGCC            
BcgI         1967  CGATCACGCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             2223  CGAGGCCGCTGC        
BsaWI         809  TCCGGA               [AT]^CCGG[AT]
             1268  TCCGGA              
BseAI         809  TCCGGA               T^CCGGA
             1268  TCCGGA              
Bsp1407I     1049  TGTACA               T^GTACA
             2321  TGTACA              
BspEI         809  TCCGGA               T^CCGGA
             1268  TCCGGA              
BsrGI        1049  TGTACA               T^GTACA
             2321  TGTACA              
BstEII       1629  GGTGACC              G^GT[ACGT]ACC
             2391  GGTGACC             
BstPI        1629  GGTGACC              G^GT[ACGT]ACC
             2391  GGTGACC             
BstXI        1311  CCAGGAGCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3092  CCAAGCAGCTGG        
BstYI        2364  AGATCC               [GA]^GATC[CT]
             2466  GGATCT              
EarI          262  CTCTTC               CTCTTC(1/4)
              226  GAAGAG              
Eco47III     1530  AGCGCT               AGC^GCT
             2135  AGCGCT              
EcoO65I      1629  GGTGACC              G^GT[ACGT]ACC
             2391  GGTGACC             
FseI         1551  GGCCGGCC             GGCCGG^CC
             2293  GGCCGGCC            
Ksp632I       262  CTCTTC               CTCTTC(1/4)
              226  GAAGAG              
MflI         2364  AGATCC               [GA]^GATC[CT]
             2466  GGATCT              
MroI          809  TCCGGA               T^CCGGA
             1268  TCCGGA              
SmaI         1765  CCCGGG               CCC^GGG
             2170  CCCGGG              
SspBI        1049  TGTACA               T^GTACA
             2321  TGTACA              
TfiI         1162  GATTC                G^A[AT]TC
             2117  GATTC               
XhoII        2364  AGATCC               [GA]^GATC[CT]
             2466  GGATCT              
XmaCI        1765  CCCGGG               C^CCGGG
             2170  CCCGGG              
XmaI         1765  CCCGGG               C^CCGGG
             2170  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  123 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AlwNI       ApaI        AspHI       AvaI        
AvaII       BalI        BanI        BanII       BbeI        
BbrPI       BbvI        BcnI        BfaI        BglI        
BlpI        BmyI        BpmI        Bpu1102I    BsaAI       
BsaHI       BsaI        BsaJI       BseRI       BsgI        
BsiEI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BspMI       BsrBI       
BsrFI       BsrI        BssHII      BssKI       BstAPI      
BstF5I      BstNI       BstUI       Cac8I       CelII       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EagI        EclXI       
Eco52I      EcoNI       EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HinfI       HpaII       HphI        ItaI        KasI        
MaeI        MaeII       MaeIII      MboI        MboII       
MluNI       MnlI        MscI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NaeI        
NarI        NciI        NdeII       NgoMIV      NlaIII      
NlaIV       PflMI       PleI        PmaCI       PmlI        
PpuMI       PstI        PvuII       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SfoI        
StyI        TaqI        TseI        Tsp45I      TspRI       
TthHB8I     Van91I      XcmI        
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