Restriction map for pF1KB4875
 (Linear) (Six-base) MAPSORT of: KIBB4875.seq  Check: 3538  from: 1  to: 3126



KIBB4875.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:26  ..

Acc65I G'GTAC_C

Cuts at:      0     948    3126 
   Size:        948    2178
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     425    3126 
   Size:        425    2701
 
AfeI AGC'GCT

Cuts at:      0    1532    2137    3126 
   Size:       1532     605     989

  Fragments arranged by size: 

                1532     989     605
 
AflIII A'CryG_T

Cuts at:      0    1220    1367    3126 
   Size:       1220     147    1759

  Fragments arranged by size: 

                1759    1220     147
 
AleI CACnn'nnGTG

Cuts at:      0    1743    2128    3126 
   Size:       1743     385     998

  Fragments arranged by size: 

                1743     998     385
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1834    3126 
   Size:       1834    1292
 
AlwNI CAG_nnn'CTG

Cuts at:      0     313    1605    1938    2205    2241    2368    2421    2461 
   Size:        313    1292     333     267      36     127      53      40

Cuts at:   2461    2911    3126 
   Size:        450     215

  Fragments arranged by size: 

                1292     450     333     313     267     215     127      53
                  40      36
 
ApaI G_GGCC'C

Cuts at:      0     145     718     857    1143    1711    2361    3126 
   Size:        145     573     139     286     568     650     765

  Fragments arranged by size: 

                 765     650     573     568     286     145     139
 
AscI GG'CGCG_CC

Cuts at:      0    2020    2176    3126 
   Size:       2020     156     950

  Fragments arranged by size: 

                2020     950     156
 
AvaI C'yCGr_G

Cuts at:      0     505    1703    1765    2170    2221    2840    3126 
   Size:        505    1198      62     405      51     619     286

  Fragments arranged by size: 

                1198     619     505     405     286      62      51
 
BanI G'GyrC_C

Cuts at:      0     483     766     823     897     948    1688    1714    1761 
   Size:        483     283      57      74      51     740      26      47

Cuts at:   1761    1818    1863    2250    2682    3126 
   Size:         57      45     387     432     444

  Fragments arranged by size: 

                 740     483     444     432     387     283      74      57
                  57      51      47      45      26
 
BanII G_rGCy'C

Cuts at:      0      31     145     387     506     718     857     913     934 
   Size:         31     114     242     119     212     139      56      21

Cuts at:    934    1036    1143    1711    1738    1791    2361    3106    3126 
   Size:        102     107     568      27      53     570     745      20

  Fragments arranged by size: 

                 745     570     568     242     212     139     119     114
                 107     102      56      53      31      27      21      20
            
 
BbeI G_GCGC'C

Cuts at:      0     901    1692    1718    1765    1867    2254    2686    3126 
   Size:        901     791      26      47     102     387     432     440

  Fragments arranged by size: 

                 901     791     440     432     387     102      47      26
            
 
BbvCI CC'TCA_GC

Cuts at:      0    2692    3126 
   Size:       2692     434
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1956    1990    2212    2246    3126 
   Size:       1956      34     222      34     880

  Fragments arranged by size: 

                1956     880     222      34      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0      96     852    1025    1283    1786    1808    1832    2093 
   Size:         96     756     173     258     503      22      24     261

Cuts at:   2093    2155    2349    3121    3126 
   Size:         62     194     772       5

  Fragments arranged by size: 

                 772     756     503     261     258     194     173      96
                  62      24      22       5
 
BlpI GC'TnA_GC

Cuts at:      0     907     933     985    2900    3126 
   Size:        907      26      52    1915     226

  Fragments arranged by size: 

                1915     907     226      52      26
 
Bme1580I G_kGCm'C

Cuts at:      0     145     210     216     486     557     718     769     857 
   Size:        145      65       6     270      71     161      51      88

Cuts at:    857    1143    1389    1711    1823    2361    3126 
   Size:        286     246     322     112     538     765

  Fragments arranged by size: 

                 765     538     322     286     270     246     161     145
                 112      88      71      65      51       6
 
BmgBI CAC'GTC

Cuts at:      0     360    1908    3126 
   Size:        360    1548    1218

  Fragments arranged by size: 

                1548    1218     360
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    2539    2600    2648    3126 
   Size:       2539      61      48     478

  Fragments arranged by size: 

                2539     478      61      48
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     441    2823    2949    3126 
   Size:        441    2382     126     177

  Fragments arranged by size: 

                2382     441     177     126
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     610     646     907    2438    2794    2848    3025    3126 
   Size:        610      36     261    1531     356      54     177     101

  Fragments arranged by size: 

                1531     610     356     261     177     101      54      36
            
 
Bpu10I CC'TnA_GC

Cuts at:      0    1790    2692    2953    3126 
   Size:       1790     902     261     173

  Fragments arranged by size: 

                1790     902     261     173
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     352    3126 
   Size:        352    2774
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     398    2044    2864    2937    3126 
   Size:        398    1646     820      73     189

  Fragments arranged by size: 

                1646     820     398     189      73
 
BsaAI yAC'GTr

Cuts at:      0     894    1221    1368    2130    2713    3126 
   Size:        894     327     147     762     583     413

  Fragments arranged by size: 

                 894     762     583     413     327     147
 
BsaHI Gr'CG_yC

Cuts at:      0     898    1689    1715    1762    1864    2108    2251    2683 
   Size:        898     791      26      47     102     244     143     432

Cuts at:   2683    3126 
   Size:        443

  Fragments arranged by size: 

                 898     791     443     432     244     143     102      47
                  26
 
BsaWI w'CCGG_w

Cuts at:      0     809    1268    3126 
   Size:        809     459    1858

  Fragments arranged by size: 

                1858     809     459
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1033    1063    2767    2797    2821    2851    3065    3095 
   Size:       1033      30    1704      30      24      30     214      30

Cuts at:   3095    3126 
   Size:         31

  Fragments arranged by size: 

                1704    1033     214      31      30      30      30      30
                  24
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     607     757    1054    1057    1255    2491    2563    3022 
   Size:        607     150     297       3     198    1236      72     459

Cuts at:   3022    3025    3070    3126 
   Size:          3      45      56

  Fragments arranged by size: 

                1236     607     459     297     198     150      72      56
                  45       3       3
 
BseYI C'CCAG_C

Cuts at:      0     285     711     913    1098    1142    1830    2209    2234 
   Size:        285     426     202     185      44     688     379      25

Cuts at:   2234    3099    3126 
   Size:        865      27

  Fragments arranged by size: 

                 865     688     426     379     285     202     185      44
                  27      25
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     545     757    1383    2392    2407    2479    3086    3126 
   Size:        545     212     626    1009      15      72     607      40

  Fragments arranged by size: 

                1009     626     607     545     212      72      40      15
            
 
BsiEI CG_ry'CG

Cuts at:      0     578    1553    1844    1878    1956    2295    3126 
   Size:        578     975     291      34      78     339     831

  Fragments arranged by size: 

                 975     831     578     339     291      78      34
 
BsiHKAI G_wGCw'C

Cuts at:      0     506     632     893    3126 
   Size:        506     126     261    2233

  Fragments arranged by size: 

                2233     506     261     126
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    2146    3126 
   Size:       2146     980
 
Bsp1286I G_dGCh'C

Cuts at:      0      31     145     210     216     387     486     506     557 
   Size:         31     114      65       6     171      99      20      51

Cuts at:    557     632     718     769     857     893     913     934    1036 
   Size:         75      86      51      88      36      20      21     102

Cuts at:   1036    1143    1389    1711    1738    1791    1823    2361    3106 
   Size:        107     246     322      27      53      32     538     745

Cuts at:   3106    3126 
   Size:         20

  Fragments arranged by size: 

                 745     538     322     246     171     114     107     102
                  99      88      86      75      65      53      51      51
                  36      32      31      27      21      20      20      20
                   6
 
BspEI T'CCGG_A

Cuts at:      0     809    1268    3126 
   Size:        809     459    1858

  Fragments arranged by size: 

                1858     809     459
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     249     479    1613    2981    3126 
   Size:        249     230    1134    1368     145

  Fragments arranged by size: 

                1368    1134     249     230     145
 
BsrBI CCG'CTC

Cuts at:      0    1352    1799    2291    3126 
   Size:       1352     447     492     835

  Fragments arranged by size: 

                1352     835     492     447
 
BsrFI r'CCGG_y

Cuts at:      0      13    1071    1552    2294    2348    3038    3126 
   Size:         13    1058     481     742      54     690      88

  Fragments arranged by size: 

                1058     742     690     481      88      54      13
 
BsrGI T'GTAC_A

Cuts at:      0    1049    2321    3126 
   Size:       1049    1272     805

  Fragments arranged by size: 

                1272    1049     805
 
BssHII G'CGCG_C

Cuts at:      0    1561    1755    2020    2176    3126 
   Size:       1561     194     265     156     950

  Fragments arranged by size: 

                1561     950     265     194     156
 
BssSI C'ACGA_G

Cuts at:      0    1204    3126 
   Size:       1204    1922
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     369    1605    2421    3126 
   Size:        369    1236     816     705

  Fragments arranged by size: 

                1236     816     705     369
 
BstEII G'GTnAC_C

Cuts at:      0    1629    2391    3126 
   Size:       1629     762     735

  Fragments arranged by size: 

                1629     762     735
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1318    3099    3126 
   Size:       1318    1781      27

  Fragments arranged by size: 

                1781    1318      27
 
BstYI r'GATC_y

Cuts at:      0    2364    2466    3126 
   Size:       2364     102     660

  Fragments arranged by size: 

                2364     660     102
 
Bsu36I CC'TnA_GG

Cuts at:      0    2876    3126 
   Size:       2876     250
 
BtgI C'CryG_G

Cuts at:      0     819     859     952    1577    1950    1956    2003    2040 
   Size:        819      40      93     625     373       6      47      37

Cuts at:   2040    3126 
   Size:       1086

  Fragments arranged by size: 

                1086     819     625     373      93      47      40      37
                   6
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     179    1118    3126 
   Size:        179     939    2008

  Fragments arranged by size: 

                2008     939     179
 
BtsI GCAGTG_nn'

Cuts at:      0     280     562     731    2769    3126 
   Size:        280     282     169    2038     357

  Fragments arranged by size: 

                2038     357     282     280     169
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    2699    2734    3126 
   Size:       2699      35     392

  Fragments arranged by size: 

                2699     392      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     820    3126 
   Size:        820    2306
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1638    3126 
   Size:       1638    1488
 
EaeI y'GGCC_r

Cuts at:      0     575    1424    1523    1550    1554    1580    1616    1841 
   Size:        575     849      99      27       4      26      36     225

Cuts at:   1841    1875    1953    1995    2196    2292    2471    2579    3126 
   Size:         34      78      42     201      96     179     108     547

  Fragments arranged by size: 

                 849     575     547     225     201     179     108      99
                  96      78      42      36      34      27      26       4
            
 
EagI C'GGCC_G

Cuts at:      0     575    1550    1841    1875    1953    2292    3126 
   Size:        575     975     291      34      78     339     834

  Fragments arranged by size: 

                 975     834     575     339     291      78      34
 
EarI CTCTTCn'nnn_

Cuts at:      0     221     268    3126 
   Size:        221      47    2858

  Fragments arranged by size: 

                2858     221      47
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1493    3004    3067    3126 
   Size:       1493    1511      63      59

  Fragments arranged by size: 

                1511    1493      63      59
 
EcoICRI GAG'CTC

Cuts at:      0     504    3126 
   Size:        504    2622
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     425     610     646     907    2438    2794    2848    3025 
   Size:        425     185      36     261    1531     356      54     177

Cuts at:   3025    3126 
   Size:        101

  Fragments arranged by size: 

                1531     425     356     261     185     177     101      54
                  36
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    2632    2977    3007    3126 
   Size:       2632     345      30     119

  Fragments arranged by size: 

                2632     345     119      30
 
EcoO109I rG'GnC_Cy

Cuts at:      0      98     141     182     431     853     854    1483    1707 
   Size:         98      43      41     249     422       1     629     224

Cuts at:   1707    1988    2357    2358    2458    2541    2696    2768    2970 
   Size:        281     369       1     100      83     155      72     202

Cuts at:   2970    3126 
   Size:        156

  Fragments arranged by size: 

                 629     422     369     281     249     224     202     156
                 155     100      98      83      72      43      41       1
                   1
 
FseI GG_CCGG'CC

Cuts at:      0    1556    2298    3126 
   Size:       1556     742     828

  Fragments arranged by size: 

                1556     828     742
 
FspI TGC'GCA

Cuts at:      0     154    3126 
   Size:        154    2972
 
HaeII r_GCGC'y

Cuts at:      0     901    1278    1534    1692    1718    1765    1867    2068 
   Size:        901     377     256     158      26      47     102     201

Cuts at:   2068    2139    2254    2686    3126 
   Size:         71     115     432     440

  Fragments arranged by size: 

                 901     440     432     377     256     201     158     115
                 102      71      47      26
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    2821    2840    2853    2872    3126 
   Size:       2821      19      13      19     254

  Fragments arranged by size: 

                2821     254      19      19      13
 
KasI G'GCGC_C

Cuts at:      0     897    1688    1714    1761    1863    2250    2682    3126 
   Size:        897     791      26      47     102     387     432     444

  Fragments arranged by size: 

                 897     791     444     432     387     102      47      26
            
 
KpnI G_GTAC'C

Cuts at:      0     952    3126 
   Size:        952    2174
 
MfeI C'AATT_G

Cuts at:      0    1890    3126 
   Size:       1890    1236
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1017    2513    2732    3126 
   Size:       1017    1496     219     394

  Fragments arranged by size: 

                1496    1017     394     219
 
MscI TGG'CCA

Cuts at:      0    1525    1582    1618    2581    3126 
   Size:       1525      57      36     963     545

  Fragments arranged by size: 

                1525     963     545      57      36
 
MslI CAynn'nnrTG

Cuts at:      0    1743    1816    2128    3126 
   Size:       1743      73     312     998

  Fragments arranged by size: 

                1743     998     312      73
 
MspA1I CmG'CkG

Cuts at:      0     105     158     276     758     906     917    1732    1938 
   Size:        105      53     118     482     148      11     815     206

Cuts at:   1938    1958    2031    2230    2418    3099    3126 
   Size:         20      73     199     188     681      27

  Fragments arranged by size: 

                 815     681     482     206     199     188     148     118
                 105      73      53      27      20      11
 
NaeI GCC'GGC

Cuts at:      0      15    1554    2296    2350    3126 
   Size:         15    1539     742      54     776

  Fragments arranged by size: 

                1539     776     742      54      15
 
NarI GG'CG_CC

Cuts at:      0     898    1689    1715    1762    1864    2251    2683    3126 
   Size:        898     791      26      47     102     387     432     443

  Fragments arranged by size: 

                 898     791     443     432     387     102      47      26
            
 
NcoI C'CATG_G

Cuts at:      0    1577    3126 
   Size:       1577    1549
 
NgoMIV G'CCGG_C

Cuts at:      0      13    1552    2294    2348    3126 
   Size:         13    1539     742      54     778

  Fragments arranged by size: 

                1539     778     742      54      13
 
NruI TCG'CGA

Cuts at:      0    1904    3126 
   Size:       1904    1222
 
PasI CC'CwG_GG

Cuts at:      0    1677    3126 
   Size:       1677    1449
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1578    2004    2461    2866    2911    3126 
   Size:       1578     426     457     405      45     215

  Fragments arranged by size: 

                1578     457     426     405     215      45
 
PfoI T'CCnGG_A

Cuts at:      0     587    1310    3126 
   Size:        587     723    1816

  Fragments arranged by size: 

                1816     723     587
 
PmlI CAC'GTG

Cuts at:      0     894    1221    1368    2130    3126 
   Size:        894     327     147     762     996

  Fragments arranged by size: 

                 996     894     762     327     147
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     595     627    1198    1230    3126 
   Size:        595      32     571      32    1896

  Fragments arranged by size: 

                1896     595     571      32      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     431    1988    2768    2970    3126 
   Size:        431    1557     780     202     156

  Fragments arranged by size: 

                1557     780     431     202     156
 
PspOMI G'GGCC_C

Cuts at:      0     141     714     853    1139    1707    2357    3126 
   Size:        141     573     139     286     568     650     769

  Fragments arranged by size: 

                 769     650     573     568     286     141     139
 
PstI C_TGCA'G

Cuts at:      0     246    1727    2030    2339    2966    2978    3050    3126 
   Size:        246    1481     303     309     627      12      72      76

  Fragments arranged by size: 

                1481     627     309     303     246      76      72      12
            
 
PvuII CAG'CTG

Cuts at:      0     906     917    1938    2031    2418    3099    3126 
   Size:        906      11    1021      93     387     681      27

  Fragments arranged by size: 

                1021     906     681     387      93      27      11
 
SacI G_AGCT'C

Cuts at:      0     506    3126 
   Size:        506    2620
 
SacII CC_GC'GG

Cuts at:      0    1959    3126 
   Size:       1959    1167
 
SanDI GG'GwC_CC

Cuts at:      0     431    1988    2768    3126 
   Size:        431    1557     780     358

  Fragments arranged by size: 

                1557     780     431     358
 
SapI GCTCTTCn'nnn_

Cuts at:      0     268    3126 
   Size:        268    2858
 
SfcI C'TryA_G

Cuts at:      0     242     618    1416    1723    2026    2335    2962    2974 
   Size:        242     376     798     307     303     309     627      12

Cuts at:   2974    3046    3126 
   Size:         72      80

  Fragments arranged by size: 

                 798     627     376     309     307     303     242      80
                  72      12
 
SfoI GGC'GCC

Cuts at:      0     899    1690    1716    1763    1865    2252    2684    3126 
   Size:        899     791      26      47     102     387     432     442

  Fragments arranged by size: 

                 899     791     442     432     387     102      47      26
            
 
SmaI CCC'GGG

Cuts at:      0    1767    2172    3126 
   Size:       1767     405     954

  Fragments arranged by size: 

                1767     954     405
 
SmlI C'TyrA_G

Cuts at:      0     331    3126 
   Size:        331    2795
 
StuI AGG'CCT

Cuts at:      0     527    3126 
   Size:        527    2599
 
StyI C'CwwG_G

Cuts at:      0     874     944    1577    2255    3126 
   Size:        874      70     633     678     871

  Fragments arranged by size: 

                 874     871     678     633      70
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     520     631     957    1453    1669    3123    3126 
   Size:        520     111     326     496     216    1454       3

  Fragments arranged by size: 

                1454     520     496     326     216     111       3
 
TatI w'GTAC_w

Cuts at:      0    1013    1049    2321    3126 
   Size:       1013      36    1272     805

  Fragments arranged by size: 

                1272    1013     805      36
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     804     836    2767    2799    2929    2961    3065    3097 
   Size:        804      32    1931      32     130      32     104      32

Cuts at:   3097    3126 
   Size:         29

  Fragments arranged by size: 

                1931     804     130     104      32      32      32      32
                  29
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1419    1641    3117    3126 
   Size:       1419     222    1476       9

  Fragments arranged by size: 

                1476    1419     222       9
 
XmaI C'CCGG_G

Cuts at:      0    1765    2170    3126 
   Size:       1765     405     956

  Fragments arranged by size: 

                1765     956     405
 
XmnI GAAnn'nnTTC

Cuts at:      0     691    3126 
   Size:        691    2435
 

 Enzymes that do cut:

   Acc65I     AcuI     AfeI   AflIII     AleI     AlfI    AlwNI     ApaI
     AscI     AvaI     BanI    BanII     BbeI    BbvCI     BcgI     BglI
     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspEI    BspMI    BsrBI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI   BstEII    BstXI    BstYI   Bsu36I
     BtgI    BtgZI     BtsI    CspCI   DraIII     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I     FseI     FspI
    HaeII    Hin4I     KasI     KpnI     MfeI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI   NgoMIV     NruI     PasI    PflMI
     PfoI     PmlI     PpiI    PpuMI   PspOMI     PstI    PvuII     SacI
    SacII    SanDI     SapI     SfcI     SfoI     SmaI     SmlI     StuI
     StyI    TaqII     TatI     TstI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI    AflII     AgeI     AhdI     AloI
    ApaLI     ApoI     AseI    AsiSI    AvrII     BaeI    BamHI     BbsI
    BciVI     BclI    BfrBI    BglII     BmtI    BsaBI    BsiWI     BsmI
    BspHI    BsrDI    BstBI  BstZ17I     ClaI     DraI    EcoRI    EcoRV
     FalI    FspAI   HincII  HindIII     HpaI     MluI     NdeI     NheI
     NotI     NsiI     NspI     PacI     PciI     PmeI    PshAI     PsiI
    PspXI     PsrI     PvuI    RsrII     SalI     SbfI     ScaI    SexAI
     SfiI     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI
     SwaI  Tth111I     XbaI     XhoI     ZraI
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