Restriction map for pF1KB4782
##################################
# NO CUT ENZYMES (  111 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AflII       AgeI        AocI        
Aor51HI     ApaI        ApoI        AscI        AseI        
AsnI        Asp718I     AviII       AvrII       BanI        
BbeI        BbrPI       BbvCI       BcgI        BfrI        
BglI        BlnI        BlpI        Bpu1102I    BseAI       
BsiEI       BsiWI       BsmBI       BspDI       BspEI       
BspHI       BsrBI       BsrDI       BsrFI       BssHII      
Bst1107I    BstBI       BstUI       BstXI       BstZ17I     
Bsu36I      CelII       Cfr10I      ClaI        CpoI        
DraI        DrdI        EagI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoRI       EcoRV       
FauI        FseI        FspI        HincII      HindII      
HindIII     HpaI        KasI        KpnI        KspI        
MfeI        MluI        MroI        MunI        MvnI        
NaeI        NarI        NdeI        NgoMIV      NheI        
NotI        NruI        NspV        PacI        PinAI       
PmaCI       PmeI        PmlI        PshBI       Psp1406I    
PstI        PvuI        RcaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        SfcI        
SfiI        SfoI        SfuI        SgrAI       SnaBI       
SpeI        Sse8387I    SspI        SwaI        Tsp509I     
XbaI        


###################################
# ONE CUT ENZYMES (   70 enzymes) #
###################################

AhdI          787  GACATCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I        997  GTGCAC               G^TGCAC
AlwNI          52  CAGAGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaLI         997  GTGCAC               G^TGCAC
Asp700I       858  GAATCTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI         787  GACATCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI          367  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI         1007  TGGCCA               TGG^CCA
BamHI         755  GGATCC               G^GATCC
BanII         834  GAGCCC               G[GA]GC[CT]^C
BciVI         991  GGATAC               GTATCC(6/5)
BclI          500  TGATCA               T^GATCA
BfaI          721  CTAG                 C^TAG
BglII         332  AGATCT               A^GATCT
BsaAI         994  TACGTG               [CT]AC^GT[GA]
BsaBI        1017  GATGCACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI         747  ACCGGA               [AT]^CCGG[AT]
BseRI          46  GAGGAG               GAGGAG(10/8)
BsmI          591  GCATTC               GAATGC(1/-1)
Bsp1407I      278  TGTACA               T^GTACA
BspLU11I      740  ACATGT               A^CATGT
BspMI         289  ACCTGC               ACCTGC(4/8)
BsrGI         278  TGTACA               T^GTACA
BsrI          942  ACTGG                ACTGG(1/-1)
BssSI          82  CTCGTG               CACGAG(-5/-1)
BstAPI         51  GCAGAGCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        667  GGTGACC              G^GT[ACGT]ACC
BstPI         667  GGTGACC              G^GT[ACGT]ACC
CfoI          575  GCGC                 GCG^C
DdeI          698  CTGAG                C^T[ACGT]AG
DraIII       1000  CACCAGGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I      787  GACATCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI          862  CTCTTC               CTCTTC(1/4)
EcoO65I       667  GGTGACC              G^GT[ACGT]ACC
EcoT22I       589  ATGCAT               ATGCA^T
FbaI          500  TGATCA               T^GATCA
HaeII         574  AGCGCC               [GA]GCGC^[CT]
HhaI          575  GCGC                 GCG^C
HinP1I        575  GCGC                 G^CGC
Ksp632I       862  CTCTTC               CTCTTC(1/4)
MaeI          721  CTAG                 C^TAG
MamI         1017  GATGCACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI        1007  TGGCCA               TGG^CCA
MscI         1007  TGGCCA               TGG^CCA
MseI          512  TTAA                 T^TAA
MslI         1023  CATCTGGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        565  CAGCTG               C[AC]G^C[GT]G
NcoI          129  CCATGG               C^CATGG
NsiI          589  ATGCAT               ATGCA^T
PaeR7I         68  CTCGAG               C^TCGAG
PflFI         367  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         156  CCACACCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          430  GACTC                GAGTC(4/5)
PpuMI         485  GGGACCT              [GA]G^G[AT]CC[CT]
PshAI         670  GACCTTGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII         565  CAGCTG               CAG^CTG
SexAI        1001  ACCAGGT              A^CC[AT]GGT
SmaI          910  CCCGGG               CCC^GGG
SmlI           68  CTCGAG               C^T[CT][GA]AG
SphI           94  GCATGC               GCATG^C
SspBI         278  TGTACA               T^GTACA
StuI           64  AGGCCT               AGG^CCT
TfiI          858  GAATC                G^A[AT]TC
Tru9I         512  TTAA                 T^TAA
Tth111I       367  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        156  CCACACCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI           68  CTCGAG               C^TCGAG
XmaCI         910  CCCGGG               C^CCGGG
XmaI          910  CCCGGG               C^CCGGG
XmnI          858  GAATCTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AflIII        632  ACGTGT               A^C[GA][CT]GT
              740  ACATGT              
AlwI          756  GATCC                GGATC(4/5)
              755  GGATC               
AvaI           68  CTCGAG               C^[CT]CG[GA]G
              910  CCCGGG              
AvaII         486  GGACC                G^G[AT]CC
              794  GGTCC               
BbsI          306  GAAGAC               GAAGAC(2/6)
              442  GAAGAC              
BpmI          829  CTCCAG               CTGGAG(16/14)
              466  CTGGAG              
BpuAI         306  GAAGAC               GAAGAC(2/6)
              442  GAAGAC              
BsaI          700  GAGACC               GGTCTC(1/5)
              978  GAGACC              
BsmFI         485  GGGAC                GGGAC(10/14)
              727  GGGAC               
BsoBI          68  CTCGAG               C^[CT]CG[GA]G
              910  CCCGGG              
BstYI         332  AGATCT               [GA]^GATC[CT]
              755  GGATCC              
DraII         165  GGGGCCT              [GA]G^G[ACGT]CC[CT]
              485  GGGACCT             
EaeI          117  CGGCCA               [CT]^GGCC[GA]
             1007  TGGCCA              
EcoO109I      165  GGGGCCT              [GA]G^G[ACGT]CC[CT]
              485  GGGACCT             
HinfI         430  GACTC                G^A[ACGT]TC
              858  GAATC               
MaeII         632  ACGT                 A^CGT
              995  ACGT                
MflI          332  AGATCT               [GA]^GATC[CT]
              755  GGATCC              
ScaI          506  AGTACT               AGT^ACT
              554  AGTACT              
TspRI         311  CAGTG                CA[GC]TG[ACGT][ACGT]^
              889  CAGTG               
XcmI          120  CCACATGCCCCATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              287  CCACCTGCCTGCTGG     
XhoII         332  AGATCT               [GA]^GATC[CT]
              755  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   56 enzymes) #
#########################################

AciI        AcyI        AfaI        AluI        AspHI       
BbvI        BcnI        BmyI        BsaHI       BsaJI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
Bsp1286I    BssKI       BstF5I      BstNI       Cac8I       
Cfr13I      DpnII       DsaI        EcoRII      EcoT14I     
Fnu4HI      FokI        HaeIII      HapII       HgaI        
Hin1I       HpaII       HphI        ItaI        MaeIII      
MboI        MboII       MnlI        MspI        MvaI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
NspI        RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       StyI        TaqI        TseI        Tsp45I      
TthHB8I     
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