Restriction map for pF1KB4745
 (Linear) (Six-base) MAPSORT of: KIBB4745.seq  Check: 2688  from: 1  to: 1119



KIBB4745.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:26  ..

AccI GT'mk_AC

Cuts at:      0     839    1119 
   Size:        839     280
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     280    1119 
   Size:        280     839
 
AfeI AGC'GCT

Cuts at:      0     421    1119 
   Size:        421     698
 
AleI CACnn'nnGTG

Cuts at:      0     697    1119 
   Size:        697     422
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     163     195    1119 
   Size:        163      32     924

  Fragments arranged by size: 

                 924     163      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0      96     609    1119 
   Size:         96     513     510

  Fragments arranged by size: 

                 513     510      96
 
ApaI G_GGCC'C

Cuts at:      0     341    1119 
   Size:        341     778
 
ApaLI G'TGCA_C

Cuts at:      0     971    1119 
   Size:        971     148
 
AvaI C'yCGr_G

Cuts at:      0     345    1119 
   Size:        345     774
 
BanII G_rGCy'C

Cuts at:      0      33     341     551    1119 
   Size:         33     308     210     568

  Fragments arranged by size: 

                 568     308     210      33
 
Bme1580I G_kGCm'C

Cuts at:      0     341     975    1119 
   Size:        341     634     144

  Fragments arranged by size: 

                 634     341     144
 
BmgBI CAC'GTC

Cuts at:      0     864    1119 
   Size:        864     255
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     344    1119 
   Size:        344     775
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     479     655     799     937    1119 
   Size:        479     176     144     138     182

  Fragments arranged by size: 

                 479     182     176     144     138
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      18    1119 
   Size:         18    1101
 
BsaAI yAC'GTr

Cuts at:      0      39    1119 
   Size:         39    1080
 
BsaHI Gr'CG_yC

Cuts at:      0     871    1119 
   Size:        871     248
 
BsaWI w'CCGG_w

Cuts at:      0     590    1119 
   Size:        590     529
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     163     193    1119 
   Size:        163      30     926

  Fragments arranged by size: 

                 926     163      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     461     577     652    1119 
   Size:        461     116      75     467

  Fragments arranged by size: 

                 467     461     116      75
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      19     622     697     708    1119 
   Size:         19     603      75      11     411

  Fragments arranged by size: 

                 603     411      75      19      11
 
BsiHKAI G_wGCw'C

Cuts at:      0      33     551     795     812     975    1119 
   Size:         33     518     244      17     163     144

  Fragments arranged by size: 

                 518     244     163     144      33      17
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1086    1119 
   Size:       1086      33
 
Bsp1286I G_dGCh'C

Cuts at:      0      33     341     551     795     812     975    1119 
   Size:         33     308     210     244      17     163     144

  Fragments arranged by size: 

                 308     244     210     163     144      33      17
 
BspEI T'CCGG_A

Cuts at:      0     590    1119 
   Size:        590     529
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     402    1119 
   Size:        402     717
 
BsrBI CCG'CTC

Cuts at:      0     621    1119 
   Size:        621     498
 
BsrFI r'CCGG_y

Cuts at:      0     727     892    1040    1119 
   Size:        727     165     148      79

  Fragments arranged by size: 

                 727     165     148      79
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     609    1119 
   Size:        609     510
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     711    1024    1119 
   Size:        711     313      95

  Fragments arranged by size: 

                 711     313      95
 
BstYI r'GATC_y

Cuts at:      0     581    1119 
   Size:        581     538
 
BtgI C'CryG_G

Cuts at:      0     167     323     914     992    1119 
   Size:        167     156     591      78     127

  Fragments arranged by size: 

                 591     167     156     127      78
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     894    1119 
   Size:        894     225
 
BtsI GCAGTG_nn'

Cuts at:      0     792    1119 
   Size:        792     327
 
DraIII CAC_nnn'GTG

Cuts at:      0      42     147    1119 
   Size:         42     105     972

  Fragments arranged by size: 

                 972     105      42
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1078    1119 
   Size:       1078      41
 
EaeI y'GGCC_r

Cuts at:      0     320     701     917    1119 
   Size:        320     381     216     202

  Fragments arranged by size: 

                 381     320     216     202
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     738    1119 
   Size:        738     381
 
EcoICRI GAG'CTC

Cuts at:      0      31     549    1119 
   Size:         31     518     570

  Fragments arranged by size: 

                 570     518      31
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     280     479     655     799     937    1119 
   Size:        280     199     176     144     138     182

  Fragments arranged by size: 

                 280     199     182     176     144     138
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     592    1119 
   Size:        592     527
 
EcoO109I rG'GnC_Cy

Cuts at:      0       5      93     294     338    1071    1119 
   Size:          5      88     201      44     733      48

  Fragments arranged by size: 

                 733     201      88      48      44       5
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     150     243    1119 
   Size:        150      93     876

  Fragments arranged by size: 

                 876     150      93
 
HaeII r_GCGC'y

Cuts at:      0     423    1119 
   Size:        423     696
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     653     685     870     902    1119 
   Size:        653      32     185      32     217

  Fragments arranged by size: 

                 653     217     185      32      32
 
HincII GTy'rAC

Cuts at:      0     930    1119 
   Size:        930     189
 
MscI TGG'CCA

Cuts at:      0     322     703     919    1119 
   Size:        322     381     216     200

  Fragments arranged by size: 

                 381     322     216     200
 
MslI CAynn'nnrTG

Cuts at:      0     115     143     436     673     697     913    1119 
   Size:        115      28     293     237      24     216     206

  Fragments arranged by size: 

                 293     237     216     206     115      28      24
 
MspA1I CmG'CkG

Cuts at:      0      87     606     945    1119 
   Size:         87     519     339     174

  Fragments arranged by size: 

                 519     339     174      87
 
NaeI GCC'GGC

Cuts at:      0     894    1042    1119 
   Size:        894     148      77

  Fragments arranged by size: 

                 894     148      77
 
NcoI C'CATG_G

Cuts at:      0     992    1119 
   Size:        992     127
 
NgoMIV G'CCGG_C

Cuts at:      0     892    1040    1119 
   Size:        892     148      79

  Fragments arranged by size: 

                 892     148      79
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     147    1119 
   Size:        147     972
 
PmlI CAC'GTG

Cuts at:      0      39    1119 
   Size:         39    1080
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     163     195    1119 
   Size:        163      32     924

  Fragments arranged by size: 

                 924     163      32
 
PpuMI rG'GwC_Cy

Cuts at:      0       5    1071    1119 
   Size:          5    1066      48

  Fragments arranged by size: 

                1066      48       5
 
PspOMI G'GGCC_C

Cuts at:      0     337    1119 
   Size:        337     782
 
PstI C_TGCA'G

Cuts at:      0      92     284    1119 
   Size:         92     192     835

  Fragments arranged by size: 

                 835     192      92
 
PvuII CAG'CTG

Cuts at:      0      87     606    1119 
   Size:         87     519     513

  Fragments arranged by size: 

                 519     513      87
 
SacI G_AGCT'C

Cuts at:      0      33     551    1119 
   Size:         33     518     568

  Fragments arranged by size: 

                 568     518      33
 
SanDI GG'GwC_CC

Cuts at:      0       5    1119 
   Size:          5    1114
 
SexAI A'CCwGG_T

Cuts at:      0     710    1119 
   Size:        710     409
 
SfcI C'TryA_G

Cuts at:      0      88     280    1119 
   Size:         88     192     839

  Fragments arranged by size: 

                 839     192      88
 
SmaI CCC'GGG

Cuts at:      0     347    1119 
   Size:        347     772
 
StuI AGG'CCT

Cuts at:      0     391     958    1119 
   Size:        391     567     161

  Fragments arranged by size: 

                 567     391     161
 
StyI C'CwwG_G

Cuts at:      0     992    1119 
   Size:        992     127
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      40    1119 
   Size:         40    1079
 
TatI w'GTAC_w

Cuts at:      0     665    1119 
   Size:        665     454
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     965     997    1119 
   Size:        965      32     122

  Fragments arranged by size: 

                 965     122      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     912    1119 
   Size:        912     207
 
XmaI C'CCGG_G

Cuts at:      0     345    1119 
   Size:        345     774
 

 Enzymes that do cut:

     AccI     AcuI     AfeI     AleI     AloI    AlwNI     ApaI    ApaLI
     AvaI    BanII Bme1580I    BmgBI     BmrI     BpmI     BsaI    BsaAI
    BsaHI    BsaWI    BsaXI    BseRI     BsgI  BsiHKAI    BsmBI Bsp1286I
    BspEI    BspMI    BsrBI    BsrFI   BstAPI    BstXI    BstYI     BtgI
    BtgZI     BtsI   DraIII     DrdI     EaeI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I     FalI    HaeII    Hin4I   HincII     MscI     MslI
   MspA1I     NaeI     NcoI   NgoMIV    PflMI     PmlI     PpiI    PpuMI
   PspOMI     PstI    PvuII     SacI    SanDI    SexAI     SfcI     SmaI
     StuI     StyI    TaqII     TatI     TstI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI    AflII   AflIII     AgeI     AhdI
     AlfI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI
     BanI     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI     BmtI     BplI   Bpu10I    BpuEI    BsaBI
    BseYI    BsiEI    BsiWI     BsmI    BspHI    BsrDI    BsrGI   BssHII
    BssSI    BstBI   BstEII  BstZ17I   Bsu36I     ClaI    CspCI     DraI
     EagI     EarI    EcoRI    EcoRV     FseI     FspI    FspAI  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     NarI     NdeI
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
     PfoI     PmeI    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII
    SacII     SalI     SapI     SbfI     ScaI     SfiI     SfoI     SgfI
    SgrAI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI
  Tth111I     XbaI     XhoI     XmnI     ZraI
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