Restriction map for pF1KB4712
##################################
# NO CUT ENZYMES (  151 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AcsI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AlwNI       AocI        ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AvaI        AviII       AvrII       
BamHI       BanI        BanII       BbeI        BbrPI       
BbvCI       BcgI        BciVI       BfaI        BfrI        
BglII       BlnI        BsaAI       BsaBI       BseAI       
BsiEI       BsiWI       BsmBI       BsmI        BsoBI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrFI       BsrGI       BssHII      Bst1107I    
BstBI       BstEII      BstPI       BstXI       BstZ17I     
Bsu36I      Cfr10I      ClaI        CpoI        DraI        
DraII       DraIII      DrdI        EagI        Eam1105I    
EclXI       Eco52I      Eco81I      EcoO109I    EcoO65I     
EcoRI       EcoRV       EcoT14I     EcoT22I     FauI        
FseI        FspI        HincII      HindII      HindIII     
HpaI        HphI        KasI        KpnI        MaeI        
MamI        MfeI        MluI        MroI        MslI        
MunI        NaeI        NarI        NcoI        NdeI        
NgoMIV      NheI        NlaIV       NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       PvuI        PvuII       
RcaI        RsrII       SacI        SalI        SbfI        
ScaI        SexAI       SfaNI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StyI        SwaI        TfiI        Tth111I     Van91I      
XbaI        XcmI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

AclI          151  AACGTT               AA^CGTT
AcyI          607  GACGCC               G[GA]^CG[CT]C
AlwI          555  GATCC                GGATC(4/5)
Aor51HI        53  AGCGCT               AGC^GCT
AspHI         487  GAGCAC               G[AT]GC[AT]^C
BclI          323  TGATCA               T^GATCA
BlpI          300  GCTGAGC              GC^T[ACGT]AGC
BpmI          177  CTGGAG               CTGGAG(16/14)
Bpu1102I      300  GCTGAGC              GC^T[ACGT]AGC
BsaHI         607  GACGCC               G[GA]^CG[CT]C
BsaI          340  GAGACC               GGTCTC(1/5)
BsaJI          71  CCGCGG               C^C[ACGT][ACGT]GG
BsaWI         415  ACCGGA               [AT]^CCGG[AT]
BseRI         673  CTCCTC               GAGGAG(10/8)
BsgI           22  GTGCAG               GTGCAG(16/14)
BsiHKAI       487  GAGCAC               G[AT]GC[AT]^C
BsmAI         340  GAGAC                GTCTC(1/5)
BsmFI         383  GGGAC                GGGAC(10/14)
BsrBI         168  CCGCTC               CCGCTC(-3/-3)
BsrDI         221  GCAATG               GCAATG(2/0)
BssSI         472  CACGAG               CACGAG(-5/-1)
BstAPI         15  GCAACTGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI         554  AGATCC               [GA]^GATC[CT]
CelII         300  GCTGAGC              GC^T[ACGT]AGC
CfoI           54  GCGC                 GCG^C
DdeI          301  CTGAG                C^T[ACGT]AG
DsaI           71  CCGCGG               C^C[GA][CT]GG
EarI          171  CTCTTC               CTCTTC(1/4)
Eco47III       53  AGCGCT               AGC^GCT
EcoNI         630  CCTCAACGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI          323  TGATCA               T^GATCA
HaeII          53  AGCGCT               [GA]GCGC^[CT]
HgaI          607  GACGC                GACGC(5/10)
HhaI           54  GCGC                 GCG^C
Hin1I         607  GACGCC               G[GA]^CG[CT]C
HinP1I         54  GCGC                 G^CGC
Ksp632I       171  CTCTTC               CTCTTC(1/4)
KspI           71  CCGCGG               CCGC^GG
MaeIII         88  GTGAC                ^GT[ACGT]AC
MflI          554  AGATCC               [GA]^GATC[CT]
MseI          530  TTAA                 T^TAA
MspA1I         71  CCGCGG               C[AC]G^C[GT]G
NspI          491  ACATGC               [GA]CATG^[CT]
Psp1406I      151  AACGTT               AA^CGTT
PstI          212  CTGCAG               CTGCA^G
SacII          71  CCGCGG               CCGC^GG
SapI          170  GCTCTTC              GCTCTTC(1/4)
Tru9I         530  TTAA                 T^TAA
Tsp45I         88  GTGAC                ^GT[GC]AC
Tsp509I       331  AATT                 ^AATT
TspRI         107  CACTG                CA[GC]TG[ACGT][ACGT]^
XhoII         554  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AfaI          252  GTAC                 GT^AC
              348  GTAC                
AvaII           6  GGACC                G^G[AT]CC
              243  GGTCC               
BalI          410  TGGCCA               TGG^CCA
              587  TGGCCA              
BbsI          204  GAAGAC               GAAGAC(2/6)
              541  GTCTTC              
BcnI           32  CCCGG                CC^[GC]GG
              255  CCGGG               
BglI           31  GCCCGGCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
               40  GCCGAGCAGGC         
BmyI          161  GGGCAC               G[AGT]GC[ACT]^C
              487  GAGCAC              
BpuAI         204  GAAGAC               GAAGAC(2/6)
              541  GTCTTC              
BsiYI         393  CCGCTACCTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              630  CCTCAACGAGG         
BslI          393  CCGCTACCTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              630  CCTCAACGAGG         
Bsp1286I      161  GGGCAC               G[AGT]GC[ACT]^C
              487  GAGCAC              
BsrI           18  ACTGG                ACTGG(1/-1)
              345  CCAGT               
BstF5I        510  CATCC                GGATG(2/0)
              294  GGATG               
Cfr13I          6  GGACC                G^G[ACGT]CC
              243  GGTCC               
FokI          510  CATCC                GGATG(9/13)
              294  GGATG               
MaeII          86  ACGT                 A^CGT
              152  ACGT                
MluNI         410  TGGCCA               TGG^CCA
              587  TGGCCA              
MscI          410  TGGCCA               TGG^CCA
              587  TGGCCA              
NciI           32  CCCGG                CC^[GC]GG
              255  CCGGG               
RsaI          252  GTAC                 GT^AC
              348  GTAC                
Sau96I          6  GGACC                G^G[ACGT]CC
              243  GGTCC               
SfcI          212  CTGCAG               C^T[GA][CT]AG
              459  CTACAG              
StuI          455  AGGCCT               AGG^CCT
              519  AGGCCT              
TaqI          112  TCGA                 T^CGA
              602  TCGA                
TthHB8I       112  TCGA                 T^CGA
              602  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   30 enzymes) #
#########################################

AccII       AciI        AluI        BbvI        BssKI       
BstNI       BstUI       Cac8I       DpnII       EaeI        
EcoRII      Fnu4HI      HaeIII      HapII       HinfI       
HpaII       ItaI        MboI        MboII       MnlI        
MspI        MvaI        MvnI        MwoI        NdeII       
NlaIII      PleI        Sau3AI      ScrFI       TseI        
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