Restriction map for pF1KB4688
##################################
# NO CUT ENZYMES (  134 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AviII       AvrII       BbeI        
BbrPI       BbvCI       BciVI       BfrI        BglII       
BlnI        BlpI        Bpu1102I    BsaBI       BsaHI       
BsaWI       BseAI       BsiEI       BsiHKAI     BsiWI       
BsmBI       BsmI        Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrBI       BsrFI       BsrGI       
BssHII      Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstXI       BstZ17I     Bsu36I      CelII       
Cfr10I      ClaI        CpoI        DraII       DraIII      
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRV       EcoT22I     FseI        FspI        HaeII       
HgaI        Hin1I       HindIII     HpaI        KasI        
KpnI        KspI        MamI        MluI        MroI        
MslI        NaeI        NarI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SnaBI       
SphI        Sse8387I    SspBI       SspI        SwaI        
Tth111I     XbaI        XhoI        XmnI        


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# ONE CUT ENZYMES (   37 enzymes) #
###################################

AccI          143  GTAGAC               GT^[AC][GT]AC
AccII         941  CGCG                 CG^CG
AlwNI        1231  CAGCAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
BamHI         473  GGATCC               G^GATCC
BanII         487  GAGCCC               G[GA]GC[CT]^C
BbsI         1110  GAAGAC               GAAGAC(2/6)
BcgI          450  CGATGCTGCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI         1586  TGATCA               T^GATCA
BglI          495  GCCAGAGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI        1110  GAAGAC               GAAGAC(2/6)
BsaAI        1438  CACGTA               [CT]AC^GT[GA]
BsgI          194  GTGCAG               GTGCAG(16/14)
BsrDI        1025  GCAATG               GCAATG(2/0)
BssSI        1458  CACGAG               CACGAG(-5/-1)
BstUI         941  CGCG                 CG^CG
DraI         1413  TTTAAA               TTT^AAA
EcoRI        1247  GAATTC               G^AATTC
FbaI         1586  TGATCA               T^GATCA
HapII         615  CCGG                 C^CGG
HincII        277  GTTGAC               GT[CT]^[GA]AC
HindII        277  GTTGAC               GT[CT]^[GA]AC
HpaII         615  CCGG                 C^CGG
MaeII        1439  ACGT                 A^CGT
MfeI         1563  CAATTG               C^AATTG
MspI          615  CCGG                 C^CGG
MunI         1563  CAATTG               C^AATTG
MvnI          941  CGCG                 CG^CG
NspI         1040  ACATGC               [GA]CATG^[CT]
PshAI        1706  GACCAATGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SmaI          614  CCCGGG               CCC^GGG
SpeI         1186  ACTAGT               A^CTAGT
StuI          933  AGGCCT               AGG^CCT
TaqI          605  TCGA                 T^CGA
Tsp45I        649  GTGAC                ^GT[GC]AC
TthHB8I       605  TCGA                 T^CGA
XmaCI         614  CCCGGG               C^CCGGG
XmaI          614  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

BalI          843  TGGCCA               TGG^CCA
             1016  TGGCCA              
BcnI          614  CCCGG                CC^[GC]GG
              615  CCGGG               
BpmI          505  CTGGAG               CTGGAG(16/14)
             1045  CTGGAG              
BsaI         1405  GAGACC               GGTCTC(1/5)
             1461  GAGACC              
BsmFI        1183  GGGAC                GGGAC(10/14)
             1066  GTCCC               
BspMI         139  GCAGGT               ACCTGC(4/8)
              963  GCAGGT              
EaeI          843  TGGCCA               [CT]^GGCC[GA]
             1016  TGGCCA              
MluNI         843  TGGCCA               TGG^CCA
             1016  TGGCCA              
MscI          843  TGGCCA               TGG^CCA
             1016  TGGCCA              
MspA1I        197  CAGCTG               C[AC]G^C[GT]G
             1234  CAGCTG              
NciI          614  CCCGG                CC^[GC]GG
              615  CCGGG               
PleI         1493  GAGTC                GAGTC(4/5)
             1615  GAGTC               
PvuII         197  CAGCTG               CAG^CTG
             1234  CAGCTG              
XcmI         1004  CCAACAAGAAGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1437  CCACGTACCCCATGG     


#########################################
# 3 OR MORE CUT ENZYMES (   73 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        AvaI        AvaII       BanI        BbvI        
BfaI        BmyI        BsaJI       BseRI       BsiYI       
BslI        BsmAI       BsoBI       Bsp1286I    BsrI        
BssKI       BstF5I      BstNI       BstYI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DsaI        
EarI        EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeIII      HhaI        HinP1I      HinfI       
HphI        ItaI        Ksp632I     MaeI        MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MvaI        MwoI        NcoI        NdeII       NlaIII      
NlaIV       PflMI       PstI        RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
StyI        TfiI        Tru9I       TseI        Tsp509I     
TspRI       Van91I      XhoII       
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