Restriction map for pF1KB4641
 (Linear) (Six-base) MAPSORT of: KIBB4641.seq  Check: 863  from: 1  to: 2907



KIBB4641.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:26  ..

AclI AA'CG_TT

Cuts at:      0    1482    2907 
   Size:       1482    1425
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1522    2273    2907 
   Size:       1522     751     634

  Fragments arranged by size: 

                1522     751     634
 
AflIII A'CryG_T

Cuts at:      0    1001    1192    2907 
   Size:       1001     191    1715

  Fragments arranged by size: 

                1715    1001     191
 
AlwNI CAG_nnn'CTG

Cuts at:      0    2003    2907 
   Size:       2003     904
 
ApoI r'AATT_y

Cuts at:      0     550     703     763     956    1336    1734    1965    2065 
   Size:        550     153      60     193     380     398     231     100

Cuts at:   2065    2678    2721    2907 
   Size:        613      43     186

  Fragments arranged by size: 

                 613     550     398     380     231     193     186     153
                 100      60      43
 
AvaI C'yCGr_G

Cuts at:      0      58    2907 
   Size:         58    2849
 
AvrII C'CTAG_G

Cuts at:      0    2766    2907 
   Size:       2766     141
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    2006    2039    2907 
   Size:       2006      33     868

  Fragments arranged by size: 

                2006     868      33
 
BanI G'GyrC_C

Cuts at:      0    1607    2770    2907 
   Size:       1607    1163     137

  Fragments arranged by size: 

                1607    1163     137
 
BanII G_rGCy'C

Cuts at:      0     113    2315    2907 
   Size:        113    2202     592

  Fragments arranged by size: 

                2202     592     113
 
BbeI G_GCGC'C

Cuts at:      0    1611    2907 
   Size:       1611    1296
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1121    2887    2907 
   Size:       1121    1766      20

  Fragments arranged by size: 

                1766    1121      20
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2578    2612    2907 
   Size:       2578      34     295

  Fragments arranged by size: 

                2578     295      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    2007    2907 
   Size:       2007     900
 
BfrBI ATG'CAT

Cuts at:      0    1237    1775    2691    2695    2907 
   Size:       1237     538     916       4     212

  Fragments arranged by size: 

                1237     916     538     212       4
 
BglII A'GATC_T

Cuts at:      0    1164    2907 
   Size:       1164    1743
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    2198    2907 
   Size:       2198     709
 
BmtI G_CTAG'C

Cuts at:      0    1618    2907 
   Size:       1618    1289
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      40     127     427     736    1463    2907 
   Size:         40      87     300     309     727    1444

  Fragments arranged by size: 

                1444     727     309     300      87      40
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     530    1250    2366    2907 
   Size:        530     720    1116     541

  Fragments arranged by size: 

                1116     720     541     530
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      79    2907 
   Size:         79    2828
 
BsaAI yAC'GTr

Cuts at:      0    1002    1071    1195    2907 
   Size:       1002      69     124    1712

  Fragments arranged by size: 

                1712    1002     124      69
 
BsaBI GATnn'nnATC

Cuts at:      0    2522    2907 
   Size:       2522     385
 
BsaHI Gr'CG_yC

Cuts at:      0    1608    2780    2907 
   Size:       1608    1172     127

  Fragments arranged by size: 

                1608    1172     127
 
BsaWI w'CCGG_w

Cuts at:      0    2442    2907 
   Size:       2442     465
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      76     258    1049    1357    1738    2488    2907 
   Size:         76     182     791     308     381     750     419

  Fragments arranged by size: 

                 791     750     419     381     308     182      76
 
BseYI C'CCAG_C

Cuts at:      0      38     384    2907 
   Size:         38     346    2523

  Fragments arranged by size: 

                2523     346      38
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     150    2907 
   Size:        150    2757
 
BsiHKAI G_wGCw'C

Cuts at:      0      80    2121    2907 
   Size:         80    2041     786

  Fragments arranged by size: 

                2041     786      80
 
BsmI GAATG_Cn'

Cuts at:      0     116     837    2335    2907 
   Size:        116     721    1498     572

  Fragments arranged by size: 

                1498     721     572     116
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      40     383    2907 
   Size:         40     343    2524

  Fragments arranged by size: 

                2524     343      40
 
Bsp1286I G_dGCh'C

Cuts at:      0      80     113    2121    2315    2907 
   Size:         80      33    2008     194     592

  Fragments arranged by size: 

                2008     592     194      80      33
 
BspEI T'CCGG_A

Cuts at:      0    2442    2907 
   Size:       2442     465
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1446    2375    2907 
   Size:       1446     929     532

  Fragments arranged by size: 

                1446     929     532
 
BsrDI GCAATG_nn'

Cuts at:      0    1221    2538    2846    2907 
   Size:       1221    1317     308      61

  Fragments arranged by size: 

                1317    1221     308      61
 
BssSI C'ACGA_G

Cuts at:      0    2259    2907 
   Size:       2259     648
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     888    2833    2907 
   Size:        888    1945      74

  Fragments arranged by size: 

                1945     888      74
 
BstEII G'GTnAC_C

Cuts at:      0    1433    2907 
   Size:       1433    1474
 
BstYI r'GATC_y

Cuts at:      0    1077    1164    2511    2907 
   Size:       1077      87    1347     396

  Fragments arranged by size: 

                1347    1077     396      87
 
BtgI C'CryG_G

Cuts at:      0       7    2133    2907 
   Size:          7    2126     774

  Fragments arranged by size: 

                2126     774       7
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1047    1583    2907 
   Size:       1047     536    1324

  Fragments arranged by size: 

                1324    1047     536
 
ClaI AT'CG_AT

Cuts at:      0     224    2907 
   Size:        224    2683
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     657     692    2907 
   Size:        657      35    2215

  Fragments arranged by size: 

                2215     657      35
 
DraI TTT'AAA

Cuts at:      0    2883    2907 
   Size:       2883      24
 
DraIII CAC_nnn'GTG

Cuts at:      0    1765    2907 
   Size:       1765    1142
 
EaeI y'GGCC_r

Cuts at:      0    1053    2005    2907 
   Size:       1053     952     902

  Fragments arranged by size: 

                1053     952     902
 
EarI CTCTTCn'nnn_

Cuts at:      0    1403    1715    2048    2907 
   Size:       1403     312     333     859

  Fragments arranged by size: 

                1403     859     333     312
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      58    2076    2907 
   Size:         58    2018     831

  Fragments arranged by size: 

                2018     831      58
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      40     127     427     736    1463    1522    2273    2907 
   Size:         40      87     300     309     727      59     751     634

  Fragments arranged by size: 

                 751     727     634     309     300      87      59      40
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1462    2907 
   Size:       1462    1445
 
EcoO109I rG'GnC_Cy

Cuts at:      0     995    2139    2364    2907 
   Size:        995    1144     225     543

  Fragments arranged by size: 

                1144     995     543     225
 
EcoRV GAT'ATC

Cuts at:      0    2558    2907 
   Size:       2558     349
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1611    2907 
   Size:       1611    1296
 
FspI TGC'GCA

Cuts at:      0     616    2907 
   Size:        616    2291
 
HaeII r_GCGC'y

Cuts at:      0      39    1611    2907 
   Size:         39    1572    1296

  Fragments arranged by size: 

                1572    1296      39
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      52      84     380     412     574     606     742     774 
   Size:         52      32     296      32     162      32     136      32

Cuts at:    774    1069    1101    1979    2011    2547    2579    2907 
   Size:        295      32     878      32     536      32     328

  Fragments arranged by size: 

                 878     536     328     296     295     162     136      52
                  32      32      32      32      32      32      32
 
HincII GTy'rAC

Cuts at:      0     300     746    2907 
   Size:        300     446    2161

  Fragments arranged by size: 

                2161     446     300
 
HindIII A'AGCT_T

Cuts at:      0     691    2907 
   Size:        691    2216
 
HpaI GTT'AAC

Cuts at:      0     746    2907 
   Size:        746    2161
 
KasI G'GCGC_C

Cuts at:      0    1607    2907 
   Size:       1607    1300
 
MfeI C'AATT_G

Cuts at:      0    2729    2822    2907 
   Size:       2729      93      85

  Fragments arranged by size: 

                2729      93      85
 
MscI TGG'CCA

Cuts at:      0    1055    2007    2907 
   Size:       1055     952     900

  Fragments arranged by size: 

                1055     952     900
 
MslI CAynn'nnrTG

Cuts at:      0     989    2907 
   Size:        989    1918
 
MspA1I CmG'CkG

Cuts at:      0       9      18    1629    2907 
   Size:          9       9    1611    1278

  Fragments arranged by size: 

                1611    1278       9       9
 
NarI GG'CG_CC

Cuts at:      0    1608    2907 
   Size:       1608    1299
 
NdeI CA'TA_TG

Cuts at:      0    2197    2907 
   Size:       2197     710
 
NheI G'CTAG_C

Cuts at:      0    1614    2907 
   Size:       1614    1293
 
NsiI A_TGCA'T

Cuts at:      0    1239    1777    2693    2697    2907 
   Size:       1239     538     916       4     210

  Fragments arranged by size: 

                1239     916     538     210       4
 
NspI r_CATG'y

Cuts at:      0     384    2695    2907 
   Size:        384    2311     212

  Fragments arranged by size: 

                2311     384     212
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1087    2210    2907 
   Size:       1087    1123     697

  Fragments arranged by size: 

                1123    1087     697
 
PfoI T'CCnGG_A

Cuts at:      0    1973    2907 
   Size:       1973     934
 
PmlI CAC'GTG

Cuts at:      0    1071    2907 
   Size:       1071    1836
 
PpuMI rG'GwC_Cy

Cuts at:      0     995    2364    2907 
   Size:        995    1369     543

  Fragments arranged by size: 

                1369     995     543
 
PsiI TTA'TAA

Cuts at:      0    1526    2907 
   Size:       1526    1381
 
PstI C_TGCA'G

Cuts at:      0      17    1829    2907 
   Size:         17    1812    1078

  Fragments arranged by size: 

                1812    1078      17
 
PvuII CAG'CTG

Cuts at:      0      18    1629    2907 
   Size:         18    1611    1278

  Fragments arranged by size: 

                1611    1278      18
 
SacII CC_GC'GG

Cuts at:      0      10    2907 
   Size:         10    2897
 
ScaI AGT'ACT

Cuts at:      0     448    1834    2907 
   Size:        448    1386    1073

  Fragments arranged by size: 

                1386    1073     448
 
SfcI C'TryA_G

Cuts at:      0      13    1825    2907 
   Size:         13    1812    1082

  Fragments arranged by size: 

                1812    1082      13
 
SfoI GGC'GCC

Cuts at:      0    1609    2907 
   Size:       1609    1298
 
SmlI C'TyrA_G

Cuts at:      0     545    1265    2381    2907 
   Size:        545     720    1116     526

  Fragments arranged by size: 

                1116     720     545     526
 
SphI G_CATG'C

Cuts at:      0    2695    2907 
   Size:       2695     212
 
SspI AAT'ATT

Cuts at:      0     621    2907 
   Size:        621    2286
 
StyI C'CwwG_G

Cuts at:      0     279     512     729    2766    2775    2907 
   Size:        279     233     217    2037       9     132

  Fragments arranged by size: 

                2037     279     233     217     132       9
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1569    2907 
   Size:       1569    1338
 
TatI w'GTAC_w

Cuts at:      0     446    1832    2907 
   Size:        446    1386    1075

  Fragments arranged by size: 

                1386    1075     446
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1565    1597    2907 
   Size:       1565      32    1310

  Fragments arranged by size: 

                1565    1310      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1991    2907 
   Size:       1991     916
 
XmnI GAAnn'nnTTC

Cuts at:      0    1748    2907 
   Size:       1748    1159
 

 Enzymes that do cut:

     AclI     AcuI   AflIII    AlwNI     ApoI     AvaI    AvrII     BaeI
     BanI    BanII     BbeI     BbsI     BcgI    BciVI    BfrBI    BglII
     BmrI     BmtI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BseRI    BseYI     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I
    BspEI    BspMI    BsrDI    BssSI   BstAPI   BstEII    BstYI     BtgI
    BtgZI     ClaI    CspCI     DraI   DraIII     EaeI     EarI     EciI
  Eco57MI    EcoNI EcoO109I    EcoRV     FalI     FspI    HaeII    Hin4I
   HincII  HindIII     HpaI     KasI     MfeI     MscI     MslI   MspA1I
     NarI     NdeI     NheI     NsiI     NspI    PflMI     PfoI     PmlI
    PpuMI     PsiI     PstI    PvuII    SacII     ScaI     SfcI     SfoI
     SmlI     SphI     SspI     StyI    TaqII     TatI     TstI  Tth111I
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
    BamHI    BbvCI     BclI     BglI     BlpI Bme1580I    BmgBI     BplI
   Bpu10I    BsaXI    BsiEI    BsiWI    BspHI    BsrBI    BsrFI    BsrGI
   BssHII    BstBI    BstXI  BstZ17I   Bsu36I     BtsI     DrdI     EagI
  EcoICRI    EcoRI     FseI    FspAI     KpnI     MluI     MmeI     NaeI
     NcoI   NgoMIV     NotI     NruI     PacI     PasI     PciI     PmeI
     PpiI    PshAI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI
     SalI    SanDI     SapI     SbfI    SexAI     SfiI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SrfI     StuI     SwaI     XbaI     XcmI
     XhoI     XmaI     ZraI
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