Restriction map for pF1KB4590
##################################
# NO CUT ENZYMES (  131 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AgeI        AhdI        Alw44I      AocI        
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        Asp718I     AspEI       AspI        AvaI        
AviII       AvrII       BbeI        BbrPI       BcgI        
BciVI       BglI        BglII       BlnI        BlpI        
Bpu1102I    BsaAI       BsaHI       BsaWI       BseAI       
BsiEI       BsiWI       BsmBI       BsmI        BsoBI       
Bsp1407I    BspDI       BspEI       BspMI       BsrBI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstF5I      BstPI       BstUI       
BstXI       BstZ17I     Bsu36I      CelII       ClaI        
CpoI        DraI        DraII       DraIII      EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO109I    EcoO65I     EcoT22I     FokI        
FseI        FspI        HgaI        Hin1I       HindIII     
HpaI        KasI        KpnI        KspI        MaeII       
MluI        MroI        MvnI        NaeI        NarI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshBI       Psp1406I    PvuI        RsrII       SacII       
SalI        SapI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        SspBI       SspI        StuI        
SwaI        Tth111I     XcmI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AccI          173  GTCTAC               GT^[AC][GT]AC
AflII         244  CTTAAG               C^TTAAG
AflIII       1958  ACATGT               A^C[GA][CT]GT
AlwNI        1912  CAGGCACTG            CAG[ACGT][ACGT][ACGT]^CTG
Asp700I       346  GAACCCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI        1658  GAGCTC               G[AT]GC[AT]^C
BalI          275  TGGCCA               TGG^CCA
BamHI        1009  GGATCC               G^GATCC
BanI          679  GGCACC               G^G[CT][GA]CC
BanII        1658  GAGCTC               G[GA]GC[CT]^C
BbvCI        2002  GCTGAGG              CCTCAGC(-5/-2)
BcnI         1434  CCGGG                CC^[GC]GG
BfrI          244  CTTAAG               C^TTAAG
BmyI         1658  GAGCTC               G[AGT]GC[ACT]^C
BsaBI        1897  GATATTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI      1658  GAGCTC               G[AT]GC[AT]^C
Bsp1286I     1658  GAGCTC               G[AGT]GC[ACT]^C
BspHI        1905  TCATGA               T^CATGA
BspLU11I     1958  ACATGT               A^CATGT
BsrFI        1380  ACCGGC               [GA]^CCGG[CT]
Cfr10I       1380  ACCGGC               [GA]^CCGG[CT]
EcoRI        1078  GAATTC               G^AATTC
EcoRV         919  GATATC               GAT^ATC
FauI         1574  CCCGC                CCCGC(4/6)
HaeII        1705  GGCGCT               [GA]GCGC^[CT]
HincII       1216  GTTGAC               GT[CT]^[GA]AC
HindII       1216  GTTGAC               GT[CT]^[GA]AC
MamI         1897  GATATTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI          907  CAATTG               C^AATTG
MluNI         275  TGGCCA               TGG^CCA
MscI          275  TGGCCA               TGG^CCA
MunI          907  CAATTG               C^AATTG
NciI         1434  CCGGG                CC^[GC]GG
PflMI        1911  CCAGGCACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI        1678  GACCAAAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI         1905  TCATGA               T^CATGA
SacI         1658  GAGCTC               GAGCT^C
SbfI         1020  CCTGCAGG             CCTGCA^GG
SmlI          244  CTTAAG               C^T[CT][GA]AG
Sse8387I     1020  CCTGCAGG             CCTGCA^GG
TaqI         1067  TCGA                 T^CGA
TthHB8I      1067  TCGA                 T^CGA
Van91I       1911  CCAGGCACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI         1650  TCTAGA               T^CTAGA
XmnI          346  GAACCCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

BclI         1880  TGATCA               T^GATCA
             1902  TGATCA              
BsaI          577  GAGACC               GGTCTC(1/5)
             1638  GGTCTC              
BsgI         1132  CTGCAC               GTGCAG(16/14)
              589  GTGCAG              
BsiYI        1524  CCTGACCTTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
             1911  CCAGGCACTGG         
BslI         1524  CCTGACCTTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
             1911  CCAGGCACTGG         
BsmFI         717  GGGAC                GGGAC(10/14)
             1769  GGGAC               
BsrDI         960  CATTGC               GCAATG(2/0)
             1749  CATTGC              
BstYI         819  AGATCC               [GA]^GATC[CT]
             1009  GGATCC              
CfoI         1620  GCGC                 GCG^C
             1706  GCGC                
DrdI         1527  GACCTTGGGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1957  GACATGTCGGTC        
FbaI         1880  TGATCA               T^GATCA
             1902  TGATCA              
HhaI         1620  GCGC                 GCG^C
             1706  GCGC                
HinP1I       1620  GCGC                 G^CGC
             1706  GCGC                
MflI          819  AGATCC               [GA]^GATC[CT]
             1009  GGATCC              
MslI         1749  CATTGCCGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1968  CACAAGAGTG          
MspA1I        155  CAGCTG               C[AC]G^C[GT]G
              690  CAGCTG              
NcoI          238  CCATGG               C^CATGG
              859  CCATGG              
NspI          708  ACATGC               [GA]CATG^[CT]
             1958  ACATGT              
PstI          152  CTGCAG               CTGCA^G
             1021  CTGCAG              
PvuII         155  CAGCTG               CAG^CTG
              690  CAGCTG              
XhoII         819  AGATCC               [GA]^GATC[CT]
             1009  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   61 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        AvaII       BbsI        BbvI        BfaI        
BpmI        BpuAI       BsaJI       BseRI       BsmAI       
BsrI        BssKI       BstNI       Cac8I       Cfr13I      
DdeI        DpnII       DsaI        EaeI        EarI        
EcoRII      EcoT14I     Fnu4HI      HaeIII      HapII       
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeIII      MboI        MboII       MnlI        
MseI        MspI        MvaI        MwoI        NdeII       
NlaIII      NlaIV       PleI        RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        StyI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       
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