Restriction map for pF1KB4590
 (Linear) (Six-base) MAPSORT of: KIBB4590.seq  Check: 7609  from: 1  to: 2019



KIBB4590.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:26  ..

AccI GT'mk_AC

Cuts at:      0     174    2019 
   Size:        174    1845
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      29      47     407     928     935    2019 
   Size:         29      18     360     521       7    1084

  Fragments arranged by size: 

                1084     521     360      29      18       7
 
AflII C'TTAA_G

Cuts at:      0     244    2019 
   Size:        244    1775
 
AflIII A'CryG_T

Cuts at:      0    1958    2019 
   Size:       1958      61
 
AleI CACnn'nnGTG

Cuts at:      0    1972    2019 
   Size:       1972      47
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     977    2019 
   Size:        977    1042
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1917    2019 
   Size:       1917     102
 
ApoI r'AATT_y

Cuts at:      0    1078    1340    1540    1847    2019 
   Size:       1078     262     200     307     172

  Fragments arranged by size: 

                1078     307     262     200     172
 
BamHI G'GATC_C

Cuts at:      0    1009    2019 
   Size:       1009    1010
 
BanI G'GyrC_C

Cuts at:      0     679    2019 
   Size:        679    1340
 
BanII G_rGCy'C

Cuts at:      0    1662    2019 
   Size:       1662     357
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     104     551     646    2019 
   Size:        104     447      95    1373

  Fragments arranged by size: 

                1373     447     104      95
 
BbvCI CC'TCA_GC

Cuts at:      0    2003    2019 
   Size:       2003      16
 
BclI T'GATC_A

Cuts at:      0    1880    1902    2019 
   Size:       1880      22     117

  Fragments arranged by size: 

                1880     117      22
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1170    2019 
   Size:       1170     849
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     721    1481    1626    2019 
   Size:        721     760     145     393

  Fragments arranged by size: 

                 760     721     393     145
 
Bpu10I CC'TnA_GC

Cuts at:      0     374     800    1922    2003    2019 
   Size:        374     426    1122      81      16

  Fragments arranged by size: 

                1122     426     374      81      16
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     571    1644    2019 
   Size:        571    1073     375

  Fragments arranged by size: 

                1073     571     375
 
BsaBI GATnn'nnATC

Cuts at:      0    1901    2019 
   Size:       1901     118
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     139     302     575    2019 
   Size:        139     163     273    1444

  Fragments arranged by size: 

                1444     273     163     139
 
BseYI C'CCAG_C

Cuts at:      0     140    1260    1711    2019 
   Size:        140    1120     451     308

  Fragments arranged by size: 

                1120     451     308     140
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     610    1117    2019 
   Size:        610     507     902

  Fragments arranged by size: 

                 902     610     507
 
BsiHKAI G_wGCw'C

Cuts at:      0    1662    2019 
   Size:       1662     357
 
Bsp1286I G_dGCh'C

Cuts at:      0    1662    2019 
   Size:       1662     357
 
BspHI T'CATG_A

Cuts at:      0    1905    2019 
   Size:       1905     114
 
BsrDI GCAATG_nn'

Cuts at:      0     959    1748    2019 
   Size:        959     789     271

  Fragments arranged by size: 

                 959     789     271
 
BsrFI r'CCGG_y

Cuts at:      0    1380    2019 
   Size:       1380     639
 
BstYI r'GATC_y

Cuts at:      0     819    1009    2019 
   Size:        819     190    1010

  Fragments arranged by size: 

                1010     819     190
 
BtgI C'CryG_G

Cuts at:      0     238     859    1754    2019 
   Size:        238     621     895     265

  Fragments arranged by size: 

                 895     621     265     238
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1958    1993    2019 
   Size:       1958      35      26

  Fragments arranged by size: 

                1958      35      26
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1533    1963    2019 
   Size:       1533     430      56

  Fragments arranged by size: 

                1533     430      56
 
EaeI y'GGCC_r

Cuts at:      0     275     969    1324    1554    2019 
   Size:        275     694     355     230     465

  Fragments arranged by size: 

                 694     465     355     275     230
 
EarI CTCTTCn'nnn_

Cuts at:      0     181     515    1109    2019 
   Size:        181     334     594     910

  Fragments arranged by size: 

                 910     594     334     181
 
EcoICRI GAG'CTC

Cuts at:      0    1660    2019 
   Size:       1660     359
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      29      47     407     721     928     935    1481    1626 
   Size:         29      18     360     314     207       7     546     145

Cuts at:   1626    2019 
   Size:        393

  Fragments arranged by size: 

                 546     393     360     314     207     145      29      18
                   7
 
EcoRI G'AATT_C

Cuts at:      0    1078    2019 
   Size:       1078     941
 
EcoRV GAT'ATC

Cuts at:      0     921    2019 
   Size:        921    1098
 
HaeII r_GCGC'y

Cuts at:      0    1709    2019 
   Size:       1709     310
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     604     636     949     981     984    1016    1292    1324 
   Size:        604      32     313      32       3      32     276      32

Cuts at:   1324    2019 
   Size:        695

  Fragments arranged by size: 

                 695     604     313     276      32      32      32      32
                   3
 
HincII GTy'rAC

Cuts at:      0    1218    2019 
   Size:       1218     801
 
MfeI C'AATT_G

Cuts at:      0     907    2019 
   Size:        907    1112
 
MscI TGG'CCA

Cuts at:      0     277    2019 
   Size:        277    1742
 
MslI CAynn'nnrTG

Cuts at:      0    1753    1972    2019 
   Size:       1753     219      47

  Fragments arranged by size: 

                1753     219      47
 
MspA1I CmG'CkG

Cuts at:      0     157     692    2019 
   Size:        157     535    1327

  Fragments arranged by size: 

                1327     535     157
 
NcoI C'CATG_G

Cuts at:      0     238     859    2019 
   Size:        238     621    1160

  Fragments arranged by size: 

                1160     621     238
 
NspI r_CATG'y

Cuts at:      0     712    1962    2019 
   Size:        712    1250      57

  Fragments arranged by size: 

                1250     712      57
 
PciI A'CATG_T

Cuts at:      0    1958    2019 
   Size:       1958      61
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1917    2019 
   Size:       1917     102
 
PfoI T'CCnGG_A

Cuts at:      0    1642    2019 
   Size:       1642     377
 
PshAI GACnn'nnGTC

Cuts at:      0    1682    2019 
   Size:       1682     337
 
PstI C_TGCA'G

Cuts at:      0     156    1025    2019 
   Size:        156     869     994

  Fragments arranged by size: 

                 994     869     156
 
PvuII CAG'CTG

Cuts at:      0     157     692    2019 
   Size:        157     535    1327

  Fragments arranged by size: 

                1327     535     157
 
SacI G_AGCT'C

Cuts at:      0    1662    2019 
   Size:       1662     357
 
SbfI CC_TGCA'GG

Cuts at:      0    1025    2019 
   Size:       1025     994
 
SfcI C'TryA_G

Cuts at:      0     152    1021    1482    1548    2019 
   Size:        152     869     461      66     471

  Fragments arranged by size: 

                 869     471     461     152      66
 
SmlI C'TyrA_G

Cuts at:      0     244    2019 
   Size:        244    1775
 
StyI C'CwwG_G

Cuts at:      0      68     238     581     713     859    1232    1529    2019 
   Size:         68     170     343     132     146     373     297     490

  Fragments arranged by size: 

                 490     373     343     297     170     146     132      68
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1576    1953    2019 
   Size:       1576     377      66

  Fragments arranged by size: 

                1576     377      66
 
XbaI T'CTAG_A

Cuts at:      0    1650    2019 
   Size:       1650     369
 
XmnI GAAnn'nnTTC

Cuts at:      0     350    2019 
   Size:        350    1669
 

 Enzymes that do cut:

     AccI     AcuI    AflII   AflIII     AleI     AlfI    AlwNI     ApoI
    BamHI     BanI    BanII     BbsI    BbvCI     BclI     BmrI     BpmI
   Bpu10I     BsaI    BsaBI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I
    BspHI    BsrDI    BsrFI    BstYI     BtgI    CspCI     DrdI     EaeI
     EarI  EcoICRI  Eco57MI    EcoRI    EcoRV    HaeII    Hin4I   HincII
     MfeI     MscI     MslI   MspA1I     NcoI     NspI     PciI    PflMI
     PfoI    PshAI     PstI    PvuII     SacI     SbfI     SfcI     SmlI
     StyI    TaqII     XbaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI     AgeI     AhdI     AloI
     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI
     BbeI     BcgI    BciVI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmtI     BplI    BpuEI    BsaAI    BsaHI    BsaWI    BsaXI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspMI    BsrBI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
    BtgZI     BtsI     ClaI     DraI   DraIII     EagI     EciI    EcoNI
 EcoO109I     FalI     FseI     FspI    FspAI  HindIII     HpaI     KasI
     KpnI     MluI     MmeI     NaeI     NarI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI     PmeI     PmlI     PpiI
    PpuMI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII    SacII
     SalI    SanDI     SapI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI     TatI     TstI  Tth111I     XcmI     XhoI     XmaI     ZraI
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