Restriction map for pF1KB4571
##################################
# NO CUT ENZYMES (  137 enzymes) #
##################################

AatII       Acc65I      AclI        AflII       AgeI        
AhdI        Alw44I      AlwNI       AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspHI       AspI        
AvaI        AviII       AvrII       BamHI       BanI        
BanII       BbeI        BbrPI       BbvCI       BbvI        
BcgI        BciVI       BclI        BfrI        BglI        
BglII       BlnI        BlpI        BpmI        Bpu1102I    
BsaBI       BsaI        BsgI        BsiHKAI     BsiWI       
BsmBI       BsoBI       BspDI       BspHI       BspLU11I    
BspMI       BsrBI       BsrDI       BsrFI       BssHII      
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstZ17I     Bsu36I      CelII       Cfr10I      ClaI        
CpoI        DraII       DraIII      DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO109I    EcoO65I     EcoRI       EcoRV       
FbaI        FseI        FspI        HpaI        KasI        
KpnI        KspI        MamI        MluI        NaeI        
NarI        NdeI        NgoMIV      NheI        NotI        
NruI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PvuI        
PvuII       RcaI        RsrII       SacI        SacII       
SapI        SbfI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspI        StuI        SwaI        TseI        
Tth111I     Van91I      XbaI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AccII        1237  CGCG                 CG^CG
AccIII       1532  TCCGGA               T^CCGGA
AcyI          221  GACGCC               G[GA]^CG[CT]C
AflIII        962  ACGTGT               A^C[GA][CT]GT
BfaI         1603  CTAG                 C^TAG
BsaAI         961  TACGTG               [CT]AC^GT[GA]
BsaHI         221  GACGCC               G[GA]^CG[CT]C
BsaWI        1532  TCCGGA               [AT]^CCGG[AT]
BseAI        1532  TCCGGA               T^CCGGA
BsiEI         261  CGGTCG               CG[GA][CT]^CG
BsmAI        1376  GAGAC                GTCTC(1/5)
BsmFI        1674  GTCCC                GGGAC(10/14)
BsmI         1528  GCATTC               GAATGC(1/-1)
Bsp1407I      965  TGTACA               T^GTACA
BspEI        1532  TCCGGA               T^CCGGA
BsrGI         965  TGTACA               T^GTACA
BssSI        1754  CTCGTG               CACGAG(-5/-1)
BstUI        1237  CGCG                 CG^CG
BstXI        1637  CCAATTACTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         708  AGATCC               [GA]^GATC[CT]
DraI         1566  TTTAAA               TTT^AAA
DsaI         1331  CCATGG               C^C[GA][CT]GG
EcoT22I      1526  ATGCAT               ATGCA^T
FauI          281  CCCGC                CCCGC(4/6)
Fnu4HI        994  GCGGC                GC^[ACGT]GC
HaeII        1050  AGCGCC               [GA]GCGC^[CT]
Hin1I         221  GACGCC               G[GA]^CG[CT]C
HindIII      1555  AAGCTT               A^AGCTT
ItaI          994  GCGGC                GC^[ACGT]GC
MaeI         1603  CTAG                 C^TAG
MfeI          827  CAATTG               C^AATTG
MflI          708  AGATCC               [GA]^GATC[CT]
MroI         1532  TCCGGA               T^CCGGA
MslI         1432  CATCCAAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        355  CCGCTG               C[AC]G^C[GT]G
MunI          827  CAATTG               C^AATTG
MvnI         1237  CGCG                 CG^CG
NcoI         1331  CCATGG               C^CATGG
NsiI         1526  ATGCAT               ATGCA^T
NspI         1034  ACATGC               [GA]CATG^[CT]
PstI          917  CTGCAG               CTGCA^G
SalI          263  GTCGAC               G^TCGAC
ScaI         1802  AGTACT               AGT^ACT
SexAI         636  ACCTGGT              A^CC[AT]GGT
SfcI          917  CTGCAG               C^T[GA][CT]AG
SspBI         965  TGTACA               T^GTACA
XcmI         1344  CCACGATGGATTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         708  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AccI          263  GTCGAC               GT^[AC][GT]AC
              781  GTCTAC              
AvaII        1233  GGACC                G^G[AT]CC
             1673  GGTCC               
BalI         1253  TGGCCA               TGG^CCA
             1450  TGGCCA              
BbsI          869  GAAGAC               GAAGAC(2/6)
              716  GTCTTC              
BmyI          278  GTGCCC               G[AGT]GC[ACT]^C
             1816  GTGCCC              
BpuAI         869  GAAGAC               GAAGAC(2/6)
              716  GTCTTC              
BseRI         731  CTCCTC               GAGGAG(10/8)
              629  GAGGAG              
Bsp1286I      278  GTGCCC               G[AGT]GC[ACT]^C
             1816  GTGCCC              
Cfr13I       1233  GGACC                G^G[ACGT]CC
             1673  GGTCC               
HgaI          221  GACGC                GACGC(5/10)
              427  GACGC               
HincII        263  GTCGAC               GT[CT]^[GA]AC
             1323  GTTGAC              
HindII        263  GTCGAC               GT[CT]^[GA]AC
             1323  GTTGAC              
MaeII         560  ACGT                 A^CGT
              962  ACGT                
MluNI        1253  TGGCCA               TGG^CCA
             1450  TGGCCA              
MscI         1253  TGGCCA               TGG^CCA
             1450  TGGCCA              
Sau96I       1233  GGACC                G^G[ACGT]CC
             1673  GGTCC               
SmlI          874  CTCAAG               C^T[CT][GA]AG
             1017  CTTGAG              


#########################################
# 3 OR MORE CUT ENZYMES (   56 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        BcnI        BsaJI       BsiYI       BslI        
BsrI        BssKI       BstF5I      BstNI       Cac8I       
CfoI        DdeI        DpnII       EaeI        EarI        
EcoRII      EcoT14I     FokI        HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       HphI        
Ksp632I     MaeIII      MboI        MboII       MnlI        
MseI        MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PleI        RsaI        
Sau3AI      ScrFI       SfaNI       StyI        TaqI        
TfiI        Tru9I       Tsp45I      Tsp509I     TspRI       
TthHB8I     
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com