Restriction map for pF1KB4523
 (Linear) (Six-base) MAPSORT of: KIBB4523.seq  Check: 6169  from: 1  to: 1515



KIBB4523.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:26  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     211     959    1515 
   Size:        211     748     556

  Fragments arranged by size: 

                 748     556     211
 
AccI GT'mk_AC

Cuts at:      0     548    1515 
   Size:        548     967
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1169    1515 
   Size:       1169     346
 
AflIII A'CryG_T

Cuts at:      0     131     133    1515 
   Size:        131       2    1382

  Fragments arranged by size: 

                1382     131       2
 
AgeI A'CCGG_T

Cuts at:      0     884    1515 
   Size:        884     631
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1008    1515 
   Size:       1008     507
 
AleI CACnn'nnGTG

Cuts at:      0     831    1515 
   Size:        831     684
 
AlwNI CAG_nnn'CTG

Cuts at:      0      93     483     571    1090    1353    1515 
   Size:         93     390      88     519     263     162

  Fragments arranged by size: 

                 519     390     263     162      93      88
 
ApaI G_GGCC'C

Cuts at:      0      77     370    1515 
   Size:         77     293    1145

  Fragments arranged by size: 

                1145     293      77
 
ApaLI G'TGCA_C

Cuts at:      0     105    1515 
   Size:        105    1410
 
AseI AT'TA_AT

Cuts at:      0    1169    1515 
   Size:       1169     346
 
AvaI C'yCGr_G

Cuts at:      0      77    1216    1515 
   Size:         77    1139     299

  Fragments arranged by size: 

                1139     299      77
 
BanI G'GyrC_C

Cuts at:      0     321     817     970    1515 
   Size:        321     496     153     545

  Fragments arranged by size: 

                 545     496     321     153
 
BanII G_rGCy'C

Cuts at:      0      77     259     370    1515 
   Size:         77     182     111    1145

  Fragments arranged by size: 

                1145     182     111      77
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1480    1508    1515 
   Size:       1480      28       7

  Fragments arranged by size: 

                1480      28       7
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     291     325    1515 
   Size:        291      34    1190

  Fragments arranged by size: 

                1190     291      34
 
BclI T'GATC_A

Cuts at:      0    1344    1515 
   Size:       1344     171
 
BlpI GC'TnA_GC

Cuts at:      0    1489    1515 
   Size:       1489      26
 
Bme1580I G_kGCm'C

Cuts at:      0      77     109     326     370     973    1304    1515 
   Size:         77      32     217      44     603     331     211

  Fragments arranged by size: 

                 603     331     217     211      77      44      32
 
BmgBI CAC'GTC

Cuts at:      0    1506    1515 
   Size:       1506       9
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     320    1515 
   Size:        320    1195
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     103     520     696    1277    1515 
   Size:        103     417     176     581     238

  Fragments arranged by size: 

                 581     417     238     176     103
 
Bpu10I CC'TnA_GC

Cuts at:      0     253     347     576    1515 
   Size:        253      94     229     939

  Fragments arranged by size: 

                 939     253     229      94
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     322     336    1515 
   Size:        322      14    1179

  Fragments arranged by size: 

                1179     322      14
 
BsaAI yAC'GTr

Cuts at:      0     134    1515 
   Size:        134    1381
 
BsaWI w'CCGG_w

Cuts at:      0     884    1515 
   Size:        884     631
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     541     571     669     699    1171    1201    1515 
   Size:        541      30      98      30     472      30     314

  Fragments arranged by size: 

                 541     472     314      98      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      12    1274    1515 
   Size:         12    1262     241

  Fragments arranged by size: 

                1262     241      12
 
BseYI C'CCAG_C

Cuts at:      0    1484    1515 
   Size:       1484      31
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     937    1515 
   Size:        937     578
 
BsiEI CG_ry'CG

Cuts at:      0      14    1515 
   Size:         14    1501
 
BsiHKAI G_wGCw'C

Cuts at:      0     109     191     259     353     860    1515 
   Size:        109      82      68      94     507     655

  Fragments arranged by size: 

                 655     507     109      94      82      68
 
Bsp1286I G_dGCh'C

Cuts at:      0      77     109     191     259     326     353     370     860 
   Size:         77      32      82      68      67      27      17     490

Cuts at:    860     973    1304    1515 
   Size:        113     331     211

  Fragments arranged by size: 

                 490     331     211     113      82      77      68      67
                  32      27      17
 
BspHI T'CATG_A

Cuts at:      0     852    1077    1515 
   Size:        852     225     438

  Fragments arranged by size: 

                 852     438     225
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     211     275     959    1515 
   Size:        211      64     684     556

  Fragments arranged by size: 

                 684     556     211      64
 
BsrDI GCAATG_nn'

Cuts at:      0    1103    1515 
   Size:       1103     412
 
BsrFI r'CCGG_y

Cuts at:      0      55     394     884    1515 
   Size:         55     339     490     631

  Fragments arranged by size: 

                 631     490     339      55
 
BssSI C'ACGA_G

Cuts at:      0     713    1515 
   Size:        713     802
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     113     195    1515 
   Size:        113      82    1320

  Fragments arranged by size: 

                1320     113      82
 
BstEII G'GTnAC_C

Cuts at:      0     331     581    1515 
   Size:        331     250     934

  Fragments arranged by size: 

                 934     331     250
 
BstYI r'GATC_y

Cuts at:      0     495     540    1515 
   Size:        495      45     975

  Fragments arranged by size: 

                 975     495      45
 
Bsu36I CC'TnA_GG

Cuts at:      0     564    1515 
   Size:        564     951
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     375    1515 
   Size:        375    1140
 
BtsI GCAGTG_nn'

Cuts at:      0     279    1393    1515 
   Size:        279    1114     122

  Fragments arranged by size: 

                1114     279     122
 
DraI TTT'AAA

Cuts at:      0    1194    1515 
   Size:       1194     321
 
DraIII CAC_nnn'GTG

Cuts at:      0     195    1515 
   Size:        195    1320
 
EaeI y'GGCC_r

Cuts at:      0      11    1515 
   Size:         11    1504
 
EagI C'GGCC_G

Cuts at:      0      11    1515 
   Size:         11    1504
 
EarI CTCTTCn'nnn_

Cuts at:      0     714    1515 
   Size:        714     801
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      19    1515 
   Size:         19    1496
 
EcoICRI GAG'CTC

Cuts at:      0     257    1515 
   Size:        257    1258
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     103     520     696    1169    1277    1515 
   Size:        103     417     176     473     108     238

  Fragments arranged by size: 

                 473     417     238     176     108     103
 
EcoO109I rG'GnC_Cy

Cuts at:      0      74     149     264     366     863    1515 
   Size:         74      75     115     102     497     652

  Fragments arranged by size: 

                 652     497     115     102      75      74
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     939    1515 
   Size:        939     576
 
FspI TGC'GCA

Cuts at:      0     199     271    1465    1515 
   Size:        199      72    1194      50

  Fragments arranged by size: 

                1194     199      72      50
 
FspAI rTGC'GCAy

Cuts at:      0     199    1515 
   Size:        199    1316
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     340     372     847     879     994    1026    1515 
   Size:        340      32     475      32     115      32     489

  Fragments arranged by size: 

                 489     475     340     115      32      32      32
 
MfeI C'AATT_G

Cuts at:      0     899    1515 
   Size:        899     616
 
MslI CAynn'nnrTG

Cuts at:      0     458     831    1024    1171    1515 
   Size:        458     373     193     147     344

  Fragments arranged by size: 

                 458     373     344     193     147
 
MspA1I CmG'CkG

Cuts at:      0      62     113     674     831    1281    1515 
   Size:         62      51     561     157     450     234

  Fragments arranged by size: 

                 561     450     234     157      62      51
 
NaeI GCC'GGC

Cuts at:      0      57     396    1515 
   Size:         57     339    1119

  Fragments arranged by size: 

                1119     339      57
 
NgoMIV G'CCGG_C

Cuts at:      0      55     394    1515 
   Size:         55     339    1121

  Fragments arranged by size: 

                1121     339      55
 
NspI r_CATG'y

Cuts at:      0    1044    1515 
   Size:       1044     471
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     483    1376    1515 
   Size:        483     893     139

  Fragments arranged by size: 

                 893     483     139
 
PfoI T'CCnGG_A

Cuts at:      0     536    1515 
   Size:        536     979
 
PmlI CAC'GTG

Cuts at:      0     134    1515 
   Size:        134    1381
 
PpuMI rG'GwC_Cy

Cuts at:      0     264     863    1515 
   Size:        264     599     652

  Fragments arranged by size: 

                 652     599     264
 
PspOMI G'GGCC_C

Cuts at:      0      73     366    1515 
   Size:         73     293    1149

  Fragments arranged by size: 

                1149     293      73
 
PvuII CAG'CTG

Cuts at:      0     113     831    1281    1515 
   Size:        113     718     450     234

  Fragments arranged by size: 

                 718     450     234     113
 
SacI G_AGCT'C

Cuts at:      0     259    1515 
   Size:        259    1256
 
SanDI GG'GwC_CC

Cuts at:      0     863    1515 
   Size:        863     652
 
ScaI AGT'ACT

Cuts at:      0    1154    1515 
   Size:       1154     361
 
SmaI CCC'GGG

Cuts at:      0      79    1515 
   Size:         79    1436
 
SmlI C'TyrA_G

Cuts at:      0     315     337    1515 
   Size:        315      22    1178

  Fragments arranged by size: 

                1178     315      22
 
SspI AAT'ATT

Cuts at:      0     704    1515 
   Size:        704     811
 
StyI C'CwwG_G

Cuts at:      0     783     975    1515 
   Size:        783     192     540

  Fragments arranged by size: 

                 783     540     192
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     292     988    1515 
   Size:        292     696     527

  Fragments arranged by size: 

                 696     527     292
 
TatI w'GTAC_w

Cuts at:      0    1152    1515 
   Size:       1152     363
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     669     701    1171    1203    1515 
   Size:        669      32     470      32     312

  Fragments arranged by size: 

                 669     470     312      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     171     219     982    1056    1137    1515 
   Size:        171      48     763      74      81     378

  Fragments arranged by size: 

                 763     378     171      81      74      48
 
XmaI C'CCGG_G

Cuts at:      0      77    1515 
   Size:         77    1438
 
XmnI GAAnn'nnTTC

Cuts at:      0     241     703    1515 
   Size:        241     462     812

  Fragments arranged by size: 

                 812     462     241
 

 Enzymes that do cut:

     AarI     AccI     AcuI   AflIII     AgeI     AhdI     AleI    AlwNI
     ApaI    ApaLI     AseI     AvaI     BanI    BanII     BbsI     BcgI
     BclI     BlpI Bme1580I    BmgBI     BmrI     BpmI   Bpu10I    BpuEI
    BsaAI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
 Bsp1286I    BspHI    BspMI    BsrDI    BsrFI    BssSI   BstAPI   BstEII
    BstYI   Bsu36I    BtgZI     BtsI     DraI   DraIII     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI EcoO109I     FalI     FspI    FspAI
    Hin4I     MfeI     MslI   MspA1I     NaeI   NgoMIV     NspI    PflMI
     PfoI     PmlI    PpuMI   PspOMI    PvuII     SacI    SanDI     ScaI
     SmaI     SmlI     SspI     StyI    TaqII     TatI     TstI     XcmI
     XmaI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII     AlfI     AloI     ApoI
     AscI    AsiSI    AvrII     BaeI    BamHI     BbeI    BbvCI    BciVI
    BfrBI     BglI    BglII     BmtI     BplI     BsaI    BsaBI    BsaHI
    BsiWI     BsmI    BsmBI    BspEI    BsrBI    BsrGI   BssHII    BstBI
    BstXI  BstZ17I     BtgI     ClaI    CspCI     DrdI    EcoNI    EcoRI
    EcoRV     FseI    HaeII   HincII  HindIII     HpaI     KasI     KpnI
     MluI     MmeI     MscI     NarI     NcoI     NdeI     NheI     NotI
     NruI     NsiI     PacI     PasI     PciI     PmeI     PpiI    PshAI
     PsiI    PspXI     PsrI     PstI     PvuI    RsrII    SacII     SalI
     SapI     SbfI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
    SnaBI     SpeI     SphI     SrfI     StuI     SwaI  Tth111I     XbaI
     XhoI     ZraI
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