Restriction map for pF1KB4495
##################################
# NO CUT ENZYMES (  105 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AflII       AflIII      AgeI        AhdI        AscI        
AseI        AsnI        Asp718I     AspEI       AviII       
AvrII       BbeI        BbsI        BciVI       BclI        
BfrI        BlnI        BpuAI       BseAI       BsiEI       
BsiWI       BsmI        BspDI       BspEI       BspHI       
BspLU11I    BsrDI       BssHII      Bst1107I    BstEII      
BstPI       BstUI       BstZ17I     ClaI        CpoI        
DraI        DrdI        EagI        Eam1105I    EarI        
EclXI       Eco52I      EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        FseI        FspI        HincII      
HindII      HindIII     HpaI        KasI        KpnI        
Ksp632I     KspI        MfeI        MluI        MroI        
MunI        MvnI        NaeI        NarI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
PacI        PaeR7I      PinAI       PmeI        PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacII       SalI        SapI        SbfI        SexAI       
SfiI        SfoI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspI        StuI        
SwaI        XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AccI          142  GTAGAC               GT^[AC][GT]AC
AcsI         2245  AAATTC               [GA]^AATT[CT]
Alw44I       2140  GTGCAC               G^TGCAC
AocI         1245  CCTGAGG              CC^T[ACGT]AGG
ApaLI        2140  GTGCAC               G^TGCAC
ApoI         2245  AAATTC               [GA]^AATT[CT]
Asp700I      2451  GAAGTCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI         1306  GACACGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BamHI          14  GGATCC               G^GATCC
BanI         1536  GGCACC               G^G[CT][GA]CC
BbrPI        1018  CACGTG               CAC^GTG
BbvCI        1825  CCTCAGC              CCTCAGC(-5/-2)
BcgI          641  GCAACATCTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI         2404  CTAG                 C^TAG
BglII        1058  AGATCT               A^GATCT
BsaBI         180  GATTGCGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI         341  ACCGGA               [AT]^CCGG[AT]
BsmBI          94  GAGACG               CGTCTC(1/5)
Bsp1407I      464  TGTACA               T^GTACA
BsrGI         464  TGTACA               T^GTACA
BssSI        2137  CTCGTG               CACGAG(-5/-1)
BstAPI       2226  GCAGATCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI         848  TTCGAA               TT^CGAA
Bsu36I       1245  CCTGAGG              CC^T[ACGT]AGG
Eco81I       1245  CCTGAGG              CC^T[ACGT]AGG
EcoNI        2267  CCTCCGTGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
MaeI         2404  CTAG                 C^TAG
MamI          180  GATTGCGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI          104  TTAA                 T^TAA
NcoI         1365  CCATGG               C^CATGG
NdeI         1271  CATATG               CA^TATG
NspV          848  TTCGAA               TT^CGAA
PflFI        1306  GACACGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI        1018  CACGTG               CAC^GTG
PmlI         1018  CACGTG               CAC^GTG
ScaI          562  AGTACT               AGT^ACT
SfuI          848  TTCGAA               TT^CGAA
SspBI         464  TGTACA               T^GTACA
Tru9I         104  TTAA                 T^TAA
Tth111I      1306  GACACGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XmnI         2451  GAAGTCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (    8 enzymes) #
###################################

ApaI         2171  GGGCCC               GGGCC^C
             2193  GGGCCC              
BalI          909  TGGCCA               TGG^CCA
             2591  TGGCCA              
BsrFI         690  GCCGGT               [GA]^CCGG[CT]
              799  ACCGGC              
Cfr10I        690  GCCGGT               [GA]^CCGG[CT]
              799  ACCGGC              
DraIII        353  CACTGAGTG            CAC[ACGT][ACGT][ACGT]^GTG
             1392  CACCGTGTG           
MluNI         909  TGGCCA               TGG^CCA
             2591  TGGCCA              
MscI          909  TGGCCA               TGG^CCA
             2591  TGGCCA              
SacI         1093  GAGCTC               GAGCT^C
             1747  GAGCTC              


#########################################
# 3 OR MORE CUT ENZYMES (  104 enzymes) #
#########################################

AciI        AcyI        AfaI        AluI        AlwI        
AlwNI       Aor51HI     AspHI       AvaI        AvaII       
BanII       BbvI        BcnI        BglI        BlpI        
BmyI        BpmI        Bpu1102I    BsaAI       BsaHI       
BsaI        BsaJI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BspMI       BsrBI       BsrI        BssKI       
BstF5I      BstNI       BstXI       BstYI       Cac8I       
CelII       CfoI        Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        Eco47III    EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        MaeII       MaeIII      MboI        MboII       
MflI        MnlI        MslI        MspA1I      MspI        
MvaI        MwoI        NciI        NdeII       NlaIII      
NlaIV       PflMI       PleI        PpuMI       PstI        
PvuII       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        StyI        TaqI        
TfiI        TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     Van91I      XcmI        XhoII       
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