Restriction map for pF1KB4477
##################################
# NO CUT ENZYMES (   89 enzymes) #
##################################

AccI        AccII       AccIII      AclI        AcsI        
AflII       AgeI        ApoI        AscI        AseI        
AsnI        AspI        AviII       AvrII       BamHI       
BbrPI       BbvCI       BcgI        BciVI       BfrI        
BlnI        BsaAI       BsaBI       BseAI       BsiWI       
BsmI        BspDI       BspEI       BspLU11I    BssHII      
Bst1107I    BstBI       BstUI       BstZ17I     ClaI        
CpoI        DraI        DrdI        EcoRI       EcoRV       
EcoT22I     FseI        FspI        HpaI        KspI        
MamI        MfeI        MluI        MroI        MseI        
MunI        MvnI        NdeI        NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
SfiI        SfuI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspI        StuI        SwaI        
Tru9I       Tth111I     XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

AatII        1318  GACGTC               GACGT^C
Acc65I        437  GGTACC               G^GTACC
AflIII       3248  ACACGT               A^C[GA][CT]GT
Alw44I       2636  GTGCAC               G^TGCAC
Aor51HI      2189  AGCGCT               AGC^GCT
ApaI         2658  GGGCCC               GGGCC^C
ApaLI        2636  GTGCAC               G^TGCAC
Asp718I       437  GGTACC               G^GTACC
BbeI         3130  GGCGCC               GGCGC^C
BclI         1865  TGATCA               T^GATCA
BsaI         2102  GGTCTC               GGTCTC(1/5)
BsaWI        2099  TCCGGT               [AT]^CCGG[AT]
BsiEI        2822  CGGCCG               CG[GA][CT]^CG
BsmBI         612  CGTCTC               CGTCTC(1/5)
Bsp1407I     1079  TGTACA               T^GTACA
BsrGI        1079  TGTACA               T^GTACA
BssSI        2859  CACGAG               CACGAG(-5/-1)
BstAPI       2767  GCAGCCTCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
EagI         2822  CGGCCG               C^GGCCG
EclXI        2822  CGGCCG               C^GGCCG
Eco47III     2189  AGCGCT               AGC^GCT
Eco52I       2822  CGGCCG               C^GGCCG
EcoNI        1389  CCTGTCCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI         1865  TGATCA               T^GATCA
HincII        412  GTCAAC               GT[CT]^[GA]AC
HindII        412  GTCAAC               GT[CT]^[GA]AC
HindIII        45  AAGCTT               A^AGCTT
KasI         3130  GGCGCC               G^GCGCC
KpnI          437  GGTACC               GGTAC^C
NarI         3130  GGCGCC               GG^CGCC
PflMI        1262  CCATCAAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ScaI           21  AGTACT               AGT^ACT
SfoI         3130  GGCGCC               GGC^GCC
SgrAI        2154  CACCGGCG             C[GA]^CCGG[CT]G
SspBI        1079  TGTACA               T^GTACA
Van91I       1262  CCATCAAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AhdI         2272  GACTTGCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3040  GACCAGCCGTC         
AlwNI         175  CAGGGTCTG            CAG[ACGT][ACGT][ACGT]^CTG
             2768  CAGCCTCTG           
AspEI        2272  GACTTGCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3040  GACCAGCCGTC         
BglI         1436  GCCCAGAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             2824  GCCGGGGAGGC         
BlpI          477  GCTGAGC              GC^T[ACGT]AGC
             2599  GCTGAGC             
Bpu1102I      477  GCTGAGC              GC^T[ACGT]AGC
             2599  GCTGAGC             
BspHI         923  TCATGA               T^CATGA
             1007  TCATGA              
BsrBI         191  CCGCTC               CCGCTC(-3/-3)
             3115  GAGCGG              
BstEII       2379  GGTGACC              G^GT[ACGT]ACC
             2520  GGTCACC             
BstPI        2379  GGTGACC              G^GT[ACGT]ACC
             2520  GGTCACC             
CelII         477  GCTGAGC              GC^T[ACGT]AGC
             2599  GCTGAGC             
DraIII       1180  CACTTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
             2241  CACGGAGTG           
Eam1105I     2272  GACTTGCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3040  GACCAGCCGTC         
EcoO65I      2379  GGTGACC              G^GT[ACGT]ACC
             2520  GGTCACC             
HgaI         2887  GACGC                GACGC(5/10)
             1598  GCGTC               
NspI         1364  GCATGT               [GA]CATG^[CT]
             1477  GCATGT              
PvuII        2015  CAGCTG               CAG^CTG
             2597  CAGCTG              
RcaI          923  TCATGA               T^CATGA
             1007  TCATGA              
SexAI         947  ACCTGGT              A^CC[AT]GGT
             2375  ACCAGGT             
TaqI           94  TCGA                 T^CGA
             1776  TCGA                
TthHB8I        94  TCGA                 T^CGA
             1776  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (  112 enzymes) #
#########################################

AciI        AcyI        AfaI        AluI        AlwI        
AocI        Asp700I     AspHI       AvaI        AvaII       
BalI        BanI        BanII       BbsI        BbvI        
BcnI        BfaI        BglII       BmyI        BpmI        
BpuAI       BsaHI       BsaJI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BspMI       BsrDI       BsrFI       
BsrI        BssKI       BstF5I      BstNI       BstXI       
BstYI       Bsu36I      Cac8I       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EarI        Eco81I      EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HhaI        Hin1I       HinP1I      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MscI        MslI        
MspA1I      MspI        MvaI        MwoI        NaeI        
NciI        NcoI        NdeII       NgoMIV      NlaIII      
NlaIV       PleI        PpuMI       PstI        RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmaI        StyI        TfiI        TseI        Tsp45I      
Tsp509I     TspRI       XcmI        XhoII       XmaCI       
XmaI        XmnI        
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