Restriction map for pF1KB4477
 (Circular) (Six-base) MAPSORT of: pF1KB4477.seq  Check: 2151  from: 1  to: 6560



pF1KB4477  6560 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:24  ..

AatII G_ACGT'C

Cuts at:   1430    1430 
   Size:       6560
 
AccI GT'mk_AC

Cuts at:   3598    3598 
   Size:       6560
 
Acc65I G'GTAC_C

Cuts at:    545    3576     545 
   Size:       3031    3529
 
AclI AA'CG_TT

Cuts at:   6400    6400 
   Size:       6560
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    235    3439    3489    4385    4817    5170    5832     235 
   Size:       3204      50     896     432     353     662     963

  Fragments arranged by size: 

                3204     963     896     662     432     353      50
 
AfeI AGC'GCT

Cuts at:   2299    2299 
   Size:       6560
 
AflIII A'CryG_T

Cuts at:   3356    3912    5712    5993    3356 
   Size:        556    1800     281    3923

  Fragments arranged by size: 

                3923    1800     556     281
 
AhdI GACnn_n'nnGTC

Cuts at:   2385    3153    2385 
   Size:        768    5792
 
AleI CACnn'nnGTG

Cuts at:   1801    2432    1801 
   Size:        631    5929
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   1078    1110    1078 
   Size:         32    6528
 
AlwNI CAG_nnn'CTG

Cuts at:    288    2881    3865    5303     288 
   Size:       2593     984    1438    1545

  Fragments arranged by size: 

                2593    1545    1438     984
 
ApaI G_GGCC'C

Cuts at:   2770    2770 
   Size:       6560
 
ApaLI G'TGCA_C

Cuts at:   2744    5398    2744 
   Size:       2654    3906
 
ApoI r'AATT_y

Cuts at:   3564    5913    6169    3564 
   Size:       2349     256    3955

  Fragments arranged by size: 

                3955    2349     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       6560
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       6560
 
AvaI C'yCGr_G

Cuts at:   1082    1387    1485    2375    2492    2580    2646    2916    3188 
   Size:        305      98     890     117      88      66     270     272

Cuts at:   3188    3580    1082 
   Size:        392    4062

  Fragments arranged by size: 

                4062     890     392     305     272     270     117      98
                  88      66
 
BamHI G'GATC_C

Cuts at:   3585    3585 
   Size:       6560
 
BanI G'GyrC_C

Cuts at:    545    2755    3238    3576    3990    4238    4273     545 
   Size:       2210     483     338     414     248      35    2832

  Fragments arranged by size: 

                2832    2210     483     414     338     248      35
 
BanII G_rGCy'C

Cuts at:    791    1091    2181    2200    2587    2770    2917    3574    4604 
   Size:        300    1090      19     387     183     147     657    1030

Cuts at:   4604     791 
   Size:       2747

  Fragments arranged by size: 

                2747    1090    1030     657     387     300     183     147
                  19
 
BbeI G_GCGC'C

Cuts at:   3242    3994    4242    3242 
   Size:        752     248    5560

  Fragments arranged by size: 

                5560     752     248
 
BbsI GAAGACnn'nnnn_

Cuts at:    982    1996    2403    2547    3149     982 
   Size:       1014     407     144     602    4393

  Fragments arranged by size: 

                4393    1014     602     407     144
 
BciVI GTATCCnnnnn_n'

Cuts at:   4458    5514    6560    4458 
   Size:       1056    1046    4458

  Fragments arranged by size: 

                4458    1056    1046
 
BclI T'GATC_A

Cuts at:   1973    4080    1973 
   Size:       2107    4453
 
BglI GCCn_nnn'nGGC

Cuts at:   1550    2938    1550 
   Size:       1388    5172
 
BglII A'GATC_T

Cuts at:   1202    1251    3458    4075    6112    1202 
   Size:         49    2207     617    2037    1650

  Fragments arranged by size: 

                2207    2037    1650     617      49
 
BlpI GC'TnA_GC

Cuts at:    586    2708    3672     586 
   Size:       2122     964    3474

  Fragments arranged by size: 

                3474    2122     964
 
Bme1580I G_kGCm'C

Cuts at:   2100    2429    2748    2758    2770    4185    4278    5402    2100 
   Size:        329     319      10      12    1415      93    1124    3258

  Fragments arranged by size: 

                3258    1415    1124     329     319      93      12      10
            
 
BmgBI CAC'GTC

Cuts at:   1803    1803 
   Size:       6560
 
BmrI ACTGGGnnnn_n'

Cuts at:    332    3772    3853    4188    6332     332 
   Size:       3440      81     335    2144     560

  Fragments arranged by size: 

                3440    2144     560     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    555    1862    3043    4927     555 
   Size:       1307    1181    1884    2188

  Fragments arranged by size: 

                2188    1884    1307    1181
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    550    1246    1376    1752    2274    2571    2915    3203    3355 
   Size:        696     130     376     522     297     344     288     152

Cuts at:   3355    3454     550 
   Size:         99    3656

  Fragments arranged by size: 

                3656     696     522     376     344     297     288     152
                 130      99
 
Bpu10I CC'TnA_GC

Cuts at:   1315    2199    2739    1315 
   Size:        884     540    5136

  Fragments arranged by size: 

                5136     884     540
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3736    4615    5329    5627    3736 
   Size:        879     714     298    4669

  Fragments arranged by size: 

                4669     879     714     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   2216    6264    2216 
   Size:       4048    2512
 
BsaAI yAC'GTr

Cuts at:   3913    3913 
   Size:       6560
 
BsaBI GATnn'nnATC

Cuts at:      6    4074    6111       6 
   Size:       4068    2037     455

  Fragments arranged by size: 

                4068    2037     455
 
BsaHI Gr'CG_yC

Cuts at:   1427    1706    3239    3991    4239    4941    6437    1427 
   Size:        279    1533     752     248     702    1496    1550

  Fragments arranged by size: 

                1550    1533    1496     752     702     279     248
 
BsaWI w'CCGG_w

Cuts at:   2207    3749    4270    4948    5359    5506    5780    2207 
   Size:       1542     521     678     411     147     274    2987

  Fragments arranged by size: 

                2987    1542     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    709     739    1078    1108    1396    1426    1600    1630    2344 
   Size:         30     339      30     288      30     174      30     714

Cuts at:   2344    2374    2461    2491    2788    2818     709 
   Size:         30      87      30     297      30    4451

  Fragments arranged by size: 

                4451     714     339     297     288     174      87      30
                  30      30      30      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    714    2284    2287    2791    2796    2805    3505     714 
   Size:       1570       3     504       5       9     700    3769

  Fragments arranged by size: 

                3769    1570     700     504       9       5       3
 
BseYI C'CCAG_C

Cuts at:    491     906    1692    2130    2149    2161    3007    3096    3985 
   Size:        415     786     438      19      12     846      89     889

Cuts at:   3985    4743    5408    5816     491 
   Size:        758     665     408    1235

  Fragments arranged by size: 

                1235     889     846     786     758     665     438     415
                 408      89      19      12
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1062    1081    1367    3568    1062 
   Size:         19     286    2201    4054

  Fragments arranged by size: 

                4054    2201     286      19
 
BsiEI CG_ry'CG

Cuts at:    104    2933    4102    4148    5378     104 
   Size:       2829    1169      46    1230    1286

  Fragments arranged by size: 

                2829    1286    1230    1169      46
 
BsiHKAI G_wGCw'C

Cuts at:   1787    2748    3044    3574    4352    4542    5402    1787 
   Size:        961     296     530     778     190     860    2945

  Fragments arranged by size: 

                2945     961     860     778     530     296     190
 
BsmBI CGTCTCn'nnnn_

Cuts at:    726     726 
   Size:       6560
 
Bsp1286I G_dGCh'C

Cuts at:    791    1091    1787    2100    2181    2200    2429    2587    2748 
   Size:        300     696     313      81      19     229     158     161

Cuts at:   2748    2758    2770    2917    3044    3574    4185    4278    4352 
   Size:         10      12     147     127     530     611      93      74

Cuts at:   4352    4542    4604    5402     791 
   Size:        190      62     798    1949

  Fragments arranged by size: 

                1949     798     696     611     530     313     300     229
                 190     161     158     147     127      93      81      74
                  62      19      12      10
 
BspHI T'CATG_A

Cuts at:   1031    1115    4992    1031 
   Size:         84    3877    2599

  Fragments arranged by size: 

                3877    2599      84
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1310    2616    2862    3610    3824    4126    4507    1310 
   Size:       1306     246     748     214     302     381    3363

  Fragments arranged by size: 

                3363    1306     748     381     302     246     214
 
BsrBI CCG'CTC

Cuts at:     50     301    3225    4852    4906    6390    6555      50 
   Size:        251    2924    1627      54    1484     165      55

  Fragments arranged by size: 

                2924    1627    1484     251     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    231     573    1873    2160    4472     231 
   Size:        342    1300     287    2312    2319

  Fragments arranged by size: 

                2319    2312    1300     342     287
 
BsrFI r'CCGG_y

Cuts at:   1157    1610    2062    2263    2442    3340    4558    4739    1157 
   Size:        453     452     201     179     898    1218     181    2978

  Fragments arranged by size: 

                2978    1218     898     453     452     201     181     179
            
 
BsrGI T'GTAC_A

Cuts at:   1187    1187 
   Size:       6560
 
BssSI C'ACGA_G

Cuts at:   2967    4831    5539    2967 
   Size:       1864     708    3988

  Fragments arranged by size: 

                3988    1864     708
 
BstAPI GCAn_nnn'nTGC

Cuts at:   2881    3937    2881 
   Size:       1056    5504
 
BstBI TT'CG_AA

Cuts at:   4921    4921 
   Size:       6560
 
BstEII G'GTnAC_C

Cuts at:   2487    2628    2487 
   Size:        141    6419
 
BstXI CCAn_nnnn'nTGG

Cuts at:   1002    2973    3255    1002 
   Size:       1971     282    4307

  Fragments arranged by size: 

                4307    1971     282
 
BstYI r'GATC_y

Cuts at:      1    1202    1251    3458    3585    4052    4075    4410    4656 
   Size:       1201      49    2207     127     467      23     335     246

Cuts at:   4656    4962    4974    5060    6112       1 
   Size:        306      12      86    1052     449

  Fragments arranged by size: 

                2207    1201    1052     467     449     335     306     246
                 127      86      49      23      12
 
Bsu36I CC'TnA_GG

Cuts at:    281    1144    2688     281 
   Size:        863    1544    4153

  Fragments arranged by size: 

                4153    1544     863
 
BtgI C'CryG_G

Cuts at:    107     549     995    1175    1214    1710    2334    2390    2633 
   Size:        442     446     180      39     496     624      56     243

Cuts at:   2633     107 
   Size:       4034

  Fragments arranged by size: 

                4034     624     496     446     442     243     180      56
                  39
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   4510    4691    4854    4510 
   Size:        181     163    6216

  Fragments arranged by size: 

                6216     181     163
 
BtsI GCAGTG_nn'

Cuts at:    219     695    1600    2688    3335    3486    3803     219 
   Size:        476     905    1088     647     151     317    2976

  Fragments arranged by size: 

                2976    1088     905     647     476     317     151
 
DraI TTT'AAA

Cuts at:   3559    3559 
   Size:       6560
 
DraIII CAC_nnn'GTG

Cuts at:   1293    2354    1293 
   Size:       1061    5499
 
DrdI GACnn_nn'nnGTC

Cuts at:   4266    5610    4266 
   Size:       1344    5216
 
EaeI y'GGCC_r

Cuts at:    256     383     483     639     749     779     968    1193    2040 
   Size:        127     100     156     110      30     189     225     847

Cuts at:   2040    2930    3047    3314    3491    4145    4319    4710    4737 
   Size:        890     117     267     177     654     174     391      27

Cuts at:   4737    6082     256 
   Size:       1345     734

  Fragments arranged by size: 

                1345     890     847     734     654     391     267     225
                 189     177     174     156     127     117     110     100
                  30      27
 
EagI C'GGCC_G

Cuts at:   2930    4145    2930 
   Size:       1215    5345
 
EarI CTCTTCn'nnn_

Cuts at:   1001    2066    2452    3203    4583    4793    1001 
   Size:       1065     386     751    1380     210    2768

  Fragments arranged by size: 

                2768    1380    1065     751     386     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2307    3057    4768    5510    5656    6370    2307 
   Size:        750    1711     742     146     714    2497

  Fragments arranged by size: 

                2497    1711     750     742     714     146
 
EcoICRI GAG'CTC

Cuts at:   3572    3572 
   Size:       6560
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    235     550    1246    1376    1752    2274    2571    2915    3203 
   Size:        315     696     130     376     522     297     344     288

Cuts at:   3203    3355    3439    3454    3489    4385    4817    5170    5832 
   Size:        152      84      15      35     896     432     353     662

Cuts at:   5832     235 
   Size:        963

  Fragments arranged by size: 

                 963     896     696     662     522     432     376     353
                 344     315     297     288     152     130      84      35
                  15
 
EcoNI CCTnn'n_nnAGG

Cuts at:   1501    1501 
   Size:       6560
 
EcoO109I rG'GnC_Cy

Cuts at:    495    1737    1763    1897    2166    2693    2766    2767    3024 
   Size:       1242      26     134     269     527      73       1     257

Cuts at:   3024    3083    3699     495 
   Size:         59     616    3356

  Fragments arranged by size: 

                3356    1242     616     527     269     257     134      73
                  59      26       1
 
EcoRI G'AATT_C

Cuts at:   3564    3564 
   Size:       6560
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1254    1974    3426    4579    1254 
   Size:        720    1452    1153    3235

  Fragments arranged by size: 

                3235    1452    1153     720
 
FspI TGC'GCA

Cuts at:   4341    4341 
   Size:       6560
 
HaeII r_GCGC'y

Cuts at:   1545    1932    1970    2301    3242    3280    3994    4242    5472 
   Size:        387      38     331     941      38     714     248    1230

Cuts at:   5472    6243    1545 
   Size:        771    1862

  Fragments arranged by size: 

                1862    1230     941     771     714     387     331     248
                  38      38
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1600    1632    2344    2371    2376    2403    2461    2493    3139 
   Size:         32     712      27       5      27      58      32     646

Cuts at:   3139    3171    3407    3439    4402    4432    4434    4464    1600 
   Size:         32     236      32     963      30       2      30    3696

  Fragments arranged by size: 

                3696     963     712     646     236      58      32      32
                  32      32      30      30      27      27       5       2
            
 
HincII GTy'rAC

Cuts at:    522    3599     522 
   Size:       3077    3483
 
HindIII A'AGCT_T

Cuts at:    153     153 
   Size:       6560
 
KasI G'GCGC_C

Cuts at:   3238    3990    4238    3238 
   Size:        752     248    5560

  Fragments arranged by size: 

                5560     752     248
 
KpnI G_GTAC'C

Cuts at:    549    3580     549 
   Size:       3031    3529
 
MluI A'CGCG_T

Cuts at:   5993    5993 
   Size:       6560
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    286    1448    5319    5503     286 
   Size:       1162    3871     184    1343

  Fragments arranged by size: 

                3871    1343    1162     184
 
MscI TGG'CCA

Cuts at:    385     485     641    1195    3316    4321     385 
   Size:        100     156     554    2121    1005    2624

  Fragments arranged by size: 

                2624    2121    1005     554     156     100
 
MslI CAynn'nnrTG

Cuts at:    224     892    1000    1801    2432    4549    4676     224 
   Size:        668     108     801     631    2117     127    2108

  Fragments arranged by size: 

                2117    2108     801     668     631     127     108
 
MspA1I CmG'CkG

Cuts at:    255     574     593    2125    2707    3236    3490    3671    3985 
   Size:        319      19    1532     582     529     254     181     314

Cuts at:   3985    4345    5129    5374     255 
   Size:        360     784     245    1441

  Fragments arranged by size: 

                1532    1441     784     582     529     360     319     314
                 254     245     181      19
 
NaeI GCC'GGC

Cuts at:   2064    2444    3342    4741    2064 
   Size:        380     898    1399    3883

  Fragments arranged by size: 

                3883    1399     898     380
 
NarI GG'CG_CC

Cuts at:   3239    3991    4239    3239 
   Size:        752     248    5560

  Fragments arranged by size: 

                5560     752     248
 
NcoI C'CATG_G

Cuts at:    107     549     995    1214    2334     107 
   Size:        442     446     219    1120    4333

  Fragments arranged by size: 

                4333    1120     446     442     219
 
NgoMIV G'CCGG_C

Cuts at:   2062    2442    3340    4739    2062 
   Size:        380     898    1399    3883

  Fragments arranged by size: 

                3883    1399     898     380
 
NspI r_CATG'y

Cuts at:   1476    1589    3613    4113    5716    1476 
   Size:        113    2024     500    1603    2320

  Fragments arranged by size: 

                2320    2024    1603     500     113
 
PciI A'CATG_T

Cuts at:   5712    5712 
   Size:       6560
 
PflMI CCAn_nnn'nTGG

Cuts at:   1376    1376 
   Size:       6560
 
PfoI T'CCnGG_A

Cuts at:   1133    1655    1768    2027    2183    3260    3446    1133 
   Size:        522     113     259     156    1077     186    4247

  Fragments arranged by size: 

                4247    1077     522     259     186     156     113
 
PmeI GTTT'AAAC

Cuts at:   3559    3559 
   Size:       6560
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1078    1110    5006    5038    1078 
   Size:         32    3896      32    2600

  Fragments arranged by size: 

                3896    2600      32      32
 
PpuMI rG'GwC_Cy

Cuts at:    495    1763    1897    2166    2693    3083     495 
   Size:       1268     134     269     527     390    3972

  Fragments arranged by size: 

                3972    1268     527     390     269     134
 
PspOMI G'GGCC_C

Cuts at:   2766    2766 
   Size:       6560
 
PstI C_TGCA'G

Cuts at:   1029    1736    1782    2706    2886    3607    4292    1029 
   Size:        707      46     924     180     721     685    3297

  Fragments arranged by size: 

                3297     924     721     707     685     180      46
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       6560
 
PvuII CAG'CTG

Cuts at:   2125    2707    3985    4345    2125 
   Size:        582    1278     360    4340

  Fragments arranged by size: 

                4340    1278     582     360
 
RsrII CG'GwC_CG

Cuts at:   4755    4755 
   Size:       6560
 
SacI G_AGCT'C

Cuts at:   3574    3574 
   Size:       6560
 
SalI G'TCGA_C

Cuts at:   3597    3597 
   Size:       6560
 
SanDI GG'GwC_CC

Cuts at:    495    2166    2693    3083     495 
   Size:       1671     527     390    3972

  Fragments arranged by size: 

                3972    1671     527     390
 
SapI GCTCTTCn'nnn_

Cuts at:   4583    4793    4583 
   Size:        210    6350
 
SbfI CC_TGCA'GG

Cuts at:   3607    3607 
   Size:       6560
 
ScaI AGT'ACT

Cuts at:    131    6077     131 
   Size:       5946     614
 
SexAI A'CCwGG_T

Cuts at:   1055    2483    1055 
   Size:       1428    5132
 
SfcI C'TryA_G

Cuts at:     33    1025    1732    1778    2702    2882    3603    4288    5256 
   Size:        992     707      46     924     180     721     685     968

Cuts at:   5256    5447      33 
   Size:        191    1146

  Fragments arranged by size: 

                1146     992     968     924     721     707     685     191
                 180      46
 
SfoI GGC'GCC

Cuts at:   3240    3992    4240    3240 
   Size:        752     248    5560

  Fragments arranged by size: 

                5560     752     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       6560
 
SgrAI Cr'CCGG_yG

Cuts at:   2263    2263 
   Size:       6560
 
SmaI CCC'GGG

Cuts at:   1084    1487    2582    3582    1084 
   Size:        403    1095    1000    4062

  Fragments arranged by size: 

                4062    1095    1000     403
 
SmlI C'TyrA_G

Cuts at:   3715    4630    5344    5606    3715 
   Size:        915     714     262    4669

  Fragments arranged by size: 

                4669     915     714     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       6560
 
SphI G_CATG'C

Cuts at:   3613    4113    3613 
   Size:        500    6060
 
SrfI GCCC'GGGC

Cuts at:   2582    2582 
   Size:       6560
 
StyI C'CwwG_G

Cuts at:    107     314     486     549     995    1068    1214    1823    2114 
   Size:        207     172      63     446      73     146     609     291

Cuts at:   2114    2334    2342    3248    3694    4048     107 
   Size:        220       8     906     446     354    2619

  Fragments arranged by size: 

                2619     906     609     446     446     354     291     220
                 207     172     146      73      63       8
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   4143    4276    4735    4944    4143 
   Size:        133     459     209    5759

  Fragments arranged by size: 

                5759     459     209     133
 
TatI w'GTAC_w

Cuts at:    129    1187    6075     129 
   Size:       1058    4888     614

  Fragments arranged by size: 

                4888    1058     614
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2338    2370    2788    2820    6077    6109    2338 
   Size:         32     418      32    3257      32    2789

  Fragments arranged by size: 

                3257    2789     418      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   4357    4357 
   Size:       6560
 
XbaI T'CTAG_A

Cuts at:   3591    3591 
   Size:       6560
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    478    1221    3429    4055     478 
   Size:        743    2208     626    2983

  Fragments arranged by size: 

                2983    2208     743     626
 
XmaI C'CCGG_G

Cuts at:   1082    1485    2580    3580    1082 
   Size:        403    1095    1000    4062

  Fragments arranged by size: 

                4062    1095    1000     403
 
XmnI GAAnn'nnTTC

Cuts at:    248     323    1237     248 
   Size:         75     914    5571

  Fragments arranged by size: 

                5571     914      75
 
ZraI GAC'GTC

Cuts at:   1428    1428 
   Size:       6560
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI     AfeI   AflIII     AhdI
     AleI     AloI    AlwNI     ApaI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbeI     BbsI    BciVI     BclI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI    BstBI
   BstEII    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI     DraI
   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I   HincII
  HindIII     KasI     KpnI     MluI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI   NgoMIV     NspI     PciI    PflMI     PfoI
     PmeI     PpiI    PpuMI   PspOMI     PstI     PvuI    PvuII    RsrII
     SacI     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfoI     SgfI    SgrAI     SmaI     SmlI     SpeI     SphI     SrfI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI
     XmnI     ZraI

 Enzymes that do not cut: 

     AarI    AflII     AgeI     AlfI     AscI    AvrII     BaeI    BbvCI
     BcgI    BfrBI     BmtI    BsiWI     BsmI    BspEI   BssHII  BstZ17I
     ClaI    CspCI    EcoRV     FseI    FspAI     HpaI     MfeI     NdeI
     NheI     NotI     NruI     NsiI     PacI     PasI     PmlI    PshAI
     PsiI    PspXI     PsrI    SacII     SfiI    SnaBI     SspI     StuI
     SwaI     XhoI
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