Restriction map for pF1KB4431
##################################
# NO CUT ENZYMES (   73 enzymes) #
##################################

AatII       AclI        AcsI        AflII       AgeI        
Alw44I      ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     AviII       AvrII       BbeI        
BbsI        BcgI        BciVI       BclI        BfrI        
BlnI        BpuAI       BsaBI       BsmI        BspDI       
BspLU11I    BssHII      BssSI       ClaI        CpoI        
DraIII      EcoRI       EcoRV       EcoT22I     FbaI        
FseI        FspI        HincII      HindII      HpaI        
KasI        MamI        MfeI        MluI        MunI        
NarI        NheI        NotI        NruI        NsiI        
PacI        PaeR7I      PinAI       PmeI        PshAI       
PshBI       Psp1406I    PvuI        RsrII       SalI        
SapI        SbfI        ScaI        SfoI        SgrAI       
SnaBI       SpeI        Sse8387I    SspI        SwaI        
XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

Acc65I       1287  GGTACC               G^GTACC
AccI         2124  GTATAC               GT^[AC][GT]AC
AhdI          948  GACCAAGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I      1287  GGTACC               G^GTACC
AspEI         948  GACCAAGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI         1739  TGGCCA               TGG^CCA
BamHI        1152  GGATCC               G^GATCC
BlpI         2569  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     2569  GCTGAGC              GC^T[ACGT]AGC
BsiWI        1173  CGTACG               C^GTACG
BsrBI         548  CCGCTC               CCGCTC(-3/-3)
Bst1107I     2124  GTATAC               GTA^TAC
BstAPI        667  GCACTGCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI        1352  TTCGAA               TT^CGAA
BstZ17I      2124  GTATAC               GTA^TAC
CelII        2569  GCTGAGC              GC^T[ACGT]AGC
DraI          567  TTTAAA               TTT^AAA
DrdI         1208  GACCCTGGAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I      948  GACCAAGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI        1048  CCTTTGGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HindIII       703  AAGCTT               A^AGCTT
KpnI         1287  GGTACC               GGTAC^C
KspI           61  CCGCGG               CCGC^GG
MluNI        1739  TGGCCA               TGG^CCA
MscI         1739  TGGCCA               TGG^CCA
NaeI          334  GCCGGC               GCC^GGC
NcoI         1796  CCATGG               C^CATGG
NdeI         2195  CATATG               CA^TATG
NgoMIV        334  GCCGGC               G^CCGGC
NspV         1352  TTCGAA               TT^CGAA
SacII          61  CCGCGG               CCGC^GG
SexAI         860  ACCTGGT              A^CC[AT]GGT
SfiI         1252  GGCCAGCAGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI         1352  TTCGAA               TT^CGAA
SmaI         1406  CCCGGG               CCC^GGG
SmlI         1674  CTCAAG               C^T[CT][GA]AG
TfiI         1003  GATTC                G^A[AT]TC
XcmI          351  CCAGGTGGAGGATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI        1406  CCCGGG               C^CCGGG
XmaI         1406  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   46 enzymes) #
###################################

AccII          62  CGCG                 CG^CG
             1373  CGCG                
AccIII       1763  TCCGGA               T^CCGGA
             2262  TCCGGA              
AcyI          331  GACGCC               G[GA]^CG[CT]C
             2190  GACGCC              
AocI          885  CCTCAGG              CC^T[ACGT]AGG
             1695  CCTCAGG             
Aor51HI       323  AGCGCT               AGC^GCT
              970  AGCGCT              
ApaI          913  GGGCCC               GGGCC^C
             1260  GGGCCC              
AspI          156  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1393  GACAGTGTC           
BbrPI        1735  CACGTG               CAC^GTG
             2180  CACGTG              
BfaI          722  CTAG                 C^TAG
              958  CTAG                
BsaHI         331  GACGCC               G[GA]^CG[CT]C
             2190  GACGCC              
BsaI         1961  GAGACC               GGTCTC(1/5)
             1230  GGTCTC              
BseAI        1763  TCCGGA               T^CCGGA
             2262  TCCGGA              
BsgI         1247  GTGCAG               GTGCAG(16/14)
             2131  GTGCAG              
BsiEI          25  CGGCCG               CG[GA][CT]^CG
              336  CGGCCG              
Bsp1407I      266  TGTACA               T^GTACA
              772  TGTACA              
BspEI        1763  TCCGGA               T^CCGGA
             2262  TCCGGA              
BsrDI        1668  CATTGC               GCAATG(2/0)
             1914  CATTGC              
BsrGI         266  TGTACA               T^GTACA
              772  TGTACA              
BstEII        582  GGTGACC              G^GT[ACGT]ACC
             2157  GGTGACC             
BstPI         582  GGTGACC              G^GT[ACGT]ACC
             2157  GGTGACC             
BstUI          62  CGCG                 CG^CG
             1373  CGCG                
Bsu36I        885  CCTCAGG              CC^T[ACGT]AGG
             1695  CCTCAGG             
DsaI           61  CCGCGG               C^C[GA][CT]GG
             1796  CCATGG              
EagI           25  CGGCCG               C^GGCCG
              336  CGGCCG              
EclXI          25  CGGCCG               C^GGCCG
              336  CGGCCG              
Eco47III      323  AGCGCT               AGC^GCT
              970  AGCGCT              
Eco52I         25  CGGCCG               C^GGCCG
              336  CGGCCG              
Eco81I        885  CCTCAGG              CC^T[ACGT]AGG
             1695  CCTCAGG             
EcoO65I       582  GGTGACC              G^GT[ACGT]ACC
             2157  GGTGACC             
Hin1I         331  GACGCC               G[GA]^CG[CT]C
             2190  GACGCC              
MaeI          722  CTAG                 C^TAG
              958  CTAG                
MroI         1763  TCCGGA               T^CCGGA
             2262  TCCGGA              
MseI          568  TTAA                 T^TAA
             1874  TTAA                
MvnI           62  CGCG                 CG^CG
             1373  CGCG                
NspI         1833  GCATGC               [GA]CATG^[CT]
             1982  GCATGC              
PflFI         156  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1393  GACAGTGTC           
PmaCI        1735  CACGTG               CAC^GTG
             2180  CACGTG              
PmlI         1735  CACGTG               CAC^GTG
             2180  CACGTG              
PvuII        1606  CAGCTG               CAG^CTG
             2374  CAGCTG              
SacI          755  GAGCTC               GAGCT^C
             1938  GAGCTC              
SphI         1833  GCATGC               GCATG^C
             1982  GCATGC              
SspBI         266  TGTACA               T^GTACA
              772  TGTACA              
StuI          627  AGGCCT               AGG^CCT
             1008  AGGCCT              
Tru9I         568  TTAA                 T^TAA
             1874  TTAA                
Tsp509I      1138  AATT                 ^AATT
             1978  AATT                
Tth111I       156  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1393  GACAGTGTC           


#########################################
# 3 OR MORE CUT ENZYMES (   99 enzymes) #
#########################################

AciI        AfaI        AflIII      AluI        AlwI        
AlwNI       AspHI       AvaI        AvaII       BanI        
BanII       BbvCI       BbvI        BcnI        BglI        
BglII       BmyI        BpmI        BsaAI       BsaJI       
BsaWI       BseRI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmBI       BsmFI       BsoBI       Bsp1286I    
BspHI       BspMI       BsrFI       BsrI        BssKI       
BstF5I      BstNI       BstXI       BstYI       Cac8I       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DraII       EaeI        EarI        EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeII       MaeIII      MboI        MboII       MflI        
MnlI        MslI        MspA1I      MspI        MvaI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
PflMI       PleI        PpuMI       PstI        RcaI        
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        StyI        TaqI        TseI        Tsp45I      
TspRI       TthHB8I     Van91I      XhoII       
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