Restriction map for pF1KB4431
 (Linear) (Six-base) MAPSORT of: KIBB4431.seq  Check: 4233  from: 1  to: 2670



KIBB4431.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:25  ..

AccI GT'mk_AC

Cuts at:      0    2125    2670 
   Size:       2125     545
 
Acc65I G'GTAC_C

Cuts at:      0    1287    2670 
   Size:       1287    1383
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     148     331    1630    2670 
   Size:        148     183    1299    1040

  Fragments arranged by size: 

                1299    1040     183     148
 
AfeI AGC'GCT

Cuts at:      0     325     972    2670 
   Size:        325     647    1698

  Fragments arranged by size: 

                1698     647     325
 
AflIII A'CryG_T

Cuts at:      0    1176    1734    2141    2179    2279    2670 
   Size:       1176     558     407      38     100     391

  Fragments arranged by size: 

                1176     558     407     391     100      38
 
AhdI GACnn_n'nnGTC

Cuts at:      0     953    2670 
   Size:        953    1717
 
AlwNI CAG_nnn'CTG

Cuts at:      0     652     745    1224    1893    2537    2666    2670 
   Size:        652      93     479     669     644     129       4

  Fragments arranged by size: 

                 669     652     644     479     129      93       4
 
ApaI G_GGCC'C

Cuts at:      0     917    1264    2670 
   Size:        917     347    1406

  Fragments arranged by size: 

                1406     917     347
 
AvaI C'yCGr_G

Cuts at:      0      67    1039    1406    1462    2328    2670 
   Size:         67     972     367      56     866     342

  Fragments arranged by size: 

                 972     866     367     342      67      56
 
BamHI G'GATC_C

Cuts at:      0    1152    2670 
   Size:       1152    1518
 
BanI G'GyrC_C

Cuts at:      0     347     864     936    1287    2670 
   Size:        347     517      72     351    1383

  Fragments arranged by size: 

                1383     517     351     347      72
 
BanII G_rGCy'C

Cuts at:      0      35     144     759     917    1264    1942    2438    2670 
   Size:         35     109     615     158     347     678     496     232

  Fragments arranged by size: 

                 678     615     496     347     232     158     109      35
            
 
BbvCI CC'TCA_GC

Cuts at:      0       7     304    2429    2670 
   Size:          7     297    2125     241

  Fragments arranged by size: 

                2125     297     241       7
 
BglI GCCn_nnn'nGGC

Cuts at:      0      63     393    1259    2670 
   Size:         63     330     866    1411

  Fragments arranged by size: 

                1411     866     330      63
 
BglII A'GATC_T

Cuts at:      0     377     438    2036    2670 
   Size:        377      61    1598     634

  Fragments arranged by size: 

                1598     634     377      61
 
BlpI GC'TnA_GC

Cuts at:      0    2570    2670 
   Size:       2570     100
 
Bme1580I G_kGCm'C

Cuts at:      0     836     917     941    1264    2670 
   Size:        836      81      24     323    1406

  Fragments arranged by size: 

                1406     836     323      81      24
 
BmgBI CAC'GTC

Cuts at:      0    2142    2280    2670 
   Size:       2142     138     390

  Fragments arranged by size: 

                2142     390     138
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     268     942    2525    2670 
   Size:        268     674    1583     145

  Fragments arranged by size: 

                1583     674     268     145
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1641    2670 
   Size:       1641    1029
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1233    1543    1870    2122    2325    2377    2476    2670 
   Size:       1233     310     327     252     203      52      99     194

  Fragments arranged by size: 

                1233     327     310     252     203     194      99      52
            
 
Bpu10I CC'TnA_GC

Cuts at:      0       7     304    1936    2429    2670 
   Size:          7     297    1632     493     241

  Fragments arranged by size: 

                1632     493     297     241       7
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1659    2670 
   Size:       1659    1011
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1236    1955    2670 
   Size:       1236     719     715

  Fragments arranged by size: 

                1236     719     715
 
BsaAI yAC'GTr

Cuts at:      0    1177    1737    2182    2670 
   Size:       1177     560     445     488

  Fragments arranged by size: 

                1177     560     488     445
 
BsaHI Gr'CG_yC

Cuts at:      0     332    2191    2670 
   Size:        332    1859     479

  Fragments arranged by size: 

                1859     479     332
 
BsaWI w'CCGG_w

Cuts at:      0    1763    1898    2262    2670 
   Size:       1763     135     364     408

  Fragments arranged by size: 

                1763     408     364     135
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     131     161    2266    2296    2670 
   Size:        131      30    2105      30     374

  Fragments arranged by size: 

                2105     374     131      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     223    1256    2446    2670 
   Size:        223    1033    1190     224

  Fragments arranged by size: 

                1190    1033     224     223
 
BseYI C'CCAG_C

Cuts at:      0     383     803     963    1148    1604    2491    2670 
   Size:        383     420     160     185     456     887     179

  Fragments arranged by size: 

                 887     456     420     383     185     179     160
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1268    2152    2670 
   Size:       1268     884     518

  Fragments arranged by size: 

                1268     884     518
 
BsiEI CG_ry'CG

Cuts at:      0      28     339    2670 
   Size:         28     311    2331

  Fragments arranged by size: 

                2331     311      28
 
BsiHKAI G_wGCw'C

Cuts at:      0     595     759    1535    1942    2594    2670 
   Size:        595     164     776     407     652      76

  Fragments arranged by size: 

                 776     652     595     407     164      76
 
BsiWI C'GTAC_G

Cuts at:      0    1173    2670 
   Size:       1173    1497
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1191    1353    1363    1789    2670 
   Size:       1191     162      10     426     881

  Fragments arranged by size: 

                1191     881     426     162      10
 
Bsp1286I G_dGCh'C

Cuts at:      0      35     144     595     759     836     917     941    1264 
   Size:         35     109     451     164      77      81      24     323

Cuts at:   1264    1535    1942    2438    2594    2670 
   Size:        271     407     496     156      76

  Fragments arranged by size: 

                 496     451     407     323     271     164     156     109
                  81      77      76      35      24
 
BspEI T'CCGG_A

Cuts at:      0    1763    2262    2670 
   Size:       1763     499     408

  Fragments arranged by size: 

                1763     499     408
 
BspHI T'CATG_A

Cuts at:      0    1239    1814    2174    2670 
   Size:       1239     575     360     496

  Fragments arranged by size: 

                1239     575     496     360
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     112     853    1838    2670 
   Size:        112     741     985     832

  Fragments arranged by size: 

                 985     832     741     112
 
BsrBI CCG'CTC

Cuts at:      0     550    2670 
   Size:        550    2120
 
BsrDI GCAATG_nn'

Cuts at:      0    1667    1913    2670 
   Size:       1667     246     757

  Fragments arranged by size: 

                1667     757     246
 
BsrFI r'CCGG_y

Cuts at:      0     174     334     338    2670 
   Size:        174     160       4    2332

  Fragments arranged by size: 

                2332     174     160       4
 
BsrGI T'GTAC_A

Cuts at:      0     266     772    2670 
   Size:        266     506    1898

  Fragments arranged by size: 

                1898     506     266
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     673    2670 
   Size:        673    1997
 
BstBI TT'CG_AA

Cuts at:      0    1353    2670 
   Size:       1353    1317
 
BstEII G'GTnAC_C

Cuts at:      0     582    2157    2670 
   Size:        582    1575     513

  Fragments arranged by size: 

                1575     582     513
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     803     909    1797    2670 
   Size:        803     106     888     873

  Fragments arranged by size: 

                 888     873     803     106
 
BstYI r'GATC_y

Cuts at:      0     377     438    1152    2036    2670 
   Size:        377      61     714     884     634

  Fragments arranged by size: 

                 884     714     634     377      61
 
BstZ17I GTA'TAC

Cuts at:      0    2126    2670 
   Size:       2126     544
 
Bsu36I CC'TnA_GG

Cuts at:      0     886    1696    2670 
   Size:        886     810     974

  Fragments arranged by size: 

                 974     886     810
 
BtgI C'CryG_G

Cuts at:      0      61    1796    2670 
   Size:         61    1735     874

  Fragments arranged by size: 

                1735     874      61
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1517    2670 
   Size:       1517    1153
 
BtsI GCAGTG_nn'

Cuts at:      0     198     452     667    2438    2508    2522    2670 
   Size:        198     254     215    1771      70      14     148

  Fragments arranged by size: 

                1771     254     215     198     148      70      14
 
DraI TTT'AAA

Cuts at:      0     569    2670 
   Size:        569    2101
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1214    2670 
   Size:       1214    1456
 
EaeI y'GGCC_r

Cuts at:      0      25     336     750    1739    2111    2670 
   Size:         25     311     414     989     372     559

  Fragments arranged by size: 

                 989     559     414     372     311      25
 
EagI C'GGCC_G

Cuts at:      0      25     336    2670 
   Size:         25     311    2334

  Fragments arranged by size: 

                2334     311      25
 
EarI CTCTTCn'nnn_

Cuts at:      0     642    1239    1682    2670 
   Size:        642     597     443     988

  Fragments arranged by size: 

                 988     642     597     443
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      27     102    2670 
   Size:         27      75    2568

  Fragments arranged by size: 

                2568      75      27
 
EcoICRI GAG'CTC

Cuts at:      0     757    1940    2670 
   Size:        757    1183     730

  Fragments arranged by size: 

                1183     757     730
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     148     331    1233    1543    1630    1870    2122    2325 
   Size:        148     183     902     310      87     240     252     203

Cuts at:   2325    2377    2476    2670 
   Size:         52      99     194

  Fragments arranged by size: 

                 902     310     252     240     203     194     183     148
                  99      87      52
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1052    2670 
   Size:       1052    1618
 
EcoO109I rG'GnC_Cy

Cuts at:      0     490     544     792     858     913     914    1044    1057 
   Size:        490      54     248      66      55       1     130      13

Cuts at:   1057    1066    1164    1260    1261    1827    2400    2670 
   Size:          9      98      96       1     566     573     270

  Fragments arranged by size: 

                 573     566     490     270     248     130      98      96
                  66      55      54      13       9       1       1
 
HaeII r_GCGC'y

Cuts at:      0       7     327     974    2328    2670 
   Size:          7     320     647    1354     342

  Fragments arranged by size: 

                1354     647     342     320       7
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     147     179     939     971    2670 
   Size:        147      32     760      32    1699

  Fragments arranged by size: 

                1699     760     147      32      32
 
HindIII A'AGCT_T

Cuts at:      0     703    2670 
   Size:        703    1967
 
KpnI G_GTAC'C

Cuts at:      0    1291    2670 
   Size:       1291    1379
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     492    2670 
   Size:        492    2178
 
MscI TGG'CCA

Cuts at:      0    1741    2670 
   Size:       1741     929
 
MslI CAynn'nnrTG

Cuts at:      0     694    1244    1795    2002    2670 
   Size:        694     550     551     207     668

  Fragments arranged by size: 

                 694     668     551     550     207
 
MspA1I CmG'CkG

Cuts at:      0      42      48      63    1608    2376    2670 
   Size:         42       6      15    1545     768     294

  Fragments arranged by size: 

                1545     768     294      42      15       6
 
NaeI GCC'GGC

Cuts at:      0     336    2670 
   Size:        336    2334
 
NcoI C'CATG_G

Cuts at:      0    1796    2670 
   Size:       1796     874
 
NdeI CA'TA_TG

Cuts at:      0    2196    2670 
   Size:       2196     474
 
NgoMIV G'CCGG_C

Cuts at:      0     334    2670 
   Size:        334    2336
 
NspI r_CATG'y

Cuts at:      0    1837    1986    2670 
   Size:       1837     149     684

  Fragments arranged by size: 

                1837     684     149
 
PasI CC'CwG_GG

Cuts at:      0    1062    1557    2670 
   Size:       1062     495    1113

  Fragments arranged by size: 

                1113    1062     495
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     255     515    2200    2670 
   Size:        255     260    1685     470

  Fragments arranged by size: 

                1685     470     260     255
 
PfoI T'CCnGG_A

Cuts at:      0     104     854    1852    2029    2670 
   Size:        104     750     998     177     641

  Fragments arranged by size: 

                 998     750     641     177     104
 
PmlI CAC'GTG

Cuts at:      0    1737    2182    2670 
   Size:       1737     445     488

  Fragments arranged by size: 

                1737     488     445
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1534    1566    2670 
   Size:       1534      32    1104

  Fragments arranged by size: 

                1534    1104      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     490     544     858    1066    1164    1827    2400    2670 
   Size:        490      54     314     208      98     663     573     270

  Fragments arranged by size: 

                 663     573     490     314     270     208      98      54
            
 
PspOMI G'GGCC_C

Cuts at:      0     913    1260    2670 
   Size:        913     347    1410

  Fragments arranged by size: 

                1410     913     347
 
PstI C_TGCA'G

Cuts at:      0     193     678    2642    2670 
   Size:        193     485    1964      28

  Fragments arranged by size: 

                1964     485     193      28
 
PvuII CAG'CTG

Cuts at:      0    1608    2376    2670 
   Size:       1608     768     294

  Fragments arranged by size: 

                1608     768     294
 
SacI G_AGCT'C

Cuts at:      0     759    1942    2670 
   Size:        759    1183     728

  Fragments arranged by size: 

                1183     759     728
 
SacII CC_GC'GG

Cuts at:      0      64    2670 
   Size:         64    2606
 
SanDI GG'GwC_CC

Cuts at:      0     490     544    1066    2670 
   Size:        490      54     522    1604

  Fragments arranged by size: 

                1604     522     490      54
 
SexAI A'CCwGG_T

Cuts at:      0     860    2670 
   Size:        860    1810
 
SfcI C'TryA_G

Cuts at:      0     189     674     763     879    2040    2481    2638    2670 
   Size:        189     485      89     116    1161     441     157      32

  Fragments arranged by size: 

                1161     485     441     189     157     116      89      32
            
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    1259    2670 
   Size:       1259    1411
 
SmaI CCC'GGG

Cuts at:      0    1408    2670 
   Size:       1408    1262
 
SmlI C'TyrA_G

Cuts at:      0    1674    2670 
   Size:       1674     996
 
SphI G_CATG'C

Cuts at:      0    1837    1986    2670 
   Size:       1837     149     684

  Fragments arranged by size: 

                1837     684     149
 
StuI AGG'CCT

Cuts at:      0     629    1010    2670 
   Size:        629     381    1660

  Fragments arranged by size: 

                1660     629     381
 
StyI C'CwwG_G

Cuts at:      0     908     950    1299    1796    2670 
   Size:        908      42     349     497     874

  Fragments arranged by size: 

                 908     874     497     349      42
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     216     468     924    2509    2670 
   Size:        216     252     456    1585     161

  Fragments arranged by size: 

                1585     456     252     216     161
 
TatI w'GTAC_w

Cuts at:      0     266     772    2670 
   Size:        266     506    1898

  Fragments arranged by size: 

                1898     506     266
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     131     163     726     758    2670 
   Size:        131      32     563      32    1912

  Fragments arranged by size: 

                1912     563     131      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     159    1396    2670 
   Size:        159    1237    1274

  Fragments arranged by size: 

                1274    1237     159
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     358    2670 
   Size:        358    2312
 
XmaI C'CCGG_G

Cuts at:      0    1406    2670 
   Size:       1406    1264
 

 Enzymes that do cut:

     AccI   Acc65I     AcuI     AfeI   AflIII     AhdI    AlwNI     ApaI
     AvaI    BamHI     BanI    BanII    BbvCI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BstAPI    BstBI   BstEII    BstXI    BstYI
  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     DraI     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    HaeII
    Hin4I  HindIII     KpnI     MmeI     MscI     MslI   MspA1I     NaeI
     NcoI     NdeI   NgoMIV     NspI     PasI    PflMI     PfoI     PmlI
     PpiI    PpuMI   PspOMI     PstI    PvuII     SacI    SacII    SanDI
    SexAI     SfcI     SfiI     SmaI     SmlI     SphI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AclI    AflII     AgeI     AleI     AlfI     AloI
    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI     BbeI
     BbsI     BcgI    BciVI     BclI    BfrBI     BmtI    BsaBI     BsmI
   BssHII    BssSI     ClaI    CspCI   DraIII    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI   HincII     HpaI     KasI     MfeI     MluI
     NarI     NheI     NotI     NruI     NsiI     PacI     PciI     PmeI
    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII     SalI     SapI
     SbfI     ScaI     SfoI     SgfI    SgrAI    SnaBI     SpeI     SrfI
     SspI     SwaI     XbaI     XhoI     XmnI     ZraI
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