Restriction map for pF1KB4421
##################################
# NO CUT ENZYMES (  137 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AflII       AgeI        AhdI        Alw44I      AlwNI       
AocI        Aor51HI     ApaI        ApaLI       AscI        
Asp718I     AspEI       AspI        AvaI        AviII       
AvrII       BamHI       BbeI        BbrPI       BcgI        
BciVI       BclI        BfrI        BglI        BlnI        
BlpI        Bpu1102I    BsaAI       BsaBI       BsaHI       
BsaWI       BseAI       BsgI        BsiEI       BsiWI       
BsmBI       BsmI        BsoBI       Bsp1407I    BspDI       
BspEI       BspLU11I    BsrBI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstZ17I     Bsu36I      CelII       
CfoI        Cfr10I      ClaI        CpoI        DraII       
DraIII      DrdI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRI       EcoRV       EcoT22I     FbaI        
FseI        FspI        HaeII       HhaI        Hin1I       
HinP1I      HpaI        KasI        KpnI        KspI        
MamI        MluI        MroI        MspA1I      NaeI        
NarI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
Psp1406I    PstI        PvuI        PvuII       RsrII       
SacI        SacII       SapI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SphI        Sse8387I    SspBI       
StuI        SwaI        Tth111I     XbaI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AccI         1174  GTCGAC               GT^[AC][GT]AC
AccII         111  CGCG                 CG^CG
AflIII       1159  ACACGT               A^C[GA][CT]GT
AlwI          638  GGATC                GGATC(4/5)
AseI          843  ATTAAT               AT^TAAT
AsnI          843  ATTAAT               AT^TAAT
BalI          804  TGGCCA               TGG^CCA
BanI         1479  GGCACC               G^G[CT][GA]CC
BbsI          574  GAAGAC               GAAGAC(2/6)
BbvCI          61  GCTGAGG              CCTCAGC(-5/-2)
BcnI           39  CCCGG                CC^[GC]GG
BfaI          230  CTAG                 C^TAG
BglII         963  AGATCT               A^GATCT
BpuAI         574  GAAGAC               GAAGAC(2/6)
BsaI          650  GAGACC               GGTCTC(1/5)
BspHI         732  TCATGA               T^CATGA
BspMI        1559  ACCTGC               ACCTGC(4/8)
BsrDI         382  GCAATG               GCAATG(2/0)
BstUI         111  CGCG                 CG^CG
BstXI         608  CCATGGCTTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         963  AGATCT               [GA]^GATC[CT]
DraI         1105  TTTAAA               TTT^AAA
HincII       1174  GTCGAC               GT[CT]^[GA]AC
HindII       1174  GTCGAC               GT[CT]^[GA]AC
HindIII      1526  AAGCTT               A^AGCTT
MaeI          230  CTAG                 C^TAG
MaeII        1161  ACGT                 A^CGT
MfeI          902  CAATTG               C^AATTG
MflI          963  AGATCT               [GA]^GATC[CT]
MluNI         804  TGGCCA               TGG^CCA
MscI          804  TGGCCA               TGG^CCA
MunI          902  CAATTG               C^AATTG
MvnI          111  CGCG                 CG^CG
NciI           39  CCCGG                CC^[GC]GG
NspI          854  ACATGC               [GA]CATG^[CT]
PflMI         874  CCATTCACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI         1189  GACTC                GAGTC(4/5)
PshAI        1167  GACACCTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI         843  ATTAAT               AT^TAAT
RcaI          732  TCATGA               T^CATGA
SalI         1174  GTCGAC               G^TCGAC
SfcI          645  CTACAG               C^T[GA][CT]AG
SmlI          514  CTTGAG               C^T[CT][GA]AG
SpeI          229  ACTAGT               A^CTAGT
Van91I        874  CCATTCACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          165  CCATGGGGGAGTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         963  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AciI           18  GCGG                 CCGC(-3/-1)
              112  GCGG                
AcsI          211  AAATTT               [GA]^AATT[CT]
             1348  AAATTT              
ApoI          211  AAATTT               [GA]^AATT[CT]
             1348  AAATTT              
Asp700I       724  GAATCTATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1072  GAAGAACTTC          
AspHI         866  GAGCAC               G[AT]GC[AT]^C
             1555  GAGCAC              
BanII         114  GGGCTC               G[GA]GC[CT]^C
             1096  GAGCCC              
BseRI         133  GAGGAG               GAGGAG(10/8)
              530  GAGGAG              
BsiHKAI       866  GAGCAC               G[AT]GC[AT]^C
             1555  GAGCAC              
BsmAI          50  GAGAC                GTCTC(1/5)
              650  GAGAC               
BsmFI          20  GGGAC                GGGAC(10/14)
             1022  GGGAC               
DsaI          165  CCATGG               C^C[GA][CT]GG
              608  CCATGG              
EarI         1126  GAAGAG               CTCTTC(1/4)
             1373  GAAGAG              
FauI           18  GCGGG                CCCGC(4/6)
              112  GCGGG               
HapII          40  CCGG                 C^CGG
              661  CCGG                
HgaI          109  GACGC                GACGC(5/10)
             1024  GACGC               
HpaII          40  CCGG                 C^CGG
              661  CCGG                
HphI          681  TCACC                GGTGA(8/7)
             1300  TCACC               
Ksp632I      1126  GAAGAG               CTCTTC(1/4)
             1373  GAAGAG              
MslI          363  CATGAAGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              808  CACAATCGTG          
MspI           40  CCGG                 C^CGG
              661  CCGG                
MwoI          969  GCTTAAAAAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
             1387  GCCAGCACAGC         
NcoI          165  CCATGG               C^CATGG
              608  CCATGG              
NdeI          182  CATATG               CA^TATG
             1235  CATATG              
SfaNI         453  GCATC                GCATC(5/9)
             1474  GCATC               
Tsp45I        745  GTCAC                ^GT[GC]AC
             1456  GTGAC               
XmnI          724  GAATCTATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1072  GAAGAACTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   48 enzymes) #
#########################################

AfaI        AluI        AvaII       BbvI        BmyI        
BpmI        BsaJI       BsiYI       BslI        Bsp1286I    
BsrI        BssKI       BstF5I      BstNI       Cac8I       
Cfr13I      DdeI        DpnII       EaeI        EcoRII      
EcoT14I     Fnu4HI      FokI        HaeIII      HinfI       
ItaI        MaeIII      MboI        MboII       MnlI        
MseI        MvaI        NdeII       NlaIII      NlaIV       
RsaI        Sau3AI      Sau96I      ScrFI       SspI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp509I     TspRI       TthHB8I     
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com